SEQUENCE LISTING

<110> PROTIVA BIOTHERAPEUTICS, INC.
 
<120> COMPOSITIONS AND METHODS FOR SILENCING HEPATITIS B VIRUS GENE 
      EXPRESSION

<130> 08155.035US1

<140> 15/515,952
<141> 2017-03-30

<150> PCT/US2015/053569
<151> 2015-10-01

<150> 62/120,149
<151> 2015-02-24

<150> 62/059,056
<151> 2014-10-02

<160> 31    

<170> PatentIn version 3.5

<210> 1
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (2)..(2)
<223> 2'-O-methyl modification 

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (7)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (14)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 1
agguauguug cccguuuguu u                                                 21


<210> 2
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (7)..(7)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 2
acaaacgggc aacauaccuu u                                                 21


<210> 3
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (3)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 3
gcucaguuua cuagugccau u                                                 21


<210> 4
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (10)..(10)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<400> 4
uggcacuagu aaacugagcu u                                                 21


<210> 5
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (5)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 5
ccgugugcac uucgcuucau u                                                 21


<210> 6
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 6
ugaagcgaag ugcacacggu u                                                 21


<210> 7
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (3)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 7
gcucaguuua cuagugccau u                                                 21


<210> 8
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (10)..(10)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 8
uggcacuagu aaacugagcu u                                                 21


<210> 9
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (5)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 9
ccgugugcac uucgcuucau u                                                 21


<210> 10
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<400> 10
ugaagcgaag ugcacacggu u                                                 21


<210> 11
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (2)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 11
cuggcucagu uuacuagugu u                                                 21


<210> 12
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<400> 12
cacuaguaaa cugagccagu u                                                 21


<210> 13
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (5)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 13
ccgugugcac uucgcuucau u                                                 21


<210> 14
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 14
ugaagcgaag ugcacacggu u                                                 21


<210> 15
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (3)..(3)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (14)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 15
gcucaguuua cuagugccau u                                                 21


<210> 16
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 16
uggcacuagu aaacugagcu u                                                 21


<210> 17
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (2)..(2)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (14)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(16)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 17
agguauguug cccguuuguu u                                                 21


<210> 18
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (7)..(7)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (19)..(19)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 18
acaaacgggc aacauaccuu u                                                 21


<210> 19
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 19
gccgauccau acugcggaau u                                                 21


<210> 20
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 20
uuccgcagua uggaucggcu u                                                 21


<210> 21
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (16)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 21
gccgauccau acugcggaau u                                                 21


<210> 22
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<400> 22
uuccgcagua uggaucggcu u                                                 21


<210> 23
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 23
gccgauccau acugcggaau u                                                 21


<210> 24
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<400> 24
uuccgcagua uggaucggcu u                                                 21


<210> 25
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (4)..(4)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (17)..(17)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 25
gccgauccau acugcggaau u                                                 21


<210> 26
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (8)..(8)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (13)..(13)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (18)..(18)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 26
uuccgcagua uggaucggcu u                                                 21


<210> 27
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (3)..(3)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (14)..(14)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (21)..(21)
<223> UNA moiety 

<400> 27
gcucaguuua cuagugccau u                                                 21


<210> 28
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<400> 28
uggcacuagu aaacugagcu u                                                 21


<210> 29
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (1)..(1)
<223> UNA moiety 

<220>
<221> modified_base
<222> (2)..(3)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (6)..(6)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (12)..(12)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 29
cuggcucagu uuacuagugu u                                                 21


<210> 30
<211> 21
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (15)..(15)
<223> 2'-O-methyl modification

<220>
<221> modified_base
<222> (20)..(21)
<223> UNA moiety 

<400> 30
cacuaguaaa cugagccagu u                                                 21


<210> 31
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide

<400> 31
gaaggccaga cgcgaauuat t                                                 21