LISTADO DE SECUENCIAS <110> EL CONSEJO ASESOR DE LA UNIVERSIDAD DE COLUMBIA EN LA CIUDAD DE NUEVA YORK UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER <120> CONSTRUCCIONES DE VECTORES VIRALES ADENO-ASOCIADOS DE GLUT1 RECOMBINANTES Y MÉTODOS RELACIONADOS PARA RESTABLECER LA EXPRESIÓN DE GLUT1 <130> 01001/003887-WO0 <140> <141> <150> 62/130,899 <151> 2015-03-10 <160> 97 <170> PatentIn versión 3.5 <210> 1 <211> 7109 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI EGFP-2A-hGlut1 <400> 1 gccttaatta ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 60 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 120 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacc 180 agggtaatgg ggatcctcta gaactatagc tagtcgacat tgattattga ctagttatta 240 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 300 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 360 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 420 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 480 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 540 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgtcgag 600 gccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta 660 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggcgc gcgccaggcg 720 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 780 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 840 aaaagcgaag cgcgcggcgg gcgggagcaa gctttattgc ggtagtttat cacagttaaa 900 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt 960 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga 1020 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta 1080 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt 1140 aaggctagag tacttaatac gactcactat aggctagtaa tacgactcac tatagatggt 1200 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 1260 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 1320 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 1380 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 1440 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 1500 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 1560 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 1620 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 1680 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 1740 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 1800 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 1860 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaattttga 1920 ccttcttaag cttgcgggag acgtcgagtc caaccctggg cccatggagc ccagcagcaa 1980 gaagctgacg ggtcgcctca tgctggccgt gggaggagca gtgcttggct ccctgcagtt 2040 tggctacaac actggagtca tcaatgcccc ccagaaggtg atcgaggagt tctacaacca 2100 gacatgggtc caccgctatg gggagagcat cctgcccacc acgctcacca cgctctggtc 2160 cctctcagtg gccatctttt ctgttggggg catgattggc tccttctctg tgggcctttt 2220 cgttaaccgc tttggccggc ggaattcaat gctgatgatg aacctgctgg ccttcgtgtc 2280 cgccgtgctc atgggcttct cgaaactggg caagtccttt gagatgctga tcctgggccg 2340 cttcatcatc ggtgtgtact gtggcctgac cacaggcttc gtgcccatgt atgtgggtga 2400 agtgtcaccc acagcccttc gtggggccct gggcaccctg caccagctgg gcatcgtcgt 2460 cggcatcctc atcgcccagg tgttcggcct ggactccatc atgggcaaca aggacctgtg 2520 gcccctgctg ctgagcatca tcttcatccc ggccctgctg cagtgcatcg tgctgccctt 2580 ctgccccgag agtccccgct tcctgctcat caaccgcaac gaggagaacc gggccaagag 2640 tgtgctaaag aagctgcgcg ggacagctga cgtgacccat gacctgcagg agatgaagga 2700 agagagtcgg cagatgatgc gggagaagaa ggtcaccatc ctggagctgt tccgctcccc 2760 cgcctaccgc cagcccatcc tcatcgctgt ggtgctgcag ctgtcccagc agctgtctgg 2820 catcaacgct gtcttctatt actccacgag catcttcgag aaggcggggg tgcagcagcc 2880 tgtgtatgcc accattggct ccggtatcgt caacacggcc ttcactgtcg tgtcgctgtt 2940 tgtggtggag cgagcaggcc ggcggaccct gcacctcata ggcctcgctg gcatggcggg 3000 ttgtgccata ctcatgacca tcgcgctagc actgctggag cagctacccc ggatgtccta 3060 tctgagcatc gtggccatct ttggctttgt ggccttcttt gaagtgggtc ctggccccat 3120 cccatggttc atcgtggctg aactcttcag ccagggtcca cgtccagctg ccattgccgt 3180 tgcaggcttc tccaactgga cctcaaattt cattgtgggc atgtgcttcc agtatgtgga 3240 gcaactgtgt ggtccctacg tcttcatcat cttcactgtg ctcctggttc tgttcttcat 3300 cttcacctac ttcaaagttc ctgagactaa aggccggacc ttcgatgaga tcgcttccgg 3360 cttccggcag gggggagcca gccaaagtga caagacaccc gaggagctgt tccatcccct 3420 gggggctgat tcccaagtgt gagtcgcccc agatcaccag cccggcctgc tcccagcagc 3480 cctaaggatc tctcaggagc acaggcagct ggatgagact tccaaacctg acagatgtca 3540 gccgagccgg gcctggggct cctttctcca gccagcaatg atgtccagaa gaatattcag 3600 gacttaacgg ctccaggatt ttaacaaaag caagactgtt gctcaaatct attcagacaa 3660 gcaacaggtt ttataatttt tttattactg attttgttat ttttatatca gcctgagtct 3720 cctgtgccca catcccaggc ttcaccctga atggttccat gcctgagggt ggagactaag 3780 ccctgtcgag acacttgcct tcttcaccca gctaatctgt agggctggac ctatgtccta 3840 aggacacact aatcgaacta tgaactacaa agcttctatc ccaggaggtg gctatggcca 3900 cccgttctgc tggcctggat ctctcgagga cggggtgaac tacgcctgag gatccgatct 3960 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 4020 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 4080 cggaagcaat tcgttgatct gaatttcgac cacccataat acccattacc ctggtagata 4140 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc 4200 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 4260 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac 4320 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 4380 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 4440 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 4500 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4560 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4620 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4680 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4740 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4800 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4860 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4920 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 4980 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 5040 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 5100 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 5160 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 5220 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 5280 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 5340 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 5400 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 5460 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 5520 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 5580 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 5640 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 5700 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 5760 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 5820 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 5880 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 5940 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 6000 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 6060 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 6120 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 6180 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 6240 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 6300 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 6360 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 6420 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 6480 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 6540 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 6600 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 6660 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 6720 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 6780 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 6840 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 6900 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 6960 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 7020 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 7080 tgaccatgat tacgccagat ttaattaag 7109 <210> 2 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 2 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 3 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 3 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 4 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 4 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 5 <211> 717 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> eGFP <400> 5 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 6 <211> 51 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: oligonucleótido sintético <220> <223> 2A-enlazador <400> 6 aattttgacc ttcttaagct tgcgggagac gtcgagtcca accctgggcc c 51 <210> 7 <211> 1959 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 y 3'UTR <400> 7 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtgag tcgccccaga tcaccagccc 1500 ggcctgctcc cagcagccct aaggatctct caggagcaca ggcagctgga tgagacttcc 1560 aaacctgaca gatgtcagcc gagccgggcc tggggctcct ttctccagcc agcaatgatg 1620 tccagaagaa tattcaggac ttaacggctc caggatttta acaaaagcaa gactgttgct 1680 caaatctatt cagacaagca acaggtttta taattttttt attactgatt ttgttatttt 1740 tatatcagcc tgagtctcct gtgcccacat cccaggcttc accctgaatg gttccatgcc 1800 tgagggtgga gactaagccc tgtcgagaca cttgccttct tcacccagct aatctgtagg 1860 gctggaccta tgtcctaagg acacactaat cgaactatga actacaaagc ttctatccca 1920 ggaggtggct atggccaccc gttctgctgg cctggatct 1959 <210> 8 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal poli A <400> 8 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 9 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 9 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 10 <211> 7503 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI EGFP-2A-mGlut1 <400> 10 gccttaatta ggctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt 60 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 120 aactccatca ctaggggttc cttgtagtta atgattaacc cgccatgcta cttatctacc 180 agggtaatgg ggatcctcta gaactatagc tagtcgacat tgattattga ctagttatta 240 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 300 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 360 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 420 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 480 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 540 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgtcgag 600 gccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta 660 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggcgc gcgccaggcg 720 gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 780 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 840 aaaagcgaag cgcgcggcgg gcgggagcaa gctttattgc ggtagtttat cacagttaaa 900 ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agaagttggt 960 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga 1020 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta 1080 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt 1140 aaggctagag tacttaatac gactcactat aggctagtaa tacgactcac tatagatggt 1200 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 1260 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 1320 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 1380 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 1440 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 1500 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 1560 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 1620 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 1680 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 1740 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 1800 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 1860 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agaattttga 1920 ccttcttaag cttgcgggag acgtcgagtc caaccctggg cccatggatc ccagcagcaa 1980 gaaggtgacg ggccgcctca tgttggctgt gggaggagca gtgctcggat cactgcagtt 2040 cggctataac actggtgtca tcaacgcccc ccagaaggtt attgaggagt tctacaatca 2100 aacatggaac caccgctacg gagagcccat cccatccacc acactcacca cgctttggtc 2160 tctctccgtg gccatcttct ctgtcggggg catgattggt tccttctctg tcggcctctt 2220 tgttaatcgc tttggcaggc ggaactccat gctgatgatg aacctgttgg cctttgtggc 2280 tgctgtgctt atgggcttct ccaaactggg caagtccttt gagatgctga tcctgggccg 2340 cttcatcatc ggtgtgtact gcggcctgac tactggcttt gtgcccatgt atgtgggaga 2400 ggtgtcacct acagctctac gtggagccct aggcacactg caccagctgg gaatcgtcgt 2460 tggcatcctt attgcccagg tgtttggctt agactccatc atgggcaatg cagacttgtg 2520 gcctctgctg ctcagtgtca tcttcatccc agccctgcta cagtgtatcc tgttgccctt 2580 ctgccccgag agcccccgct tcctgctcat caatcgtaac gaggagaacc gggccaagag 2640 tgtgctgaag aagcttcgag ggacagccga tgtgacccga gacctgcagg agatgaaaga 2700 agagggtcgg cagatgatgc gggagaagaa ggtcaccatc ttggagctgt tccgctcacc 2760 cgcctaccgc cagcccatcc tcatcgctgt ggtgctgcag ctgtcccagc agctgtcggg 2820 tatcaatgct gtgttctact actcaacgag catcttcgag aaggcaggtg tgcagcagcc 2880 tgtgtacgcc accatcggct ccggtatcgt caacacggcc ttcactgtgg tgtcgctgtt 2940 tgttgtagag cgagctggac gacggaccct gcacctcatt ggcctggctg gcatggcagg 3000 ctgtgctgtg ctcatgacca tcgccctggc cttgctggaa cggctgcctt ggatgtccta 3060 tctgagcatc gtggccatct ttggctttgt ggccttcttt gaagtaggcc ctggtcctat 3120 tccatggttc attgtggccg agctgttcag ccaggggccc cgtcctgctg ctattgctgt 3180 ggctggcttc tccaactgga cctcaaactt cattgtgggc atgtgcttcc agtatgtgga 3240 gcaactgtgc ggcccctacg tcttcatcat cttcacggtg ctcctcgtgc tcttcttcat 3300 cttcacctac ttcaaagtcc ctgagaccaa aggccgaacc ttcgatgaga tcgcttccgg 3360 cttccggcag gggggtgcca gccaaagtga caagacaccc gaggagctct tccaccctct 3420 gggggcggac tcccaagtgt gaggagcccc acacccagcc cggcctgctc cctgcagccc 3480 aaggatctct ctggagcaca ggcagctaga tgagacctct tccgaaccga cagatctcgg 3540 gcaagccggg cctgggcgcc tttcctcagc cagcagtgaa gtccaggagg atattcagga 3600 ctttgatggc tccagaattt ttaatgaaag caagactgct gctcagatct attcagataa 3660 gcagcaggtt ttataatttt tttattactg attttgttat tttttttttt tatcagccac 3720 tctcctatct ccacactgta gtcttcacct tgattggccc agtgcctgag ggtggggacc 3780 acgccctgtc cagacacttg ccttctttgc caagctaatc tgtagggctg gacctatggc 3840 caaggacaca ctaataccga actctgagct aggaggcttt accgctggag gcggtagctg 3900 ccacccactt ccgcaggcct ggacctcggc accatagggg tccggactcc attttaggat 3960 tcgcccattc ctgtctcttc ctacccaacc actcaattaa tctttccttg cctgagacca 4020 gttggaagca ctggagtgca gggaggagag ggaagggcca ggctgggctg ccaggttcta 4080 gtctcctgtg cactgagggc cacacaaaca ccatgagaag gacctcggag gctgagaact 4140 taactgctga agacacggac actcctgccc tgctgtgtat agatggaaga tatttatata 4200 ttttttggtt gtcaatatta aatacagaca ctaagttata gtatatctgg acaaacccac 4260 ttgtaaatac accaacaaac tcctgtaact ttacctaagc agatataaat ggctggctcg 4320 aggacggggt gaactacgcc tgaggatccg atctttttcc ctctgccaaa aattatgggg 4380 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 4440 caatagtgtg ttggaatttt ttgtgtctct cactcggaag caattcgttg atctgaattt 4500 cgaccaccca taatacccat taccctggta gataagtagc atggcgggtt aatcattaac 4560 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 4620 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 4680 gagcgagcgc gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac 4740 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 4800 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 4860 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 4920 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 4980 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 5040 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 5100 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 5160 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 5220 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 5280 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc 5340 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 5400 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 5460 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5520 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5580 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5640 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5700 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5760 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5820 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5880 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5940 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 6000 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 6060 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 6120 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6180 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6240 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6300 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 6360 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6420 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6480 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6540 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6600 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 6660 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6720 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6780 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6840 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6900 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6960 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 7020 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 7080 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 7140 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 7200 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 7260 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 7320 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca 7380 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 7440 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt 7500 aag 7503 <210> 11 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 11 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 12 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 12 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 13 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 13 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 14 <211> 717 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> eGFP <400> 14 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 15 <211> 51 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: oligonucleótido sintético <220> <223> 2A-enlazador <400> 15 aattttgacc ttcttaagct tgcgggagac gtcgagtcca accctgggcc c 51 <210> 16 <211> 2353 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de mGlut1 y 3'UTR <400> 16 atggatccca gcagcaagaa ggtgacgggc cgcctcatgt tggctgtggg aggagcagtg 60 ctcggatcac tgcagttcgg ctataacact ggtgtcatca acgcccccca gaaggttatt 120 gaggagttct acaatcaaac atggaaccac cgctacggag agcccatccc atccaccaca 180 ctcaccacgc tttggtctct ctccgtggcc atcttctctg tcgggggcat gattggttcc 240 ttctctgtcg gcctctttgt taatcgcttt ggcaggcgga actccatgct gatgatgaac 300 ctgttggcct ttgtggctgc tgtgcttatg ggcttctcca aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgcg gcctgactac tggctttgtg 420 cccatgtatg tgggagaggt gtcacctaca gctctacgtg gagccctagg cacactgcac 480 cagctgggaa tcgtcgttgg catccttatt gcccaggtgt ttggcttaga ctccatcatg 540 ggcaatgcag acttgtggcc tctgctgctc agtgtcatct tcatcccagc cctgctacag 600 tgtatcctgt tgcccttctg ccccgagagc ccccgcttcc tgctcatcaa tcgtaacgag 660 gagaaccggg ccaagagtgt gctgaagaag cttcgaggga cagccgatgt gacccgagac 720 ctgcaggaga tgaaagaaga gggtcggcag atgatgcggg agaagaaggt caccatcttg 780 gagctgttcc gctcacccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtcgggtat caatgctgtg ttctactact caacgagcat cttcgagaag 900 gcaggtgtgc agcagcctgt gtacgccacc atcggctccg gtatcgtcaa cacggccttc 960 actgtggtgt cgctgtttgt tgtagagcga gctggacgac ggaccctgca cctcattggc 1020 ctggctggca tggcaggctg tgctgtgctc atgaccatcg ccctggcctt gctggaacgg 1080 ctgccttgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtaggccctg gtcctattcc atggttcatt gtggccgagc tgttcagcca ggggccccgt 1200 cctgctgcta ttgctgtggc tggcttctcc aactggacct caaacttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgcggc ccctacgtct tcatcatctt cacggtgctc 1320 ctcgtgctct tcttcatctt cacctacttc aaagtccctg agaccaaagg ccgaaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggtgccagcc aaagtgacaa gacacccgag 1440 gagctcttcc accctctggg ggcggactcc caagtgtgag gagccccaca cccagcccgg 1500 cctgctccct gcagcccaag gatctctctg gagcacaggc agctagatga gacctcttcc 1560 gaaccgacag atctcgggca agccgggcct gggcgccttt cctcagccag cagtgaagtc 1620 caggaggata ttcaggactt tgatggctcc agaattttta atgaaagcaa gactgctgct 1680 cagatctatt cagataagca gcaggtttta taattttttt attactgatt ttgttatttt 1740 ttttttttat cagccactct cctatctcca cactgtagtc ttcaccttga ttggcccagt 1800 gcctgagggt ggggaccacg ccctgtccag acacttgcct tctttgccaa gctaatctgt 1860 agggctggac ctatggccaa ggacacacta ataccgaact ctgagctagg aggctttacc 1920 gctggaggcg gtagctgcca cccacttccg caggcctgga cctcggcacc ataggggtcc 1980 ggactccatt ttaggattcg cccattcctg tctcttccta cccaaccact caattaatct 2040 ttccttgcct gagaccagtt ggaagcactg gagtgcaggg aggagaggga agggccaggc 2100 tgggctgcca ggttctagtc tcctgtgcac tgagggccac acaaacacca tgagaaggac 2160 ctcggaggct gagaacttaa ctgctgaaga cacggacact cctgccctgc tgtgtataga 2220 tggaagatat ttatatattt tttggttgtc aatattaaat acagacacta agttatagta 2280 tatctggaca aacccacttg taaatacacc aacaaactcc tgtaacttta cctaagcaga 2340 tataaatggc tgg 2353 <210> 17 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 17 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 18 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 18 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 19 <211> 6844 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI hGlut1-2A-EGFP <400> 19 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattgatc cactagtaac 1200 ggccgccagt gtgctggaag caggagacca aacgacgggg gtcggagtca gagtcgcagt 1260 gggagtcccc ggaccggagc acgagcctga gcgggagagc gccgctcgca cgcccgtcgc 1320 cacccgcgta cccggcgcag ccagagccac cagcgcagcg ctgccatgga gcccagcagc 1380 aagaagctga cgggtcgcct catgctggcc gtgggaggag cagtgcttgg ctccctgcag 1440 tttggctaca acactggagt catcaatgcc ccccagaagg tgatcgagga gttctacaac 1500 cagacatggg tccaccgcta tggggagagc atcctgccca ccacgctcac cacgctctgg 1560 tccctctcag tggccatctt ttctgttggg ggcatgattg gctccttctc tgtgggcctt 1620 ttcgttaacc gctttggccg gcggaattca atgctgatga tgaacctgct ggccttcgtg 1680 tccgccgtgc tcatgggctt ctcgaaactg ggcaagtcct ttgagatgct gatcctgggc 1740 cgcttcatca tcggtgtgta ctgtggcctg accacaggct tcgtgcccat gtatgtgggt 1800 gaagtgtcac ccacagccct tcgtggggcc ctgggcaccc tgcaccagct gggcatcgtc 1860 gtcggcatcc tcatcgccca ggtgttcggc ctggactcca tcatgggcaa caaggacctg 1920 tggcccctgc tgctgagcat catcttcatc ccggccctgc tgcagtgcat cgtgctgccc 1980 ttctgccccg agagtccccg cttcctgctc atcaaccgca acgaggagaa ccgggccaag 2040 agtgtgctaa agaagctgcg cgggacagct gacgtgaccc atgacctgca ggagatgaag 2100 gaagagagtc ggcagatgat gcgggagaag aaggtcacca tcctggagct gttccgctcc 2160 cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca gcagctgtct 2220 ggcatcaacg ctgtcttcta ttactccacg agcatcttcg agaaggcggg ggtgcagcag 2280 cctgtgtatg ccaccattgg ctccggtatc gtcaacacgg ccttcactgt cgtgtcgctg 2340 tttgtggtgg agcgagcagg ccggcggacc ctgcacctca taggcctcgc tggcatggcg 2400 ggttgtgcca tactcatgac catcgcgcta gcactgctgg agcagctacc ccggatgtcc 2460 tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtggg tcctggcccc 2520 atcccatggt tcatcgtggc tgaactcttc agccagggtc cacgtccagc tgccattgcc 2580 gttgcaggct tctccaactg gacctcaaat ttcattgtgg gcatgtgctt ccagtatgtg 2640 gagcaactgt gtggtcccta cgtcttcatc atcttcactg tgctcctggt tctgttcttc 2700 atcttcacct acttcaaagt tcctgagact aaaggccgga ccttcgatga gatcgcttcc 2760 ggcttccggc aggggggagc cagccaaagt gacaagacac ccgaggagct gttccatccc 2820 ctgggggctg attcccaagt gaccggtaat tttgaccttc ttaagcttgc gggagacgtc 2880 gagtccaacc ctgggcccgc catggtgagc aagggcgagg agctgttcac cggggtggtg 2940 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 3000 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 3060 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 3120 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 3180 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 3240 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 3300 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 3360 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 3420 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 3480 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 3540 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 3600 atggacgagc tgtacaagta aagcggccat caagcttatc ggccgttact agtggatcga 3660 ggacggggtg aactacgcct gaggatccga tctttttccc tctgccaaaa attatgggga 3720 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc 3780 aatagtgtgt tggaattttt tgtgtctctc actcggaagc aattcgttga tctgaatttc 3840 gaccacccat aatacccatt accctggtag ataagtagca tggcgggtta atcattaact 3900 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 3960 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 4020 agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact 4080 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 4140 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 4200 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 4260 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 4320 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 4380 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4440 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4500 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4560 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 4620 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 4680 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 4740 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 4800 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 4860 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 4920 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 4980 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 5040 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 5100 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 5160 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 5220 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 5280 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 5340 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 5400 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 5460 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 5520 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 5580 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 5640 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 5700 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 5760 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 5820 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 5880 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 5940 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 6000 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 6060 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 6120 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 6180 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 6240 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 6300 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 6360 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 6420 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 6480 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 6540 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 6600 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 6660 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 6720 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 6780 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta 6840 aggc 6844 <210> 20 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 20 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 21 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 21 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 22 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 22 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 23 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 <400> 23 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtga 1479 <210> 24 <211> 51 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: oligonucleótido sintético <220> <223> 2A-enlazador <400> 24 aattttgacc ttcttaagct tgcgggagac gtcgagtcca accctgggcc c 51 <210> 25 <211> 717 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> eGFP <400> 25 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 26 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 26 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 27 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 27 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 28 <211> 6568 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI hGlut1 <400> 28 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattggcc gccagtgtga 1200 tggatatctg cagaattcgc ccttagcagg agaccaaacg acgggggtcg gagtcagagt 1260 cgcagtggga gtccccggac cggagcacga gcctgagcgg gagagcgccg ctcgcacgcc 1320 cgtcgccacc cgcgtacccg gcgcagccag agccaccagc gcagcgctgc catggagccc 1380 agcagcaaga agctgacggg tcgcctcatg ctggccgtgg gaggagcagt gcttggctcc 1440 ctgcagtttg gctacaacac tggagtcatc aatgcccccc agaaggtgat cgaggagttc 1500 tacaaccaga catgggtcca ccgctatggg gagagcatcc tgcccaccac gctcaccacg 1560 ctctggtccc tctcagtggc catcttttct gttgggggca tgattggctc cttctctgtg 1620 ggccttttcg ttaaccgctt tggccggcgg aattcaatgc tgatgatgaa cctgctggcc 1680 ttcgtgtccg ccgtgctcat gggcttctcg aaactgggca agtcctttga gatgctgatc 1740 ctgggccgct tcatcatcgg tgtgtactgt ggcctgacca caggcttcgt gcccatgtat 1800 gtgggtgaag tgtcacccac agcccttcgt ggggccctgg gcaccctgca ccagctgggc 1860 atcgtcgtcg gcatcctcat cgcccaggtg ttcggcctgg actccatcat gggcaacaag 1920 gacctgtggc ccctgctgct gagcatcatc ttcatcccgg ccctgctgca gtgcatcgtg 1980 ctgcccttct gccccgagag tccccgcttc ctgctcatca accgcaacga ggagaaccgg 2040 gccaagagtg tgctaaagaa gctgcgcggg acagctgacg tgacccatga cctgcaggag 2100 atgaaggaag agagtcggca gatgatgcgg gagaagaagg tcaccatcct ggagctgttc 2160 cgctcccccg cctaccgcca gcccatcctc atcgctgtgg tgctgcagct gtcccagcag 2220 ctgtctggca tcaacgctgt cttctattac tccacgagca tcttcgagaa ggcgggggtg 2280 cagcagcctg tgtatgccac cattggctcc ggtatcgtca acacggcctt cactgtcgtg 2340 tcgctgtttg tggtggagcg agcaggccgg cggaccctgc acctcatagg cctcgctggc 2400 atggcgggtt gtgccatact catgaccatc gcgctagcac tgctggagca gctaccccgg 2460 atgtcctatc tgagcatcgt ggccatcttt ggctttgtgg ccttctttga agtgggtcct 2520 ggccccatcc catggttcat cgtggctgaa ctcttcagcc agggtccacg tccagctgcc 2580 attgccgttg caggcttctc caactggacc tcaaatttca ttgtgggcat gtgcttccag 2640 tatgtggagc aactgtgtgg tccctacgtc ttcatcatct tcactgtgct cctggttctg 2700 ttcttcatct tcacctactt caaagttcct gagactaaag gccggacctt cgatgagatc 2760 gcttccggct tccggcaggg gggagccagc caaagtgaca agacacccga ggagctgttc 2820 catcccctgg gggctgattc ccaagtgtga gtcgccccag atcaccagcc cggcctgctc 2880 ccagcagccc taaggatctc tcaggagcac aggcagctgg atgagacttc caaacctgac 2940 agatgtcagc cgagccgggc ctggggctcc tttctccagc cagcaatgat gtccagaaga 3000 atattcagga cttaacggct ccaggatttt aacaaaagca agactgttgc tcaaatctat 3060 tcagacaagc aacaggtttt ataatttttt tattactgat tttgttattt ttatatcagc 3120 ctgagtctcc tgtgcccaca tcccaggctt caccctgaat ggttccatgc ctgagggtgg 3180 agactaagcc ctgtcgagac acttgccttc ttcacccagc taatctgtag ggctggacct 3240 atgtcctaag gacacactaa tcgaactatg aactacaaag cttctatccc aggaggtggc 3300 tatggccacc cgttctgctg gcctggatct ccaagaaaca aagggcgaat tccagcacac 3360 tggcggccgt tactagtgga tcgaggacgg ggtgaactac gcctgaggat ccgatctttt 3420 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 3480 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 3540 aagcaattcg ttgatctgaa tttcgaccac ccataatacc cattaccctg gtagataagt 3600 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct 3660 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct 3720 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg 3780 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 3840 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 3900 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 3960 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 4020 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 4080 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 4140 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 4200 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 4260 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 4320 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 4380 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 4440 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 4500 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 4560 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 4620 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 4680 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 4740 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 4800 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 4860 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 4920 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 4980 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 5040 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 5100 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 5160 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 5220 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 5280 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 5340 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 5400 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 5460 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 5520 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 5580 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 5640 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 5700 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 5760 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 5820 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 5880 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 5940 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 6000 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 6060 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 6120 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 6180 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 6240 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 6300 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 6360 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 6420 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 6480 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 6540 ccatgattac gccagattta attaaggc 6568 <210> 29 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 29 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 30 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 30 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 31 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 31 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 32 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 <400> 32 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtga 1479 <210> 33 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 33 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 34 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 34 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 35 <211> 7057 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI mGlut1 <400> 35 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 tcccagcagc aagaaggtga cgggccgcct catgttggct gtgggaggag cagtgctcgg 1440 atcactgcag ttcggctata acactggtgt catcaacgcc ccccagaagg ttattgagga 1500 gttctacaat caaacatgga accaccgcta cggagagccc atcccatcca ccacactcac 1560 cacgctttgg tctctctccg tggccatctt ctctgtcggg ggcatgattg gttccttctc 1620 tgtcggcctc tttgttaatc gctttggcag gcggaactcc atgctgatga tgaacctgtt 1680 ggcctttgtg gctgctgtgc ttatgggctt ctccaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg actactggct ttgtgcccat 1800 gtatgtggga gaggtgtcac ctacagctct acgtggagcc ctaggcacac tgcaccagct 1860 gggaatcgtc gttggcatcc ttattgccca ggtgtttggc ttagactcca tcatgggcaa 1920 tgcagacttg tggcctctgc tgctcagtgt catcttcatc ccagccctgc tacagtgtat 1980 cctgttgccc ttctgccccg agagcccccg cttcctgctc atcaatcgta acgaggagaa 2040 ccgggccaag agtgtgctga agaagcttcg agggacagcc gatgtgaccc gagacctgca 2100 ggagatgaaa gaagagggtc ggcagatgat gcgggagaag aaggtcacca tcttggagct 2160 gttccgctca cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtcg ggtatcaatg ctgtgttcta ctactcaacg agcatcttcg agaaggcagg 2280 tgtgcagcag cctgtgtacg ccaccatcgg ctccggtatc gtcaacacgg ccttcactgt 2340 ggtgtcgctg tttgttgtag agcgagctgg acgacggacc ctgcacctca ttggcctggc 2400 tggcatggca ggctgtgctg tgctcatgac catcgccctg gccttgctgg aacggctgcc 2460 ttggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtagg 2520 ccctggtcct attccatggt tcattgtggc cgagctgttc agccaggggc cccgtcctgc 2580 tgctattgct gtggctggct tctccaactg gacctcaaac ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gcggccccta cgtcttcatc atcttcacgg tgctcctcgt 2700 gctcttcttc atcttcacct acttcaaagt ccctgagacc aaaggccgaa ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggtgc cagccaaagt gacaagacac ccgaggagct 2820 cttccaccct ctgggggcgg actcccaagt gtgaggagcc ccacacccag cccggcctgc 2880 tccctgcagc ccaaggatct ctctggagca caggcagcta gatgagacct cttccgaacc 2940 gacagatctc gggcaagccg ggcctgggcg cctttcctca gccagcagtg aagtccagga 3000 ggatattcag gactttgatg gctccagaat ttttaatgaa agcaagactg ctgctcagat 3060 ctattcagat aagcagcagg ttttataatt tttttattac tgattttgtt attttttttt 3120 tttatcagcc actctcctat ctccacactg tagtcttcac cttgattggc ccagtgcctg 3180 agggtgggga ccacgccctg tccagacact tgccttcttt gccaagctaa tctgtagggc 3240 tggacctatg gccaaggaca cactaatacc gaactctgag ctaggaggct ttaccgctgg 3300 aggcggtagc tgccacccac ttccgcaggc ctggacctcg gcaccatagg ggtccggact 3360 ccattttagg attcgcccat tcctgtctct tcctacccaa ccactcaatt aatctttcct 3420 tgcctgagac cagttggaag cactggagtg cagggaggag agggaagggc caggctgggc 3480 tgccaggttc tagtctcctg tgcactgagg gccacacaaa caccatgaga aggacctcgg 3540 aggctgagaa cttaactgct gaagacacgg acactcctgc cctgctgtgt atagatggaa 3600 gatatttata tattttttgg ttgtcaatat taaatacaga cactaagtta tagtatatct 3660 ggacaaaccc acttgtaaat acaccaacaa actcctgtaa ctttacctaa gcagatataa 3720 atggctggtt tttagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3780 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actgtctcgc tgcggccgct ctagagtatc 3840 cctcgactct agagtcgacc cgggcggcct cgaggacggg gtgaactacg cctgaggatc 3900 cgatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 3960 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 4020 ctcactcgga agcaattcgt tgatctgaat ttcgaccacc cataataccc attaccctgg 4080 tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg 4140 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 4200 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta 4260 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 4320 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 4380 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 4440 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 4500 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 4560 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 4620 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 4680 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 4740 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 4800 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 4860 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4920 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4980 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 5040 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 5100 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 5160 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 5220 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 5280 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 5340 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 5400 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 5460 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 5520 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 5580 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 5640 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 5700 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 5760 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 5820 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 5880 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5940 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 6000 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 6060 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 6120 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 6180 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 6240 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 6300 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 6360 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 6420 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 6480 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 6540 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6600 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 6660 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 6720 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 6780 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 6840 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 6900 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 6960 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 7020 cagctatgac catgattacg ccagatttaa ttaaggc 7057 <210> 36 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 36 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 37 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 37 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 38 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 38 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 39 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de mGlut1 <400> 39 atggatccca gcagcaagaa ggtgacgggc cgcctcatgt tggctgtggg aggagcagtg 60 ctcggatcac tgcagttcgg ctataacact ggtgtcatca acgcccccca gaaggttatt 120 gaggagttct acaatcaaac atggaaccac cgctacggag agcccatccc atccaccaca 180 ctcaccacgc tttggtctct ctccgtggcc atcttctctg tcgggggcat gattggttcc 240 ttctctgtcg gcctctttgt taatcgcttt ggcaggcgga actccatgct gatgatgaac 300 ctgttggcct ttgtggctgc tgtgcttatg ggcttctcca aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgcg gcctgactac tggctttgtg 420 cccatgtatg tgggagaggt gtcacctaca gctctacgtg gagccctagg cacactgcac 480 cagctgggaa tcgtcgttgg catccttatt gcccaggtgt ttggcttaga ctccatcatg 540 ggcaatgcag acttgtggcc tctgctgctc agtgtcatct tcatcccagc cctgctacag 600 tgtatcctgt tgcccttctg ccccgagagc ccccgcttcc tgctcatcaa tcgtaacgag 660 gagaaccggg ccaagagtgt gctgaagaag cttcgaggga cagccgatgt gacccgagac 720 ctgcaggaga tgaaagaaga gggtcggcag atgatgcggg agaagaaggt caccatcttg 780 gagctgttcc gctcacccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtcgggtat caatgctgtg ttctactact caacgagcat cttcgagaag 900 gcaggtgtgc agcagcctgt gtacgccacc atcggctccg gtatcgtcaa cacggccttc 960 actgtggtgt cgctgtttgt tgtagagcga gctggacgac ggaccctgca cctcattggc 1020 ctggctggca tggcaggctg tgctgtgctc atgaccatcg ccctggcctt gctggaacgg 1080 ctgccttgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtaggccctg gtcctattcc atggttcatt gtggccgagc tgttcagcca ggggccccgt 1200 cctgctgcta ttgctgtggc tggcttctcc aactggacct caaacttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgcggc ccctacgtct tcatcatctt cacggtgctc 1320 ctcgtgctct tcttcatctt cacctacttc aaagtccctg agaccaaagg ccgaaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggtgccagcc aaagtgacaa gacacccgag 1440 gagctcttcc accctctggg ggcggactcc caagtgtga 1479 <210> 40 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 40 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 41 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 41 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 42 <211> 6641 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI hGlut1-out3xmiR-122 BS <400> 42 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcagctc ttaaggctag 1140 agtacttaat acgactcact ataggctagc gcgccgaatt ggccgccagt gtgatggata 1200 tctgcagaat tcgcccttag caggagacca aacgacgggg gtcggagtca gagtcgcagt 1260 gggagtcccc ggaccggagc acgagcctga gcgggagagc gccgctcgca cgcccgtcgc 1320 cacccgcgta cccggcgcag ccagagccac cagcgcagcg ctgccatgga gcccagcagc 1380 aagaagctga cgggtcgcct catgctggcc gtgggaggag cagtgcttgg ctccctgcag 1440 tttggctaca acactggagt catcaatgcc ccccagaagg tgatcgagga gttctacaac 1500 cagacatggg tccaccgcta tggggagagc atcctgccca ccacgctcac cacgctctgg 1560 tccctctcag tggccatctt ttctgttggg ggcatgattg gctccttctc tgtgggcctt 1620 ttcgttaacc gctttggccg gcggaattca atgctgatga tgaacctgct ggccttcgtg 1680 tccgccgtgc tcatgggctt ctcgaaactg ggcaagtcct ttgagatgct gatcctgggc 1740 cgcttcatca tcggtgtgta ctgtggcctg accacaggct tcgtgcccat gtatgtgggt 1800 gaagtgtcac ccacagccct tcgtggggcc ctgggcaccc tgcaccagct gggcatcgtc 1860 gtcggcatcc tcatcgccca ggtgttcggc ctggactcca tcatgggcaa caaggacctg 1920 tggcccctgc tgctgagcat catcttcatc ccggccctgc tgcagtgcat cgtgctgccc 1980 ttctgccccg agagtccccg cttcctgctc atcaaccgca acgaggagaa ccgggccaag 2040 agtgtgctaa agaagctgcg cgggacagct gacgtgaccc atgacctgca ggagatgaag 2100 gaagagagtc ggcagatgat gcgggagaag aaggtcacca tcctggagct gttccgctcc 2160 cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca gcagctgtct 2220 ggcatcaacg ctgtcttcta ttactccacg agcatcttcg agaaggcggg ggtgcagcag 2280 cctgtgtatg ccaccattgg ctccggtatc gtcaacacgg ccttcactgt cgtgtcgctg 2340 tttgtggtgg agcgagcagg ccggcggacc ctgcacctca taggcctcgc tggcatggcg 2400 ggttgtgcca tactcatgac catcgcgcta gcactgctgg agcagctacc ccggatgtcc 2460 tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtggg tcctggcccc 2520 atcccatggt tcatcgtggc tgaactcttc agccagggtc cacgtccagc tgccattgcc 2580 gttgcaggct tctccaactg gacctcaaat ttcattgtgg gcatgtgctt ccagtatgtg 2640 gagcaactgt gtggtcccta cgtcttcatc atcttcactg tgctcctggt tctgttcttc 2700 atcttcacct acttcaaagt tcctgagact aaaggccgga ccttcgatga gatcgcttcc 2760 ggcttccggc aggggggagc cagccaaagt gacaagacac ccgaggagct gttccatccc 2820 ctgggggctg attcccaagt gtgagtcgcc ccagatcacc agcccggcct gctcccagca 2880 gccctaagga tctctcagga gcacaggcag ctggatgaga cttccaaacc tgacagatgt 2940 cagccgagcc gggcctgggg ctcctttctc cagccagcaa tgatgtccag aagaatattc 3000 aggacttaac ggctccagga ttttaacaaa agcaagactg ttgctcaaat ctattcagac 3060 aagcaacagg ttttataatt tttttattac tgattttgtt atttttatat cagcctgagt 3120 ctcctgtgcc cacatcccag gcttcaccct gaatggttcc atgcctgagg gtggagacta 3180 agccctgtcg agacacttgc cttcttcacc cagctaatct gtagggctgg acctatgtcc 3240 taaggacaca ctaatcgaac tatgaactac aaagcttcta tcccaggagg tggctatggc 3300 cacccgttct gctggcctgg atctcccaag aaacaaaggg cgaattccag cacactggcg 3360 gcccgaaaca aacaccattg tcacactcca acaaacacca ttgtcacact ccaacaaaca 3420 ccattgtcac actccattcg ggttactagt ggatcgagga cggggtgaac tacgcctgag 3480 gatccgatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc 3540 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt 3600 gtctctcact cggaagcaat tcgttgatct gaatttcgac cacccataat acccattacc 3660 ctggtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag 3720 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 3780 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa 3840 cctaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 3900 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc 3960 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc 4020 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 4080 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 4140 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4200 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 4260 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4320 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 4380 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 4440 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta 4500 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4560 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4620 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4680 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4740 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4800 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4860 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4920 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 4980 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 5040 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 5100 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 5160 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 5220 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 5280 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 5340 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 5400 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 5460 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5520 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5580 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5640 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5700 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5760 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5820 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5880 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5940 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6000 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6060 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6120 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6180 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6240 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6300 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 6360 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 6420 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 6480 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 6540 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag 6600 gaaacagcta tgaccatgat tacgccagat ttaattaagg c 6641 <210> 43 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 43 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 44 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 44 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 45 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 45 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 46 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 <400> 46 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtga 1479 <210> 47 <211> 482 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3'UTR <400> 47 gtcgccccag atcaccagcc cggcctgctc ccagcagccc taaggatctc tcaggagcac 60 aggcagctgg atgagacttc caaacctgac agatgtcagc cgagccgggc ctggggctcc 120 tttctccagc cagcaatgat gtccagaaga atattcagga cttaacggct ccaggatttt 180 aacaaaagca agactgttgc tcaaatctat tcagacaagc aacaggtttt ataatttttt 240 tattactgat tttgttattt ttatatcagc ctgagtctcc tgtgcccaca tcccaggctt 300 caccctgaat ggttccatgc ctgagggtgg agactaagcc ctgtcgagac acttgccttc 360 ttcacccagc taatctgtag ggctggacct atgtcctaag gacacactaa tcgaactatg 420 aactacaaag cttctatccc aggaggtggc tatggccacc cgttctgctg gcctggatct 480 cc 482 <210> 48 <211> 77 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: oligonucleótido sintético <220> <223> 3xmiR-122 BS <400> 48 cgaaacaaac accattgtca cactccaaca aacaccattg tcacactcca acaaacacca 60 ttgtcacact ccattcg 77 <210> 49 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 49 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 50 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 50 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 51 <211> 7137 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI hGlut1-in3xmiR-122 BS <400> 51 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 gcccagcagc aagaagctga cgggtcgcct catgctggcc gtgggaggag cagtgcttgg 1440 ctccctgcag tttggctaca acactggagt catcaatgcc ccccagaagg tgatcgagga 1500 gttctacaac cagacatggg tccaccgcta tggggagagc atcctgccca ccacgctcac 1560 cacgctctgg tccctctcag tggccatctt ttctgttggg ggcatgattg gctccttctc 1620 tgtgggcctt ttcgttaacc gctttggccg gcggaattca atgctgatga tgaacctgct 1680 ggccttcgtg tccgccgtgc tcatgggctt ctcgaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgtggcctg accacaggct tcgtgcccat 1800 gtatgtgggt gaagtgtcac ccacagccct tcgtggggcc ctgggcaccc tgcaccagct 1860 gggcatcgtc gtcggcatcc tcatcgccca ggtgttcggc ctggactcca tcatgggcaa 1920 caaggacctg tggcccctgc tgctgagcat catcttcatc ccggccctgc tgcagtgcat 1980 cgtgctgccc ttctgccccg agagtccccg cttcctgctc atcaaccgca acgaggagaa 2040 ccgggccaag agtgtgctaa agaagctgcg cgggacagct gacgtgaccc atgacctgca 2100 ggagatgaag gaagagagtc ggcagatgat gcgggagaag aaggtcacca tcctggagct 2160 gttccgctcc cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtct ggcatcaacg ctgtcttcta ttactccacg agcatcttcg agaaggcggg 2280 ggtgcagcag cctgtgtatg ccaccattgg ctccggtatc gtcaacacgg ccttcactgt 2340 cgtgtcgctg tttgtggtgg agcgagcagg ccggcggacc ctgcacctca taggcctcgc 2400 tggcatggcg ggttgtgcca tactcatgac catcgcgcta gcactgctgg agcagctacc 2460 ccggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtggg 2520 tcctggcccc atcccatggt tcatcgtggc tgaactcttc agccagggtc cacgtccagc 2580 tgccattgcc gttgcaggct tctccaactg gacctcaaat ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gtggtcccta cgtcttcatc atcttcactg tgctcctggt 2700 tctgttcttc atcttcacct acttcaaagt tcctgagact aaaggccgga ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggagc cagccaaagt gacaagacac ccgaggagct 2820 gttccatccc ctgggggctg attcccaagt gtgaggagcc ccacacccag cccggcctgc 2880 tccctgcagc ccaaggatct ctctggagca caggcagcta gatgagacct cttccgaacc 2940 gacagatctc gggcaagccg ggcctgggcg cctttcctca gccagcagtg aagtccagga 3000 ggatattcag gactttgatg gctccagaat ttttaatgaa agcaagactg ctgctcagat 3060 ctattcagat aagcagcagg ttttataatt tttttattac tgattttgtt attttttttt 3120 tttatcagcc actctcctat ctccacactg tagtcttcac cttgattggc ccagtgcctg 3180 agggtgggga ccacgccctg tccagacact tgccttcttt gccaagctaa tctgtagggc 3240 tggacctatg gccaaggaca cactaatacc gaactctgag ctaggaggct ttaccgctgg 3300 aggcggtagc tgccacccac ttccgcaggc ctggacctcg gcaccatagg ggtccggact 3360 ccattttagg attcgcccat tcctgtctct tcctacccaa ccactcaatt aatctttcct 3420 tgcctgagac cagttggaag cactggagtg cagggaggag agggaagggc caggctgggc 3480 tgccaggttc tagtctcctg tgcactgagg gccacacaaa caccatgaga aggaccgaaa 3540 caaacaccat tgtcacactc caacaaacac cattgtcaca ctccaacaaa caccattgtc 3600 acactccatt cggacctcgg aggctgagaa cttaactgct gaagacacgg acactcctgc 3660 cctgctgtgt atagatggaa gatatttata tattttttgg ttgtcaatat taaatacaga 3720 cactaagtta tagtatatct ggacaaaccc acttgtaaat acaccaacaa actcctgtaa 3780 ctttacctaa gcagatataa atggctggtt tttagaaaaa aaaaaaaaaa aaaaaaaaaa 3840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actgtctcgc 3900 tgcggccgct ctagagtatc cctcgactct agagtcgacc cgggcggcct cgaggacggg 3960 gtgaactacg cctgaggatc cgatcttttt ccctctgcca aaaattatgg ggacatcatg 4020 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 4080 tgttggaatt ttttgtgtct ctcactcgga agcaattcgt tgatctgaat ttcgaccacc 4140 cataataccc attaccctgg tagataagta gcatggcggg ttaatcatta actacaagga 4200 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 4260 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 4320 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa 4380 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 4440 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 4500 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 4560 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 4620 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 4680 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 4740 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 4800 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 4860 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 4920 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 4980 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 5040 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 5100 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 5160 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 5220 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 5280 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 5340 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 5400 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 5460 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 5520 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 5580 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 5640 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 5700 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 5760 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 5820 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 5880 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 5940 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 6000 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 6060 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 6120 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 6180 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 6240 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 6300 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 6360 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6420 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6480 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6540 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6600 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6660 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6720 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 6780 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 6840 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 6900 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 6960 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 7020 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 7080 taacaatttc acacaggaaa cagctatgac catgattacg ccagatttaa ttaaggc 7137 <210> 52 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 52 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 53 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 53 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 54 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 54 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 55 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 <400> 55 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtga 1479 <210> 56 <211> 1035 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' UTR y 3xmiR-122 <400> 56 ggagccccac acccagcccg gcctgctccc tgcagcccaa ggatctctct ggagcacagg 60 cagctagatg agacctcttc cgaaccgaca gatctcgggc aagccgggcc tgggcgcctt 120 tcctcagcca gcagtgaagt ccaggaggat attcaggact ttgatggctc cagaattttt 180 aatgaaagca agactgctgc tcagatctat tcagataagc agcaggtttt ataatttttt 240 tattactgat tttgttattt ttttttttta tcagccactc tcctatctcc acactgtagt 300 cttcaccttg attggcccag tgcctgaggg tggggaccac gccctgtcca gacacttgcc 360 ttctttgcca agctaatctg tagggctgga cctatggcca aggacacact aataccgaac 420 tctgagctag gaggctttac cgctggaggc ggtagctgcc acccacttcc gcaggcctgg 480 acctcggcac cataggggtc cggactccat tttaggattc gcccattcct gtctcttcct 540 acccaaccac tcaattaatc tttccttgcc tgagaccagt tggaagcact ggagtgcagg 600 gaggagaggg aagggccagg ctgggctgcc aggttctagt ctcctgtgca ctgagggcca 660 cacaaacacc atgagaagga ccgaaacaaa caccattgtc acactccaac aaacaccatt 720 gtcacactcc aacaaacacc attgtcacac tccattcgga cctcggaggc tgagaactta 780 actgctgaag acacggacac tcctgccctg ctgtgtatag atggaagata tttatatatt 840 ttttggttgt caatattaaa tacagacact aagttatagt atatctggac aaacccactt 900 gtaaatacac caacaaactc ctgtaacttt acctaagcag atataaatgg ctggttttta 960 gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1020 aaaaaaaaaa aaaaa 1035 <210> 57 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 57 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 58 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 58 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 59 <211> 7137 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI mGlut1-in3xmiR-122 BS <400> 59 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 tcccagcagc aagaaggtga cgggccgcct catgttggct gtgggaggag cagtgctcgg 1440 atcactgcag ttcggctata acactggtgt catcaacgcc ccccagaagg ttattgagga 1500 gttctacaat caaacatgga accaccgcta cggagagccc atcccatcca ccacactcac 1560 cacgctttgg tctctctccg tggccatctt ctctgtcggg ggcatgattg gttccttctc 1620 tgtcggcctc tttgttaatc gctttggcag gcggaactcc atgctgatga tgaacctgtt 1680 ggcctttgtg gctgctgtgc ttatgggctt ctccaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg actactggct ttgtgcccat 1800 gtatgtggga gaggtgtcac ctacagctct acgtggagcc ctaggcacac tgcaccagct 1860 gggaatcgtc gttggcatcc ttattgccca ggtgtttggc ttagactcca tcatgggcaa 1920 tgcagacttg tggcctctgc tgctcagtgt catcttcatc ccagccctgc tacagtgtat 1980 cctgttgccc ttctgccccg agagcccccg cttcctgctc atcaatcgta acgaggagaa 2040 ccgggccaag agtgtgctga agaagcttcg agggacagcc gatgtgaccc gagacctgca 2100 ggagatgaaa gaagagggtc ggcagatgat gcgggagaag aaggtcacca tcttggagct 2160 gttccgctca cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtcg ggtatcaatg ctgtgttcta ctactcaacg agcatcttcg agaaggcagg 2280 tgtgcagcag cctgtgtacg ccaccatcgg ctccggtatc gtcaacacgg ccttcactgt 2340 ggtgtcgctg tttgttgtag agcgagctgg acgacggacc ctgcacctca ttggcctggc 2400 tggcatggca ggctgtgctg tgctcatgac catcgccctg gccttgctgg aacggctgcc 2460 ttggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtagg 2520 ccctggtcct attccatggt tcattgtggc cgagctgttc agccaggggc cccgtcctgc 2580 tgctattgct gtggctggct tctccaactg gacctcaaac ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gcggccccta cgtcttcatc atcttcacgg tgctcctcgt 2700 gctcttcttc atcttcacct acttcaaagt ccctgagacc aaaggccgaa ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggtgc cagccaaagt gacaagacac ccgaggagct 2820 cttccaccct ctgggggcgg actcccaagt gtgaggagcc ccacacccag cccggcctgc 2880 tccctgcagc ccaaggatct ctctggagca caggcagcta gatgagacct cttccgaacc 2940 gacagatctc gggcaagccg ggcctgggcg cctttcctca gccagcagtg aagtccagga 3000 ggatattcag gactttgatg gctccagaat ttttaatgaa agcaagactg ctgctcagat 3060 ctattcagat aagcagcagg ttttataatt tttttattac tgattttgtt attttttttt 3120 tttatcagcc actctcctat ctccacactg tagtcttcac cttgattggc ccagtgcctg 3180 agggtgggga ccacgccctg tccagacact tgccttcttt gccaagctaa tctgtagggc 3240 tggacctatg gccaaggaca cactaatacc gaactctgag ctaggaggct ttaccgctgg 3300 aggcggtagc tgccacccac ttccgcaggc ctggacctcg gcaccatagg ggtccggact 3360 ccattttagg attcgcccat tcctgtctct tcctacccaa ccactcaatt aatctttcct 3420 tgcctgagac cagttggaag cactggagtg cagggaggag agggaagggc caggctgggc 3480 tgccaggttc tagtctcctg tgcactgagg gccacacaaa caccatgaga aggaccgaaa 3540 caaacaccat tgtcacactc caacaaacac cattgtcaca ctccaacaaa caccattgtc 3600 acactccatt cggacctcgg aggctgagaa cttaactgct gaagacacgg acactcctgc 3660 cctgctgtgt atagatggaa gatatttata tattttttgg ttgtcaatat taaatacaga 3720 cactaagtta tagtatatct ggacaaaccc acttgtaaat acaccaacaa actcctgtaa 3780 ctttacctaa gcagatataa atggctggtt tttagaaaaa aaaaaaaaaa aaaaaaaaaa 3840 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actgtctcgc 3900 tgcggccgct ctagagtatc cctcgactct agagtcgacc cgggcggcct cgaggacggg 3960 gtgaactacg cctgaggatc cgatcttttt ccctctgcca aaaattatgg ggacatcatg 4020 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg 4080 tgttggaatt ttttgtgtct ctcactcgga agcaattcgt tgatctgaat ttcgaccacc 4140 cataataccc attaccctgg tagataagta gcatggcggg ttaatcatta actacaagga 4200 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg 4260 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc 4320 gcgcagcctt aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa 4380 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 4440 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 4500 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 4560 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 4620 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 4680 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 4740 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 4800 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 4860 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 4920 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat 4980 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 5040 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 5100 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 5160 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 5220 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 5280 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 5340 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 5400 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 5460 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 5520 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 5580 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 5640 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 5700 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 5760 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 5820 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 5880 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 5940 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 6000 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 6060 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 6120 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 6180 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 6240 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc 6300 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 6360 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6420 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6480 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6540 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6600 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6660 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6720 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 6780 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 6840 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 6900 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 6960 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 7020 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 7080 taacaatttc acacaggaaa cagctatgac catgattacg ccagatttaa ttaaggc 7137 <210> 60 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 60 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 61 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 61 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 62 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 62 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 63 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de mGlut1 <400> 63 atggatccca gcagcaagaa ggtgacgggc cgcctcatgt tggctgtggg aggagcagtg 60 ctcggatcac tgcagttcgg ctataacact ggtgtcatca acgcccccca gaaggttatt 120 gaggagttct acaatcaaac atggaaccac cgctacggag agcccatccc atccaccaca 180 ctcaccacgc tttggtctct ctccgtggcc atcttctctg tcgggggcat gattggttcc 240 ttctctgtcg gcctctttgt taatcgcttt ggcaggcgga actccatgct gatgatgaac 300 ctgttggcct ttgtggctgc tgtgcttatg ggcttctcca aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgcg gcctgactac tggctttgtg 420 cccatgtatg tgggagaggt gtcacctaca gctctacgtg gagccctagg cacactgcac 480 cagctgggaa tcgtcgttgg catccttatt gcccaggtgt ttggcttaga ctccatcatg 540 ggcaatgcag acttgtggcc tctgctgctc agtgtcatct tcatcccagc cctgctacag 600 tgtatcctgt tgcccttctg ccccgagagc ccccgcttcc tgctcatcaa tcgtaacgag 660 gagaaccggg ccaagagtgt gctgaagaag cttcgaggga cagccgatgt gacccgagac 720 ctgcaggaga tgaaagaaga gggtcggcag atgatgcggg agaagaaggt caccatcttg 780 gagctgttcc gctcacccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtcgggtat caatgctgtg ttctactact caacgagcat cttcgagaag 900 gcaggtgtgc agcagcctgt gtacgccacc atcggctccg gtatcgtcaa cacggccttc 960 actgtggtgt cgctgtttgt tgtagagcga gctggacgac ggaccctgca cctcattggc 1020 ctggctggca tggcaggctg tgctgtgctc atgaccatcg ccctggcctt gctggaacgg 1080 ctgccttgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtaggccctg gtcctattcc atggttcatt gtggccgagc tgttcagcca ggggccccgt 1200 cctgctgcta ttgctgtggc tggcttctcc aactggacct caaacttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgcggc ccctacgtct tcatcatctt cacggtgctc 1320 ctcgtgctct tcttcatctt cacctacttc aaagtccctg agaccaaagg ccgaaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggtgccagcc aaagtgacaa gacacccgag 1440 gagctcttcc accctctggg ggcggactcc caagtgtga 1479 <210> 64 <211> 1035 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3'UTR y 3x-miR122BS <400> 64 ggagccccac acccagcccg gcctgctccc tgcagcccaa ggatctctct ggagcacagg 60 cagctagatg agacctcttc cgaaccgaca gatctcgggc aagccgggcc tgggcgcctt 120 tcctcagcca gcagtgaagt ccaggaggat attcaggact ttgatggctc cagaattttt 180 aatgaaagca agactgctgc tcagatctat tcagataagc agcaggtttt ataatttttt 240 tattactgat tttgttattt ttttttttta tcagccactc tcctatctcc acactgtagt 300 cttcaccttg attggcccag tgcctgaggg tggggaccac gccctgtcca gacacttgcc 360 ttctttgcca agctaatctg tagggctgga cctatggcca aggacacact aataccgaac 420 tctgagctag gaggctttac cgctggaggc ggtagctgcc acccacttcc gcaggcctgg 480 acctcggcac cataggggtc cggactccat tttaggattc gcccattcct gtctcttcct 540 acccaaccac tcaattaatc tttccttgcc tgagaccagt tggaagcact ggagtgcagg 600 gaggagaggg aagggccagg ctgggctgcc aggttctagt ctcctgtgca ctgagggcca 660 cacaaacacc atgagaagga ccgaaacaaa caccattgtc acactccaac aaacaccatt 720 gtcacactcc aacaaacacc attgtcacac tccattcgga cctcggaggc tgagaactta 780 actgctgaag acacggacac tcctgccctg ctgtgtatag atggaagata tttatatatt 840 ttttggttgt caatattaaa tacagacact aagttatagt atatctggac aaacccactt 900 gtaaatacac caacaaactc ctgtaacttt acctaagcag atataaatgg ctggttttta 960 gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1020 aaaaaaaaaa aaaaa 1035 <210> 65 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 65 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 66 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3'ITR <400> 66 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 67 <211> 7138 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI mGlut1-out3xmiR-122 BS <400> 67 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 tcccagcagc aagaaggtga cgggccgcct catgttggct gtgggaggag cagtgctcgg 1440 atcactgcag ttcggctata acactggtgt catcaacgcc ccccagaagg ttattgagga 1500 gttctacaat caaacatgga accaccgcta cggagagccc atcccatcca ccacactcac 1560 cacgctttgg tctctctccg tggccatctt ctctgtcggg ggcatgattg gttccttctc 1620 tgtcggcctc tttgttaatc gctttggcag gcggaactcc atgctgatga tgaacctgtt 1680 ggcctttgtg gctgctgtgc ttatgggctt ctccaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg actactggct ttgtgcccat 1800 gtatgtggga gaggtgtcac ctacagctct acgtggagcc ctaggcacac tgcaccagct 1860 gggaatcgtc gttggcatcc ttattgccca ggtgtttggc ttagactcca tcatgggcaa 1920 tgcagacttg tggcctctgc tgctcagtgt catcttcatc ccagccctgc tacagtgtat 1980 cctgttgccc ttctgccccg agagcccccg cttcctgctc atcaatcgta acgaggagaa 2040 ccgggccaag agtgtgctga agaagcttcg agggacagcc gatgtgaccc gagacctgca 2100 ggagatgaaa gaagagggtc ggcagatgat gcgggagaag aaggtcacca tcttggagct 2160 gttccgctca cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtcg ggtatcaatg ctgtgttcta ctactcaacg agcatcttcg agaaggcagg 2280 tgtgcagcag cctgtgtacg ccaccatcgg ctccggtatc gtcaacacgg ccttcactgt 2340 ggtgtcgctg tttgttgtag agcgagctgg acgacggacc ctgcacctca ttggcctggc 2400 tggcatggca ggctgtgctg tgctcatgac catcgccctg gccttgctgg aacggctgcc 2460 ttggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtagg 2520 ccctggtcct attccatggt tcattgtggc cgagctgttc agccaggggc cccgtcctgc 2580 tgctattgct gtggctggct tctccaactg gacctcaaac ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gcggccccta cgtcttcatc atcttcacgg tgctcctcgt 2700 gctcttcttc atcttcacct acttcaaagt ccctgagacc aaaggccgaa ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggtgc cagccaaagt gacaagacac ccgaggagct 2820 cttccaccct ctgggggcgg actcccaagt gtgaggagcc ccacacccag cccggcctgc 2880 tccctgcagc ccaaggatct ctctggagca caggcagcta gatgagacct cttccgaacc 2940 gacagatctc gggcaagccg ggcctgggcg cctttcctca gccagcagtg aagtccagga 3000 ggatattcag gactttgatg gctccagaat ttttaatgaa agcaagactg ctgctcagat 3060 ctattcagat aagcagcagg ttttataatt tttttattac tgattttgtt attttttttt 3120 tttatcagcc actctcctat ctccacactg tagtcttcac cttgattggc ccagtgcctg 3180 agggtgggga ccacgccctg tccagacact tgccttcttt gccaagctaa tctgtagggc 3240 tggacctatg gccaaggaca cactaatacc gaactctgag ctaggaggct ttaccgctgg 3300 aggcggtagc tgccacccac ttccgcaggc ctggacctcg gcaccatagg ggtccggact 3360 ccattttagg attcgcccat tcctgtctct tcctacccaa ccactcaatt aatctttcct 3420 tgcctgagac cagttggaag cactggagtg cagggaggag agggaagggc caggctgggc 3480 tgccaggttc tagtctcctg tgcactgagg gccacacaaa caccatgaga aggacctcgg 3540 aggctgagaa cttaactgct gaagacacgg acactcctgc cctgctgtgt atagatggaa 3600 gatatttata tattttttgg ttgtcaatat taaatacaga cactaagtta tagtatatct 3660 ggacaaaccc acttgtaaat acaccaacaa actcctgtaa ctttacctaa gcagatataa 3720 atggctggtt tttagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3780 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actgtctcgc tgcggcccga aacaaacacc 3840 attgtcacac tccaacaaac accattgtca cactccaaca aacaccattg tcacactcca 3900 ttcgggccgc tctagagtat ccctcgactc tagagtcgac ccgggcggcc tcgaggacgg 3960 ggtgaactac gcctgaggat ccgatctttt tccctctgcc aaaaattatg gggacatcat 4020 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt 4080 gtgttggaat tttttgtgtc tctcactcgg aagcaattcg ttgatctgaa tttcgaccac 4140 ccataatacc cattaccctg gtagataagt agcatggcgg gttaatcatt aactacaagg 4200 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 4260 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 4320 cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 4380 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 4440 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 4500 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4560 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4620 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4680 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 4740 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 4800 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 4860 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 4920 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa 4980 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 5040 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 5100 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 5160 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 5220 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 5280 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 5340 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 5400 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 5460 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 5520 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 5580 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 5640 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 5700 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 5760 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 5820 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 5880 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 5940 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 6000 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 6060 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 6120 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 6180 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 6240 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 6300 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 6360 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 6420 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 6480 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 6540 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 6600 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 6660 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 6720 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 6780 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 6840 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 6900 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 6960 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 7020 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 7080 ataacaattt cacacaggaa acagctatga ccatgattac gccagattta attaaggc 7138 <210> 68 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 68 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 69 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 69 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 70 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 70 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 71 <211> 1479 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de mGlut1 <400> 71 atggatccca gcagcaagaa ggtgacgggc cgcctcatgt tggctgtggg aggagcagtg 60 ctcggatcac tgcagttcgg ctataacact ggtgtcatca acgcccccca gaaggttatt 120 gaggagttct acaatcaaac atggaaccac cgctacggag agcccatccc atccaccaca 180 ctcaccacgc tttggtctct ctccgtggcc atcttctctg tcgggggcat gattggttcc 240 ttctctgtcg gcctctttgt taatcgcttt ggcaggcgga actccatgct gatgatgaac 300 ctgttggcct ttgtggctgc tgtgcttatg ggcttctcca aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgcg gcctgactac tggctttgtg 420 cccatgtatg tgggagaggt gtcacctaca gctctacgtg gagccctagg cacactgcac 480 cagctgggaa tcgtcgttgg catccttatt gcccaggtgt ttggcttaga ctccatcatg 540 ggcaatgcag acttgtggcc tctgctgctc agtgtcatct tcatcccagc cctgctacag 600 tgtatcctgt tgcccttctg ccccgagagc ccccgcttcc tgctcatcaa tcgtaacgag 660 gagaaccggg ccaagagtgt gctgaagaag cttcgaggga cagccgatgt gacccgagac 720 ctgcaggaga tgaaagaaga gggtcggcag atgatgcggg agaagaaggt caccatcttg 780 gagctgttcc gctcacccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtcgggtat caatgctgtg ttctactact caacgagcat cttcgagaag 900 gcaggtgtgc agcagcctgt gtacgccacc atcggctccg gtatcgtcaa cacggccttc 960 actgtggtgt cgctgtttgt tgtagagcga gctggacgac ggaccctgca cctcattggc 1020 ctggctggca tggcaggctg tgctgtgctc atgaccatcg ccctggcctt gctggaacgg 1080 ctgccttgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtaggccctg gtcctattcc atggttcatt gtggccgagc tgttcagcca ggggccccgt 1200 cctgctgcta ttgctgtggc tggcttctcc aactggacct caaacttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgcggc ccctacgtct tcatcatctt cacggtgctc 1320 ctcgtgctct tcttcatctt cacctacttc aaagtccctg agaccaaagg ccgaaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggtgccagcc aaagtgacaa gacacccgag 1440 gagctcttcc accctctggg ggcggactcc caagtgtga 1479 <210> 72 <211> 955 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3'UTR <400> 72 ggagccccac acccagcccg gcctgctccc tgcagcccaa ggatctctct ggagcacagg 60 cagctagatg agacctcttc cgaaccgaca gatctcgggc aagccgggcc tgggcgcctt 120 tcctcagcca gcagtgaagt ccaggaggat attcaggact ttgatggctc cagaattttt 180 aatgaaagca agactgctgc tcagatctat tcagataagc agcaggtttt ataatttttt 240 tattactgat tttgttattt ttttttttta tcagccactc tcctatctcc acactgtagt 300 cttcaccttg attggcccag tgcctgaggg tggggaccac gccctgtcca gacacttgcc 360 ttctttgcca agctaatctg tagggctgga cctatggcca aggacacact aataccgaac 420 tctgagctag gaggctttac cgctggaggc ggtagctgcc acccacttcc gcaggcctgg 480 acctcggcac cataggggtc cggactccat tttaggattc gcccattcct gtctcttcct 540 acccaaccac tcaattaatc tttccttgcc tgagaccagt tggaagcact ggagtgcagg 600 gaggagaggg aagggccagg ctgggctgcc aggttctagt ctcctgtgca ctgagggcca 660 cacaaacacc atgagaagga cctcggaggc tgagaactta actgctgaag acacggacac 720 tcctgccctg ctgtgtatag atggaagata tttatatatt ttttggttgt caatattaaa 780 tacagacact aagttatagt atatctggac aaacccactt gtaaatacac caacaaactc 840 ctgtaacttt acctaagcag atataaatgg ctggttttta gaaaaaaaaa aaaaaaaaaa 900 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 955 <210> 73 <211> 77 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: oligonucleótido sintético <220> <223> 3xmiR-122BS <400> 73 cgaaacaaac accattgtca cactccaaca aacaccattg tcacactcca acaaacacca 60 ttgtcacact ccattcg 77 <210> 74 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 74 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 75 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3'ITR <400> 75 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 76 <211> 21 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: cebador sintético <220> <223> Glut1QPCR F1 <400> 76 cttgcttgta gagtgacgat c 21 <210> 77 <211> 20 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: cebador sintético <220> <223> Glut1QPCR R1 <400> 77 cagtgatccg agcactgctc 20 <210> 78 <211> 492 <212> PRT <213> Mus musculus <220> <223> secuencia de aminoácidos de mGlut1; familia portadora de solutos 2, miembro 1 del transportador de glucosa facilitado <400> 78 Met Asp Pro Ser Ser Lys Lys Val Thr Gly Arg Leu Met Leu Ala Val 1 5 10 15 Gly Gly Ala Val Leu Gly Ser Leu Gln Phe Gly Tyr Asn Thr Gly Val 20 25 30 Ile Asn Ala Pro Gln Lys Val Ile Glu Glu Phe Tyr Asn Gln Thr Trp 35 40 45 Asn His Arg Tyr Gly Glu Pro Ile Pro Ser Thr Thr Leu Thr Thr Leu 50 55 60 Trp Ser Leu Ser Val Ala Ile Phe Ser Val Gly Gly Met Ile Gly Ser 65 70 75 80 Phe Ser Val Gly Leu Phe Val Asn Arg Phe Gly Arg Arg Asn Ser Met 85 90 95 Leu Met Met Asn Leu Leu Ala Phe Val Ala Ala Val Leu Met Gly Phe 100 105 110 Ser Lys Leu Gly Lys Ser Phe Glu Met Leu Ile Leu Gly Arg Phe Ile 115 120 125 Ile Gly Val Tyr Cys Gly Leu Thr Thr Gly Phe Val Pro Met Tyr Val 130 135 140 Gly Glu Val Ser Pro Thr Ala Leu Arg Gly Ala Leu Gly Thr Leu His 145 150 155 160 Gln Leu Gly Ile Val Val Gly Ile Leu Ile Ala Gln Val Phe Gly Leu 165 170 175 Asp Ser Ile Met Gly Asn Ala Asp Leu Trp Pro Leu Leu Leu Ser Val 180 185 190 Ile Phe Ile Pro Ala Leu Leu Gln Cys Ile Leu Leu Pro Phe Cys Pro 195 200 205 Glu Ser Pro Arg Phe Leu Leu Ile Asn Arg Asn Glu Glu Asn Arg Ala 210 215 220 Lys Ser Val Leu Lys Lys Leu Arg Gly Thr Ala Asp Val Thr Arg Asp 225 230 235 240 Leu Gln Glu Met Lys Glu Glu Gly Arg Gln Met Met Arg Glu Lys Lys 245 250 255 Val Thr Ile Leu Glu Leu Phe Arg Ser Pro Ala Tyr Arg Gln Pro Ile 260 265 270 Leu Ile Ala Val Val Leu Gln Leu Ser Gln Gln Leu Ser Gly Ile Asn 275 280 285 Ala Val Phe Tyr Tyr Ser Thr Ser Ile Phe Glu Lys Ala Gly Val Gln 290 295 300 Gln Pro Val Tyr Ala Thr Ile Gly Ser Gly Ile Val Asn Thr Ala Phe 305 310 315 320 Thr Val Val Ser Leu Phe Val Val Glu Arg Ala Gly Arg Arg Thr Leu 325 330 335 His Leu Ile Gly Leu Ala Gly Met Ala Gly Cys Ala Val Leu Met Thr 340 345 350 Ile Ala Leu Ala Leu Leu Glu Arg Leu Pro Trp Met Ser Tyr Leu Ser 355 360 365 Ile Val Ala Ile Phe Gly Phe Val Ala Phe Phe Glu Val Gly Pro Gly 370 375 380 Pro Ile Pro Trp Phe Ile Val Ala Glu Leu Phe Ser Gln Gly Pro Arg 385 390 395 400 Pro Ala Ala Ile Ala Val Ala Gly Phe Ser Asn Trp Thr Ser Asn Phe 405 410 415 Ile Val Gly Met Cys Phe Gln Tyr Val Glu Gln Leu Cys Gly Pro Tyr 420 425 430 Val Phe Ile Ile Phe Thr Val Leu Leu Val Leu Phe Phe Ile Phe Thr 435 440 445 Tyr Phe Lys Val Pro Glu Thr Lys Gly Arg Thr Phe Asp Glu Ile Ala 450 455 460 Ser Gly Phe Arg Gln Gly Gly Ala Ser Gln Ser Asp Lys Thr Pro Glu 465 470 475 480 Glu Leu Phe His Pro Leu Gly Ala Asp Ser Gln Val 485 490 <210> 79 <211> 492 <212> PRT <213> Homo sapiens <220> <223> secuencia de aminoácidos de hGlut1; familia portadora de solutos 2, miembro 1 del transportador de glucosa facilitado <400> 79 Met Glu Pro Ser Ser Lys Lys Leu Thr Gly Arg Leu Met Leu Ala Val 1 5 10 15 Gly Gly Ala Val Leu Gly Ser Leu Gln Phe Gly Tyr Asn Thr Gly Val 20 25 30 Ile Asn Ala Pro Gln Lys Val Ile Glu Glu Phe Tyr Asn Gln Thr Trp 35 40 45 Val His Arg Tyr Gly Glu Ser Ile Leu Pro Thr Thr Leu Thr Thr Leu 50 55 60 Trp Ser Leu Ser Val Ala Ile Phe Ser Val Gly Gly Met Ile Gly Ser 65 70 75 80 Phe Ser Val Gly Leu Phe Val Asn Arg Phe Gly Arg Arg Asn Ser Met 85 90 95 Leu Met Met Asn Leu Leu Ala Phe Val Ser Ala Val Leu Met Gly Phe 100 105 110 Ser Lys Leu Gly Lys Ser Phe Glu Met Leu Ile Leu Gly Arg Phe Ile 115 120 125 Ile Gly Val Tyr Cys Gly Leu Thr Thr Gly Phe Val Pro Met Tyr Val 130 135 140 Gly Glu Val Ser Pro Thr Ala Leu Arg Gly Ala Leu Gly Thr Leu His 145 150 155 160 Gln Leu Gly Ile Val Val Gly Ile Leu Ile Ala Gln Val Phe Gly Leu 165 170 175 Asp Ser Ile Met Gly Asn Lys Asp Leu Trp Pro Leu Leu Leu Ser Ile 180 185 190 Ile Phe Ile Pro Ala Leu Leu Gln Cys Ile Val Leu Pro Phe Cys Pro 195 200 205 Glu Ser Pro Arg Phe Leu Leu Ile Asn Arg Asn Glu Glu Asn Arg Ala 210 215 220 Lys Ser Val Leu Lys Lys Leu Arg Gly Thr Ala Asp Val Thr His Asp 225 230 235 240 Leu Gln Glu Met Lys Glu Glu Ser Arg Gln Met Met Arg Glu Lys Lys 245 250 255 Val Thr Ile Leu Glu Leu Phe Arg Ser Pro Ala Tyr Arg Gln Pro Ile 260 265 270 Leu Ile Ala Val Val Leu Gln Leu Ser Gln Gln Leu Ser Gly Ile Asn 275 280 285 Ala Val Phe Tyr Tyr Ser Thr Ser Ile Phe Glu Lys Ala Gly Val Gln 290 295 300 Gln Pro Val Tyr Ala Thr Ile Gly Ser Gly Ile Val Asn Thr Ala Phe 305 310 315 320 Thr Val Val Ser Leu Phe Val Val Glu Arg Ala Gly Arg Arg Thr Leu 325 330 335 His Leu Ile Gly Leu Ala Gly Met Ala Gly Cys Ala Ile Leu Met Thr 340 345 350 Ile Ala Leu Ala Leu Leu Glu Gln Leu Pro Trp Met Ser Tyr Leu Ser 355 360 365 Ile Val Ala Ile Phe Gly Phe Val Ala Phe Phe Glu Val Gly Pro Gly 370 375 380 Pro Ile Pro Trp Phe Ile Val Ala Glu Leu Phe Ser Gln Gly Pro Arg 385 390 395 400 Pro Ala Ala Ile Ala Val Ala Gly Phe Ser Asn Trp Thr Ser Asn Phe 405 410 415 Ile Val Gly Met Cys Phe Gln Tyr Val Glu Gln Leu Cys Gly Pro Tyr 420 425 430 Val Phe Ile Ile Phe Thr Val Leu Leu Val Leu Phe Phe Ile Phe Thr 435 440 445 Tyr Phe Lys Val Pro Glu Thr Lys Gly Arg Thr Phe Asp Glu Ile Ala 450 455 460 Ser Gly Phe Arg Gln Gly Gly Ala Ser Gln Ser Asp Lys Thr Pro Glu 465 470 475 480 Glu Leu Phe His Pro Leu Gly Ala Asp Ser Gln Val 485 490 <210> 80 <211> 6834 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI hGlut1- EGFP <400> 80 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 tcccagcagc aagaaggtga cgggccgcct catgttggct gtgggaggag cagtgctcgg 1440 atcactgcag ttcggctata acactggtgt catcaacgcc ccccagaagg ttattgagga 1500 gttctacaat caaacatgga accaccgcta cggagagccc atcccatcca ccacactcac 1560 cacgctttgg tctctctccg tggccatctt ctctgtcggg ggcatgattg gttccttctc 1620 tgtcggcctc tttgttaatc gctttggcag gcggaactcc atgctgatga tgaacctgtt 1680 ggcctttgtg gctgctgtgc ttatgggctt ctccaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg actactggct ttgtgcccat 1800 gtatgtggga gaggtgtcac ctacagctct acgtggagcc ctaggcacac tgcaccagct 1860 gggaatcgtc gttggcatcc ttattgccca ggtgtttggc ttagactcca tcatgggcaa 1920 tgcagacttg tggcctctgc tgctcagtgt catcttcatc ccagccctgc tacagtgtat 1980 cctgttgccc ttctgccccg agagcccccg cttcctgctc atcaatcgta acgaggagaa 2040 ccgggccaag agtgtgctga agaagcttcg agggacagcc gatgtgaccc gagacctgca 2100 ggagatgaaa gaagagggtc ggcagatgat gcgggagaag aaggtcacca tcttggagct 2160 gttccgctca cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtcg ggtatcaatg ctgtgttcta ctactcaacg agcatcttcg agaaggcagg 2280 tgtgcagcag cctgtgtacg ccaccatcgg ctccggtatc gtcaacacgg ccttcactgt 2340 ggtgtcgctg tttgttgtag agcgagctgg acgacggacc ctgcacctca ttggcctggc 2400 tggcatggca ggctgtgctg tgctcatgac catcgccctg gccttgctgg aacggctgcc 2460 ttggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtagg 2520 ccctggtcct attccatggt tcattgtggc cgagctgttc agccaggggc cccgtcctgc 2580 tgctattgct gtggctggct tctccaactg gacctcaaac ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gcggccccta cgtcttcatc atcttcacgg tgctcctcgt 2700 gctcttcttc atcttcacct acttcaaagt ccctgagacc aaaggccgaa ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggtgc cagccaaagt gacaagacac ccgaggagct 2820 cttccaccct ctgggggcgg actcccaagt gaccggtgcc atggtgagca agggcgagga 2880 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 2940 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 3000 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta 3060 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 3120 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 3180 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 3240 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa 3300 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa 3360 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 3420 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 3480 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 3540 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccatc aagcttatcg 3600 ggccgctcta gagtatccct cgactctaga gtcgacccgg gcggcctcga ggacggggtg 3660 aactacgcct gaggatccga tctttttccc tctgccaaaa attatgggga catcatgaag 3720 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt 3780 tggaattttt tgtgtctctc actcggaagc aattcgttga tctgaatttc gaccacccat 3840 aatacccatt accctggtag ataagtagca tggcgggtta atcattaact acaaggaacc 3900 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 3960 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 4020 cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 4080 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 4140 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 4200 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 4260 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4320 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 4380 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4440 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4500 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4560 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4620 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 4680 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 4740 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 4800 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 4860 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 4920 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 4980 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 5040 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 5100 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 5160 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 5220 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 5280 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 5340 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 5400 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 5460 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 5520 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 5580 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 5640 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 5700 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 5760 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 5820 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 5880 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 5940 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 6000 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6060 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6120 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6180 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6240 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6300 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6360 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6420 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 6480 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 6540 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 6600 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 6660 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 6720 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 6780 caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta aggc 6834 <210> 81 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: sintético <220> <223> 5'ITR <400> 81 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 82 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 82 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 83 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 83 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 84 <211> 717 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> eGFP <400> 84 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 85 <211> 1959 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de hGlut1 y 3'UTR <400> 85 atggagccca gcagcaagaa gctgacgggt cgcctcatgc tggccgtggg aggagcagtg 60 cttggctccc tgcagtttgg ctacaacact ggagtcatca atgcccccca gaaggtgatc 120 gaggagttct acaaccagac atgggtccac cgctatgggg agagcatcct gcccaccacg 180 ctcaccacgc tctggtccct ctcagtggcc atcttttctg ttgggggcat gattggctcc 240 ttctctgtgg gccttttcgt taaccgcttt ggccggcgga attcaatgct gatgatgaac 300 ctgctggcct tcgtgtccgc cgtgctcatg ggcttctcga aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgtg gcctgaccac aggcttcgtg 420 cccatgtatg tgggtgaagt gtcacccaca gcccttcgtg gggccctggg caccctgcac 480 cagctgggca tcgtcgtcgg catcctcatc gcccaggtgt tcggcctgga ctccatcatg 540 ggcaacaagg acctgtggcc cctgctgctg agcatcatct tcatcccggc cctgctgcag 600 tgcatcgtgc tgcccttctg ccccgagagt ccccgcttcc tgctcatcaa ccgcaacgag 660 gagaaccggg ccaagagtgt gctaaagaag ctgcgcggga cagctgacgt gacccatgac 720 ctgcaggaga tgaaggaaga gagtcggcag atgatgcggg agaagaaggt caccatcctg 780 gagctgttcc gctcccccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtctggcat caacgctgtc ttctattact ccacgagcat cttcgagaag 900 gcgggggtgc agcagcctgt gtatgccacc attggctccg gtatcgtcaa cacggccttc 960 actgtcgtgt cgctgtttgt ggtggagcga gcaggccggc ggaccctgca cctcataggc 1020 ctcgctggca tggcgggttg tgccatactc atgaccatcg cgctagcact gctggagcag 1080 ctaccccgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtgggtcctg gccccatccc atggttcatc gtggctgaac tcttcagcca gggtccacgt 1200 ccagctgcca ttgccgttgc aggcttctcc aactggacct caaatttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgtggt ccctacgtct tcatcatctt cactgtgctc 1320 ctggttctgt tcttcatctt cacctacttc aaagttcctg agactaaagg ccggaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggagccagcc aaagtgacaa gacacccgag 1440 gagctgttcc atcccctggg ggctgattcc caagtgtgag tcgccccaga tcaccagccc 1500 ggcctgctcc cagcagccct aaggatctct caggagcaca ggcagctgga tgagacttcc 1560 aaacctgaca gatgtcagcc gagccgggcc tggggctcct ttctccagcc agcaatgatg 1620 tccagaagaa tattcaggac ttaacggctc caggatttta acaaaagcaa gactgttgct 1680 caaatctatt cagacaagca acaggtttta taattttttt attactgatt ttgttatttt 1740 tatatcagcc tgagtctcct gtgcccacat cccaggcttc accctgaatg gttccatgcc 1800 tgagggtgga gactaagccc tgtcgagaca cttgccttct tcacccagct aatctgtagg 1860 gctggaccta tgtcctaagg acacactaat cgaactatga actacaaagc ttctatccca 1920 ggaggtggct atggccaccc gttctgctgg cctggatct 1959 <210> 86 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 86 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 87 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 87 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 88 <211> 6885 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> pAAV CB6 PI mGlut1-2A- EGFP <400> 88 cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 60 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 120 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag 180 ggtaatgggg atcctctaga actatagcta gtcgacattg attattgact agttattaat 240 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac 300 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa 360 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt 420 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc 480 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat 540 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atgtcgaggc 600 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt 660 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc gccaggcggg 720 gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag 780 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 840 aagcgaagcg cgcggcgggc gggagcaagc tttattgcgg tagtttatca cagttaaatt 900 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg 960 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa 1020 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact 1080 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa 1140 ggctagagta cttaatacga ctcactatag gctagcgcgc cgaattcggc acgaggaaaa 1200 aggcagctcc gcgcgctctc ccccaagagc agaggcttgc ttgtagagtg acgatctgag 1260 ctacggggtc ttaagtgcgt cagggcgtgg aggtctggcg ggagacgcat agttacagcg 1320 cgtccgttct ccgtctcgca gccggcacag ctagagcttc gagcgcagcg cggccatgga 1380 tcccagcagc aagaaggtga cgggccgcct catgttggct gtgggaggag cagtgctcgg 1440 atcactgcag ttcggctata acactggtgt catcaacgcc ccccagaagg ttattgagga 1500 gttctacaat caaacatgga accaccgcta cggagagccc atcccatcca ccacactcac 1560 cacgctttgg tctctctccg tggccatctt ctctgtcggg ggcatgattg gttccttctc 1620 tgtcggcctc tttgttaatc gctttggcag gcggaactcc atgctgatga tgaacctgtt 1680 ggcctttgtg gctgctgtgc ttatgggctt ctccaaactg ggcaagtcct ttgagatgct 1740 gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg actactggct ttgtgcccat 1800 gtatgtggga gaggtgtcac ctacagctct acgtggagcc ctaggcacac tgcaccagct 1860 gggaatcgtc gttggcatcc ttattgccca ggtgtttggc ttagactcca tcatgggcaa 1920 tgcagacttg tggcctctgc tgctcagtgt catcttcatc ccagccctgc tacagtgtat 1980 cctgttgccc ttctgccccg agagcccccg cttcctgctc atcaatcgta acgaggagaa 2040 ccgggccaag agtgtgctga agaagcttcg agggacagcc gatgtgaccc gagacctgca 2100 ggagatgaaa gaagagggtc ggcagatgat gcgggagaag aaggtcacca tcttggagct 2160 gttccgctca cccgcctacc gccagcccat cctcatcgct gtggtgctgc agctgtccca 2220 gcagctgtcg ggtatcaatg ctgtgttcta ctactcaacg agcatcttcg agaaggcagg 2280 tgtgcagcag cctgtgtacg ccaccatcgg ctccggtatc gtcaacacgg ccttcactgt 2340 ggtgtcgctg tttgttgtag agcgagctgg acgacggacc ctgcacctca ttggcctggc 2400 tggcatggca ggctgtgctg tgctcatgac catcgccctg gccttgctgg aacggctgcc 2460 ttggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct ttgaagtagg 2520 ccctggtcct attccatggt tcattgtggc cgagctgttc agccaggggc cccgtcctgc 2580 tgctattgct gtggctggct tctccaactg gacctcaaac ttcattgtgg gcatgtgctt 2640 ccagtatgtg gagcaactgt gcggccccta cgtcttcatc atcttcacgg tgctcctcgt 2700 gctcttcttc atcttcacct acttcaaagt ccctgagacc aaaggccgaa ccttcgatga 2760 gatcgcttcc ggcttccggc aggggggtgc cagccaaagt gacaagacac ccgaggagct 2820 cttccaccct ctgggggcgg actcccaagt gaccggtaat tttgaccttc ttaagcttgc 2880 gggagacgtc gagtccaacc ctgggcccgc catggtgagc aagggcgagg agctgttcac 2940 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt 3000 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac 3060 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca 3120 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc 3180 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg 3240 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga 3300 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa 3360 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca 3420 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg 3480 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa 3540 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat 3600 cactctcggc atggacgagc tgtacaagta aagcggccat caagcttatc gggccgctct 3660 agagtatccc tcgactctag agtcgacccg ggcggcctcg aggacggggt gaactacgcc 3720 tgaggatccg atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag 3780 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt 3840 ttgtgtctct cactcggaag caattcgttg atctgaattt cgaccaccca taatacccat 3900 taccctggta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat 3960 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 4020 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa 4080 ttaacctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 4140 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 4200 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg 4260 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 4320 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 4380 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 4440 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 4500 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 4560 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 4620 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 4680 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc 4740 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 4800 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 4860 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 4920 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 4980 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 5040 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 5100 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 5160 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 5220 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 5280 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 5340 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 5400 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 5460 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 5520 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 5580 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 5640 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 5700 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 5760 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 5820 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 5880 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 5940 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 6000 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 6060 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 6120 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 6180 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 6240 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 6300 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 6360 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 6420 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 6480 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 6540 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 6600 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 6660 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 6720 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct 6780 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 6840 acaggaaaca gctatgacca tgattacgcc agatttaatt aaggc 6885 <210> 89 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 5'ITR <400> 89 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct 130 <210> 90 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Potenciador de CMV IE <400> 90 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 91 <211> 382 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Promotor de CB <400> 91 ctagtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 60 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 120 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 180 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 240 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 300 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 360 acgtattagt catcgctatt ac 382 <210> 92 <211> 717 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> eGFP <400> 92 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaag 717 <210> 93 <211> 1476 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADNc de mGlut1 y 3'UTR <400> 93 atggatccca gcagcaagaa ggtgacgggc cgcctcatgt tggctgtggg aggagcagtg 60 ctcggatcac tgcagttcgg ctataacact ggtgtcatca acgcccccca gaaggttatt 120 gaggagttct acaatcaaac atggaaccac cgctacggag agcccatccc atccaccaca 180 ctcaccacgc tttggtctct ctccgtggcc atcttctctg tcgggggcat gattggttcc 240 ttctctgtcg gcctctttgt taatcgcttt ggcaggcgga actccatgct gatgatgaac 300 ctgttggcct ttgtggctgc tgtgcttatg ggcttctcca aactgggcaa gtcctttgag 360 atgctgatcc tgggccgctt catcatcggt gtgtactgcg gcctgactac tggctttgtg 420 cccatgtatg tgggagaggt gtcacctaca gctctacgtg gagccctagg cacactgcac 480 cagctgggaa tcgtcgttgg catccttatt gcccaggtgt ttggcttaga ctccatcatg 540 ggcaatgcag acttgtggcc tctgctgctc agtgtcatct tcatcccagc cctgctacag 600 tgtatcctgt tgcccttctg ccccgagagc ccccgcttcc tgctcatcaa tcgtaacgag 660 gagaaccggg ccaagagtgt gctgaagaag cttcgaggga cagccgatgt gacccgagac 720 ctgcaggaga tgaaagaaga gggtcggcag atgatgcggg agaagaaggt caccatcttg 780 gagctgttcc gctcacccgc ctaccgccag cccatcctca tcgctgtggt gctgcagctg 840 tcccagcagc tgtcgggtat caatgctgtg ttctactact caacgagcat cttcgagaag 900 gcaggtgtgc agcagcctgt gtacgccacc atcggctccg gtatcgtcaa cacggccttc 960 actgtggtgt cgctgtttgt tgtagagcga gctggacgac ggaccctgca cctcattggc 1020 ctggctggca tggcaggctg tgctgtgctc atgaccatcg ccctggcctt gctggaacgg 1080 ctgccttgga tgtcctatct gagcatcgtg gccatctttg gctttgtggc cttctttgaa 1140 gtaggccctg gtcctattcc atggttcatt gtggccgagc tgttcagcca ggggccccgt 1200 cctgctgcta ttgctgtggc tggcttctcc aactggacct caaacttcat tgtgggcatg 1260 tgcttccagt atgtggagca actgtgcggc ccctacgtct tcatcatctt cacggtgctc 1320 ctcgtgctct tcttcatctt cacctacttc aaagtccctg agaccaaagg ccgaaccttc 1380 gatgagatcg cttccggctt ccggcagggg ggtgccagcc aaagtgacaa gacacccgag 1440 gagctcttcc accctctggg ggcggactcc caagtg 1476 <210> 94 <211> 127 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> Señal Poli A <400> 94 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 60 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 120 tcactcg 127 <210> 95 <211> 130 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> 3' ITR <400> 95 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag 130 <210> 96 <211> 2208 <212> ADN <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polinucleótido sintético <220> <223> ADN de AAV9 <400> 96 atggctgccg atggttatct tccagattgg ctcgaggaca accttagtga aggaattcgc 60 gagtggtggg ctttgaaacc tggagcccct caacccaagg caaatcaaca acatcaagac 120 aacgctcgag gtcttgtgct tccgggttac aaataccttg gacccggcaa cggactcgac 180 aagggggagc cggtcaacgc agcagacgcg gcggccctcg agcacgacaa ggcctacgac 240 cagcagctca aggccggaga caacccgtac ctcaagtaca accacgccga cgccgagttc 300 caggagcggc tcaaagaaga tacgtctttt gggggcaacc tcgggcgagc agtcttccag 360 gccaaaaaga ggcttcttga acctcttggt ctggttgagg aagcggctaa gacggctcct 420 ggaaagaaga ggcctgtaga gcagtctcct caggaaccgg actcctccgc gggtattggc 480 aaatcgggtg cacagcccgc taaaaagaga ctcaatttcg gtcagactgg cgacacagag 540 tcagtcccag accctcaacc aatcggagaa cctcccgcag ccccctcagg tgtgggatct 600 cttacaatgg cttcaggtgg tggcgcacca gtggcagaca ataacgaagg tgccgatgga 660 gtgggtagtt cctcgggaaa ttggcattgc gattcccaat ggctggggga cagagtcatc 720 accaccagca cccgaacctg ggccctgccc acctacaaca atcacctcta caagcaaatc 780 tccaacagca catctggagg atcttcaaat gacaacgcct acttcggcta cagcaccccc 840 tgggggtatt ttgacttcaa cagattccac tgccacttct caccacgtga ctggcagcga 900 ctcatcaaca acaactgggg attccggcct aagcgactca acttcaagct cttcaacatt 960 caggtcaaag aggttacgga caacaatgga gtcaagacca tcgccaataa ccttaccagc 1020 acggtccagg tcttcacgga ctcagactat cagctcccgt acgtgctcgg gtcggctcac 1080 gagggctgcc tcccgccgtt cccagcggac gttttcatga ttcctcagta cgggtatctg 1140 acgcttaatg atggaagcca ggccgtgggt cgttcgtcct tttactgcct ggaatatttc 1200 ccgtcgcaaa tgctaagaac gggtaacaac ttccagttca gctacgagtt tgagaacgta 1260 cctttccata gcagctacgc tcacagccaa agcctggacc gactaatgaa tccactcatc 1320 gaccaatact tgtactatct ctcaaagact attaacggtt ctggacagaa tcaacaaacg 1380 ctaaaattca gtgtggccgg acccagcaac atggctgtcc agggaagaaa ctacatacct 1440 ggacccagct accgacaaca acgtgtctca accactgtga ctcaaaacaa caacagcgaa 1500 tttgcttggc ctggagcttc ttcttgggct ctcaatggac gtaatagctt gatgaatcct 1560 ggacctgcta tggccagcca caaagaagga gaggaccgtt tctttccttt gtctggatct 1620 ttaatttttg gcaaacaagg aactggaaga gacaacgtgg atgcggacaa agtcatgata 1680 accaacgaag aagaaattaa aactactaac ccggtagcaa cggagtccta tggacaagtg 1740 gccacaaacc accagagtgc ccaagcacag gcgcagaccg gctgggttca aaaccaagga 1800 atacttccgg gtatggtttg gcaggacaga gatgtgtacc tgcaaggacc catttgggcc 1860 aaaattcctc acacggacgg caactttcac ccttctccgc tgatgggagg gtttggaatg 1920 aagcacccgc ctcctcagat cctcatcaaa aacacacctg tacctgcgga tcctccaacg 1980 gccttcaaca aggacaagct gaactctttc atcacccagt attctactgg ccaagtcagc 2040 gtggagatcg agtgggagct gcagaaggaa aacagcaagc gctggaaccc ggagatccag 2100 tacacttcca actattacaa gtctaataat gttgaatttg ctgttaatac tgaaggtgta 2160 tatagtgaac cccgccccat tggcaccaga tacctgactc gtaatctg 2208 <210> 97 <211> 736 <212> PRT <213> Secuencia artificial <220> <223> Descripción de secuencia artificial: polipéptido sintético <220> <223> Proteína de AAV9 <400> 97 Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 1 5 10 15 Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 20 25 30 Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 35 40 45 Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 50 55 60 Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 65 70 75 80 Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 85 90 95 Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 100 105 110 Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 115 120 125 Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 130 135 140 Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 145 150 155 160 Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 165 170 175 Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 180 185 190 Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 195 200 205 Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 210 215 220 Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 225 230 235 240 Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 245 250 255 Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 260 265 270 Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 275 280 285 Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 290 295 300 Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 305 310 315 320 Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 325 330 335 Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 340 345 350 Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 355 360 365 Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 370 375 380 Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 385 390 395 400 Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 405 410 415 Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 420 425 430 Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 435 440 445 Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 450 455 460 Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 465 470 475 480 Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 485 490 495 Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 500 505 510 Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 515 520 525 Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 530 535 540 Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 545 550 555 560 Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 565 570 575 Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 580 585 590 Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 595 600 605 Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 610 615 620 Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 625 630 635 640 Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 645 650 655 Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 660 665 670 Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 675 680 685 Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 690 695 700 Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 705 710 715 720 Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 725 730 735 2