LISTADO DE SECUENCIAS <110> Aduro Biotech, Inc. <120> FUSIONES DE VARIANTE III DE RECEPTOR DE FACTOR DE CRECIMIENTO EPIDÉRMICO-MESOTELINA Y MÉTODOS PARA USAR LOS MISMOS <130> 5316.1000-003 <150> 62/146,559 <151> 2015-04-13 <150> 62/243,397 <151> 2015-10-19 <160> 32 <170> FastSEQ para Windows Versión 4.0 <210> 1 <211> 2744 <212> ADN <213> Secuencia artificial <220> <223> fusión <400> 1 gggaagcagt tggggttaac tgattaacaa atgttagaga aaaattaatt ctccaagtga 60 tattcttaaa ataattcatg aatatttttt cttatattag ctaattaaga agataattaa 120 ctgctaatcc aatttttaac ggaataaatt agtgaaaatg aaggccgaat tttccttgtt 180 ctaaaaaggt tgtattagcg tatcacgagg agggagtata agtgggatta aatagattta 240 tgcgtgcgat gatggtagtt ttcattactg ccaactgcat tacgattaac cccgacataa 300 tatttgcagc gacagatagc gaagattcca gtctaaacac agatgaatgg gaagaagaat 360 acgaaactgc acgtgaagta agttcacgtg atattgagga actagaaaaa tcgaataaag 420 tgaaaaatac gaacaaagca gaccaagata ataaacgtaa agcaaaagca gagaaaggtg 480 gatccgcaag caaagtattg ccagctagtc gtgcattaga ggagaaaaag gggaattacg 540 tggtgacgga tcatggatcg tgtgccgatg gctcagtaaa gactagtgcg agcaaagtgg 600 cccctgcatc acgagcactt gaagagaaaa aaggaaacta tgttgtgacc gatcatggta 660 gctgcggaga tggttcaatt aaattatcaa aagtcttacc agcatctaga gctttagagg 720 aaaagaaggg taactatgtc gtaacagatc atggaagttg tgctgacgga agtgttaaag 780 cgtcgaaagt agctccagct tctcgcgcat tagaagaaaa gaaaggcaat tatgttgtaa 840 cagaccatgg tagttgtggt gatggctcga tcaaattgtc aaaagttcta ccggcttctc 900 gtgcgctaga agagaagaaa ggaaattacg tagttacaga ccacggctct tgcgcggatg 960 gttccgttaa acaattgcgt acattagcag gtgaaacagg tcaagaagca gcaccacttg 1020 acggtgtatt aacgaatcca ccaaatatat caagtttaag tccacgtcaa ttattaggtt 1080 ttccatgtgc agaagtttca ggtttaagta cagaacgtgt ccgtgagtta gcagttgcat 1140 tagcacaaaa aaacgttaaa ttatctacag aacagttacg ttgtttagcc catagattaa 1200 gcgaaccacc agaagactta gatgcacttc ctttagacct tcttttattc ttaaatccag 1260 atgcattttc aggaccacaa gcatgtacac gtttttttag tcgaattaca aaagccaatg 1320 ttgatttatt acctcgtggg gctcctgaaa gacaacgttt attacctgct gcattagcat 1380 gctggggtgt tcgcggtagc ttattaagtg aagccgatgt tcgtgcttta gggggtttag 1440 catgtgattt acctggtcgt ttcgttgcag aatcagcaga agtgttatta ccgagattag 1500 tttcatgccc aggaccttta gatcaagatc aacaagaggc agctagagca gctcttcaag 1560 gaggaggccc accatatggc ccaccaagta catggagtgt ttctacaatg gatgcgttaa 1620 gaggtttatt accggtttta ggacaaccaa ttattcgtag tattccacaa ggcattgtag 1680 cagcatggcg tcaacgtagt tctcgtgatc cgtcttggcg acaaccagaa cgtacaattc 1740 tacgtccaag atttcgtaga gaagtagaaa aaacggcgtg tcctagtggc aaaaaagcac 1800 gtgaaattga tgaaagttta attttttata aaaaatggga attagaagca tgtgtcgatg 1860 cagcattact agctacacaa atggatcgtg ttaatgctat tccattcaca tatgaacaat 1920 tagatgtttt aaagcataaa ttagacgaat tatatccaca aggttatcca gaatcagtta 1980 ttcaacattt aggttactta tttttaaaaa tgagtccaga agacatacgc aaatggaatg 2040 ttacaagttt agaaacatta aaagcgcttt tagaagttaa caaaggtcat gaaatgagtc 2100 cacaagttgc tacgttaatt gatagattcg ttaaaggccg tggtcaatta gataaagata 2160 ctttagatac attaacagca ttttatcctg gctacttatg cagtttatca ccagaagaat 2220 taagttccgt tccaccgagt agtatctggg cagttcgtcc gcaagattta gatacatgcg 2280 acccacgtca attagatgtt ttatatccaa aagcaagatt agctttccaa aatatgaacg 2340 gtagtgaata tttcgtaaaa attcaatcct ttttaggtgg tgcaccaact gaagatctaa 2400 aagcattaag ccaacaaaat gtaagtatgg atttagctac gtttatgaaa ttacgtacag 2460 atgcagttct accattaaca gttgcagaag ttcaaaaatt attaggtcca cacgtagaag 2520 gattaaaagc agaagaacgt caccgtccag ttcgcgattg gattttacgt caacgtcaag 2580 atgatttaga tacattaggt ttaggtttac aaggcggtat tccgaatgga tatttagtgt 2640 tagatttatc ggttcaagaa gcattaagtg gtacaccgtg tttattaggt ccaggtccag 2700 ttttaacagt gttagcatta ttattagcca gtacattagc ttaa 2744 <210> 2 <211> 221 <212> ADN <213> Secuencia artificial <220> <223> promotor <400> 2 gggaagcagt tggggttaac tgattaacaa atgttagaga aaaattaatt ctccaagtga 60 tattcttaaa ataattcatg aatatttttt cttatattag ctaattaaga agataattaa 120 ctgctaatcc aatttttaac ggaataaatt agtgaaaatg aaggccgaat tttccttgtt 180 ctaaaaaggt tgtattagcg tatcacgagg agggagtata a 221 <210> 3 <211> 258 <212> ADN <213> Secuencia artificial <220> <223> promotor <400> 3 gtgggattaa atagatttat gcgtgcgatg atggtagttt tcattactgc caactgcatt 60 acgattaacc ccgacataat atttgcagcg acagatagcg aagattccag tctaaacaca 120 gatgaatggg aagaagaata cgaaactgca cgtgaagtaa gttcacgtga tattgaggaa 180 ctagaaaaat cgaataaagt gaaaaatacg aacaaagcag accaagataa taaacgtaaa 240 gcaaaagcag agaaaggt 258 <210> 4 <211> 63 <212> ADN <213> Secuencia artificial <220> <221> misc_feature <222> 33, 42 <223> n = A, T, C o G <220> <223> Secuencia consenso de EGFRvIII <400> 4 ccdgcwwswm ghgcdytwga rgaraaraar ggnaaytayg tngtdacvga ycaygghwsb 60 tgy 63 <210> 5 <211> 486 <212> ADN <213> Secuencia artificial <220> <223> fusión <400> 5 gcaagcaaag tattgccagc tagtcgtgca ttagaggaga aaaaggggaa ttacgtggtg 60 acggatcatg gatcgtgtgc cgatggctca gtaaagacta gtgcgagcaa agtggcccct 120 gcatcacgag cacttgaaga gaaaaaagga aactatgttg tgaccgatca tggtagctgc 180 ggagatggtt caattaaatt atcaaaagtc ttaccagcat ctagagcttt agaggaaaag 240 aagggtaact atgtcgtaac agatcatgga agttgtgctg acggaagtgt taaagcgtcg 300 aaagtagctc cagcttctcg cgcattagaa gaaaagaaag gcaattatgt tgtaacagac 360 catggtagtt gtggtgatgg ctcgatcaaa ttgtcaaaag ttctaccggc ttctcgtgcg 420 ctagaagaga agaaaggaaa ttacgtagtt acagaccacg gctcttgcgc ggatggttcc 480 gttaaa 486 <210> 6 <211> 1764 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 6 cgtacattag caggtgaaac aggtcaagaa gcagcaccac ttgacggtgt attaacgaat 60 ccaccaaata tatcaagttt aagtccacgt caattattag gttttccatg tgcagaagtt 120 tcaggtttaa gtacagaacg tgtccgtgag ttagcagttg cattagcaca aaaaaacgtt 180 aaattatcta cagaacagtt acgttgttta gcccatagat taagcgaacc accagaagac 240 ttagatgcac ttcctttaga ccttctttta ttcttaaatc cagatgcatt ttcaggacca 300 caagcatgta cacgtttttt tagtcgaatt acaaaagcca atgttgattt attacctcgt 360 ggggctcctg aaagacaacg tttattacct gctgcattag catgctgggg tgttcgcggt 420 agcttattaa gtgaagccga tgttcgtgct ttagggggtt tagcatgtga tttacctggt 480 cgtttcgttg cagaatcagc agaagtgtta ttaccgagat tagtttcatg cccaggacct 540 ttagatcaag atcaacaaga ggcagctaga gcagctcttc aaggaggagg cccaccatat 600 ggcccaccaa gtacatggag tgtttctaca atggatgcgt taagaggttt attaccggtt 660 ttaggacaac caattattcg tagtattcca caaggcattg tagcagcatg gcgtcaacgt 720 agttctcgtg atccgtcttg gcgacaacca gaacgtacaa ttctacgtcc aagatttcgt 780 agagaagtag aaaaaacggc gtgtcctagt ggcaaaaaag cacgtgaaat tgatgaaagt 840 ttaatttttt ataaaaaatg ggaattagaa gcatgtgtcg atgcagcatt actagctaca 900 caaatggatc gtgttaatgc tattccattc acatatgaac aattagatgt tttaaagcat 960 aaattagacg aattatatcc acaaggttat ccagaatcag ttattcaaca tttaggttac 1020 ttatttttaa aaatgagtcc agaagacata cgcaaatgga atgttacaag tttagaaaca 1080 ttaaaagcgc ttttagaagt taacaaaggt catgaaatga gtccacaagt tgctacgtta 1140 attgatagat tcgttaaagg ccgtggtcaa ttagataaag atactttaga tacattaaca 1200 gcattttatc ctggctactt atgcagttta tcaccagaag aattaagttc cgttccaccg 1260 agtagtatct gggcagttcg tccgcaagat ttagatacat gcgacccacg tcaattagat 1320 gttttatatc caaaagcaag attagctttc caaaatatga acggtagtga atatttcgta 1380 aaaattcaat cctttttagg tggtgcacca actgaagatc taaaagcatt aagccaacaa 1440 aatgtaagta tggatttagc tacgtttatg aaattacgta cagatgcagt tctaccatta 1500 acagttgcag aagttcaaaa attattaggt ccacacgtag aaggattaaa agcagaagaa 1560 cgtcaccgtc cagttcgcga ttggatttta cgtcaacgtc aagatgattt agatacatta 1620 ggtttaggtt tacaaggcgg tattccgaat ggatatttag tgttagattt atcggttcaa 1680 gaagcattaa gtggtacacc gtgtttatta ggtccaggtc cagttttaac agtgttagca 1740 ttattattag ccagtacatt agct 1764 <210> 7 <211> 2256 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 7 gcaagcaaag tattgccagc tagtcgtgca ttagaggaga aaaaggggaa ttacgtggtg 60 acggatcatg gatcgtgtgc cgatggctca gtaaagacta gtgcgagcaa agtggcccct 120 gcatcacgag cacttgaaga gaaaaaagga aactatgttg tgaccgatca tggtagctgc 180 ggagatggtt caattaaatt atcaaaagtc ttaccagcat ctagagcttt agaggaaaag 240 aagggtaact atgtcgtaac agatcatgga agttgtgctg acggaagtgt taaagcgtcg 300 aaagtagctc cagcttctcg cgcattagaa gaaaagaaag gcaattatgt tgtaacagac 360 catggtagtt gtggtgatgg ctcgatcaaa ttgtcaaaag ttctaccggc ttctcgtgcg 420 ctagaagaga agaaaggaaa ttacgtagtt acagaccacg gctcttgcgc ggatggttcc 480 gttaaacaat tgcgtacatt agcaggtgaa acaggtcaag aagcagcacc acttgacggt 540 gtattaacga atccaccaaa tatatcaagt ttaagtccac gtcaattatt aggttttcca 600 tgtgcagaag tttcaggttt aagtacagaa cgtgtccgtg agttagcagt tgcattagca 660 caaaaaaacg ttaaattatc tacagaacag ttacgttgtt tagcccatag attaagcgaa 720 ccaccagaag acttagatgc acttccttta gaccttcttt tattcttaaa tccagatgca 780 ttttcaggac cacaagcatg tacacgtttt tttagtcgaa ttacaaaagc caatgttgat 840 ttattacctc gtggggctcc tgaaagacaa cgtttattac ctgctgcatt agcatgctgg 900 ggtgttcgcg gtagcttatt aagtgaagcc gatgttcgtg ctttaggggg tttagcatgt 960 gatttacctg gtcgtttcgt tgcagaatca gcagaagtgt tattaccgag attagtttca 1020 tgcccaggac ctttagatca agatcaacaa gaggcagcta gagcagctct tcaaggagga 1080 ggcccaccat atggcccacc aagtacatgg agtgtttcta caatggatgc gttaagaggt 1140 ttattaccgg ttttaggaca accaattatt cgtagtattc cacaaggcat tgtagcagca 1200 tggcgtcaac gtagttctcg tgatccgtct tggcgacaac cagaacgtac aattctacgt 1260 ccaagatttc gtagagaagt agaaaaaacg gcgtgtccta gtggcaaaaa agcacgtgaa 1320 attgatgaaa gtttaatttt ttataaaaaa tgggaattag aagcatgtgt cgatgcagca 1380 ttactagcta cacaaatgga tcgtgttaat gctattccat tcacatatga acaattagat 1440 gttttaaagc ataaattaga cgaattatat ccacaaggtt atccagaatc agttattcaa 1500 catttaggtt acttattttt aaaaatgagt ccagaagaca tacgcaaatg gaatgttaca 1560 agtttagaaa cattaaaagc gcttttagaa gttaacaaag gtcatgaaat gagtccacaa 1620 gttgctacgt taattgatag attcgttaaa ggccgtggtc aattagataa agatacttta 1680 gatacattaa cagcatttta tcctggctac ttatgcagtt tatcaccaga agaattaagt 1740 tccgttccac cgagtagtat ctgggcagtt cgtccgcaag atttagatac atgcgaccca 1800 cgtcaattag atgttttata tccaaaagca agattagctt tccaaaatat gaacggtagt 1860 gaatatttcg taaaaattca atccttttta ggtggtgcac caactgaaga tctaaaagca 1920 ttaagccaac aaaatgtaag tatggattta gctacgttta tgaaattacg tacagatgca 1980 gttctaccat taacagttgc agaagttcaa aaattattag gtccacacgt agaaggatta 2040 aaagcagaag aacgtcaccg tccagttcgc gattggattt tacgtcaacg tcaagatgat 2100 ttagatacat taggtttagg tttacaaggc ggtattccga atggatattt agtgttagat 2160 ttatcggttc aagaagcatt aagtggtaca ccgtgtttat taggtccagg tccagtttta 2220 acagtgttag cattattatt agccagtaca ttagct 2256 <210> 8 <211> 840 <212> PRT <213> Secuencia artificial <220> <223> fusión <400> 8 Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 1 5 10 15 Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 20 25 30 Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Tyr Glu 35 40 45 Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser 50 55 60 Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Gln Asp Asn Lys Arg Lys 65 70 75 80 Ala Lys Ala Glu Lys Gly Gly Ser Ala Ser Lys Val Leu Pro Ala Ser 85 90 95 Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp His Gly 100 105 110 Ser Cys Ala Asp Gly Ser Val Lys Thr Ser Ala Ser Lys Val Ala Pro 115 120 125 Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp 130 135 140 His Gly Ser Cys Gly Asp Gly Ser Ile Lys Leu Ser Lys Val Leu Pro 145 150 155 160 Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp 165 170 175 His Gly Ser Cys Ala Asp Gly Ser Val Lys Ala Ser Lys Val Ala Pro 180 185 190 Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp 195 200 205 His Gly Ser Cys Gly Asp Gly Ser Ile Lys Leu Ser Lys Val Leu Pro 210 215 220 Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp 225 230 235 240 His Gly Ser Cys Ala Asp Gly Ser Val Lys Gln Leu Arg Thr Leu Ala 245 250 255 Gly Glu Thr Gly Gln Glu Ala Ala Pro Leu Asp Gly Val Leu Thr Asn 260 265 270 Pro Pro Asn Ile Ser Ser Leu Ser Pro Arg Gln Leu Leu Gly Phe Pro 275 280 285 Cys Ala Glu Val Ser Gly Leu Ser Thr Glu Arg Val Arg Glu Leu Ala 290 295 300 Val Ala Leu Ala Gln Lys Asn Val Lys Leu Ser Thr Glu Gln Leu Arg 305 310 315 320 Cys Leu Ala His Arg Leu Ser Glu Pro Pro Glu Asp Leu Asp Ala Leu 325 330 335 Pro Leu Asp Leu Leu Leu Phe Leu Asn Pro Asp Ala Phe Ser Gly Pro 340 345 350 Gln Ala Cys Thr Arg Phe Phe Ser Arg Ile Thr Lys Ala Asn Val Asp 355 360 365 Leu Leu Pro Arg Gly Ala Pro Glu Arg Gln Arg Leu Leu Pro Ala Ala 370 375 380 Leu Ala Cys Trp Gly Val Arg Gly Ser Leu Leu Ser Glu Ala Asp Val 385 390 395 400 Arg Ala Leu Gly Gly Leu Ala Cys Asp Leu Pro Gly Arg Phe Val Ala 405 410 415 Glu Ser Ala Glu Val Leu Leu Pro Arg Leu Val Ser Cys Pro Gly Pro 420 425 430 Leu Asp Gln Asp Gln Gln Glu Ala Ala Arg Ala Ala Leu Gln Gly Gly 435 440 445 Gly Pro Pro Tyr Gly Pro Pro Ser Thr Trp Ser Val Ser Thr Met Asp 450 455 460 Ala Leu Arg Gly Leu Leu Pro Val Leu Gly Gln Pro Ile Ile Arg Ser 465 470 475 480 Ile Pro Gln Gly Ile Val Ala Ala Trp Arg Gln Arg Ser Ser Arg Asp 485 490 495 Pro Ser Trp Arg Gln Pro Glu Arg Thr Ile Leu Arg Pro Arg Phe Arg 500 505 510 Arg Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys Lys Ala Arg Glu 515 520 525 Ile Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu Leu Glu Ala Cys 530 535 540 Val Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg Val Asn Ala Ile 545 550 555 560 Pro Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His Lys Leu Asp Glu 565 570 575 Leu Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln His Leu Gly Tyr 580 585 590 Leu Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys Trp Asn Val Thr 595 600 605 Ser Leu Glu Thr Leu Lys Ala Leu Leu Glu Val Asn Lys Gly His Glu 610 615 620 Met Ser Pro Gln Val Ala Thr Leu Ile Asp Arg Phe Val Lys Gly Arg 625 630 635 640 Gly Gln Leu Asp Lys Asp Thr Leu Asp Thr Leu Thr Ala Phe Tyr Pro 645 650 655 Gly Tyr Leu Cys Ser Leu Ser Pro Glu Glu Leu Ser Ser Val Pro Pro 660 665 670 Ser Ser Ile Trp Ala Val Arg Pro Gln Asp Leu Asp Thr Cys Asp Pro 675 680 685 Arg Gln Leu Asp Val Leu Tyr Pro Lys Ala Arg Leu Ala Phe Gln Asn 690 695 700 Met Asn Gly Ser Glu Tyr Phe Val Lys Ile Gln Ser Phe Leu Gly Gly 705 710 715 720 Ala Pro Thr Glu Asp Leu Lys Ala Leu Ser Gln Gln Asn Val Ser Met 725 730 735 Asp Leu Ala Thr Phe Met Lys Leu Arg Thr Asp Ala Val Leu Pro Leu 740 745 750 Thr Val Ala Glu Val Gln Lys Leu Leu Gly Pro His Val Glu Gly Leu 755 760 765 Lys Ala Glu Glu Arg His Arg Pro Val Arg Asp Trp Ile Leu Arg Gln 770 775 780 Arg Gln Asp Asp Leu Asp Thr Leu Gly Leu Gly Leu Gln Gly Gly Ile 785 790 795 800 Pro Asn Gly Tyr Leu Val Leu Asp Leu Ser Val Gln Glu Ala Leu Ser 805 810 815 Gly Thr Pro Cys Leu Leu Gly Pro Gly Pro Val Leu Thr Val Leu Ala 820 825 830 Leu Leu Leu Ala Ser Thr Leu Ala 835 840 <210> 9 <211> 21 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 9 Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr 1 5 10 15 Asp His Gly Ser Cys 20 <210> 10 <211> 162 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 10 Ala Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser Val Lys 20 25 30 Thr Ser Ala Ser Lys Val Ala Pro Ala Ser Arg Ala Leu Glu Glu Lys 35 40 45 Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys Gly Asp Gly Ser 50 55 60 Ile Lys Leu Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys 65 70 75 80 Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser 85 90 95 Val Lys Ala Ser Lys Val Ala Pro Ala Ser Arg Ala Leu Glu Glu Lys 100 105 110 Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys Gly Asp Gly Ser 115 120 125 Ile Lys Leu Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys 130 135 140 Lys Gly Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser 145 150 155 160 Val Lys <210> 11 <211> 588 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 11 Arg Thr Leu Ala Gly Glu Thr Gly Gln Glu Ala Ala Pro Leu Asp Gly 1 5 10 15 Val Leu Thr Asn Pro Pro Asn Ile Ser Ser Leu Ser Pro Arg Gln Leu 20 25 30 Leu Gly Phe Pro Cys Ala Glu Val Ser Gly Leu Ser Thr Glu Arg Val 35 40 45 Arg Glu Leu Ala Val Ala Leu Ala Gln Lys Asn Val Lys Leu Ser Thr 50 55 60 Glu Gln Leu Arg Cys Leu Ala His Arg Leu Ser Glu Pro Pro Glu Asp 65 70 75 80 Leu Asp Ala Leu Pro Leu Asp Leu Leu Leu Phe Leu Asn Pro Asp Ala 85 90 95 Phe Ser Gly Pro Gln Ala Cys Thr Arg Phe Phe Ser Arg Ile Thr Lys 100 105 110 Ala Asn Val Asp Leu Leu Pro Arg Gly Ala Pro Glu Arg Gln Arg Leu 115 120 125 Leu Pro Ala Ala Leu Ala Cys Trp Gly Val Arg Gly Ser Leu Leu Ser 130 135 140 Glu Ala Asp Val Arg Ala Leu Gly Gly Leu Ala Cys Asp Leu Pro Gly 145 150 155 160 Arg Phe Val Ala Glu Ser Ala Glu Val Leu Leu Pro Arg Leu Val Ser 165 170 175 Cys Pro Gly Pro Leu Asp Gln Asp Gln Gln Glu Ala Ala Arg Ala Ala 180 185 190 Leu Gln Gly Gly Gly Pro Pro Tyr Gly Pro Pro Ser Thr Trp Ser Val 195 200 205 Ser Thr Met Asp Ala Leu Arg Gly Leu Leu Pro Val Leu Gly Gln Pro 210 215 220 Ile Ile Arg Ser Ile Pro Gln Gly Ile Val Ala Ala Trp Arg Gln Arg 225 230 235 240 Ser Ser Arg Asp Pro Ser Trp Arg Gln Pro Glu Arg Thr Ile Leu Arg 245 250 255 Pro Arg Phe Arg Arg Glu Val Glu Lys Thr Ala Cys Pro Ser Gly Lys 260 265 270 Lys Ala Arg Glu Ile Asp Glu Ser Leu Ile Phe Tyr Lys Lys Trp Glu 275 280 285 Leu Glu Ala Cys Val Asp Ala Ala Leu Leu Ala Thr Gln Met Asp Arg 290 295 300 Val Asn Ala Ile Pro Phe Thr Tyr Glu Gln Leu Asp Val Leu Lys His 305 310 315 320 Lys Leu Asp Glu Leu Tyr Pro Gln Gly Tyr Pro Glu Ser Val Ile Gln 325 330 335 His Leu Gly Tyr Leu Phe Leu Lys Met Ser Pro Glu Asp Ile Arg Lys 340 345 350 Trp Asn Val Thr Ser Leu Glu Thr Leu Lys Ala Leu Leu Glu Val Asn 355 360 365 Lys Gly His Glu Met Ser Pro Gln Val Ala Thr Leu Ile Asp Arg Phe 370 375 380 Val Lys Gly Arg Gly Gln Leu Asp Lys Asp Thr Leu Asp Thr Leu Thr 385 390 395 400 Ala Phe Tyr Pro Gly Tyr Leu Cys Ser Leu Ser Pro Glu Glu Leu Ser 405 410 415 Ser Val Pro Pro Ser Ser Ile Trp Ala Val Arg Pro Gln Asp Leu Asp 420 425 430 Thr Cys Asp Pro Arg Gln Leu Asp Val Leu Tyr Pro Lys Ala Arg Leu 435 440 445 Ala Phe Gln Asn Met Asn Gly Ser Glu Tyr Phe Val Lys Ile Gln Ser 450 455 460 Phe Leu Gly Gly Ala Pro Thr Glu Asp Leu Lys Ala Leu Ser Gln Gln 465 470 475 480 Asn Val Ser Met Asp Leu Ala Thr Phe Met Lys Leu Arg Thr Asp Ala 485 490 495 Val Leu Pro Leu Thr Val Ala Glu Val Gln Lys Leu Leu Gly Pro His 500 505 510 Val Glu Gly Leu Lys Ala Glu Glu Arg His Arg Pro Val Arg Asp Trp 515 520 525 Ile Leu Arg Gln Arg Gln Asp Asp Leu Asp Thr Leu Gly Leu Gly Leu 530 535 540 Gln Gly Gly Ile Pro Asn Gly Tyr Leu Val Leu Asp Leu Ser Val Gln 545 550 555 560 Glu Ala Leu Ser Gly Thr Pro Cys Leu Leu Gly Pro Gly Pro Val Leu 565 570 575 Thr Val Leu Ala Leu Leu Leu Ala Ser Thr Leu Ala 580 585 <210> 12 <211> 88 <212> PRT <213> Secuencia artificial <220> <223> fusión <400> 12 Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 1 5 10 15 Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 20 25 30 Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Tyr Glu 35 40 45 Thr Ala Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser 50 55 60 Asn Lys Val Lys Asn Thr Asn Lys Ala Asp Gln Asp Asn Lys Arg Lys 65 70 75 80 Ala Lys Ala Glu Lys Gly Gly Ser 85 <210> 13 <211> 63 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 13 ccagctagtc gtgcattaga ggagaaaaag gggaattacg tggtgacgga tcatggatcg 60 tgt 63 <210> 14 <211> 63 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 14 cctgcatcac gagcacttga agagaaaaaa ggaaactatg ttgtgaccga tcatggtagc 60 tgc 63 <210> 15 <211> 63 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 15 ccagcatcta gagctttaga ggaaaagaag ggtaactatg tcgtaacaga tcatggaagt 60 tgt 63 <210> 16 <211> 63 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 16 ccagcttctc gcgcattaga agaaaagaaa ggcaattatg ttgtaacaga ccatggtagt 60 tgt 63 <210> 17 <211> 63 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 17 ccggcttctc gtgcgctaga agagaagaaa ggaaattacg tagttacaga ccacggctct 60 tgc 63 <210> 18 <211> 231 <212> ADN <213> Secuencia artificial <220> <223> promotor <400> 18 tcctttgatt agtatattcc tatcttaaag ttacttttat gtggaggcat taacatttgt 60 taatgacgtc aaaaggatag caagactaga ataaagctat aaagcaagca tataatattg 120 cgtttcatct ttagaagcga atttcgccaa tattataatt atcaaaagag aggggtggca 180 aacggtattt ggcattatta ggttaaaaaa tgtagaagga gagtgaaacc c 231 <210> 19 <211> 1323 <212> ADN <213> Secuencia artificial <220> <223> Secuencia de LLO441 <400> 19 atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa 60 caaactgaag caaaggatgc atctgcattc aataaagaaa attcaatttc atccatggca 120 ccaccagcat ctccgcctgc aagtcctaag acgccaatcg aaaagaaaca cgcggatgaa 180 atcgataagt atatacaagg attggattac aataaaaaca atgtattagt ataccacgga 240 gatgcagtga caaatgtgcc gccaagaaaa ggttacaaag atggaaatga atatattgtt 300 gtggagaaaa agaagaaatc catcaatcaa aataatgcag acattcaagt tgtgaatgca 360 atttcgagcc taacctatcc aggtgctctc gtaaaagcga attcggaatt agtagaaaat 420 caaccagatg ttctccctgt aaaacgtgat tcattaacac tcagcattga tttgccaggt 480 atgactaatc aagacaataa aatagttgta aaaaatgcca ctaaatcaaa cgttaacaac 540 gcagtaaata cattagtgga aagatggaat gaaaaatatg ctcaagctta tccaaatgta 600 agtgcaaaaa ttgattatga tgacgaaatg gcttacagtg aatcacaatt aattgcgaaa 660 tttggtacag catttaaagc tgtaaataat agcttgaatg taaacttcgg cgcaatcagt 720 gaagggaaaa tgcaagaaga agtcattagt tttaaacaaa tttactataa cgtgaatgtt 780 aatgaaccta caagaccttc cagatttttc ggcaaagctg ttactaaaga gcagttgcaa 840 gcgcttggag tgaatgcaga aaatcctcct gcatatatct caagtgtggc gtatggccgt 900 caagtttatt tgaaattatc aactaattcc catagtacta aagtaaaagc tgcttttgat 960 gctgccgtaa gcggaaaatc tgtctcaggt gatgtagaac taacaaatat catcaaaaat 1020 tcttccttca aagccgtaat ttacggaggt tccgcaaaag atgaagttca aatcatcgac 1080 ggcaacctcg gagacttacg cgatattttg aaaaaaggcg ctacttttaa tcgagaaaca 1140 ccaggagttc ccattgctta tacaacaaac ttcctaaaag acaatgaatt agctgttatt 1200 aaaaacaact cagaatatat tgaaacaact tcaaaagctt atacagatgg aaaaattaac 1260 atcgatcact ctggaggata cgttgctcaa ttcaacattt cttgggatga agtaaattat 1320 gat 1323 <210> 20 <211> 441 <212> PRT <213> Secuencia artificial <220> <223> Secuencia de LLO441 <400> 20 Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 1 5 10 15 Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 20 25 30 Glu Asn Ser Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 35 40 45 Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 50 55 60 Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 65 70 75 80 Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 85 90 95 Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 100 105 110 Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 115 120 125 Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 130 135 140 Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 145 150 155 160 Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 165 170 175 Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 180 185 190 Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 195 200 205 Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 210 215 220 Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 225 230 235 240 Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 245 250 255 Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 260 265 270 Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 275 280 285 Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 290 295 300 Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 305 310 315 320 Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 325 330 335 Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 340 345 350 Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 355 360 365 Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 370 375 380 Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 385 390 395 400 Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 405 410 415 Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 420 425 430 Ile Ser Trp Asp Glu Val Asn Tyr Asp 435 440 <210> 21 <211> 1266 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 21 atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa 60 caaactgaag caaaggatgc atctgcattc aatacgccaa tcgaaaagaa acacgcggat 120 gaaatcgata agtatataca aggattggat tacaataaaa acaatgtatt agtataccac 180 ggagatgcag tgacaaatgt gccgccaaga aaaggttaca aagatggaaa tgaatatatt 240 gttgtggaga aaaagaagaa atccatcaat caaaataatg cagacattca agttgtgaat 300 gcaatttcga gcctaaccta tccaggtgct ctcgtaaaag cgaattcgga attagtagaa 360 aatcaaccag atgttctccc tgtaaaacgt gattcattaa cactcagcat tgatttgcca 420 ggtatgacta atcaagacaa taaaatagtt gtaaaaaatg ccactaaatc aaacgttaac 480 aacgcagtaa atacattagt ggaaagatgg aatgaaaaat atgctcaagc ttatccaaat 540 gtaagtgcaa aaattgatta tgatgacgaa atggcttaca gtgaatcaca attaattgcg 600 aaatttggta cagcatttaa agctgtaaat aatagcttga atgtaaactt cggcgcaatc 660 agtgaaggga aaatgcaaga agaagtcatt agttttaaac aaatttacta taacgtgaat 720 gttaatgaac ctacaagacc ttccagattt ttcggcaaag ctgttactaa agagcagttg 780 caagcgcttg gagtgaatgc agaaaatcct cctgcatata tctcaagtgt ggcgtatggc 840 cgtcaagttt atttgaaatt atcaactaat tcccatagta ctaaagtaaa agctgctttt 900 gatgctgccg taagcggaaa atctgtctca ggtgatgtag aactaacaaa tatcatcaaa 960 aattcttcct tcaaagccgt aatttacgga ggttccgcaa aagatgaagt tcaaatcatc 1020 gacggcaacc tcggagactt acgcgatatt ttgaaaaaag gcgctacttt taatcgagaa 1080 acaccaggag ttcccattgc ttatacaaca aacttcctaa aagacaatga attagctgtt 1140 attaaaaaca actcagaata tattgaaaca acttcaaaag cttatacaga tggaaaaatt 1200 aacatcgatc actctggagg atacgttgct caattcaaca tttcttggga tgaagtaaat 1260 tatgat 1266 <210> 22 <211> 422 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 22 Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 1 5 10 15 Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Thr 20 25 30 Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr Ile Gln Gly 35 40 45 Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly Asp Ala Val 50 55 60 Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn Glu Tyr Ile 65 70 75 80 Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn Ala Asp Ile 85 90 95 Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly Ala Leu Val 100 105 110 Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val Leu Pro Val 115 120 125 Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly Met Thr Asn 130 135 140 Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser Asn Val Asn 145 150 155 160 Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys Tyr Ala Gln 165 170 175 Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp Glu Met Ala 180 185 190 Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala Phe Lys Ala 195 200 205 Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser Glu Gly Lys 210 215 220 Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr Asn Val Asn 225 230 235 240 Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys Ala Val Thr 245 250 255 Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn Pro Pro Ala 260 265 270 Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu Lys Leu Ser 275 280 285 Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp Ala Ala Val 290 295 300 Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn Ile Ile Lys 305 310 315 320 Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala Lys Asp Glu 325 330 335 Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp Ile Leu Lys 340 345 350 Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro Ile Ala Tyr 355 360 365 Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile Lys Asn Asn 370 375 380 Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp Gly Lys Ile 385 390 395 400 Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn Ile Ser Trp 405 410 415 Asp Glu Val Asn Tyr Asp 420 <210> 23 <211> 1245 <212> ADN <213> Secuencia artificial <220> <223> fragmento <400> 23 atgaaaaaaa taatgctagt ttttattaca cttatattag ttagtctacc aattgcgcaa 60 caaactgaag caaaggatgc atctgcattc aataaagaag aaatcgataa gtatatacaa 120 ggattggatt acaataaaaa caatgtatta gtataccacg gagatgcagt gacaaatgtg 180 ccgccaagaa aaggttacaa agatggaaat gaatatattg ttgtggagaa aaagaagaaa 240 tccatcaatc aaaataatgc agacattcaa gttgtgaatg caatttcgag cctaacctat 300 ccaggtgctc tcgtaaaagc gaattcggaa ttagtagaaa atcaaccaga tgttctccct 360 gtaaaacgtg attcattaac actcagcatt gatttgccag gtatgactaa tcaagacaat 420 aaaatagttg taaaaaatgc cactaaatca aacgttaaca acgcagtaaa tacattagtg 480 gaaagatgga atgaaaaata tgctcaagct tatccaaatg taagtgcaaa aattgattat 540 gatgacgaaa tggcttacag tgaatcacaa ttaattgcga aatttggtac agcatttaaa 600 gctgtaaata atagcttgaa tgtaaacttc ggcgcaatca gtgaagggaa aatgcaagaa 660 gaagtcatta gttttaaaca aatttactat aacgtgaatg ttaatgaacc tacaagacct 720 tccagatttt tcggcaaagc tgttactaaa gagcagttgc aagcgcttgg agtgaatgca 780 gaaaatcctc ctgcatatat ctcaagtgtg gcgtatggcc gtcaagttta tttgaaatta 840 tcaactaatt cccatagtac taaagtaaaa gctgcttttg atgctgccgt aagcggaaaa 900 tctgtctcag gtgatgtaga actaacaaat atcatcaaaa attcttcctt caaagccgta 960 atttacggag gttccgcaaa agatgaagtt caaatcatcg acggcaacct cggagactta 1020 cgcgatattt tgaaaaaagg cgctactttt aatcgagaaa caccaggagt tcccattgct 1080 tatacaacaa acttcctaaa agacaatgaa ttagctgtta ttaaaaacaa ctcagaatat 1140 attgaaacaa cttcaaaagc ttatacagat ggaaaaatta acatcgatca ctctggagga 1200 tacgttgctc aattcaacat ttcttgggat gaagtaaatt atgat 1245 <210> 24 <211> 415 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 24 Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 1 5 10 15 Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe Asn Lys 20 25 30 Glu Glu Ile Asp Lys Tyr Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn 35 40 45 Val Leu Val Tyr His Gly Asp Ala Val Thr Asn Val Pro Pro Arg Lys 50 55 60 Gly Tyr Lys Asp Gly Asn Glu Tyr Ile Val Val Glu Lys Lys Lys Lys 65 70 75 80 Ser Ile Asn Gln Asn Asn Ala Asp Ile Gln Val Val Asn Ala Ile Ser 85 90 95 Ser Leu Thr Tyr Pro Gly Ala Leu Val Lys Ala Asn Ser Glu Leu Val 100 105 110 Glu Asn Gln Pro Asp Val Leu Pro Val Lys Arg Asp Ser Leu Thr Leu 115 120 125 Ser Ile Asp Leu Pro Gly Met Thr Asn Gln Asp Asn Lys Ile Val Val 130 135 140 Lys Asn Ala Thr Lys Ser Asn Val Asn Asn Ala Val Asn Thr Leu Val 145 150 155 160 Glu Arg Trp Asn Glu Lys Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala 165 170 175 Lys Ile Asp Tyr Asp Asp Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile 180 185 190 Ala Lys Phe Gly Thr Ala Phe Lys Ala Val Asn Asn Ser Leu Asn Val 195 200 205 Asn Phe Gly Ala Ile Ser Glu Gly Lys Met Gln Glu Glu Val Ile Ser 210 215 220 Phe Lys Gln Ile Tyr Tyr Asn Val Asn Val Asn Glu Pro Thr Arg Pro 225 230 235 240 Ser Arg Phe Phe Gly Lys Ala Val Thr Lys Glu Gln Leu Gln Ala Leu 245 250 255 Gly Val Asn Ala Glu Asn Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr 260 265 270 Gly Arg Gln Val Tyr Leu Lys Leu Ser Thr Asn Ser His Ser Thr Lys 275 280 285 Val Lys Ala Ala Phe Asp Ala Ala Val Ser Gly Lys Ser Val Ser Gly 290 295 300 Asp Val Glu Leu Thr Asn Ile Ile Lys Asn Ser Ser Phe Lys Ala Val 305 310 315 320 Ile Tyr Gly Gly Ser Ala Lys Asp Glu Val Gln Ile Ile Asp Gly Asn 325 330 335 Leu Gly Asp Leu Arg Asp Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg 340 345 350 Glu Thr Pro Gly Val Pro Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp 355 360 365 Asn Glu Leu Ala Val Ile Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr 370 375 380 Ser Lys Ala Tyr Thr Asp Gly Lys Ile Asn Ile Asp His Ser Gly Gly 385 390 395 400 Tyr Val Ala Gln Phe Asn Ile Ser Trp Asp Glu Val Asn Tyr Asp 405 410 415 <210> 25 <211> 20 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 25 Pro Ala Ser Arg Leu Glu Glu Lys Lys Gly Asn Tyr Val Val Thr Asp 1 5 10 15 His Gly Ser Cys 20 <210> 26 <211> 34 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 26 Ala Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser Val Lys 20 25 30 Thr Ser <210> 27 <211> 32 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 27 Ala Ser Lys Val Ala Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Gly Asp Gly Ser Ile Lys 20 25 30 <210> 28 <211> 32 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 28 Leu Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser Val Lys 20 25 30 <210> 29 <211> 32 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 29 Ala Ser Lys Val Ala Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Gly Asp Gly Ser Ile Lys 20 25 30 <210> 30 <211> 32 <212> PRT <213> Secuencia artificial <220> <223> fragmento <400> 30 Leu Ser Lys Val Leu Pro Ala Ser Arg Ala Leu Glu Glu Lys Lys Gly 1 5 10 15 Asn Tyr Val Val Thr Asp His Gly Ser Cys Ala Asp Gly Ser Val Lys 20 25 30 <210> 31 <211> 300 <212> ADN <213> Secuencia artificial <220> <223> ActAN100 <400> 31 gtgggattaa atagatttat gcgtgcgatg atggtagttt tcattactgc caactgcatt 60 acgattaacc ccgacataat atttgcagcg acagatagcg aagattccag tctaaacaca 120 gatgaatggg aagaagaaaa aacagaagag cagccaagcg aggtaaatac gggaccaaga 180 tacgaaactg cacgtgaagt aagttcacgt gatattgagg aactagaaaa atcgaataaa 240 gtgaaaaata cgaacaaagc agacctaata gcaatgttga aagcaaaagc agagaaaggt 300 <210> 32 <211> 100 <212> PRT <213> Secuencia artificial <220> <223> ActAN100 <400> 32 Met Gly Leu Asn Arg Phe Met Arg Ala Met Met Val Val Phe Ile Thr 1 5 10 15 Ala Asn Cys Ile Thr Ile Asn Pro Asp Ile Ile Phe Ala Ala Thr Asp 20 25 30 Ser Glu Asp Ser Ser Leu Asn Thr Asp Glu Trp Glu Glu Glu Lys Thr 35 40 45 Glu Glu Gln Pro Ser Glu Val Asn Thr Gly Pro Arg Tyr Glu Thr Ala 50 55 60 Arg Glu Val Ser Ser Arg Asp Ile Glu Glu Leu Glu Lys Ser Asn Lys 65 70 75 80 Val Lys Asn Thr Asn Lys Ala Asp Leu Ile Ala Met Leu Lys Ala Lys 85 90 95 Ala Glu Lys Gly 100