LISTADO DE SECUENCIAS <110> Sanofi <120> Moléculas de fusión <130> DE2015/080WOPCT <150> EP 15 306 106.4 <151> 7 de julio, 2015 <160> 186 <170> PatentIn versión 3.5 <210> 1 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> NGAL de tipo salvaje <400> 1 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 65 70 75 80 Arg Thr Phe Val Pro Gly Cys Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 115 120 125 Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 2 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.12C05 <400> 2 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Asn Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ile Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Val Val Gln Phe Trp Asp Lys Lys Cys Leu Tyr Gln Ile 65 70 75 80 Gln Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly His 85 90 95 Ile Lys Ser Lys Pro Gly His Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Arg Val Trp Gln 115 120 125 Asn Arg Glu Trp Phe Asp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 3 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.02P05 <400> 3 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Thr Ala Gly Asn Gly Phe Leu Arg Glu Asp Lys Asp Pro 35 40 45 Leu Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Ser Val Trp Phe Ala Leu Lys Lys Cys Tyr Tyr Asp Ile 65 70 75 80 Gly Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ile 85 90 95 Ile Lys Ser Glu Pro Gly His Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val Asn Gln 115 120 125 Asn Arg Glu Asn Phe Gln Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 4 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.09A08 <400> 4 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Ser Glu Lys Lys Cys Ser Tyr Ser Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Asn Pro Gly Lys Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Ala Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 5 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.09M05 <400> 5 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Phe Ala Gly Asn Asn Arg Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Thr Phe Glu Ala Lys Lys Cys Arg Tyr Arg Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Tyr 85 90 95 Ile Lys Ser Lys Pro Gly Pro Thr Ser Phe Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ser Val Thr Gln 115 120 125 Asn Arg Glu Trp Phe Gly Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 6 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.09E05 <400> 6 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Val Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Val Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Phe Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln 115 120 125 Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 7 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.09E13 <400> 7 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Phe Phe Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ala Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Trp Phe Leu Asn Lys Lys Cys Gln Tyr Glu Ile 65 70 75 80 His Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Tyr 85 90 95 Ile Lys Ser Tyr Pro Gly Tyr Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Val Val His Gln 115 120 125 Asn Arg Asp Lys Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 8 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.17L07 <400> 8 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Pro Glu Lys Lys Cys Ile Tyr Ser Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Ser Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 9 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.17O10 <400> 9 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Pro Asp Lys Lys Cys Ile Tyr Ser Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Asn Pro Gly Asp Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Val Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 10 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.19M08 <400> 10 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Pro Glu Lys Lys Cys Thr Tyr Ser Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Asn Pro Gly Glu Thr Ser His Leu Val Arg Val Met Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Asp Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 11 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.17O03 <400> 11 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Pro Asp Lys Lys Cys Val Tyr Ser Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Asn Pro Gly Asn Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Val Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 12 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0510.05H10 <400> 12 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Leu Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Val Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln 115 120 125 Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 13 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.06H10 <400> 13 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Ser Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 14 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.04N16 <400> 14 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro 35 40 45 Leu Lys Met Met Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Val Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr 65 70 75 80 Glu Thr Phe Val Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln 115 120 125 Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 15 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0544.01B06_H28Q_N65D <400> 15 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asp Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Ser Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 16 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0544.04C04_H28Q_N65D <400> 16 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Trp Ala Gly Asn Thr Ala Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Pro Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Ser Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln 115 120 125 Asn Arg Glu Gly Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 17 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.04N16_N65D <400> 17 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro 35 40 45 Leu Lys Met Met Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Val Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr 65 70 75 80 Glu Thr Phe Val Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln 115 120 125 Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 18 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.04N16_N65Q <400> 18 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro 35 40 45 Leu Lys Met Met Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Val Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr 65 70 75 80 Glu Thr Phe Val Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln 115 120 125 Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 19 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S447.3 M1.1_N4 <400> 19 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Glu Val Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Ser Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Asn Pro Gly Gln Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 20 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S449.1 M1.1_M5 <400> 20 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Arg Phe Ile Met Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ile 85 90 95 Ile Lys Ser Asn Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Met Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 21 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S449.5 M1.1_L10 <400> 21 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Ile Ala Gly Asn Thr Val Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Ala Pro Lys Lys Cys Ile Tyr Ser Ile 65 70 75 80 Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Met 85 90 95 Ile Lys Ser Ser Pro Gly Gly Thr Ser Ala Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 115 120 125 Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 22 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S449.8 M1.1_F3 <400> 22 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Asn Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ala Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Ser Gln Lys Lys Cys Met Tyr Ala Ile 65 70 75 80 Tyr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 85 90 95 Ile Lys Ser Pro Pro Gly Thr Thr Ser Ile Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Met Gln 115 120 125 Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 23 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S449.5 M1.1_E8 <400> 23 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn His Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ala Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Gly Arg Lys Lys Cys His Tyr Trp Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 85 90 95 Ile Lys Ser Asp Pro Gly Met Thr Ser Phe Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ala Val Asp Gln 115 120 125 Asn Arg Glu Asn Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 24 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S437.1 M1.1_P2 <400> 24 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Asn Ala Gly Asn Gly Arg Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Arg Val Trp Phe Asn Gln Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp 85 90 95 Ile Lys Ser Thr Pro Gly Trp Thr Ser Asn Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Asn Val Met Gln 115 120 125 Asn Arg Glu Ile Phe Tyr Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 25 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S449.1 M1.1_I7 <400> 25 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Gly Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Gly Arg Lys Lys Cys Gly Tyr Trp Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 85 90 95 Ile Lys Ser Trp Pro Gly Ile Thr Ser Gly Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Asn Gln 115 120 125 Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 26 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.06L06 <400> 26 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Glu Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Ser Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 85 90 95 Ile Lys Ser Asn Pro Gly Val Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Asn Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 27 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.08G23 <400> 27 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Ser Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Gln Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Lys Gln 115 120 125 Asn Arg Glu Val Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 28 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.06G17 <400> 28 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe His Lys Lys Lys Cys Asn Tyr Phe Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 85 90 95 Ile Lys Ser His Pro Gly Gln Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Lys Gln 115 120 125 Asn Arg Glu Ala Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 29 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.03N20 <400> 29 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Gln Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Trp Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Asn Pro Gly His Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 30 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.05F20 <400> 30 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Arg Phe Ile Phe Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Met Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 31 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22 <400> 31 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 32 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.06L06_N65D_S79F <400> 32 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Glu Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Phe Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 85 90 95 Ile Lys Ser Asn Pro Gly Val Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Asn Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 33 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0475.06L06_N65Q_S79F <400> 33 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Glu Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Phe Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 85 90 95 Ile Lys Ser Asn Pro Gly Val Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Asn Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 34 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65D <400> 34 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 35 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65Q <400> 35 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 36 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_H28Q_T54A_N65D <400> 36 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 37 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_N65Q <400> 37 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 38 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.14D07 <400> 38 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Phe Ala Gly Asn Trp Met Leu Arg Glu Asp Lys Asp Pro 35 40 45 His Lys Met Asn Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Lys Phe Gln Ala Lys Lys Cys Ile Tyr Ser Ile 65 70 75 80 His Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ile 85 90 95 Ile Lys Ser Asn Pro Gly Gly Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val His Gln 115 120 125 Asn Arg Glu Phe Phe Gln Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 39 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.05N20 <400> 39 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Phe Ala Gly Asn Arg Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ile Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Gln Val Asn Phe Trp Leu Lys Lys Cys Ala Tyr Ser Ile 65 70 75 80 Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Arg 85 90 95 Ile Lys Ser Ile Pro Gly Pro Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Thr Val Ile Gln 115 120 125 Asn Arg Glu Phe Phe Glu Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 40 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.16B14 <400> 40 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Phe Ala Gly Asn Leu Leu Leu Arg Glu Asp Lys Asp Pro 35 40 45 Arg Lys Met Arg Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Arg Phe Leu Tyr Lys Lys Cys Ile Tyr Ser Ile 65 70 75 80 Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly 85 90 95 Ile Lys Ser Ala Pro Gly Phe Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val Ala Gln 115 120 125 Asn Arg Glu Tyr Phe Glu Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 41 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.15E20 <400> 41 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Phe Ala Gly Asn Trp Arg Leu Arg Glu Asp Lys Asp Pro 35 40 45 Pro Lys Met Ser Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asn Val Arg Phe Trp Pro Lys Lys Cys Arg Tyr Ser Ile 65 70 75 80 Ser Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Met 85 90 95 Ile Lys Ser Pro Pro Gly Gly Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val Phe Gln 115 120 125 Asn Arg Glu Phe Phe Glu Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 42 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0466.13C10 <400> 42 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 35 40 45 Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr Asp Ile 65 70 75 80 Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 43 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0510.11H24 <400> 43 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 44 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.01M12_x103Q <400> 44 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Thr Leu Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg Arg Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Val 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 45 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0512.01F10_x103Q <400> 45 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 46 <211> 174 <212> PRT <213> Secuencia Artificial <220> <223> S0510.10A04_x103Q <400> 46 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 35 40 45 Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg Tyr Lys Lys Cys Gln Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Ser Glu 85 90 95 Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn 100 105 110 Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Phe 115 120 125 Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 130 135 140 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 145 150 155 160 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 165 170 <210> 47 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07G09 <400> 47 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Arg Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 48 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07K20 <400> 48 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asn Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Pro Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 49 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07K21 <400> 49 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Gly Arg Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 50 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07G09_N65D <400> 50 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Arg Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 51 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07K20_N65D <400> 51 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asn Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Pro Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 52 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07K21_N65D <400> 52 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Gly Arg Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 53 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0530.07G09_H28Q_N65D <400> 53 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Glu Ala Gly Asn Leu Ala Arg Arg Glu Asp Lys Asp Pro 35 40 45 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 65 70 75 80 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 85 90 95 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 115 120 125 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 54 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0455.04F12 <400> 54 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Ile Leu Leu Arg Glu Asp Lys Asp Pro 35 40 45 His Lys Met Leu Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr His Val Thr Phe Lys Trp Lys Lys Cys Tyr Tyr Ala Ile 65 70 75 80 Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Met 85 90 95 Ile Lys Ser Glu Pro Gly His Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Trp Val Asp Gln 115 120 125 Asn Arg Glu Glu Phe Leu Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 55 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0455.04F23 <400> 55 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys His Tyr Arg Ile 65 70 75 80 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 115 120 125 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 56 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0455.04F09 <400> 56 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 85 90 95 Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln 115 120 125 Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 57 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0455.04M08 <400> 57 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Tyr Trp Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ala Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Arg Phe Trp Arg Lys Lys Cys Arg Tyr Asp Ile 65 70 75 80 Trp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Pro 85 90 95 Ile Lys Ser Glu Pro Gly Gln Thr Ser Arg Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Leu Val Arg Gln 115 120 125 Asn Arg Glu Ala Phe Asn Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 58 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0522.15K24 <400> 58 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Leu Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 85 90 95 Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys Gly Val Trp Gln 115 120 125 Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Ala Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 59 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0527.05N11 <400> 59 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro 35 40 45 Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Thr 65 70 75 80 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 85 90 95 Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys Gly Val Trp Gln 115 120 125 Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 60 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0522.05A05 <400> 60 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Gly Arg Asp Pro 35 40 45 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys Leu Tyr Arg Ile 65 70 75 80 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 115 120 125 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 61 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0522.05M06 <400> 61 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly Asp Tyr Asp Pro 35 40 45 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys His Tyr Arg Ile 65 70 75 80 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe Gly Asn 85 90 95 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 115 120 125 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 62 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0522.05A05_H28Q_N65D <400> 62 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Gly Arg Asp Pro 35 40 45 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys Leu Tyr Arg Ile 65 70 75 80 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 115 120 125 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 63 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> S0522.05M06_N65D <400> 63 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly Asp Tyr Asp Pro 35 40 45 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys His Tyr Arg Ile 65 70 75 80 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe Gly Asn 85 90 95 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 115 120 125 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 64 <211> 178 <212> PRT <213> Secuencia Artificial <220> <223> 3004wt = NGAL98 <400> 64 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Leu Ala Gly Asn Ala Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gln Lys Met Tyr Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asn Val Thr Ser Val Leu Phe Arg Lys Lys Lys Cys Asp Tyr Trp Ile 65 70 75 80 Arg Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Leu Thr Ser Tyr Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Ser Gln 115 120 125 Asn Arg Glu Tyr Phe Lys Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly <210> 65 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.12C05 <400> 65 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcaatgc cggaaatgga 120 tggctgcgtg aggataagga tccgatcaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgttgtgcaa ttttgggaca agaaatgcct gtaccaaatt 240 caaacctttg tgccggggag ccagccgggc gagtttactt taggccacat taaaagtaaa 300 ccgggccaca catcacactt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agcgtgtgtg gcagaaccgc gagtggtttg acatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 66 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.02P05 <400> 66 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcaccgc cggaaatgga 120 ttcctgcgtg aggataagga tccgctgaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cagcgtgtgg tttgcactga agaaatgcta ctacgacatt 240 ggaacctttg tgccggggag ccagccgggc gagtttactt taggcatcat taaaagtgag 300 ccgggccaca catcacaatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtgggtgaa tcagaaccgc gagaattttc aaatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 67 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.09A08 <400> 67 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatacc 120 accctgcgtg aggataagga tccgcctaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtgcaa tttagcgaga agaaatgcag ctacagcatt 240 atcacctttg tgccggggag ccagccgggc gagtttactt taggcggaat taaaagtaat 300 ccgggcaaaa catcacactt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtacgtggc acagaaccgc gagggattta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 68 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.09M05 <400> 68 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcttcgc cggaaataat 120 cgtctgcgtg aggataagga tccgcctaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtgacc tttgaggcaa agaaatgccg ttaccgtatt 240 atcacctttg tgccggggag ccagccgggc gagtttactt taggctacat taaaagtaaa 300 ccgggcccta catcattctt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agagcgtgac ccagaaccgc gagtggtttg gaatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 69 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.09E05 <400> 69 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcctggc cggaaatgga 120 tggctgcgtg aggataagga tccggttaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgttgtggac tttgagctga agaaatgccg ttacatgatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcgacat taaaagtttc 300 ccgggctgga catcacaatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggagtgta ccagaaccgc gagtggtttc acatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 70 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.09E13 <400> 70 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatttc 120 ttcctgcgtg aggataagga tccggcaaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgtgg tttctgaata agaaatgcca atacgagatt 240 cacacctttg tgccggggag ccagccgggc gagtttactt taggctacat taaaagttac 300 ccgggctaca catcacactt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca aggttgtgca ccagaaccgc gataaatttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 71 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.17L07 <400> 71 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatacg 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgcag tttcctgaga agaaatgcat ttactctact 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcggtat taaaagtagt 300 ccgggccaga catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtgat tcagaaccgc gaggggttta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 72 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.17O10 <400> 72 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatacg 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgcag tttccggata agaaatgcat ttactcgatt 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcgggat taaaagtaat 300 ccgggcgata catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtggt gcagaaccgc gagggtttta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 73 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.19M08 <400> 73 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatacg 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgcag tttcctgaga agaaatgcac gtactcgatt 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcgatat taaaagtaat 300 ccgggcgaga catcacattt ggtccgcgtc atgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtgga tcagaaccgc gaggggttta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 74 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.17O03 <400> 74 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatact 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgcag tttccggata agaaatgcgt gtactcgatt 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcgggat taaaagtaat 300 ccgggcaata catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtggt gcagaaccgc gaggggttta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 75 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0510.05H10 <400> 75 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccttgc cggaaatggt 120 tggctgcgtg aggataagga tccgcttaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgtggtggat tttgagctta agaaatgcag gtacatgatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcgatat taaaagttct 300 ccgggctgga catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggggtgta tcagaaccgc gagtggtttc atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 76 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.06H10 <400> 76 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggctgggc cggaaatacg 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcgg tcatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgcag tttcccgaga aggaatgcat ttactctact 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcggtat taaaagtagt 300 ccgggccaga catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtgac tcagaaccgc gaggggttta atatcacgct gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 77 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.04N16 <400> 77 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccttgc cggaaatggt 120 tggctgcgtg aggatgagga tccgcttaaa atgatggcgg ccgtttacga gttgagagaa 180 gataaatcat ataacgtcac cgtggtggat tttgagcttg aggaatgcag gtacatgact 240 gagacctttg tgccggggaa ccagccgggc gagtttactt taggcgatat taaaagttct 300 ccgggctgga catcacagct ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggggtgta tcagaaccgc gagtggtttc atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 78 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0544.01B06_H28Q_N65D <400> 78 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggctgggc cggagatacg 120 acgctgcgtg aggataagga tccgcctaaa atgcctgcgg tcatttacga gttgaaagaa 180 gataaatcat atgatgtcac cgatgtgcag tttcccgaga aggaatgcat ttactctact 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcggtat taaaagtagt 300 ccgggccaga catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtgac tcagaaccgc gaggggttta atatcgcgct gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 79 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0544.04C04_H28Q_N65D <400> 79 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggctgggc cggaaatacg 120 gctctgcgtg aggataagga tccgcctaaa atgcctgcgg tcatttacga gttgaaagaa 180 gataaatcat atgatgtcac cgatgtgcag tttcccgaga aggaatgcat ttactctact 240 attacctttg tgccggggag ccagccgggc gagtttactt taggcggtat taaaagtagt 300 ccgggccaga catcacattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtatgtgac tcagaaccgc gaggggttta atatcgctct gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 80 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.04N16_N65D <400> 80 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccttgc cggaaatggt 120 tggctgcgtg aggatgagga tccgcttaaa atgatggcgg ccgtttacga gttgagagaa 180 gataaatcat atgacgtcac cgtggtggat tttgagcttg aggaatgcag gtacatgact 240 gagacctttg tgccggggaa ccagccgggc gagtttactt taggcgatat taaaagttct 300 ccgggctgga catcacagct ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggggtgta tcagaaccgc gagtggtttc atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 81 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.04N16_N65Q <400> 81 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccttgc cggaaatggt 120 tggctgcgtg aggatgagga tccgcttaaa atgatggcgg ccgtttacga gttgagagaa 180 gataaatcat atcaggtcac cgtggtggat tttgagcttg aggaatgcag gtacatgact 240 gagacctttg tgccggggaa ccagccgggc gagtttactt taggcgatat taaaagttct 300 ccgggctgga catcacagct ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggggtgta tcagaaccgc gagtggtttc atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 82 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S447.3 M1.1_N4 <400> 82 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatgag 120 gttctgcgtg aggataagga tccgggaaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttcacaata agaaatgcaa ttacagcatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagtaat 300 ccgggccaaa catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaagtgaa acagaaccgc gagggatttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 83 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S449.1 M1.1_M5 <400> 83 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcctggc cggaaatacc 120 atcctgcgtg aggataagga tccgggaaaa atgaatgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtgcgt tttatcatga agaaatgcca ctactacatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcatcat taaaagtaat 300 ccgggcacca catcacaatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agatcgtgcg tcagaaccgc gagatgtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 84 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S449.5 M1.1_L10 <400> 84 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcatcgc cggaaatacc 120 gttctgcgtg aggataagga tccgggaaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttgcaccta agaaatgcat ctacagcatt 240 agcacctttg tgccggggag ccagccgggc gagtttactt taggcatgat taaaagtagc 300 ccgggcggaa catcagcatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaagtgag ccagaaccgc gaggtttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 85 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S449.8 M1.1_F3 <400> 85 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcctggc cggaaataat 120 atcctgcgtg aggataagga tccggcaaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttagccaaa agaaatgcat gtacgcaatt 240 tacacctttg tgccggggag ccagccgggc gagtttactt taggccgtat taaaagtcct 300 ccgggcacca catcaatctt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaagtgat gcagaaccgc gagttctttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 86 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S449.5 M1.1_E8 <400> 86 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcctggc cggaaatcac 120 atcctgcgtg aggataagga tccggcaaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttggacgta agaaatgcca ctactggatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggccgtat taaaagtgac 300 ccgggcatga catcattctt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca aggcagtgga ccagaaccgc gagaattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 87 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S437.1 M1.1_P2 <400> 87 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcaatgc cggaaatgga 120 cgtctgcgtg aggataagga tccgcctaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccgtgtgtgg tttaatcaaa agaaatgcaa atacgacatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcgacat taaaagtacc 300 ccgggctgga catcaaattt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaatgtgat gcagaaccgc gagatctttt acatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 88 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S449.1 M1.1_I7 <400> 88 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatacc 120 accctgcgtg aggataagga tccgggaaaa atgggagcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttggacgta agaaatgcgg atactggatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcgcaat taaaagttgg 300 ccgggcatca catcaggatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaagtgaa tcagaaccgc gaggtttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 89 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.06L06 <400> 89 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatgag 120 gtgctgcgtg atgataagga tccggggaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttcataata agaaatgcaa ttactcgatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtaat 300 ccgggcgtga catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaatgtgaa gcagaaccgc gaggggtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 90 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.08G23 <400> 90 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatact 120 gtgctgcgtg atgataagga tccggggaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttcataata agaaatgcaa ttactctatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggccaga catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtgaa gcagaaccgc gaggtgtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 91 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.06G17 <400> 91 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatacg 120 gtgctgcgtg acgataagga tccgggtaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgg tttcataaga agaaatgcaa ttactttatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtcat 300 ccgggccaga catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtgaa gcagaaccgc gaggcgtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 92 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.03N20 <400> 92 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatcag 120 gtgctgcgtg atgataagga tccgggtaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttcataata agaaatgcaa ttactggatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagtaat 300 ccgggccata catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtgaa gcagaaccgc gagggttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 93 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.05F20 <400> 93 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaatacg 120 attctgcgtg aggataagga tccggggaaa atgaatgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgagg tttattttta agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcatga catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 94 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.01H22 <400> 94 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatact 120 attctgcgtg aggataagga tccggggaaa atgaatgcaa ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgatgtgagg tttattagga agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcacta catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 95 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.06L06_N65D_S79F <400> 95 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatgag 120 gtgctgcgtg atgataagga tccggggaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat atgatgtcac cgaggtgagg tttcataata agaaatgcaa ttacttcatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtaat 300 ccgggcgtga catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaatgtgaa gcagaaccgc gaggggtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 96 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0475.06L06_N65Q_S79F <400> 96 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatgag 120 gtgctgcgtg atgataagga tccggggaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat atcaggtcac cgaggtgagg tttcataata agaaatgcaa ttacttcatt 240 gagacctttg tgccggggag ccagccgggc gagtttactt taggcaagat taaaagtaat 300 ccgggcgtga catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaatgtgaa gcagaaccgc gaggggtttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 97 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65D <400> 97 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatact 120 attctgcgtg aggataagga tccggggaaa atgaatgcag ccatttacga gttgaaagaa 180 gataaatcat atgatgtcac cgatgtgagg tttattagga agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcacta catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 98 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65Q <400> 98 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatact 120 attctgcgtg aggataagga tccggggaaa atgaatgcag ccatttacga gttgaaagaa 180 gataaatcat atcaggtcac cgatgtgagg tttattagga agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcacta catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 99 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.01H22_H28Q_T54A_N65D <400> 99 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcgtggc cggaaatact 120 attctgcgtg aggataagga tccggggaaa atgaatgcag ccatttacga gttgaaagaa 180 gataaatcat atgatgtcac cgatgtgagg tttattagga agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcacta catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 100 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0474.01H22_N65Q <400> 100 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgtggc cggaaatact 120 attctgcgtg aggataagga tccggggaaa atgaatgcaa ccatttacga gttgaaagaa 180 gataaatcat atcaggtcac cgatgtgagg tttattagga agaaatgcca ttactatatt 240 gatacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagttat 300 ccgggcacta catcacagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agattgtgag gcagaaccgc gagatttttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 101 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.14D07 <400> 101 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcttcgc cggaaattgg 120 atgctgcgtg aggataagga tccgcacaaa atgaatgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgaaa tttcaagcaa agaaatgcat ctacagcatt 240 cacacctttg tgccggggag ccagccgggc gagtttactt taggcatcat taaaagtaat 300 ccgggcggaa catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtgggtgca ccagaaccgc gagttctttc aaatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 102 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.05N20 <400> 102 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcttcgc cggaaatcgt 120 tggctgcgtg aggataagga tccgatcaaa atgtacgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccaagtgaat ttttggctga agaaatgcgc atacagcatt 240 agcacctttg tgccggggag ccagccgggc gagtttactt taggccgtat taaaagtatc 300 ccgggcccta catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaccgtgat ccagaaccgc gagttctttg agatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 103 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.16B14 <400> 103 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcttcgc cggaaatctg 120 ctgctgcgtg aggataagga tccgcgtaaa atgcgtgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtgcgt tttctgtaca agaaatgcat ctacagcatt 240 gcaacctttg tgccggggag ccagccgggc gagtttactt taggcggaat taaaagtgca 300 ccgggcttca catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtgggtggc acagaaccgc gagtactttg agatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 104 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.15E20 <400> 104 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcttcgc cggaaattgg 120 cgtctgcgtg aggataagga tccgcctaaa atgagcgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac caatgtgcgt ttttggccta agaaatgccg ttacagcatt 240 agcacctttg tgccggggag ccagccgggc gagtttactt taggcatgat taaaagtcct 300 ccgggcggaa catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtgggtgtt ccagaaccgc gagttctttg agatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 105 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0466.13C10 <400> 105 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctg 120 gcactgcgtg aggataagga tccgaaaaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgcgt tttcgtcaca agaaatgcca atacgacatt 240 gcaacctttg tgccggggag ccagccgggc gagtttactt taggcctgat taaaagtgac 300 ccgggccaaa catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaagtggt tcagaaccgc gagttctttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 106 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0510.11H24 <400> 106 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcaga agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 107 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.01M12_X103Q <400> 107 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 actctgcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcgta agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggcgtgat taaaagtgat 300 ccgggccaga catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 108 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0512.01F10_X103Q <400> 108 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga catcagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 109 <211> 522 <212> ADN <213> Secuencia Artificial <220> <223> S0510.10A04_X103Q <400> 109 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg aggataagga tccgaagaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttcggtata agaaatgcca gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taagtgagcc gggccagaca 300 tcagagttgg tccgcgtcgt gagcaccaac tacaaccagc atgccatggt gttcttcaag 360 aaggtggtgc agaaccgcga gtttttttgg atcacactgt acgggcgcac gaaagaactg 420 acaagcgagc tgaaggaaaa ttttatccgc ttttccaaat ctctgggcct ccctgaaaac 480 cacatcgtct tccctgtccc aatcgaccag tgtatcgacg gc 522 <210> 110 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07G09 <400> 110 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctcggcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga caccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt ccagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 111 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07K20 <400> 111 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg aggataagaa tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttaccttcg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga cgccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgccaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 112 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07K21 <400> 112 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg agggtaggga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga caccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 113 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07G09_N65D <400> 113 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctcggcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga caccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt ccagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 114 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07K20_N65D <400> 114 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg aggataagaa tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttaccttcg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga cgccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgccaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 115 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07K21_N65D <400> 115 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctctgcgtg agggtaggga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga caccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt tcagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 116 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0530.07G09_H28Q_N65D <400> 116 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggcgaggc cggaaatctt 120 gctcggcgtg aggataagga tccgatgaaa atgatggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgaggtgagg tttaggcata agaaatgcaa gtacgatatt 240 gttacctttg tgccggggag ccagccgggc gagtttactt taggccttat taaaagtgat 300 ccgggccaga caccagagtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agaaggtggt ccagaaccgc gagtattttt ggatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 117 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0455.04F12 <400> 117 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcctggc cggaaatatc 120 ctgctgcgtg aggataagga tccgcacaaa atgctggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccacgtgacc tttaaatgga agaaatgcta ctacgcaatt 240 cgtacctttg tgccggggag ccagccgggc gagtttactt taggcatgat taaaagtgag 300 ccgggccaca catcaatgtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agtgggtgga ccagaaccgc gaggagtttc tgatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 118 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0455.04F23 <400> 118 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccacgc cggaaatcaa 120 tggctgcgtg aggataagga tccgcgtaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtggac tttgcaatca agaaatgcca ctaccgtatt 240 accacctttg tgccggggag ccagccgggc gagtttactt taggcaatat taaaagtcac 300 ccgggcggaa catcaggatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agttcgtgat ccagaaccgc gaggcatttt tcatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 119 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0455.04F09 <400> 119 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcatggc cggaaatttc 120 cacctgcgtg aggataagga tccgagcaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccacgtgcct ttttgggcaa agaaatgcgc atacaaaatt 240 atcacctttg tgccggggag ccagccgggc gagtttactt taggcgcaat taaaagtgga 300 ccgggcatga catcatggtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agggagtgtg gcagaaccgc gagacctttg ttatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 120 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0455.04M08 <400> 120 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcgttgc cggaaattac 120 tggctgcgtg aggataagga tccggcaaaa atgtacgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtgcgt ttttggcgta agaaatgccg ttacgacatt 240 tggacctttg tgccggggag ccagccgggc gagtttactt taggccctat taaaagtgag 300 ccgggccaaa catcacggtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agctggtgcg tcagaaccgc gaggcattta atatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 121 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0522.15K24 <400> 121 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcc tgggcatggc cggaaatttc 120 cacctgcgtg aggataagga tccgagcaag atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccacgtgcct ttttgggcaa agaaatgcgc atacaaaact 240 atcacctttg tgccggggag ccagccgggc gagtttactt taggcgcaat taaaagtgga 300 ccgggcatga catcatggtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttctcca agggagtgtg gcagaaccgc gagacctttg ttatcacact gtacgggcgc 420 gcgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 122 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0527.05N11 <400> 122 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggcatggc cggaaatttc 120 cacctgcgtg aggataagga tccgagcaaa atgcctgcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac ccacgtgcct ttttgggcaa agaaatgcgc atacaaaact 240 atcacctttg tgccggggag ccagccgggc gagtttactt taggcgcaat taaaagtgga 300 ccgggcatga catcatggtt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttctcca agggagtgtg gcagaaccgt gagacctttg ttatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 123 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0522.05A05 <400> 123 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccacgc cggaaatcaa 120 tggctgcgtg agggtaggga tccgcgtaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtggac tttgcaatca agaaatgcct ctaccgtatt 240 accacctttg tgccagggag ccagccgggc gagtttactt taggcaatat taaaagtcac 300 ccgggcggaa catcaggatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agttcgtgat ccagaaccgc gaggcatttt tcatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 124 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0522.05M06 <400> 124 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccacgc cggaaatcaa 120 tggctgcgtg gggattacga tccgcgtaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat ataacgtcac cgacgtggac tttgcaatcg agaaatgcca ctaccgtatt 240 accacctttg tgccggggag ccagccgggc gagtttactt ttggcaatat aaaaagtcac 300 ccgggcggaa catcaggatt ggcccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agttcgtgat ccagaaccgc gaggcatttt tcatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 125 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0522.05A05_H28Q_N65D <400> 125 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccaagggaaa tggtatgtcg tgggccacgc cggaaatcaa 120 tggctgcgtg agggtaggga tccgcgtaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgacgtggac tttgcaatca agaaatgcct ctaccgtatt 240 accacctttg tgccagggag ccagccgggc gagtttactt taggcaatat taaaagtcac 300 ccgggcggaa catcaggatt ggtccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agttcgtgat ccagaaccgc gaggcatttt tcatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 126 <211> 534 <212> ADN <213> Secuencia Artificial <220> <223> S0522.05M06_N65D <400> 126 caggactcca cctcagacct gatcccagcc ccacctctga gcaaggtccc tctgcagcag 60 aacttccagg acaaccaatt ccatgggaaa tggtatgtcg tgggccacgc cggaaatcaa 120 tggctgcgtg gggattacga tccgcgtaaa atgtgggcga ccatttacga gttgaaagaa 180 gataaatcat atgacgtcac cgacgtggac tttgcaatcg agaaatgcca ctaccgtatt 240 accacctttg tgccggggag ccagccgggc gagtttactt ttggcaatat aaaaagtcac 300 ccgggcggaa catcaggatt ggcccgcgtc gtgagcacca actacaacca gcatgccatg 360 gtgttcttca agttcgtgat ccagaaccgc gaggcatttt tcatcacact gtacgggcgc 420 acgaaagaac tgacaagcga gctgaaggaa aattttatcc gcttttccaa atctctgggc 480 ctccctgaaa accacatcgt cttccctgtc ccaatcgacc agtgtatcga cggc 534 <210> 127 <211> 10 <212> PRT <213> Secuencia Artificial <220> <223> Secuencia C-terminal de muteínas <400> 127 Ser Ala Trp Ser His Pro Gln Phe Glu Lys 1 5 10 <210> 128 <211> 8 <212> PRT <213> Secuencia Artificial <220> <223> Conector SA y la Strep Tag II <400> 128 Trp Ser His Pro Gln Phe Glu Lys 1 5 <210> 129 <211> 200 <212> PRT <213> Secuencia Artificial <220> <223> Secuencia de aminoácidos de proteína expresada <400> 129 Met Lys His His His His His His Asp Tyr Asp Ile Pro Thr Thr Glu 1 5 10 15 Asn Leu Tyr Phe Gln Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 20 25 30 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 35 40 45 Phe His Gly Lys Trp Tyr Val Val Gly Val Ala Gly Asn Thr Ile Leu 50 55 60 Arg Glu Asp Lys Asp Pro Gly Lys Met Asn Ala Thr Ile Tyr Glu Leu 65 70 75 80 Lys Glu Asp Lys Ser Tyr Asn Val Thr Asp Val Arg Phe Ile Arg Lys 85 90 95 Lys Cys His Tyr Tyr Ile Asp Thr Phe Val Pro Gly Ser Gln Pro Gly 100 105 110 Glu Phe Thr Leu Gly Asn Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln 115 120 125 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 130 135 140 Phe Lys Ile Val Arg Gln Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr 145 150 155 160 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 165 170 175 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 180 185 190 Pro Ile Asp Gln Cys Ile Asp Gly 195 200 <210> 130 <211> 4 <212> PRT <213> Secuencia Artificial <220> <223> posiciones 95-98 <400> 130 Gly Asn Ile Lys 1 <210> 131 <211> 6 <212> PRT <213> Secuencia Artificial <220> <223> ejemplos de péptidos de unión a albúmina <220> <221> CARACTERÍSTICA_MISC <222> (2)..(2) <223> Xaa can be any of Asp, Asn, Ser, Thr, or Trp <220> <221> CARACTERÍSTICA_MISC <222> (3)..(3) <223> Xaa can be any of Asn, Gln, His, Ile, Leu, or Lys <220> <221> CARACTERÍSTICA_MISC <222> (4)..(4) <223> Xaa can be any of Ala, Asp, Phe, Trp, or Tyr <220> <221> CARACTERÍSTICA_MISC <222> (5)..(5) <223> Xaa can be any of Asp, Gly, Leu, Phe, Ser, or Thr <400> 131 Cys Xaa Xaa Xaa Xaa Cys 1 5 <210> 132 <211> 7 <212> PRT <213> Secuencia Artificial <220> <223> Conector <400> 132 Asp Tyr Asp Ile Pro Thr Thr 1 5 <210> 133 <211> 7 <212> PRT <213> Secuencia Artificial <220> <223> sitio de escisión de proteasa <400> 133 Glu Asn Leu Tyr Phe Gln Gly 1 5 <210> 134 <211> 742 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_H28Q_T54A_N65D_(G4S)2S0544.04C04_H28Q_N65D_(G4S)2 S0530.07G09_H28Q_N65D_(G4S)2 S0522.05A05_H28Q_N65D <400> 134 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Asn Thr Ala Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe Gln Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu 595 600 605 Gly Arg Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys 625 630 635 640 Leu Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly 740 <210> 135 <211> 742 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65D_(G4S)2S0530.04N16_N65D_(G4S)2 S0530.07G09_N65D_(G4S)2 S0522.05M06_N65D <400> 135 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly 595 600 605 Asp Tyr Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Phe Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly 740 <210> 136 <211> 980 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_H28Q_T54A_N65D(G4S)2S0544.04C04_H28Q_N65D(G4S)2 S0530.07G09_H28Q_N65D_(G4S)2 S0522.05A05_H28Q_N65D_(G4S)2 huIgG4Fc <400> 136 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Asn Thr Ala Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe Gln Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu 595 600 605 Gly Arg Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys 625 630 635 640 Leu Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 740 745 750 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 755 760 765 Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 770 775 780 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 785 790 795 800 Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 805 810 815 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 820 825 830 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 835 840 845 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 850 855 860 Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 865 870 875 880 Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 885 890 895 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 900 905 910 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 915 920 925 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 930 935 940 Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 945 950 955 960 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 965 970 975 Leu Ser Leu Gly 980 <210> 137 <211> 980 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65D_(G4S)2 S0530.04N16_N65D_(G4S)2_ S0530.07G09_N65D_(G4S)2_ S0522.05M06_N65D_(G4S)2_hIgG4Fc <400> 137 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly 595 600 605 Asp Tyr Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Phe Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 740 745 750 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 755 760 765 Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 770 775 780 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 785 790 795 800 Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 805 810 815 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 820 825 830 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 835 840 845 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 850 855 860 Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 865 870 875 880 Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 885 890 895 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 900 905 910 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 915 920 925 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 930 935 940 Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 945 950 955 960 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 965 970 975 Leu Ser Leu Gly 980 <210> 138 <211> 980 <212> PRT <213> Secuencia Artificial <220> <223> hIgG4Fc_(G4S)2_S0474.01H22_T54A_N65D_(G4S)2S0530.04N16_N65D_(G4S) 2_S0530.07G09_N65D_(G4S)2_ S0522.05M06_N65D <400> 138 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 1 5 10 15 Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 20 25 30 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 35 40 45 Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 50 55 60 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 65 70 75 80 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 85 90 95 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 100 105 110 Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 115 120 125 Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 130 135 140 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 145 150 155 160 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 165 170 175 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 180 185 190 Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 195 200 205 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 210 215 220 Leu Ser Leu Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp 225 230 235 240 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 245 250 255 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 260 265 270 Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro Gly Lys 275 280 285 Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val 290 295 300 Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile Asp Thr 305 310 315 320 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn Ile Lys 325 330 335 Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn 340 345 350 Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln Asn Arg 355 360 365 Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 370 375 380 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 385 390 395 400 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 405 410 415 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp 420 425 430 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 435 440 445 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 450 455 460 Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met Ala Ala 465 470 475 480 Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp 485 490 495 Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val Pro Gly 500 505 510 Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly 515 520 525 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 530 535 540 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 545 550 555 560 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 565 570 575 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 580 585 590 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly 595 600 605 Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 610 615 620 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 625 630 635 640 Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Arg 645 650 655 Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu 660 665 670 Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg His Lys 675 680 685 Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly 690 695 700 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu 705 710 715 720 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 725 730 735 Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr 740 745 750 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 755 760 765 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 770 775 780 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly 785 790 795 800 Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 805 810 815 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 820 825 830 Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly Asp Tyr 835 840 845 Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 850 855 860 Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys His Tyr 865 870 875 880 Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe 885 890 895 Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala Arg Val 900 905 910 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val 915 920 925 Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys 930 935 940 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 945 950 955 960 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 965 970 975 Cys Ile Asp Gly 980 <210> 139 <211> 980 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22_T54A_N65D_(G4S)2S0530.04N16_N65D_(G4S)2_hIgG4Fc_S0530 .07G09_N65D_(G4S)2 S0522.05M06_N65D <400> 139 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro Pro Cys 370 375 380 Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu 385 390 395 400 Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 405 410 415 Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 420 425 430 Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 435 440 445 Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 450 455 460 Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 465 470 475 480 Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 485 490 495 Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 500 505 510 Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 515 520 525 Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 530 535 540 Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 545 550 555 560 Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 565 570 575 Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 580 585 590 His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Gly Gly Gly 595 600 605 Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 610 615 620 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 625 630 635 640 Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Arg 645 650 655 Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu 660 665 670 Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg His Lys 675 680 685 Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly 690 695 700 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu 705 710 715 720 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 725 730 735 Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr 740 745 750 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 755 760 765 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 770 775 780 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly 785 790 795 800 Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 805 810 815 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 820 825 830 Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly Asp Tyr 835 840 845 Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 850 855 860 Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys His Tyr 865 870 875 880 Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Phe 885 890 895 Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala Arg Val 900 905 910 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val 915 920 925 Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys 930 935 940 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 945 950 955 960 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 965 970 975 Cys Ile Asp Gly 980 <210> 140 <211> 228 <212> PRT <213> Secuencia Artificial <220> <223> Fc de IgG4 <400> 140 Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 1 5 10 15 Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 20 25 30 Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 35 40 45 Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 50 55 60 Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 65 70 75 80 Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 85 90 95 Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 100 105 110 Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 115 120 125 Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 130 135 140 Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 145 150 155 160 Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 165 170 175 Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 180 185 190 Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 195 200 205 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 210 215 220 Leu Ser Leu Gly 225 <210> 141 <211> 10 <212> PRT <213> Secuencia Artificial <220> <223> Conector <400> 141 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 <210> 142 <211> 6 <212> PRT <213> Secuencia Artificial <220> <223> Conector <400> 142 Gly Ser Gly Gly Ser Gly 1 5 <210> 143 <211> 980 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(H28Q;T54A;N65D) (G4S)2 S0544.04C04(H28Q;N65D) (G4S)2 (FC;HuIgG4) (G4S)2 S0530.07G09(H28Q;N65D) (G4S)2 S0522.05A05(H28Q;N65D) <400> 143 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Asn Thr Ala Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Val Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Glu Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Thr Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly Pro Pro Cys 370 375 380 Pro Pro Cys Pro Ala Pro Glu Phe Glu Gly Gly Pro Ser Val Phe Leu 385 390 395 400 Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 405 410 415 Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 420 425 430 Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 435 440 445 Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 450 455 460 Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 465 470 475 480 Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 485 490 495 Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 500 505 510 Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 515 520 525 Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 530 535 540 Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 545 550 555 560 Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 565 570 575 Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 580 585 590 His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Gly Gly Gly Gly 595 600 605 Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 610 615 620 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 625 630 635 640 Phe Gln Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Arg 645 650 655 Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu 660 665 670 Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg His Lys 675 680 685 Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly 690 695 700 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu 705 710 715 720 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 725 730 735 Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr 740 745 750 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 755 760 765 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 770 775 780 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly 785 790 795 800 Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 805 810 815 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe Gln Gly Lys 820 825 830 Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Gly Arg 835 840 845 Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 850 855 860 Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys Leu Tyr 865 870 875 880 Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 885 890 895 Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val 900 905 910 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val 915 920 925 Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys 930 935 940 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 945 950 955 960 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 965 970 975 Cys Ile Asp Gly 980 <210> 144 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(T54A;N65D) GQPKAAPS S0530.04N16(N65D) GQPKAAPS S0530.07G09(N65Q) GQPKAAPS S0522.05A05(N65D) Cterm StrepII 6His <400> 144 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met Ala Ala 225 230 235 240 Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp 245 250 255 Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val Pro Gly 260 265 270 Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys 355 360 365 Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Arg Arg Glu 405 410 415 Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys 435 440 445 Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly His Ala Gly Asn Gln Trp Leu Arg Glu Gly Arg Asp Pro Arg Lys 595 600 605 Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val 610 615 620 Thr Asp Val Asp Phe Ala Ile Lys Lys Cys Leu Tyr Arg Ile Thr Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn Ile Lys 645 650 655 Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln Asn Arg 675 680 685 Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 145 <211> 746 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(T54A;N65D) GSGGSG S0530.04N16(N65D) GSGGSG S0530.07G09(N65Q) GSGGSG S0522.05A05(N65D) Cterm StrepII 6His <400> 145 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Ser Gly Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile 180 185 190 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 195 200 205 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly 210 215 220 Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met Ala Ala Val Tyr 225 230 235 240 Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu 245 250 255 Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val Pro Gly Asn Gln 260 265 270 Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr 275 280 285 Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 290 295 300 Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr 305 310 315 320 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 325 330 335 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 340 345 350 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Ser Gly Gly Ser Gly 355 360 365 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 370 375 380 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 385 390 395 400 Val Val Gly Glu Ala Gly Asn Leu Ala Arg Arg Glu Asp Lys Asp Pro 405 410 415 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 420 425 430 Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Lys Tyr Asp Ile 435 440 445 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 450 455 460 Ile Lys Ser Asp Pro Gly Gln Thr Pro Glu Leu Val Arg Val Val Ser 465 470 475 480 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 485 490 495 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 500 505 510 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 515 520 525 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 530 535 540 Asp Gly Gly Ser Gly Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile 545 550 555 560 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 565 570 575 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln 580 585 590 Trp Leu Arg Glu Gly Arg Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr 595 600 605 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala 610 615 620 Ile Lys Lys Cys Leu Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln 625 630 635 640 Pro Gly Glu Phe Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr 645 650 655 Ser Gly Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 660 665 670 Val Phe Phe Lys Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr 675 680 685 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 690 695 700 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 705 710 715 720 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro 725 730 735 Gln Phe Glu Lys His His His His His His 740 745 <210> 146 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(T54A;N65D) (G4S)2 S0530.04N16(N65D) (G4S)2 S0530.07G09(N65Q) (G4S)2 S0522.05A05(N65D) Cterm StrepII 6His <400> 146 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu 595 600 605 Gly Arg Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys 625 630 635 640 Leu Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 147 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) (G4S)2 S0530.04N16(N65Q) (G4S)2 S0530.07K20(N65Q) (G4S)2 S0522.15K24(N65Q) Cterm StrepII His <400> 147 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Gln Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asn Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Pro Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Leu Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Thr Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Ala Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 148 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) (G4S)2 S0530.04N16(N65Q) (G4S)2 S0530.07K20(N65Q) (G4S)2 S0522.05M06 (N65Q) Cterm StrepII His <400> 148 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Gln Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asn Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Pro Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly 595 600 605 Asp Tyr Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Gln Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Phe Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 149 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) (G4S)2 S0530.04N16(N65Q) (G4S)2 S0530.07G09(N65Q) (G4S)2 S0522.15K24(N65Q) Cterm StrepII His <400> 149 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Gln Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Leu Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Thr Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Ala Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 150 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0530.04N16(N65D) (G4S)2 S0530.07K20(N65D) (G4S)2 S0522.15K24(N65D) Cterm StrepII His <400> 150 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asn Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Pro Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Leu Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Thr Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Ala Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 151 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0530.04N16(N65D) (G4S)2 S0530.07K20(N65D) (G4S)2 S0522.05M06 (N65D) Cterm StrepII His <400> 151 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asn Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Pro Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly 595 600 605 Asp Tyr Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Phe Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 152 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0530.04N16(N65D) (G4S)2 S0530.07G09(N65D) (G4S)2 S0522.15K24(N65D) Cterm StrepII His <400> 152 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Leu Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Thr Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Ser Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Ala Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 153 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0530.04N16(N65D) (G4S)2 S0530.07G09(N65D) (G4S)2 S0522.05M06 (N65D) Cterm StrepII His <400> 153 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Glu Asp Pro Leu Lys Met Met 225 230 235 240 Ala Ala Val Tyr Glu Leu Arg Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Glu Glu Cys Arg Tyr Met Thr Glu Thr Phe Val 260 265 270 Pro Gly Asn Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Arg Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Pro Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Gly 595 600 605 Asp Tyr Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Glu Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Phe Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Ala 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 154 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GQPKAAPS S0466.09E05(N65Q) GQPKAAPS S0466.13C10(N65Q) GQPKAAPS S0455.04F09(N65Q) StrepII, His6 <400> 154 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr 225 230 235 240 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp 245 250 255 Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly 260 265 270 Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys 355 360 365 Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 405 410 415 Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys 435 440 445 Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys 595 600 605 Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val 610 615 620 Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys 645 650 655 Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg 675 680 685 Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 155 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GQPKAAPS S0466.09E05(N65Q) GQPKAAPS S0466.13C10(N65Q) GQPKAAPS S0455.04F09(N65Q) StrepII, His6 <400> 155 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr 225 230 235 240 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp 245 250 255 Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly 260 265 270 Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys 355 360 365 Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 405 410 415 Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys 435 440 445 Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys 595 600 605 Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val 610 615 620 Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys 645 650 655 Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg 675 680 685 Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 156 <211> 746 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) ASTKGP S0466.09E05(N65Q) ASTKGP S0466.13C10(N65Q) ASTKGP S0455.04F09(N65Q) StrepII, His6 <400> 156 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Ala Ser Thr Lys Gly Pro Gln Asp Ser Thr Ser Asp Leu Ile 180 185 190 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 195 200 205 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly 210 215 220 Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile Tyr 225 230 235 240 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe Glu 245 250 255 Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln 260 265 270 Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp Thr 275 280 285 Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 290 295 300 Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr 305 310 315 320 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 325 330 335 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 340 345 350 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ala Ser Thr Lys Gly Pro 355 360 365 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 370 375 380 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 385 390 395 400 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 405 410 415 Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 420 425 430 Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr Asp Ile 435 440 445 Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 450 455 460 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 465 470 475 480 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 485 490 495 Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 500 505 510 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 515 520 525 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 530 535 540 Asp Gly Ala Ser Thr Lys Gly Pro Gln Asp Ser Thr Ser Asp Leu Ile 545 550 555 560 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 565 570 575 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe 580 585 590 His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr 595 600 605 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp 610 615 620 Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln 625 630 635 640 Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr 645 650 655 Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 660 665 670 Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr 675 680 685 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 690 695 700 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 705 710 715 720 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro 725 730 735 Gln Phe Glu Lys His His His His His His 740 745 <210> 157 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) ASTKGPS S0466.09E05(N65Q) ASTKGPS S0466.13C10(N65Q) ASTKGPS S0455.04F09(N65Q) StrepII, His6 <400> 157 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Ala Ser Thr Lys Gly Pro Ser Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ala Ser Thr Lys Gly 355 360 365 Pro Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr 435 440 445 Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Ala Ser Thr Lys Gly Pro Ser Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 158 <211> 746 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) QPKAAP S0466.09E05(N65Q) QPKAAP S0466.13C10(N65Q) QPKAAP S0455.04F09(N65Q) StrepII, His6 <400> 158 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gln Pro Lys Ala Ala Pro Gln Asp Ser Thr Ser Asp Leu Ile 180 185 190 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 195 200 205 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly 210 215 220 Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile Tyr 225 230 235 240 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe Glu 245 250 255 Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln 260 265 270 Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp Thr 275 280 285 Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 290 295 300 Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr 305 310 315 320 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 325 330 335 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 340 345 350 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gln Pro Lys Ala Ala Pro 355 360 365 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 370 375 380 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 385 390 395 400 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 405 410 415 Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 420 425 430 Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr Asp Ile 435 440 445 Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 450 455 460 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 465 470 475 480 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 485 490 495 Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 500 505 510 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 515 520 525 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 530 535 540 Asp Gly Gln Pro Lys Ala Ala Pro Gln Asp Ser Thr Ser Asp Leu Ile 545 550 555 560 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 565 570 575 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe 580 585 590 His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr 595 600 605 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp 610 615 620 Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln 625 630 635 640 Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr 645 650 655 Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 660 665 670 Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr 675 680 685 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 690 695 700 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 705 710 715 720 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro 725 730 735 Gln Phe Glu Lys His His His His His His 740 745 <210> 159 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GQPKAAP S0466.09E05(N65Q) GQPKAAP S0466.13C10(N65Q) GQPKAAP S0455.04F09(N65Q) StrepII, His6 <400> 159 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Pro Lys Ala Ala Pro Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys Ala 355 360 365 Ala Pro Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr 435 440 445 Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Gly Gln Pro Lys Ala Ala Pro Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 160 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GQPKAAPS S0466.09E05(N65Q) GQPKAAPS S0466.13C10(N65Q) GQPKAAPS S0455.04F09(N65Q) StrepII, His6 <400> 160 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr 225 230 235 240 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp 245 250 255 Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly 260 265 270 Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys 355 360 365 Ala Ala Pro Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 405 410 415 Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys 435 440 445 Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gln Pro Lys Ala Ala Pro Ser Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys 595 600 605 Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val 610 615 620 Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys 645 650 655 Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg 675 680 685 Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 161 <211> 743 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GGSGG S0466.09E05(N65Q) GGSGG S0466.13C10(N65Q) GGSGG S0455.04F09(N65Q) StrepII, His6 <400> 161 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro 180 185 190 Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn 195 200 205 Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp 210 215 220 Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile Tyr Glu 225 230 235 240 Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe Glu Leu 245 250 255 Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro 260 265 270 Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp Thr Ser 275 280 285 Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val 290 295 300 Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu 305 310 315 320 Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile 325 330 335 Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro 340 345 350 Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Gln Asp 355 360 365 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 370 375 380 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 385 390 395 400 Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys 405 410 415 Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val 420 425 430 Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr Asp Ile Ala Thr 435 440 445 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys 450 455 460 Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser Thr Asn 465 470 475 480 Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln Asn Arg 485 490 495 Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 500 505 510 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 515 520 525 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 530 535 540 Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 545 550 555 560 Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 565 570 575 His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg 580 585 590 Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys 595 600 605 Glu Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys 610 615 620 Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 625 630 635 640 Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu 645 650 655 Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 660 665 670 Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly 675 680 685 Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 690 695 700 Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 705 710 715 720 Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu 725 730 735 Lys His His His His His His 740 <210> 162 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A)SGGSG S0466.09E05(N65Q) SGGSG S0466.13C10(N65Q) SGGSG S0455.04F09(N65Q) StrepII, His6 <400> 162 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Ser Gly 355 360 365 Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr 435 440 445 Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 163 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GGGSGGG S0466.09E05(N65Q) GGGSGGG S0466.13C10(N65Q) GGGSGGG S0455.04F09(N65Q) StrepII, His6 <400> 163 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Ser Gly 355 360 365 Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys Gln Tyr 435 440 445 Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 164 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A)GGSGGSGG S0466.09E05(N65Q) GGSGGSGG S0466.13C10(N65Q)GGSGGSGG S0455.04F09(N65Q) StrepII, His6 <400> 164 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala Thr 225 230 235 240 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val Asp 245 250 255 Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly 260 265 270 Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly 355 360 365 Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 405 410 415 Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys Lys Cys 435 440 445 Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys 595 600 605 Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val 610 615 620 Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys 645 650 655 Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg 675 680 685 Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 165 <211> 755 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) GGGSGGSGGG S0466.09E05(N65Q) GGGSGGSGGG S0466.13C10(N65Q) GGGSGGSGGG S0455.04F09(N65Q) StrepII, His6 <400> 165 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser 180 185 190 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 195 200 205 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala 210 215 220 Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala 225 230 235 240 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val 245 250 255 Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro 260 265 270 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro 275 280 285 Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 290 295 300 His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe 305 310 315 320 His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 325 330 335 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 340 345 350 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser 355 360 365 Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 370 375 380 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 385 390 395 400 Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu 405 410 415 Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu 420 425 430 Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys 435 440 445 Lys Cys Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly 450 455 460 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu 465 470 475 480 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 485 490 495 Phe Lys Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr 500 505 510 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 515 520 525 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 530 535 540 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly 545 550 555 560 Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys 565 570 575 Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp 580 585 590 Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp 595 600 605 Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser 610 615 620 Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys 625 630 635 640 Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly 645 650 655 Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val 660 665 670 Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp 675 680 685 Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu 690 695 700 Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu 705 710 715 720 Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys 725 730 735 Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His 740 745 750 His His His 755 <210> 166 <211> 755 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A)GGGSGGSGGG S0466.09E05(N65Q) GGGSGGSGGG S0466.13C10(N65Q)GGGSGGSGGG S0455.04F09(N65Q) StrepII, His6 <400> 166 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser 180 185 190 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 195 200 205 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala 210 215 220 Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met Ala 225 230 235 240 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val Val 245 250 255 Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro 260 265 270 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe Pro 275 280 285 Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 290 295 300 His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe 305 310 315 320 His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 325 330 335 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 340 345 350 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser 355 360 365 Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 370 375 380 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 385 390 395 400 Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu 405 410 415 Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr Glu Leu 420 425 430 Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg His Lys 435 440 445 Lys Cys Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln Pro Gly 450 455 460 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu 465 470 475 480 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 485 490 495 Phe Lys Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr 500 505 510 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 515 520 525 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 530 535 540 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly 545 550 555 560 Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys 565 570 575 Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp 580 585 590 Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp 595 600 605 Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser 610 615 620 Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys 625 630 635 640 Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly 645 650 655 Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val 660 665 670 Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp 675 680 685 Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu 690 695 700 Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu 705 710 715 720 Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys 725 730 735 Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His 740 745 750 His His His 755 <210> 167 <211> 728 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) S0510.05H10(N65D) S0510.11H24(N65D) S0455.04F09(N65D) StrepII His etiqueta <400> 167 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 180 185 190 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 195 200 205 Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys 210 215 220 Asp Pro Leu Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 225 230 235 240 Ser Tyr Asp Val Thr Val Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr 245 250 255 Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 260 265 270 Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg Val 275 280 285 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val 290 295 300 Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr Lys 305 310 315 320 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 325 330 335 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 340 345 350 Cys Ile Asp Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 355 360 365 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 370 375 380 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 385 390 395 400 Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 405 410 415 Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys 420 425 430 Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 435 440 445 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 450 455 460 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 465 470 475 480 Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg 485 490 495 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 500 505 510 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 515 520 525 Asp Gln Cys Ile Asp Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 530 535 540 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 545 550 555 560 Phe His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu 565 570 575 Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu 580 585 590 Lys Glu Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys 595 600 605 Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly 610 615 620 Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp 625 630 635 640 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 645 650 655 Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr 660 665 670 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 675 680 685 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 690 695 700 Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe 705 710 715 720 Glu Lys His His His His His His 725 <210> 168 <211> 731 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G) S0510.05H10(N65D) (G) S0510.11H24(N65D) (G) S0455.04F09(N65D) StrepII His etiqueta <400> 168 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu 180 185 190 Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly 195 200 205 Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg Glu Asp 210 215 220 Lys Asp Pro Leu Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp 225 230 235 240 Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu Leu Lys Lys Cys Arg 245 250 255 Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr 260 265 270 Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu Val Arg 275 280 285 Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly 290 295 300 Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly Arg Thr 305 310 315 320 Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys 325 330 335 Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp 340 345 350 Gln Cys Ile Asp Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala 355 360 365 Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln 370 375 380 Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu 385 390 395 400 Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu 405 410 415 Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg Gln Lys 420 425 430 Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly 435 440 445 Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu 450 455 460 Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe 465 470 475 480 Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr 485 490 495 Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg 500 505 510 Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val 515 520 525 Pro Ile Asp Gln Cys Ile Asp Gly Gly Gln Asp Ser Thr Ser Asp Leu 530 535 540 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 545 550 555 560 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn 565 570 575 Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile 580 585 590 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe 595 600 605 Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser 610 615 620 Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met 625 630 635 640 Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 645 650 655 Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile 660 665 670 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 675 680 685 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 690 695 700 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His 705 710 715 720 Pro Gln Phe Glu Lys His His His His His His 725 730 <210> 169 <211> 737 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G)3 S0510.05H10(N65D) (G)3 S0510.11H24(N65D) (G)3 S0455.04F09(N65D) StrepII His etiqueta <400> 169 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 180 185 190 Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 195 200 205 His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp Leu Arg 210 215 220 Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile Tyr Glu Leu Lys 225 230 235 240 Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu Leu Lys Lys 245 250 255 Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 260 265 270 Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr Ser Gln Leu 275 280 285 Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 290 295 300 Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu Tyr Gly 305 310 315 320 Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 325 330 335 Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 340 345 350 Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gln Asp Ser Thr Ser Asp 355 360 365 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 370 375 380 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly 385 390 395 400 Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr 405 410 415 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg 420 425 430 Phe Arg Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly 435 440 445 Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly 450 455 460 Gln Thr Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 465 470 475 480 Ala Met Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp 485 490 495 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 500 505 510 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 515 520 525 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gln 530 535 540 Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro 545 550 555 560 Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val 565 570 575 Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser 580 585 590 Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp 595 600 605 Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile 610 615 620 Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile 625 630 635 640 Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr 645 650 655 Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn 660 665 670 Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr 675 680 685 Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu 690 695 700 Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp 705 710 715 720 Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His 725 730 735 His <210> 170 <211> 743 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (GSGSG) S0510.05H10(N65D) (GSGSG) S0510.11H24(N65D) (GSGSG) S0455.04F09(N65D) StrepII His etiqueta <400> 170 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Ser Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro 180 185 190 Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn 195 200 205 Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp 210 215 220 Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile Tyr Glu 225 230 235 240 Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu Leu 245 250 255 Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro 260 265 270 Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr Ser 275 280 285 Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val 290 295 300 Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu 305 310 315 320 Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile 325 330 335 Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro 340 345 350 Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Ser Gly Ser Gly Gln Asp 355 360 365 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 370 375 380 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 385 390 395 400 Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro Met Lys 405 410 415 Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val 420 425 430 Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr 435 440 445 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys 450 455 460 Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser Thr Asn 465 470 475 480 Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln Asn Arg 485 490 495 Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 500 505 510 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 515 520 525 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 530 535 540 Gly Ser Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 545 550 555 560 Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 565 570 575 His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg 580 585 590 Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys 595 600 605 Glu Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys 610 615 620 Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 625 630 635 640 Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu 645 650 655 Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 660 665 670 Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly 675 680 685 Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 690 695 700 Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 705 710 715 720 Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu 725 730 735 Lys His His His His His His 740 <210> 171 <211> 743 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G2SG2) S0510.05H10(N65D) (G2SG2) S0510.11H24(N65D) (G2SG2) S0455.04F09(N65D) StrepII His etiqueta <400> 171 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro 180 185 190 Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn 195 200 205 Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly Trp 210 215 220 Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile Tyr Glu 225 230 235 240 Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu Leu 245 250 255 Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln Pro 260 265 270 Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr Ser 275 280 285 Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val 290 295 300 Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr Leu 305 310 315 320 Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile 325 330 335 Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro 340 345 350 Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Gln Asp 355 360 365 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 370 375 380 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 385 390 395 400 Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro Met Lys 405 410 415 Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val 420 425 430 Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr 435 440 445 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys 450 455 460 Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser Thr Asn 465 470 475 480 Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln Asn Arg 485 490 495 Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 500 505 510 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 515 520 525 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 530 535 540 Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro 545 550 555 560 Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe 565 570 575 His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg 580 585 590 Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys 595 600 605 Glu Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys 610 615 620 Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu 625 630 635 640 Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu 645 650 655 Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe 660 665 670 Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly 675 680 685 Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe 690 695 700 Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro 705 710 715 720 Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu 725 730 735 Lys His His His His His His 740 <210> 172 <211> 746 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (GSG2SG) S0510.05H10(N65D) (GSG2SG) S0510.11H24(N65D) (GSG2SG) S0455.04F09(N65D) StrepII His etiqueta <400> 172 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Ser Gly Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile 180 185 190 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 195 200 205 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn Gly 210 215 220 Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile Tyr 225 230 235 240 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe Glu 245 250 255 Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser Gln 260 265 270 Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp Thr 275 280 285 Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 290 295 300 Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile Thr 305 310 315 320 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 325 330 335 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 340 345 350 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Ser Gly Gly Ser Gly 355 360 365 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 370 375 380 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 385 390 395 400 Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys Asp Pro 405 410 415 Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 420 425 430 Asp Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr Asp Ile 435 440 445 Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Leu 450 455 460 Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val Val Ser 465 470 475 480 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Val Gln 485 490 495 Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 500 505 510 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 515 520 525 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 530 535 540 Asp Gly Gly Ser Gly Gly Ser Gly Gln Asp Ser Thr Ser Asp Leu Ile 545 550 555 560 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 565 570 575 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe 580 585 590 His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr 595 600 605 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp 610 615 620 Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln 625 630 635 640 Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr 645 650 655 Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 660 665 670 Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr 675 680 685 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 690 695 700 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 705 710 715 720 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro 725 730 735 Gln Phe Glu Lys His His His His His His 740 745 <210> 173 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G2SGSG2) S0510.05H10(N65D) (G2SGSG2) S0510.11H24(N65D) (G2SGSG2) S0455.04F09(N65D) StrepII His etiqueta <400> 173 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Ser 355 360 365 Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Asp Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr 435 440 445 Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Gly Gly Ser Gly Ser Gly Gly Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 174 <211> 749 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G3SG3) S0510.05H10(N65D) (G3SG3) S0510.11H24(N65D) (G3SG3) S0455.04F09(N65D) StrepII His etiqueta <400> 174 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu 180 185 190 Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln 195 200 205 Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly Asn 210 215 220 Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr Ile 225 230 235 240 Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp Phe 245 250 255 Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly Ser 260 265 270 Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly Trp 275 280 285 Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala 290 295 300 Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His Ile 305 310 315 320 Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn 325 330 335 Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val 340 345 350 Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Ser Gly 355 360 365 Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser 370 375 380 Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys 385 390 395 400 Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu Asp Lys 405 410 415 Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys 420 425 430 Ser Tyr Asp Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys Lys Tyr 435 440 445 Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu 450 455 460 Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val Arg Val 465 470 475 480 Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val 485 490 495 Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys 500 505 510 Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser 515 520 525 Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln 530 535 540 Cys Ile Asp Gly Gly Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser 545 550 555 560 Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn 565 570 575 Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Met Ala 580 585 590 Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys Met Pro Ala 595 600 605 Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr His Val 610 615 620 Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr Phe Val Pro 625 630 635 640 Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys Ser Gly Pro 645 650 655 Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln 660 665 670 His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg Glu Thr Phe 675 680 685 Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys 690 695 700 Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His 705 710 715 720 Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Ser Ala Trp 725 730 735 Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 <210> 175 <211> 752 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G2SG2SG2) S0510.05H10(N65D) (G2SG2SG2) S0510.11H24(N65D) (G2SG2SG2) S0455.04F09(N65D) StrepII His etiqueta <400> 175 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp 180 185 190 Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe 195 200 205 Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu Ala Gly 210 215 220 Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met Ala Thr 225 230 235 240 Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val Val Asp 245 250 255 Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val Pro Gly 260 265 270 Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser Pro Gly 275 280 285 Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His 290 295 300 Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp Phe His 305 310 315 320 Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu 325 330 335 Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile 340 345 350 Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly 355 360 365 Gly Ser Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 370 375 380 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 385 390 395 400 Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu Ala Leu Arg Glu 405 410 415 Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr Glu Leu Lys Glu 420 425 430 Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg Gln Lys Lys Cys 435 440 445 Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 450 455 460 Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Ser Gly Gly Ser Gly Gly Gln Asp 545 550 555 560 Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu 565 570 575 Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val 580 585 590 Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro Ser Lys 595 600 605 Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val 610 615 620 Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile Ile Thr 625 630 635 640 Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala Ile Lys 645 650 655 Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser Thr Asn 660 665 670 Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln Asn Arg 675 680 685 Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser 690 695 700 Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro 705 710 715 720 Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly 725 730 735 Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His His His 740 745 750 <210> 176 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G3SG2SG3) S0510.05H10(N65D) (G3SG2SG3) S0510.11H24(N65D) (G3SG2SG3) S0455.04F09(N65D) StrepII His etiqueta <400> 176 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Ser Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Ser Gly Gly Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Ser Gly Gly 545 550 555 560 Ser Gly Gly Gly Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 177 <211> 754 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0512.17L07(N39S_N65D_T136A) (G4S)2 S0510.10A04_stop103Q(N65D) (G4S)2-S0455.04F23(N65D) StrepII His etiqueta <400> 177 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Ser Thr Thr Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Lys Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Tyr Lys Lys Cys Gln Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Ser Glu Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 545 550 555 560 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 565 570 575 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 580 585 590 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Asp Lys Asp Pro 595 600 605 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 610 615 620 Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys His Tyr Arg Ile 625 630 635 640 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 645 650 655 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser 660 665 670 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 675 680 685 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 690 695 700 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 705 710 715 720 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 725 730 735 Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His 740 745 750 His His <210> 178 <211> 754 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0512.17L07(N39S_N65D_T136A) (G4S)2-S0510.10A04_stop103Q(N65D) (G4S)2-S0455.04F09(N65D)StrepII His etiqueta <400> 178 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Ser Thr Thr Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Lys Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Tyr Lys Lys Cys Gln Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Ser Glu Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 545 550 555 560 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 565 570 575 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 580 585 590 Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro 595 600 605 Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 610 615 620 Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile 625 630 635 640 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 645 650 655 Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser 660 665 670 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln 675 680 685 Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 690 695 700 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 705 710 715 720 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 725 730 735 Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His 740 745 750 His His <210> 179 <211> 754 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0510.05H10(N65D) (G4S)2 S0510.10A04_stop103Q(N65D) (G4S)2 S0455.04F23(N65D) StrepII His etiqueta <400> 179 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Tyr Lys Lys Cys Gln Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Ser Glu Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 545 550 555 560 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 565 570 575 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 580 585 590 Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu Asp Lys Asp Pro 595 600 605 Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 610 615 620 Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys His Tyr Arg Ile 625 630 635 640 Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 645 650 655 Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val Arg Val Val Ser 660 665 670 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Phe Val Ile Gln 675 680 685 Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 690 695 700 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 705 710 715 720 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 725 730 735 Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His 740 745 750 His His <210> 180 <211> 754 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2-S0510.05H10(N65D) (G4S)2 S0510.10A04_stop103Q(N65D) (G4S)2-S0455.04F09(N65D) StrepII His etiqueta <400> 180 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Tyr Lys Lys Cys Gln Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Ser Glu Pro Gly Gln Thr Ser Glu Leu Val 465 470 475 480 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 485 490 495 Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr Leu Tyr Gly Arg 500 505 510 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 515 520 525 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 530 535 540 Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 545 550 555 560 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 565 570 575 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 580 585 590 Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu Asp Lys Asp Pro 595 600 605 Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 610 615 620 Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys Ala Tyr Lys Ile 625 630 635 640 Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Ala 645 650 655 Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val Arg Val Val Ser 660 665 670 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Gly Val Trp Gln 675 680 685 Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 690 695 700 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 705 710 715 720 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 725 730 735 Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys His His His His 740 745 750 His His <210> 181 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0512.17L07(N39S_N65D_T136A) (G4S)2 S0510.11H24(N65D) (G4S)2 S0455.04F23(N65D) StrepII His etiqueta <400> 181 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Ser Thr Thr Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Lys Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu 595 600 605 Asp Lys Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 182 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0512.17L07(N39S_N65D_T136A) (G4S)2-S0510.11H24(N65D) (G4S)2-S0455.04F09(N65D) StrepII His etiqueta <400> 182 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Trp 210 215 220 Ala Gly Ser Thr Thr Leu Arg Glu Asp Lys Asp Pro Pro Lys Met Pro 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Asp 245 250 255 Val Gln Phe Pro Glu Lys Lys Cys Ile Tyr Ser Thr Ile Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Gly Ile Lys Ser Ser 275 280 285 Pro Gly Gln Thr Ser His Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Tyr Val Ile Gln Asn Arg Glu Gly 305 310 315 320 Phe Asn Ile Ala Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 183 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A) (G4S)2 S0510.05H10(N65D) (G4S)2 S0510.11H24(N65D) (G4S)2 S0455.04F23(N65D) StrepII His etiqueta <400> 183 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly His Ala Gly Asn Gln Trp Leu Arg Glu 595 600 605 Asp Lys Asp Pro Arg Lys Met Trp Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr Asp Val Asp Phe Ala Ile Lys Lys Cys 625 630 635 640 His Tyr Arg Ile Thr Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Asn Ile Lys Ser His Pro Gly Gly Thr Ser Gly Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Phe Val Ile Gln Asn Arg Glu Ala Phe Phe Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 184 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65D;T54A)(G4S)2 S0510.05H10(N65D) (G4S)2 S0510.11H24(N65D) (G4S)2 S0455.04F09(N65D)StrepII His etiqueta <400> 184 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Asp Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Leu Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Ser 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Met Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Asp Val Thr Glu Val Arg Phe Arg 435 440 445 Gln Lys Lys Cys Lys Tyr Asp Ile Val Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Tyr Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Asp Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 185 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0474.01H22(N65Q;T54A) (G4S)2 S0466.09E05(N65Q) (G4S)2 S0466.13C10(N65Q) (G4S)2 S0455.04F09(N65Q) StrepII, His6 <400> 185 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Thr Ile Leu Arg Glu Asp Lys Asp Pro 35 40 45 Gly Lys Met Asn Ala Ala Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Asp Val Arg Phe Ile Arg Lys Lys Cys His Tyr Tyr Ile 65 70 75 80 Asp Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 85 90 95 Ile Lys Ser Tyr Pro Gly Thr Thr Ser Gln Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ile Val Arg Gln 115 120 125 Asn Arg Glu Ile Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755 <210> 186 <211> 758 <212> PRT <213> Secuencia Artificial <220> <223> S0475.06L06(N65Q;S79F) (G4S)2 S0466.09E05(N65Q) (G4S)2 S0466.13C10(N65Q) (G4S)2 S0455.04F09(N65Q) StrepII His etiqueta <400> 186 Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 1 5 10 15 Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 20 25 30 Val Val Gly Val Ala Gly Asn Glu Val Leu Arg Asp Asp Lys Asp Pro 35 40 45 Gly Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 50 55 60 Gln Val Thr Glu Val Arg Phe His Asn Lys Lys Cys Asn Tyr Phe Ile 65 70 75 80 Glu Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Lys 85 90 95 Ile Lys Ser Asn Pro Gly Val Thr Ser Met Leu Val Arg Val Val Ser 100 105 110 Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Asn Val Lys Gln 115 120 125 Asn Arg Glu Gly Phe Trp Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu 130 135 140 Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 145 150 155 160 Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 165 170 175 Asp Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr 180 185 190 Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln 195 200 205 Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Leu 210 215 220 Ala Gly Asn Gly Trp Leu Arg Glu Asp Lys Asp Pro Val Lys Met Met 225 230 235 240 Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Val 245 250 255 Val Asp Phe Glu Leu Lys Lys Cys Arg Tyr Met Ile Glu Thr Phe Val 260 265 270 Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asp Ile Lys Ser Phe 275 280 285 Pro Gly Trp Thr Ser Gln Leu Val Arg Val Val Ser Thr Asn Tyr Asn 290 295 300 Gln His Ala Met Val Phe Phe Lys Gly Val Tyr Gln Asn Arg Glu Trp 305 310 315 320 Phe His Ile Thr Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu 325 330 335 Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn 340 345 350 His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly 355 360 365 Gly Gly Ser Gly Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile 370 375 380 Pro Ala Pro Pro Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp 385 390 395 400 Asn Gln Phe His Gly Lys Trp Tyr Val Val Gly Glu Ala Gly Asn Leu 405 410 415 Ala Leu Arg Glu Asp Lys Asp Pro Lys Lys Met Met Ala Thr Ile Tyr 420 425 430 Glu Leu Lys Glu Asp Lys Ser Tyr Gln Val Thr Glu Val Arg Phe Arg 435 440 445 His Lys Lys Cys Gln Tyr Asp Ile Ala Thr Phe Val Pro Gly Ser Gln 450 455 460 Pro Gly Glu Phe Thr Leu Gly Leu Ile Lys Ser Asp Pro Gly Gln Thr 465 470 475 480 Ser Glu Leu Val Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met 485 490 495 Val Phe Phe Lys Lys Val Val Gln Asn Arg Glu Phe Phe Trp Ile Thr 500 505 510 Leu Tyr Gly Arg Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe 515 520 525 Ile Arg Phe Ser Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe 530 535 540 Pro Val Pro Ile Asp Gln Cys Ile Asp Gly Gly Gly Gly Gly Ser Gly 545 550 555 560 Gly Gly Gly Ser Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 565 570 575 Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 580 585 590 Gly Lys Trp Tyr Val Val Gly Met Ala Gly Asn Phe His Leu Arg Glu 595 600 605 Asp Lys Asp Pro Ser Lys Met Pro Ala Thr Ile Tyr Glu Leu Lys Glu 610 615 620 Asp Lys Ser Tyr Gln Val Thr His Val Pro Phe Trp Ala Lys Lys Cys 625 630 635 640 Ala Tyr Lys Ile Ile Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 645 650 655 Thr Leu Gly Ala Ile Lys Ser Gly Pro Gly Met Thr Ser Trp Leu Val 660 665 670 Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 675 680 685 Gly Val Trp Gln Asn Arg Glu Thr Phe Val Ile Thr Leu Tyr Gly Arg 690 695 700 Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 705 710 715 720 Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 725 730 735 Asp Gln Cys Ile Asp Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 740 745 750 His His His His His His 755