SEQUENCE LISTING <110> Malek, Thomas <120> INTERLEUKIN-2/INTERLEUKIN-2 RECEPTOR ALPHA FUSION PROTEINS AND METHODS OF USE <130> 3338.097PC01/ELE/C-K/MKK <150> US 62/033,726 <151> 2014-08-06 <150> PCT/US2015/043792 <151> 2015-08-05 <160> 65 <170> PatentIn version 3.5 <210> 1 <211> 153 <212> PRT <213> Homo sapeins <400> 1 Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr 145 150 <210> 2 <211> 133 <212> PRT <213> Homo sapiens <400> 2 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr 130 <210> 3 <211> 169 <212> PRT <213> Mus musculus <400> 3 Met Tyr Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln 165 <210> 4 <211> 149 <212> PRT <213> Mus musculus <400> 4 Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 1 5 10 15 Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 20 25 30 Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 35 40 45 Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 50 55 60 Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 65 70 75 80 Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 85 90 95 Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 100 105 110 Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 115 120 125 Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 130 135 140 Ser Thr Ser Pro Gln 145 <210> 5 <211> 272 <212> PRT <213> Homo sapiens <400> 5 Met Asp Ser Tyr Leu Leu Met Trp Gly Leu Leu Thr Phe Ile Met Val 1 5 10 15 Pro Gly Cys Gln Ala Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 20 25 30 His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 35 40 45 Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 50 55 60 Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 65 70 75 80 Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 85 90 95 Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 100 105 110 Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 115 120 125 Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 130 135 140 Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 145 150 155 160 Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 165 170 175 Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 180 185 190 Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 195 200 205 Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 210 215 220 Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 225 230 235 240 Val Ala Val Ala Gly Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu 245 250 255 Ser Gly Leu Thr Trp Gln Arg Arg Gln Arg Lys Ser Arg Arg Thr Ile 260 265 270 <210> 6 <211> 251 <212> PRT <213> Homo sapiens <400> 6 Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys 1 5 10 15 Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg 20 25 30 Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly 35 40 45 Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser 50 55 60 Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln 65 70 75 80 Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp 85 90 95 Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn 100 105 110 Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr 115 120 125 Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu 130 135 140 Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln 145 150 155 160 Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu 165 170 175 Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys 180 185 190 Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr 195 200 205 Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln Val Ala Val Ala Gly 210 215 220 Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu Ser Gly Leu Thr Trp 225 230 235 240 Gln Arg Arg Gln Arg Lys Ser Arg Arg Thr Ile 245 250 <210> 7 <211> 219 <212> PRT <213> Homo sapiens <400> 7 Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys 1 5 10 15 Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg 20 25 30 Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly 35 40 45 Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser 50 55 60 Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln 65 70 75 80 Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp 85 90 95 Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn 100 105 110 Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr 115 120 125 Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu 130 135 140 Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln 145 150 155 160 Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu 165 170 175 Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys 180 185 190 Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr 195 200 205 Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 210 215 <210> 8 <211> 268 <212> PRT <213> Mus musculus <400> 8 Met Glu Pro Arg Leu Leu Met Leu Gly Phe Leu Ser Leu Thr Ile Val 1 5 10 15 Pro Ser Cys Arg Ala Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 20 25 30 Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 35 40 45 Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 50 55 60 Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 65 70 75 80 Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 85 90 95 Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 100 105 110 His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 115 120 125 His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 130 135 140 His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 145 150 155 160 Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 165 170 175 Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 180 185 190 Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 195 200 205 Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 210 215 220 Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys Val Ala Val Ala 225 230 235 240 Ser Cys Leu Phe Leu Leu Ile Ser Ile Leu Leu Leu Ser Gly Leu Thr 245 250 255 Trp Gln His Arg Trp Arg Lys Ser Arg Arg Thr Ile 260 265 <210> 9 <211> 247 <212> PRT <213> Mus musculus <400> 9 Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr Phe Lys 1 5 10 15 Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys Lys Arg 20 25 30 Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu Gly Asn 35 40 45 Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp Lys Ser 50 55 60 Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln Gln Thr 65 70 75 80 Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met His Gln Glu Asn Leu 85 90 95 Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp Ser Lys 100 105 110 Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val His Tyr Glu Cys Ile 115 120 125 Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile Cys Lys 130 135 140 Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr Cys Val 145 150 155 160 Asp Glu Arg Glu His His Arg Phe Leu Ala Ser Glu Glu Ser Gln Gly 165 170 175 Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile Thr Thr 180 185 190 Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu Thr Phe 195 200 205 Val Leu Thr Met Glu Tyr Lys Val Ala Val Ala Ser Cys Leu Phe Leu 210 215 220 Leu Ile Ser Ile Leu Leu Leu Ser Gly Leu Thr Trp Gln His Arg Trp 225 230 235 240 Arg Lys Ser Arg Arg Thr Ile 245 <210> 10 <211> 215 <212> PRT <213> Mus musculus <400> 10 Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr Phe Lys 1 5 10 15 Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys Lys Arg 20 25 30 Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu Gly Asn 35 40 45 Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp Lys Ser 50 55 60 Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln Gln Thr 65 70 75 80 Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met His Gln Glu Asn Leu 85 90 95 Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp Ser Lys 100 105 110 Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val His Tyr Glu Cys Ile 115 120 125 Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile Cys Lys 130 135 140 Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr Cys Val 145 150 155 160 Asp Glu Arg Glu His His Arg Phe Leu Ala Ser Glu Glu Ser Gln Gly 165 170 175 Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile Thr Thr 180 185 190 Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu Thr Phe 195 200 205 Val Leu Thr Met Glu Tyr Lys 210 215 <210> 11 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> (Gly3Ser)4 linker <400> 11 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 1 5 10 15 <210> 12 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> (Gly3Ser)2 linker <400> 12 Gly Gly Gly Ser Gly Gly Gly Ser 1 5 <210> 13 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> (Gly3Ser)3 linker <400> 13 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 1 5 10 <210> 14 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> (Gly3Ser)5 linker <400> 14 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 1 5 10 15 Gly Gly Gly Ser 20 <210> 15 <211> 3 <212> PRT <213> Artificial Sequence <220> <223> Gly3 linker <400> 15 Gly Gly Gly 1 <210> 16 <211> 384 <212> PRT <213> Artificial Sequence <220> <223> Synthesized Mature form of IL-2 (Gly4Ser)4-extracellular domain of IL-2 R <400> 16 Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 1 5 10 15 Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 20 25 30 Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 35 40 45 Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 50 55 60 Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 65 70 75 80 Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 85 90 95 Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 100 105 110 Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 115 120 125 Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 130 135 140 Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 145 150 155 160 Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro 165 170 175 Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly 180 185 190 Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu 195 200 205 Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln 210 215 220 Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln 225 230 235 240 Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro 245 250 255 Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro 260 265 270 Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu 275 280 285 Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln 290 295 300 Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly 305 310 315 320 Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg 325 330 335 Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu 340 345 350 Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr 355 360 365 Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 370 375 380 <210> 17 <211> 404 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)4-extracellular domain of IL-2 R <400> 17 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly 165 170 175 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys 180 185 190 Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu Ser 195 200 205 Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg 210 215 220 Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp Ser 225 230 235 240 Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys Gln 245 250 255 Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr Asp 260 265 270 Met Gln Lys Pro Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly His 275 280 285 Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile Tyr 290 295 300 His Phe Val Glu Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly Tyr 305 310 315 320 Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys Cys 325 330 335 Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu Arg 340 345 350 Glu His His Arg Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg Asn 355 360 365 Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp Phe 370 375 380 Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu Thr 385 390 395 400 Met Glu Tyr Lys <210> 18 <211> 389 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form of IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 18 Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 1 5 10 15 Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 20 25 30 Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 35 40 45 Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 50 55 60 Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 65 70 75 80 Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 85 90 95 Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 100 105 110 Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 115 120 125 Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 130 135 140 Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 145 150 155 160 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 165 170 175 Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu 180 185 190 Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe 195 200 205 Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp 210 215 220 Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys 225 230 235 240 Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr 245 250 255 Asp Met Gln Lys Pro Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly 260 265 270 His Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile 275 280 285 Tyr His Phe Val Glu Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly 290 295 300 Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys 305 310 315 320 Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu 325 330 335 Arg Glu His His Arg Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg 340 345 350 Asn Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp 355 360 365 Phe Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu 370 375 380 Thr Met Glu Tyr Lys 385 <210> 19 <211> 409 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 19 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly 165 170 175 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 180 185 190 Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr 195 200 205 Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys 210 215 220 Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu 225 230 235 240 Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp 245 250 255 Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln 260 265 270 Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met His Gln Glu 275 280 285 Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp 290 295 300 Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val His Tyr Glu 305 310 315 320 Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile 325 330 335 Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr 340 345 350 Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser Glu Glu Ser 355 360 365 Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile 370 375 380 Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu 385 390 395 400 Thr Phe Val Leu Thr Met Glu Tyr Lys 405 <210> 20 <211> 380 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form of IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 20 Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 1 5 10 15 Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 20 25 30 Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 35 40 45 Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 50 55 60 Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 65 70 75 80 Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 85 90 95 Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 100 105 110 Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 115 120 125 Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 130 135 140 Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 165 170 175 Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 180 185 190 Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 195 200 205 Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 210 215 220 Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 225 230 235 240 Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 245 250 255 His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 260 265 270 His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 275 280 285 His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 290 295 300 Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 305 310 315 320 Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 325 330 335 Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 340 345 350 Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 355 360 365 Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 370 375 380 <210> 21 <211> 400 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 21 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly 165 170 175 Ser Gly Gly Gly Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro 180 185 190 Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly 195 200 205 Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu 210 215 220 Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln 225 230 235 240 Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln 245 250 255 Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro 260 265 270 Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro 275 280 285 Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu 290 295 300 Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln 305 310 315 320 Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly 325 330 335 Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg 340 345 350 Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu 355 360 365 Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr 370 375 380 Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 385 390 395 400 <210> 22 <211> 372 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly4Ser)4- extracellular domain of IL-2 R <400> 22 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 130 135 140 Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro 145 150 155 160 Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly 165 170 175 Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser 180 185 190 Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp 195 200 205 Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys 210 215 220 Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu 225 230 235 240 Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His 245 250 255 Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr 260 265 270 His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr 275 280 285 Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His 290 295 300 Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met 305 310 315 320 Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu 325 330 335 Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe 340 345 350 Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr 355 360 365 Thr Glu Tyr Gln 370 <210> 23 <211> 392 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly4Ser)4- extracellular domain of IL-2 R <400> 23 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly 145 150 155 160 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys 165 170 175 Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala 180 185 190 Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg 195 200 205 Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser 210 215 220 His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg 225 230 235 240 Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg 245 250 255 Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser 260 265 270 Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr 275 280 285 Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys 290 295 300 Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys 305 310 315 320 Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys 325 330 335 Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln 340 345 350 Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr 355 360 365 Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr 370 375 380 Ser Ile Phe Thr Thr Glu Tyr Gln 385 390 <210> 24 <211> 368 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 24 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 130 135 140 Ser Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 145 150 155 160 His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 165 170 175 Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 180 185 190 Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 195 200 205 Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 210 215 220 Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 225 230 235 240 Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 245 250 255 Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 260 265 270 Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 275 280 285 Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 290 295 300 Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 305 310 315 320 Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 325 330 335 Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 340 345 350 Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 355 360 365 <210> 25 <211> 388 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 25 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Gly Gly Gly Ser Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro 165 170 175 Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly 180 185 190 Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser 195 200 205 Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp 210 215 220 Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys 225 230 235 240 Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu 245 250 255 Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His 260 265 270 Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr 275 280 285 His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr 290 295 300 Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His 305 310 315 320 Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met 325 330 335 Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu 340 345 350 Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe 355 360 365 Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr 370 375 380 Thr Glu Tyr Gln 385 <210> 26 <211> 364 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 26 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 130 135 140 Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe 145 150 155 160 Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys 165 170 175 Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr 180 185 190 Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser 195 200 205 Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu 210 215 220 Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val 225 230 235 240 Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu 245 250 255 Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val 260 265 270 Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala 275 280 285 Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro 290 295 300 Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu 305 310 315 320 Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser 325 330 335 Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala 340 345 350 Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 355 360 <210> 27 <211> 384 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 27 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 165 170 175 His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 180 185 190 Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 195 200 205 Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 210 215 220 Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 225 230 235 240 Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 245 250 255 Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 260 265 270 Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 275 280 285 Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 290 295 300 Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 305 310 315 320 Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 325 330 335 Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 340 345 350 Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 355 360 365 Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 370 375 380 <210> 28 <211> 66 <212> DNA <213> Artificial Sequence <220> <223> IL-2 leader optimized Kozak sequence <400> 28 gccaccatgg acaggatgca actcctgtct tgcattgcac taagtcttgc acttgtcaca 60 aacagt 66 <210> 29 <211> 1239 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)4-extracellular domain of IL-2 R <400> 29 atggacagca tgcagctcgc atcctgtgtc acattgacac ttgtgctcct tgtcaacagc 60 gcacccactt caagctctac ttcaagctct acagcggaag cacagcagca gcagcagcag 120 cagcagcagc agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg 180 atggagaatt acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc 240 aagcaggcca cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg 300 catgttctgg atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc 360 agcaatatca gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa 420 ttcgatgatg agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa 480 agcatcatct caacaagccc tcaaggtgga ggtggatctg gtggaggtgg atcaggtgga 540 ggtggatccg gtggaggtgg atctgaactg tgtctgtatg acccacccga ggtccccaat 600 gccacattca aagccctctc ctacaagaac ggcaccatcc taaactgtga atgcaagaga 660 ggtttccgaa gactaaagga attggtctat atgcgttgct taggaaactc ctggagcagc 720 aactgccagt gcaccagcaa ctcccatgac aaatcgagaa agcaagttac agctcaactt 780 gaacaccaga aagagcaaca aaccacaaca gacatgcaga agccaacaca gtctatgcac 840 caagagaacc ttacaggtca ctgcagggag ccacctcctt ggaaacatga agattccaag 900 agaatctatc atttcgtgga aggacagagt gttcactacg agtgtattcc gggatacaag 960 gctctacaga gaggtcctgc tattagcatc tgcaagatga agtgtgggaa aacggggtgg 1020 actcagcccc agctcacatg tgtagatgaa agagaacacc accgatttct ggctagtgag 1080 gaatctcaag gaagcagaaa ttcttctccc gagagtgaga cttcctgccc cataaccacc 1140 acagacttcc cacaacccac agaaacaact gcaatgacgg agacatttgt gctcacaatg 1200 gagtataagg gtggacatca ccatcaccat cactaataa 1239 <210> 30 <211> 1227 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 30 atggacagca tgcagctcgc atcctgtgtc acattgacac ttgtgctcct tgtcaacagc 60 gcacccactt caagctctac ttcaagctct acagcggaag cacagcagca gcagcagcag 120 cagcagcagc agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg 180 atggagaatt acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc 240 aagcaggcca cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg 300 catgttctgg atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc 360 agcaatatca gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa 420 ttcgatgatg agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa 480 agcatcatct caacaagccc tcaaggtgga ggttctggtg gaggttcagg tggaggttcg 540 ggtggaggtt ctgaactgtg tctgtatgac ccacccgagg tccccaatgc cacattcaaa 600 gccctctcct acaagaacgg caccatccta aactgtgaat gcaagagagg tttccgaaga 660 ctaaaggaat tggtctatat gcgttgctta ggaaactcct ggagcagcaa ctgccagtgc 720 accagcaact cccatgacaa atcgagaaag caagttacag ctcaacttga acaccagaaa 780 gagcaacaaa ccacaacaga catgcagaag ccaacacagt ctatgcacca agagaacctt 840 acaggtcact gcagggagcc acctccttgg aaacatgaag attccaagag aatctatcat 900 ttcgtggaag gacagagtgt tcactacgag tgtattccgg gatacaaggc tctacagaga 960 ggtcctgcta ttagcatctg caagatgaag tgtgggaaaa cggggtggac tcagccccag 1020 ctcacatgtg tagatgaaag agaacaccac cgatttctgg ctagtgagga atctcaagga 1080 agcagaaatt cttctcccga gagtgagact tcctgcccca taaccaccac agacttccca 1140 caacccacag aaacaactgc aatgacggag acatttgtgc tcacaatgga gtataagggt 1200 ggacatcacc atcaccatca ctaataa 1227 <210> 31 <211> 1254 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 31 atggacagca tgcagctcgc atcctgtgtc acattgacac ttgtgctcct tgtcaacagc 60 gcacccactt caagctctac ttcaagctct acagcggaag cacagcagca gcagcagcag 120 cagcagcagc agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg 180 atggagaatt acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc 240 aagcaggcca cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg 300 catgttctgg atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc 360 agcaatatca gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa 420 ttcgatgatg agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa 480 agcatcatct caacaagccc tcaaggtgga ggtggatcag gtggaggtgg atctggtgga 540 ggtggatcag gtggaggtgg atccggtgga ggtggatctg aactgtgtct gtatgaccca 600 cccgaggtcc ccaatgccac attcaaagcc ctctcctaca agaacggcac catcctaaac 660 tgtgaatgca agagaggttt ccgaagacta aaggaattgg tctatatgcg ttgcttagga 720 aactcctgga gcagcaactg ccagtgcacc agcaactccc atgacaaatc gagaaagcaa 780 gttacagctc aacttgaaca ccagaaagag caacaaacca caacagacat gcagaagcca 840 acacagtcta tgcaccaaga gaaccttaca ggtcactgca gggagccacc tccttggaaa 900 catgaagatt ccaagagaat ctatcatttc gtggaaggac agagtgttca ctacgagtgt 960 attccgggat acaaggctct acagagaggt cctgctatta gcatctgcaa gatgaagtgt 1020 gggaaaacgg ggtggactca gccccagctc acatgtgtag atgaaagaga acaccaccga 1080 tttctggcta gtgaggaatc tcaaggaagc agaaattctt ctcccgagag tgagacttcc 1140 tgccccataa ccaccacaga cttcccacaa cccacagaaa caactgcaat gacggagaca 1200 tttgtgctca caatggagta taagggtgga catcaccatc accatcacta ataa 1254 <210> 32 <211> 1206 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly4Ser)4- extracellular domain of IL-2 R <400> 32 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggtgg atctggtgga 480 ggtggatcag gtggaggtgg atccggtgga ggtggatctg agctctgtga cgatgacccg 540 ccagagatcc cacacgccac attcaaagcc atggcctaca aggaaggaac catgttgaac 600 tgtgaatgca agagaggttt ccgcagaata aaaagcgggt cactctatat gctctgtaca 660 ggaaactcta gccactcgtc ctgggacaac caatgtcaat gcacaagctc tgccactcgg 720 aacacaacga aacaagtgac acctcaacct gaagaacaga aagaaaggaa aaccacagaa 780 atgcaaagtc caatgcagcc agtggaccaa gcgagccttc caggtcactg cagggaacct 840 ccaccatggg aaaatgaagc cacagagaga atttatcatt tcgtggtggg gcagatggtt 900 tattatcagt gcgtccaggg atacagggct ctacacagag gtcctgctga gagcgtctgc 960 aaaatgaccc acgggaagac aaggtggacc cagccccagc tcatatgcac aggtgaaatg 1020 gagaccagtc agtttccagg tgaagagaag cctcaggcaa gccccgaagg ccgtcctgag 1080 agtgagactt cctgcctcgt cacaacaaca gattttcaaa tacagacaga aatggctgca 1140 accatggaga cgtccatatt tacaacagag taccagggtg gacatcacca tcaccatcac 1200 taataa 1206 <210> 33 <211> 1194 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)4- extracellular domain of IL-2 R <400> 33 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggttc tggtggaggt 480 tcaggtggag gttcgggtgg aggttctgag ctctgtgacg atgacccgcc agagatccca 540 cacgccacat tcaaagccat ggcctacaag gaaggaacca tgttgaactg tgaatgcaag 600 agaggtttcc gcagaataaa aagcgggtca ctctatatgc tctgtacagg aaactctagc 660 cactcgtcct gggacaacca atgtcaatgc acaagctctg ccactcggaa cacaacgaaa 720 caagtgacac ctcaacctga agaacagaaa gaaaggaaaa ccacagaaat gcaaagtcca 780 atgcagccag tggaccaagc gagccttcca ggtcactgca gggaacctcc accatgggaa 840 aatgaagcca cagagagaat ttatcatttc gtggtggggc agatggttta ttatcagtgc 900 gtccagggat acagggctct acacagaggt cctgctgaga gcgtctgcaa aatgacccac 960 gggaagacaa ggtggaccca gccccagctc atatgcacag gtgaaatgga gaccagtcag 1020 tttccaggtg aagagaagcc tcaggcaagc cccgaaggcc gtcctgagag tgagacttcc 1080 tgcctcgtca caacaacaga ttttcaaata cagacagaaa tggctgcaac catggagacg 1140 tccatattta caacagagta ccagggtgga catcaccatc accatcacta ataa 1194 <210> 34 <211> 1182 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 34 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggttc tggtggaggt 480 tcaggtggag gttcggagct ctgtgacgat gacccgccag agatcccaca cgccacattc 540 aaagccatgg cctacaagga aggaaccatg ttgaactgtg aatgcaagag aggtttccgc 600 agaataaaaa gcgggtcact ctatatgctc tgtacaggaa actctagcca ctcgtcctgg 660 gacaaccaat gtcaatgcac aagctctgcc actcggaaca caacgaaaca agtgacacct 720 caacctgaag aacagaaaga aaggaaaacc acagaaatgc aaagtccaat gcagccagtg 780 gaccaagcga gccttccagg tcactgcagg gaacctccac catgggaaaa tgaagccaca 840 gagagaattt atcatttcgt ggtggggcag atggtttatt atcagtgcgt ccagggatac 900 agggctctac acagaggtcc tgctgagagc gtctgcaaaa tgacccacgg gaagacaagg 960 tggacccagc cccagctcat atgcacaggt gaaatggaga ccagtcagtt tccaggtgaa 1020 gagaagcctc aggcaagccc cgaaggccgt cctgagagtg agacttcctg cctcgtcaca 1080 acaacagatt ttcaaataca gacagaaatg gctgcaacca tggagacgtc catatttaca 1140 acagagtacc agggtggaca tcaccatcac catcactaat aa 1182 <210> 35 <211> 13 <212> DNA <213> Artificial Sequence <220> <223> Kozak consensus <400> 35 gccgccrcca ugg 13 <210> 36 <211> 396 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 36 Met Tyr Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly 165 170 175 Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 180 185 190 Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 195 200 205 Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 210 215 220 Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 225 230 235 240 Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 245 250 255 Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 260 265 270 His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 275 280 285 His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 290 295 300 His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 305 310 315 320 Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 325 330 335 Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 340 345 350 Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 355 360 365 Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 370 375 380 Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 385 390 395 <210> 37 <211> 396 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 37 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly 165 170 175 Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 180 185 190 Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 195 200 205 Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 210 215 220 Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 225 230 235 240 Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 245 250 255 Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 260 265 270 His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 275 280 285 His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 290 295 300 His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 305 310 315 320 Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 325 330 335 Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 340 345 350 Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 355 360 365 Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 370 375 380 Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 385 390 395 <210> 38 <211> 397 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 38 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly 145 150 155 160 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 165 170 175 Gly Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr 180 185 190 Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys 195 200 205 Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys 210 215 220 Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr 225 230 235 240 Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu 245 250 255 Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro 260 265 270 Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp 275 280 285 Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met 290 295 300 Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro 305 310 315 320 Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln 325 330 335 Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly 340 345 350 Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr 355 360 365 Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala 370 375 380 Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 385 390 395 <210> 39 <211> 377 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form of human IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 39 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 130 135 140 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu 145 150 155 160 Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met 165 170 175 Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe 180 185 190 Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser 195 200 205 Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr 210 215 220 Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu 225 230 235 240 Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala 245 250 255 Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala 260 265 270 Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr Tyr Gln 275 280 285 Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val 290 295 300 Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile 305 310 315 320 Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro 325 330 335 Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val 340 345 350 Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu 355 360 365 Thr Ser Ile Phe Thr Thr Glu Tyr Gln 370 375 <210> 40 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> Linker sequence (Gly4Ser)4 <400> 40 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 Gly Gly Gly Ser 20 <210> 41 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> Linker sequence (Gly4Ser)5 <400> 41 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 Gly Gly Gly Ser Gly Gly Gly Gly Ser 20 25 <210> 42 <211> 1215 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)3- extracellular domain of IL-2 R <400> 42 atggacagca tgcagctcgc atcctgtgtc acattgacac ttgtgctcct tgtcaacagc 60 gcacccactt caagctctac ttcaagctct acagcggaag cacagcagca gcagcagcag 120 cagcagcagc agcagcacct ggagcagctg ttgatggacc tacaggagct cctgagcagg 180 atggagaatt acaggaacct gaaactcccc aggatgctca ccttcaaatt ttacttgccc 240 aagcaggcca cagaattgaa agatcttcag tgcctagaag atgaacttgg acctctgcgg 300 catgttctgg atttgactca aagcaaaagc tttcaattgg aagatgctga gaatttcatc 360 agcaatatca gagtaactgt tgtaaaacta aagggctctg acaacacatt tgagtgccaa 420 ttcgatgatg agtcagcaac tgtggtggac tttctgagga gatggatagc cttctgtcaa 480 agcatcatct caacaagccc tcaaggtgga ggttctggtg gaggttcagg tggaggttcg 540 gaactgtgtc tgtatgaccc acccgaggtc cccaatgcca cattcaaagc cctctcctac 600 aagaacggca ccatcctaaa ctgtgaatgc aagagaggtt tccgaagact aaaggaattg 660 gtctatatgc gttgcttagg aaactcctgg agcagcaact gccagtgcac cagcaactcc 720 catgacaaat cgagaaagca agttacagct caacttgaac accagaaaga gcaacaaacc 780 acaacagaca tgcagaagcc aacacagtct atgcaccaag agaaccttac aggtcactgc 840 agggagccac ctccttggaa acatgaagat tccaagagaa tctatcattt cgtggaagga 900 cagagtgttc actacgagtg tattccggga tacaaggctc tacagagagg tcctgctatt 960 agcatctgca agatgaagtg tgggaaaacg gggtggactc agccccagct cacatgtgta 1020 gatgaaagag aacaccaccg atttctggct agtgaggaat ctcaaggaag cagaaattct 1080 tctcccgaga gtgagacttc ctgccccata accaccacag acttcccaca acccacagaa 1140 acaactgcaa tgacggagac atttgtgctc acaatggagt ataagggtgg acatcaccat 1200 caccatcact aataa 1215 <210> 43 <211> 360 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly3Ser)2- extracellular domain of IL-2 R <400> 43 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly Ser Glu Leu Cys 130 135 140 Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala 145 150 155 160 Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg 165 170 175 Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser 180 185 190 His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg 195 200 205 Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg 210 215 220 Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser 225 230 235 240 Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr 245 250 255 Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys 260 265 270 Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys 275 280 285 Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys 290 295 300 Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln 305 310 315 320 Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr 325 330 335 Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr 340 345 350 Ser Ile Phe Thr Thr Glu Tyr Gln 355 360 <210> 44 <211> 380 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)2- extracellular domain of IL-2 R <400> 44 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe 165 170 175 Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys 180 185 190 Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr 195 200 205 Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser 210 215 220 Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu 225 230 235 240 Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val 245 250 255 Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu 260 265 270 Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val 275 280 285 Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala 290 295 300 Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro 305 310 315 320 Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu 325 330 335 Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser 340 345 350 Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala 355 360 365 Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 370 375 380 <210> 45 <211> 355 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly3)- extracellular domain of IL-2 R <400> 45 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Glu Leu Cys Asp Asp Asp Pro Pro 130 135 140 Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr 145 150 155 160 Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly 165 170 175 Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp 180 185 190 Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln 195 200 205 Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met 210 215 220 Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys 225 230 235 240 Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His 245 250 255 Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg 260 265 270 Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly 275 280 285 Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu 290 295 300 Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly 305 310 315 320 Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln 325 330 335 Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr 340 345 350 Glu Tyr Gln 355 <210> 46 <211> 375 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3)- extracellular domain of IL-2 R <400> 46 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Glu Leu Cys Asp 145 150 155 160 Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr 165 170 175 Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg 180 185 190 Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His 195 200 205 Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn 210 215 220 Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys 225 230 235 240 Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu 245 250 255 Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu 260 265 270 Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val 275 280 285 Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys 290 295 300 Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr 305 310 315 320 Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala 325 330 335 Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr 340 345 350 Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser 355 360 365 Ile Phe Thr Thr Glu Tyr Gln 370 375 <210> 47 <211> 1170 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)2- extracellular domain of IL-2 R <400> 47 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggttc tggtggaggt 480 tcagagctct gtgacgatga cccgccagag atcccacacg ccacattcaa agccatggcc 540 tacaaggaag gaaccatgtt gaactgtgaa tgcaagagag gtttccgcag aataaaaagc 600 gggtcactct atatgctctg tacaggaaac tctagccact cgtcctggga caaccaatgt 660 caatgcacaa gctctgccac tcggaacaca acgaaacaag tgacacctca acctgaagaa 720 cagaaagaaa ggaaaaccac agaaatgcaa agtccaatgc agccagtgga ccaagcgagc 780 cttccaggtc actgcaggga acctccacca tgggaaaatg aagccacaga gagaatttat 840 catttcgtgg tggggcagat ggtttattat cagtgcgtcc agggatacag ggctctacac 900 agaggtcctg ctgagagcgt ctgcaaaatg acccacggga agacaaggtg gacccagccc 960 cagctcatat gcacaggtga aatggagacc agtcagtttc caggtgaaga gaagcctcag 1020 gcaagccccg aaggccgtcc tgagagtgag acttcctgcc tcgtcacaac aacagatttt 1080 caaatacaga cagaaatggc tgcaaccatg gagacgtcca tatttacaac agagtaccag 1140 ggtggacatc accatcacca tcactaataa 1170 <210> 48 <211> 1155 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3)- extracellular domain of IL-2 R <400> 48 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggtga gctctgtgac 480 gatgacccgc cagagatccc acacgccaca ttcaaagcca tggcctacaa ggaaggaacc 540 atgttgaact gtgaatgcaa gagaggtttc cgcagaataa aaagcgggtc actctatatg 600 ctctgtacag gaaactctag ccactcgtcc tgggacaacc aatgtcaatg cacaagctct 660 gccactcgga acacaacgaa acaagtgaca cctcaacctg aagaacagaa agaaaggaaa 720 accacagaaa tgcaaagtcc aatgcagcca gtggaccaag cgagccttcc aggtcactgc 780 agggaacctc caccatggga aaatgaagcc acagagagaa tttatcattt cgtggtgggg 840 cagatggttt attatcagtg cgtccaggga tacagggctc tacacagagg tcctgctgag 900 agcgtctgca aaatgaccca cgggaagaca aggtggaccc agccccagct catatgcaca 960 ggtgaaatgg agaccagtca gtttccaggt gaagagaagc ctcaggcaag ccccgaaggc 1020 cgtcctgaga gtgagacttc ctgcctcgtc acaacaacag attttcaaat acagacagaa 1080 atggctgcaa ccatggagac gtccatattt acaacagagt accagggtgg acatcaccat 1140 caccatcact aataa 1155 <210> 49 <211> 1221 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly4Ser)5- extracellular domain of IL-2 R <400> 49 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggtgg atcaggtgga 480 ggtggatctg gtggaggtgg atcaggtgga ggtggatccg gtggaggtgg atctgagctc 540 tgtgacgatg acccgccaga gatcccacac gccacattca aagccatggc ctacaaggaa 600 ggaaccatgt tgaactgtga atgcaagaga ggtttccgca gaataaaaag cgggtcactc 660 tatatgctct gtacaggaaa ctctagccac tcgtcctggg acaaccaatg tcaatgcaca 720 agctctgcca ctcggaacac aacgaaacaa gtgacacctc aacctgaaga acagaaagaa 780 aggaaaacca cagaaatgca aagtccaatg cagccagtgg accaagcgag ccttccaggt 840 cactgcaggg aacctccacc atgggaaaat gaagccacag agagaattta tcatttcgtg 900 gtggggcaga tggtttatta tcagtgcgtc cagggataca gggctctaca cagaggtcct 960 gctgagagcg tctgcaaaat gacccacggg aagacaaggt ggacccagcc ccagctcata 1020 tgcacaggtg aaatggagac cagtcagttt ccaggtgaag agaagcctca ggcaagcccc 1080 gaaggccgtc ctgagagtga gacttcctgc ctcgtcacaa caacagattt tcaaatacag 1140 acagaaatgg ctgcaaccat ggagacgtcc atatttacaa cagagtacca gggtggacat 1200 caccatcacc atcactaata a 1221 <210> 50 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> (Gly4Ser)3 linker <400> 50 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 15 <210> 51 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> (Gly4Ser)2 linker <400> 51 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 <210> 52 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> (Gly4Ser)1 linker <400> 52 Gly Gly Gly Gly Ser 1 5 <210> 53 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> Kozak sequence <400> 53 gccaccatgg 10 <210> 54 <211> 412 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)4-extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 54 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly 165 170 175 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys 180 185 190 Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu Ser 195 200 205 Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg 210 215 220 Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp Ser 225 230 235 240 Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys Gln 245 250 255 Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr Asp 260 265 270 Met Gln Lys Pro Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly His 275 280 285 Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile Tyr 290 295 300 His Phe Val Glu Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly Tyr 305 310 315 320 Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys Cys 325 330 335 Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu Arg 340 345 350 Glu His His Arg Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg Asn 355 360 365 Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp Phe 370 375 380 Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu Thr 385 390 395 400 Met Glu Tyr Lys Gly Gly His His His His His His 405 410 <210> 55 <211> 417 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly4Ser)5- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 55 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly 165 170 175 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 180 185 190 Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro Asn Ala Thr 195 200 205 Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn Cys Glu Cys 210 215 220 Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met Arg Cys Leu 225 230 235 240 Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn Ser His Asp 245 250 255 Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln Lys Glu Gln 260 265 270 Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met His Gln Glu 275 280 285 Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys His Glu Asp 290 295 300 Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val His Tyr Glu 305 310 315 320 Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala Ile Ser Ile 325 330 335 Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro Gln Leu Thr 340 345 350 Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser Glu Glu Ser 355 360 365 Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser Cys Pro Ile 370 375 380 Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala Met Thr Glu 385 390 395 400 Thr Phe Val Leu Thr Met Glu Tyr Lys Gly Gly His His His His His 405 410 415 His <210> 56 <211> 408 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)4- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 56 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly 165 170 175 Ser Gly Gly Gly Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro 180 185 190 Pro Glu Val Pro Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly 195 200 205 Thr Ile Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu 210 215 220 Leu Val Tyr Met Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln 225 230 235 240 Cys Thr Ser Asn Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln 245 250 255 Leu Glu His Gln Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro 260 265 270 Thr Gln Ser Met His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro 275 280 285 Pro Pro Trp Lys His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu 290 295 300 Gly Gln Ser Val His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln 305 310 315 320 Arg Gly Pro Ala Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly 325 330 335 Trp Thr Gln Pro Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg 340 345 350 Phe Leu Ala Ser Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu 355 360 365 Ser Glu Thr Ser Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr 370 375 380 Glu Thr Thr Ala Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys 385 390 395 400 Gly Gly His His His His His His 405 <210> 57 <211> 404 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 57 Met Asp Ser Met Gln Leu Ala Ser Cys Val Thr Leu Thr Leu Val Leu 1 5 10 15 Leu Val Asn Ser Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala 20 25 30 Glu Ala Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu 35 40 45 Glu Gln Leu Leu Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn 50 55 60 Tyr Arg Asn Leu Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu 65 70 75 80 Pro Lys Gln Ala Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu 85 90 95 Leu Gly Pro Leu Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe 100 105 110 Gln Leu Glu Asp Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val 115 120 125 Val Lys Leu Lys Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp 130 135 140 Glu Ser Ala Thr Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys 145 150 155 160 Gln Ser Ile Ile Ser Thr Ser Pro Gln Gly Gly Gly Ser Gly Gly Gly 165 170 175 Ser Gly Gly Gly Ser Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 180 185 190 Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 195 200 205 Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 210 215 220 Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 225 230 235 240 Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 245 250 255 Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 260 265 270 His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 275 280 285 His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 290 295 300 His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 305 310 315 320 Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 325 330 335 Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 340 345 350 Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 355 360 365 Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 370 375 380 Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys Gly Gly His His 385 390 395 400 His His His His <210> 58 <211> 388 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)2- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 58 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe 165 170 175 Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys 180 185 190 Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr 195 200 205 Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser 210 215 220 Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu 225 230 235 240 Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val 245 250 255 Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu 260 265 270 Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val 275 280 285 Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala 290 295 300 Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro 305 310 315 320 Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu 325 330 335 Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser 340 345 350 Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala 355 360 365 Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln Gly Gly His His 370 375 380 His His His His 385 <210> 59 <211> 392 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form of IL-2 (Gly3Ser)3- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 59 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 165 170 175 His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 180 185 190 Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 195 200 205 Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 210 215 220 Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 225 230 235 240 Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 245 250 255 Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 260 265 270 Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 275 280 285 Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 290 295 300 Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 305 310 315 320 Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 325 330 335 Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 340 345 350 Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 355 360 365 Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 370 375 380 Gly Gly His His His His His His 385 390 <210> 60 <211> 396 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)4- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 60 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly 145 150 155 160 Ser Gly Gly Gly Ser Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro 165 170 175 Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly 180 185 190 Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser 195 200 205 Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp 210 215 220 Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys 225 230 235 240 Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu 245 250 255 Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His 260 265 270 Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr 275 280 285 His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr 290 295 300 Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His 305 310 315 320 Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met 325 330 335 Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu 340 345 350 Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe 355 360 365 Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr 370 375 380 Thr Glu Tyr Gln Gly Gly His His His His His His 385 390 395 <210> 61 <211> 400 <212> PRT <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly4Ser)4- extracellular domain of IL-2 R + glycine spacer and poly-histidine region <400> 61 Met Asp Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 1 5 10 15 Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 20 25 30 Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 35 40 45 Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 50 55 60 Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 65 70 75 80 Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 85 90 95 Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 100 105 110 Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 115 120 125 Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 130 135 140 Cys Gln Ser Ile Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly 145 150 155 160 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys 165 170 175 Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala 180 185 190 Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Arg 195 200 205 Arg Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser 210 215 220 His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg 225 230 235 240 Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg 245 250 255 Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser 260 265 270 Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr 275 280 285 Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys 290 295 300 Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys 305 310 315 320 Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys 325 330 335 Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln 340 345 350 Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr 355 360 365 Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr 370 375 380 Ser Ile Phe Thr Thr Glu Tyr Gln Gly Gly His His His His His His 385 390 395 400 <210> 62 <211> 364 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly3Ser)3- extracellular domain of mutIL-2 R <400> 62 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 130 135 140 Ser Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro His Ala Thr Phe 145 150 155 160 Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn Cys Glu Cys Lys 165 170 175 Arg Gly Phe Thr Ser Ile Lys Ser Gly Ser Leu Tyr Met Leu Cys Thr 180 185 190 Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys Gln Cys Thr Ser 195 200 205 Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro Gln Pro Glu Glu 210 215 220 Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro Met Gln Pro Val 225 230 235 240 Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro Pro Pro Trp Glu 245 250 255 Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val Gly Gln Met Val 260 265 270 Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His Arg Gly Pro Ala 275 280 285 Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg Trp Thr Gln Pro 290 295 300 Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln Phe Pro Gly Glu 305 310 315 320 Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu Ser Glu Thr Ser 325 330 335 Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr Glu Met Ala Ala 340 345 350 Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 355 360 <210> 63 <211> 1182 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly3Ser)3- extracellular domain of mutIL-2 R Mut <400> 63 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggttc tggtggaggt 480 tcaggtggag gttcggagct ctgtgacgat gacccgccag agatcccaca cgccacattc 540 aaagccatgg cctacaagga aggaaccatg ttgaactgtg aatgcaagag aggtttcacc 600 tcaataaaaa gcgggtcact ctatatgctc tgtacaggaa actctagcca ctcgtcctgg 660 gacaaccaat gtcaatgcac aagctctgcc actcggaaca caacgaaaca agtgacacct 720 caacctgaag aacagaaaga aaggaaaacc acagaaatgc aaagtccaat gcagccagtg 780 gaccaagcga gccttccagg tcactgcagg gaacctccac catgggaaaa tgaagccaca 840 gagagaattt atcatttcgt ggtggggcag atggtttatt atcagtgcgt ccagggatac 900 agggctctac acagaggtcc tgctgagagc gtctgcaaaa tgacccacgg gaagacaagg 960 tggacccagc cccagctcat atgcacaggt gaaatggaga ccagtcagtt tccaggtgaa 1020 gagaagcctc aggcaagccc cgaaggccgt cctgagagtg agacttcctg cctcgtcaca 1080 acaacagatt ttcaaataca gacagaaatg gctgcaacca tggagacgtc catatttaca 1140 acagagtacc agggtggaca tcaccatcac catcactaat aa 1182 <210> 64 <211> 372 <212> PRT <213> Artificial Sequence <220> <223> Synthesized mature form IL-2 (Gly4Ser)4- extracellular domain of mutIL-2 R <400> 64 Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 1 5 10 15 Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 20 25 30 Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 35 40 45 Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 50 55 60 Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 65 70 75 80 Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 85 90 95 Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 100 105 110 Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 115 120 125 Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 130 135 140 Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Leu Cys Asp Asp Asp Pro 145 150 155 160 Pro Glu Ile Pro His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly 165 170 175 Thr Met Leu Asn Cys Glu Cys Lys Arg Gly Phe Thr Ser Ile Lys Ser 180 185 190 Gly Ser Leu Tyr Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp 195 200 205 Asp Asn Gln Cys Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys 210 215 220 Gln Val Thr Pro Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu 225 230 235 240 Met Gln Ser Pro Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His 245 250 255 Cys Arg Glu Pro Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr 260 265 270 His Phe Val Val Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr 275 280 285 Arg Ala Leu His Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His 290 295 300 Gly Lys Thr Arg Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met 305 310 315 320 Glu Thr Ser Gln Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu 325 330 335 Gly Arg Pro Glu Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe 340 345 350 Gln Ile Gln Thr Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr 355 360 365 Thr Glu Tyr Gln 370 <210> 65 <211> 1206 <212> DNA <213> Artificial Sequence <220> <223> Synthesized unprocessed form IL-2 (Gly4Ser)4- extracellular domain of mutIL-2 R <400> 65 atggacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt 60 gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat 120 ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc 180 acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa 240 gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta 300 agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa 360 acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga 420 tggattacct tttgtcaaag catcatctca acactgactg gtggaggtgg atctggtgga 480 ggtggatcag gtggaggtgg atccggtgga ggtggatctg agctctgtga cgatgacccg 540 ccagagatcc cacacgccac attcaaagcc atggcctaca aggaaggaac catgttgaac 600 tgtgaatgca agagaggttt cacctcaata aaaagcgggt cactctatat gctctgtaca 660 ggaaactcta gccactcgtc ctgggacaac caatgtcaat gcacaagctc tgccactcgg 720 aacacaacga aacaagtgac acctcaacct gaagaacaga aagaaaggaa aaccacagaa 780 atgcaaagtc caatgcagcc agtggaccaa gcgagccttc caggtcactg cagggaacct 840 ccaccatggg aaaatgaagc cacagagaga atttatcatt tcgtggtggg gcagatggtt 900 tattatcagt gcgtccaggg atacagggct ctacacagag gtcctgctga gagcgtctgc 960 aaaatgaccc acgggaagac aaggtggacc cagccccagc tcatatgcac aggtgaaatg 1020 gagaccagtc agtttccagg tgaagagaag cctcaggcaa gccccgaagg ccgtcctgag 1080 agtgagactt cctgcctcgt cacaacaaca gattttcaaa tacagacaga aatggctgca 1140 accatggaga cgtccatatt tacaacagag taccagggtg gacatcacca tcaccatcac 1200 taataa 1206