LISTADO DE SECUENCIAS <110> Carlsberg A/S <120> SUSTITUCIONES NUCLEOTÍDICAS PREDETERMINADAS <130> P4150AR00 <160> 26 <170> PatentIn version 3.5 <210> 1 <211> 1089 <212> ADN <213> Hordeum vulgare <400> 1 atgtctcggc tcgccgacct tctcagcctc gacctgtccg gctgcaccgg caagatcatc 60 gccgagtaca tatgggtcgg cggcaccggg atggacgtca ggagcaaagc caggacgctt 120 cccggacccg tggacgaccc cagcaagctt ccaaagtgga atttcgacgg ctccagcacc 180 ggccaagcca cgggcgacga cagcgaagtc atcctccgac cccaagccat cttcagggac 240 ccgttcagga aagggaacaa catcctggtc atctgtgact gctatgcgcc taccggagag 300 ccgattccga gcaacaagcg gtacaacgcg gcgaggatat tcggccatcc tgatgtcaag 360 tctgaagaac catggtatgg gattgagcag gagtacaccc ttctccagaa ggacaccaac 420 tggcccattg gctggccact agggggttac cctggccctc aggggcctta ctactgcgcc 480 gcgggtgcgg agaaatctta cgggcgcgac atcgtcgacg cccactacaa ggcctgcctc 540 tacgccggca tcaacatcgg cggcatcaat gcagaagtca tgccagggca gtgggagttc 600 caagtcggcc cttccgtcgg yatctccgcc ggcgacgagc tctgggcggc tcgctacatt 660 ctcgagagga tcactgagat cgccggcgtc gtcgtctcct tcgaccccaa accgatcccg 720 ggagagtgga acggtgccgg tgcccacaca aactacagca ccaagtcgat gaggagcgag 780 ggcgggtacg aggtgatcaa gagggcgatc aagaagctcg aggcgcggca cacggagcac 840 atagccgcct acggggaagg caacgagcgc cggctcaccg gccgccacga gaccgccgac 900 atcaacacct tcgtatgggg cgtggcaaac cgcggcgcgt cggtgcgggt ggggcgcgac 960 accgagaagg aaggcagggg ctacttcgag gaccggaggc cggcgtccaa catggatccc 1020 tacgtcgtca cctccatgat cgccgagacc accatcctct ggaaggccgg tctctccaat 1080 ggcaagtag 1089 <210> 2 <211> 362 <212> PRT <213> Hordeum vulgare <400> 2 Met Ser Arg Leu Ala Asp Leu Leu Ser Leu Asp Leu Ser Gly Cys Thr 1 5 10 15 Gly Lys Ile Ile Ala Glu Tyr Ile Trp Val Gly Gly Thr Gly Met Asp 20 25 30 Val Arg Ser Lys Ala Arg Thr Leu Pro Gly Pro Val Asp Asp Pro Ser 35 40 45 Lys Leu Pro Lys Trp Asn Phe Asp Gly Ser Ser Thr Gly Gln Ala Thr 50 55 60 Gly Asp Asp Ser Glu Val Ile Leu Arg Pro Gln Ala Ile Phe Arg Asp 65 70 75 80 Pro Phe Arg Lys Gly Asn Asn Ile Leu Val Ile Cys Asp Cys Tyr Ala 85 90 95 Pro Thr Gly Glu Pro Ile Pro Ser Asn Lys Arg Tyr Asn Ala Ala Arg 100 105 110 Ile Phe Gly His Pro Asp Val Lys Ser Glu Glu Pro Trp Tyr Gly Ile 115 120 125 Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Thr Asn Trp Pro Ile Gly 130 135 140 Trp Pro Leu Gly Gly Tyr Pro Gly Pro Gln Gly Pro Tyr Tyr Cys Ala 145 150 155 160 Ala Gly Ala Glu Lys Ser Tyr Gly Arg Asp Ile Val Asp Ala His Tyr 165 170 175 Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Gly Gly Ile Asn Ala Glu 180 185 190 Val Met Pro Gly Gln Trp Glu Phe Gln Val Gly Pro Ser Val Gly Ile 195 200 205 Ser Ala Gly Asp Glu Leu Trp Ala Ala Arg Tyr Ile Leu Glu Arg Ile 210 215 220 Thr Glu Ile Ala Gly Val Val Val Ser Phe Asp Pro Lys Pro Ile Pro 225 230 235 240 Gly Glu Trp Asn Gly Ala Gly Ala His Thr Asn Tyr Ser Thr Lys Ser 245 250 255 Met Arg Ser Glu Gly Gly Tyr Glu Val Ile Lys Arg Ala Ile Lys Lys 260 265 270 Leu Glu Ala Arg His Thr Glu His Ile Ala Ala Tyr Gly Glu Gly Asn 275 280 285 Glu Arg Arg Leu Thr Gly Arg His Glu Thr Ala Asp Ile Asn Thr Phe 290 295 300 Val Trp Gly Val Ala Asn Arg Gly Ala Ser Val Arg Val Gly Arg Asp 305 310 315 320 Thr Glu Lys Glu Gly Arg Gly Tyr Phe Glu Asp Arg Arg Pro Ala Ser 325 330 335 Asn Met Asp Pro Tyr Val Val Thr Ser Met Ile Ala Glu Thr Thr Ile 340 345 350 Leu Trp Lys Ala Gly Leu Ser Asn Gly Lys 355 360 <210> 3 <211> 20 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 3 gtgatcaaga gggcgatcaa 20 <210> 4 <211> 20 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 4 caagtctcaa ctcgccgtat 20 <210> 5 <211> 14 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 5 aagacaacga gcgc 14 <210> 6 <211> 14 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 6 aaggcaacga gcgc 14 <210> 7 <211> 503 <212> PRT <213> Saccharomyces cerevisiae <400> 7 Met Arg Lys Leu Asn Pro Ala Leu Glu Phe Arg Asp Phe Ile Gln Val 1 5 10 15 Leu Lys Asp Glu Asp Asp Leu Ile Glu Ile Thr Glu Glu Ile Asp Pro 20 25 30 Asn Leu Glu Val Gly Ala Ile Met Arg Lys Ala Tyr Glu Ser His Leu 35 40 45 Pro Ala Pro Leu Phe Lys Asn Leu Lys Gly Ala Ser Lys Asp Leu Phe 50 55 60 Ser Ile Leu Gly Cys Pro Ala Gly Leu Arg Ser Lys Glu Lys Gly Asp 65 70 75 80 His Gly Arg Ile Ala His His Leu Gly Leu Asp Pro Lys Thr Thr Ile 85 90 95 Lys Glu Ile Ile Asp Tyr Leu Leu Glu Cys Lys Glu Lys Glu Pro Leu 100 105 110 Pro Pro Ile Thr Val Pro Val Ser Ser Ala Pro Cys Lys Thr His Ile 115 120 125 Leu Ser Glu Glu Lys Ile His Leu Gln Ser Leu Pro Thr Pro Tyr Leu 130 135 140 His Val Ser Asp Gly Gly Lys Tyr Leu Gln Thr Tyr Gly Met Trp Ile 145 150 155 160 Leu Gln Thr Pro Asp Lys Lys Trp Thr Asn Trp Ser Ile Ala Arg Gly 165 170 175 Met Val Val Asp Asp Lys His Ile Thr Gly Leu Val Ile Lys Pro Gln 180 185 190 His Ile Arg Gln Ile Ala Asp Ser Trp Ala Ala Ile Gly Lys Ala Asn 195 200 205 Glu Ile Pro Phe Ala Leu Cys Phe Gly Val Pro Pro Ala Ala Ile Leu 210 215 220 Val Ser Ser Met Pro Ile Pro Glu Gly Val Ser Glu Ser Asp Tyr Val 225 230 235 240 Gly Ala Ile Leu Gly Glu Ser Val Pro Val Val Lys Cys Glu Thr Asn 245 250 255 Asp Leu Met Val Pro Ala Thr Ser Glu Met Val Phe Glu Gly Thr Leu 260 265 270 Ser Leu Thr Asp Thr His Leu Glu Gly Pro Phe Gly Glu Met His Gly 275 280 285 Tyr Val Phe Lys Ser Gln Gly His Pro Cys Pro Leu Tyr Thr Val Lys 290 295 300 Ala Met Ser Tyr Arg Asp Asn Ala Ile Leu Pro Val Ser Asn Pro Gly 305 310 315 320 Leu Cys Thr Asp Glu Thr His Thr Leu Ile Gly Ser Leu Val Ala Thr 325 330 335 Glu Ala Lys Glu Leu Ala Ile Glu Ser Gly Leu Pro Ile Leu Asp Ala 340 345 350 Phe Met Pro Tyr Glu Ala Gln Ala Leu Trp Leu Ile Leu Lys Val Asp 355 360 365 Leu Lys Gly Leu Gln Ala Leu Lys Thr Thr Pro Glu Glu Phe Cys Lys 370 375 380 Lys Val Gly Asp Ile Tyr Phe Arg Thr Lys Val Gly Phe Ile Val His 385 390 395 400 Glu Ile Ile Leu Val Ala Asp Asp Ile Asp Ile Phe Asn Phe Lys Glu 405 410 415 Val Ile Trp Ala Tyr Val Thr Arg His Thr Pro Val Ala Asp Gln Met 420 425 430 Ala Phe Asp Asp Val Thr Ser Phe Pro Leu Ala Pro Phe Val Ser Gln 435 440 445 Ser Ser Arg Ser Lys Thr Met Lys Gly Gly Lys Cys Val Thr Asn Cys 450 455 460 Ile Phe Arg Gln Gln Tyr Glu Arg Ser Phe Asp Tyr Ile Thr Cys Asn 465 470 475 480 Phe Glu Lys Gly Tyr Pro Lys Gly Leu Val Asp Lys Val Asn Glu Asn 485 490 495 Trp Lys Arg Tyr Gly Tyr Lys 500 <210> 8 <211> 101 <212> PRT <213> Triticum aestivum <400> 8 Met Ala Lys Ile Ser Phe Leu Leu Val Ala Leu Leu Val Leu Ala Val 1 5 10 15 Gly Phe Pro Val Glu Val Met Gly Gly Gly Gly Gly Gly Gly Gly Gly 20 25 30 Gly Gly Gly Gly Asn Leu Lys Pro Trp Glu Cys Ser Ser Lys Cys Ser 35 40 45 Ser Arg Cys Ser Gly Thr Gln Tyr Lys Lys Ala Cys Leu Thr Tyr Cys 50 55 60 Asn Lys Cys Cys Ala Thr Cys Leu Cys Val Pro Pro Gly Thr Tyr Gly 65 70 75 80 Asn Lys Gly Ala Cys Pro Cys Tyr Asn Asn Trp Lys Thr Lys Glu Gly 85 90 95 Gly Pro Lys Cys Pro 100 <210> 9 <211> 15 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 9 cgcctgcccc tgcta 15 <210> 10 <211> 31 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 10 agaagaagaa gaagaagaag aaaaccaaga a 31 <210> 11 <211> 17 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 11 caacaactga aagacca 17 <210> 12 <211> 17 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 12 caacaactgg aagacca 17 <210> 13 <211> 17 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 13 catgtttcag acggtgg 17 <210> 14 <211> 20 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 14 catacctcta gcaattgacc 20 <210> 15 <211> 20 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 15 acgtacggaa tgtagattct 20 <210> 16 <211> 18 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 16 acgtacggaa tgtggatt 18 <210> 17 <211> 1260 <212> ADN <213> Hordeum vulgare <400> 17 atggcgtcgt cgagcttcaa ggtgacgcgg atctcggagg gcgcggtgaa gccggcgtcg 60 gagacgcccg accacacgct gccgctggcg tgggtggacc ggtacccgac ccaccgcggc 120 ctggtggagt cgatgcacat cttccggtcc ggcgccgacg cggcccccgg cgtgatccgc 180 gaggcgctgg gcaaggcgct ggccttcttc tacccgctgg cggggcgcat cgtggagcag 240 ccggagaagg ggtgccccgc catccgctgc accgccgacg gcgtctactt cgcggaggcc 300 gtcgccgagt gcagcctgga ggacgtccgg ttcctggagc gccccctgct gctccccaag 360 gaggacctcg tcccctaccc cgccgccgat ctctgggccg tcgagcccca caacaccatc 420 atgatgatgc agatcacgaa attcacatgc ggcgggttcg tgatgggcct ccggttcaac 480 cacgcgtcgg cggacggcat gggcgcggcg cagttcatca aggcggtcgg cgacatggcc 540 cgggggctcc cggagccgac ggtgaagccg gtgtgggaca gggagaagtt ccccaacccg 600 agcatcaagc cgggccctct cccggagctc ccggtgctgg cgctggacta catcgtgctc 660 gacttcccca cgggctacat cgacgggctc aagacgcagt acaaggcgca cagcggcaag 720 ttctgctccg gcttcgacgt gctgacggcc aagctgtggc agtgccgtac ccgggcgctg 780 aacctggagc cggacgccac ggtgaagctg tgcttcttcg ccagcgtgcg ccacctgctg 840 aagctggacg ccgggtacta cggcaactcc atcttccccg tgaagatgtc cgggacgagc 900 aagaaggtgc tggagtcgtc ggtgatggag gtgatcgaca tgatccggga ggccaagcag 960 cggatggcgg tggagttctt ccagttcgcc aaggaggaga cgcggcagga ccccttccag 1020 atgaccttcg actacgagtc catctacgtc tccgactgga gcaagctggg gttctccgac 1080 gtggactacg gcttcggccc gcccatgttc gccggaccgc tcgtcaacaa cgacttcatc 1140 gcctccgtcg tcatcctcaa ggcgccgctg ccgctggacg gcaccaggat gctcgccagc 1200 tgcgtcacca aggagcactc gcaggagttc gcccgcggca tgaaggagga cctgccatga 1260 <210> 18 <211> 1260 <212> ADN <213> Secuencia artificial <220> <223> ADNc mutante <400> 18 atggcgtcgt cgagcttcaa ggtgacgcgg atctcggagg gcgcggtgaa gccggcgtcg 60 gagacgcccg accacacgct gccgctggcg tgagtggacc ggtacccgac ccaccgcggc 120 ctggtggagt cgatgcacat cttccggtcc ggcgccgacg cggcccccgg cgtgatccgc 180 gaggcgctgg gcaaggcgct ggccttcttc tacccgctgg cggggcgcat cgtggagcag 240 ccggagaagg ggtgccccgc catccgctgc accgccgacg gcgtctactt cgcggaggcc 300 gtcgccgagt gcagcctgga ggacgtccgg ttcctggagc gccccctgct gctccccaag 360 gaggacctcg tcccctaccc cgccgccgat ctctgggccg tcgagcccca caacaccatc 420 atgatgatgc agatcacgaa attcacatgc ggcgggttcg tgatgggcct ccggttcaac 480 cacgcgtcgg cggacggcat gggcgcggcg cagttcatca aggcggtcgg cgacatggcc 540 cgggggctcc cggagccgac ggtgaagccg gtgtgggaca gggagaagtt ccccaacccg 600 agcatcaagc cgggccctct cccggagctc ccggtgctgg cgctggacta catcgtgctc 660 gacttcccca cgggctacat cgacgggctc aagacgcagt acaaggcgca cagcggcaag 720 ttctgctccg gcttcgacgt gctgacggcc aagctgtggc agtgccgtac ccgggcgctg 780 aacctggagc cggacgccac ggtgaagctg tgcttcttcg ccagcgtgcg ccacctgctg 840 aagctggacg ccgggtacta cggcaactcc atcttccccg tgaagatgtc cgggacgagc 900 aagaaggtgc tggagtcgtc ggtgatggag gtgatcgaca tgatccggga ggccaagcag 960 cggatggcgg tggagttctt ccagttcgcc aaggaggaga cgcggcagga ccccttccag 1020 atgaccttcg actacgagtc catctacgtc tccgactgga gcaagctggg gttctccgac 1080 gtggactacg gcttcggccc gcccatgttc gccggaccgc tcgtcaacaa cgacttcatc 1140 gcctccgtcg tcatcctcaa ggcgccgctg ccgctggacg gcaccaggat gctcgccagc 1200 tgcgtcacca aggagcactc gcaggagttc gcccgcggca tgaaggagga cctgccatga 1260 <210> 19 <211> 419 <212> PRT <213> Hordeum vulgare <400> 19 Met Ala Ser Ser Ser Phe Lys Val Thr Arg Ile Ser Glu Gly Ala Val 1 5 10 15 Lys Pro Ala Ser Glu Thr Pro Asp His Thr Leu Pro Leu Ala Trp Val 20 25 30 Asp Arg Tyr Pro Thr His Arg Gly Leu Val Glu Ser Met His Ile Phe 35 40 45 Arg Ser Gly Ala Asp Ala Ala Pro Gly Val Ile Arg Glu Ala Leu Gly 50 55 60 Lys Ala Leu Ala Phe Phe Tyr Pro Leu Ala Gly Arg Ile Val Glu Gln 65 70 75 80 Pro Glu Lys Gly Cys Pro Ala Ile Arg Cys Thr Ala Asp Gly Val Tyr 85 90 95 Phe Ala Glu Ala Val Ala Glu Cys Ser Leu Glu Asp Val Arg Phe Leu 100 105 110 Glu Arg Pro Leu Leu Leu Pro Lys Glu Asp Leu Val Pro Tyr Pro Ala 115 120 125 Ala Asp Leu Trp Ala Val Glu Pro His Asn Thr Ile Met Met Met Gln 130 135 140 Ile Thr Lys Phe Thr Cys Gly Gly Phe Val Met Gly Leu Arg Phe Asn 145 150 155 160 His Ala Ser Ala Asp Gly Met Gly Ala Ala Gln Phe Ile Lys Ala Val 165 170 175 Gly Asp Met Ala Arg Gly Leu Pro Glu Pro Thr Val Lys Pro Val Trp 180 185 190 Asp Arg Glu Lys Phe Pro Asn Pro Ser Ile Lys Pro Gly Pro Leu Pro 195 200 205 Glu Leu Pro Val Leu Ala Leu Asp Tyr Ile Val Leu Asp Phe Pro Thr 210 215 220 Gly Tyr Ile Asp Gly Leu Lys Thr Gln Tyr Lys Ala His Ser Gly Lys 225 230 235 240 Phe Cys Ser Gly Phe Asp Val Leu Thr Ala Lys Leu Trp Gln Cys Arg 245 250 255 Thr Arg Ala Leu Asn Leu Glu Pro Asp Ala Thr Val Lys Leu Cys Phe 260 265 270 Phe Ala Ser Val Arg His Leu Leu Lys Leu Asp Ala Gly Tyr Tyr Gly 275 280 285 Asn Ser Ile Phe Pro Val Lys Met Ser Gly Thr Ser Lys Lys Val Leu 290 295 300 Glu Ser Ser Val Met Glu Val Ile Asp Met Ile Arg Glu Ala Lys Gln 305 310 315 320 Arg Met Ala Val Glu Phe Phe Gln Phe Ala Lys Glu Glu Thr Arg Gln 325 330 335 Asp Pro Phe Gln Met Thr Phe Asp Tyr Glu Ser Ile Tyr Val Ser Asp 340 345 350 Trp Ser Lys Leu Gly Phe Ser Asp Val Asp Tyr Gly Phe Gly Pro Pro 355 360 365 Met Phe Ala Gly Pro Leu Val Asn Asn Asp Phe Ile Ala Ser Val Val 370 375 380 Ile Leu Lys Ala Pro Leu Pro Leu Asp Gly Thr Arg Met Leu Ala Ser 385 390 395 400 Cys Val Thr Lys Glu His Ser Gln Glu Phe Ala Arg Gly Met Lys Glu 405 410 415 Asp Leu Pro <210> 20 <211> 13 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 20 cccgaccaca cgc 13 <210> 21 <211> 14 <212> ADN <213> Secuencia artificial <220> <223> Cebador <400> 21 actccaccag gccg 14 <210> 22 <211> 15 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 22 ctggcgtgag tggac 15 <210> 23 <211> 14 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 23 ctggcgtggg tgga 14 <210> 24 <211> 14 <212> ADN <213> Secuencia artificial <220> <223> Sonda <400> 24 ctggcgtggg tgga 14 <210> 25 <211> 306 <212> ADN <213> Triticum aestivum <400> 25 atggccaaga tctccttcct cctcgtggcg ctcctcgtcc tcgccgtcgg gttccccgtg 60 gaggtgatgg gaggtggggg cggcggcggc ggtggcggtg gcggcggcaa cctcaagcca 120 tgggagtgct cgtccaagtg ctcgtcgcgg tgctcgggga cgcagtacaa gaaggcgtgc 180 ctgacctact gcaacaagtg ctgcgccacc tgcctctgcg tgccgccggg cacctacggc 240 aacaagggcg cctgcccctg ctacaacaac tggaagacca aggagggagg ccccaagtgc 300 ccctag 306 <210> 26 <211> 1512 <212> ADN <213> Saccharomyces cerevisiae <400> 26 atgaggaagc taaatccagc tttagaattt agagacttta tccaggtctt aaaagatgaa 60 gatgacttaa tcgaaattac cgaagagatt gatccaaatc tcgaagtagg tgcaattatg 120 aggaaggcct atgaatccca cttaccagcc ccgttattta aaaatctcaa aggtgcttcg 180 aaggatcttt tcagcatttt aggttgccca gccggtttga gaagtaagga gaaaggagat 240 catggtagaa ttgcccatca tctggggctc gacccaaaaa caactatcaa ggaaatcata 300 gattatttgc tggagtgtaa ggagaaggaa cctctccccc caatcactgt tcctgtgtca 360 tctgcacctt gtaaaacaca tatactttct gaagaaaaaa tacatctaca aagcctgcca 420 acaccatatc tacatgtttc agacggtggc aagtacttac aaacgtacgg aatgtggatt 480 cttcaaactc cagataaaaa atggactaat tggtcaattg ctagaggtat ggttgtagat 540 gacaagcata tcactggtct ggtaattaaa ccacaacata ttagacaaat tgctgactct 600 tgggcagcaa ttggaaaagc aaatgaaatt cctttcgcgt tatgttttgg cgttccccca 660 gcagctattt tagttagttc catgccaatt cctgaaggtg tttctgaatc ggattatgtt 720 ggcgcaatct tgggtgagtc ggttccagta gtaaaatgtg agaccaacga tttaatggtt 780 cctgcaacga gtgagatggt atttgagggt actttgtcct taacagatac acatctggaa 840 ggcccatttg gtgagatgca tggatatgtt ttcaaaagcc aaggtcatcc ttgtccattg 900 tacactgtca aggctatgag ttacagagac aatgctattc tacctgtttc gaaccccggt 960 ctttgtacgg atgagacaca taccttgatt ggttcactag tggctactga ggccaaggag 1020 ctggctattg aatctggctt gccaattctg gatgccttta tgccttatga ggctcaggct 1080 ctttggctta tcttaaaggt ggatttgaaa gggctgcaag cattgaagac aacgcctgaa 1140 gaattttgta agaaggtagg tgatatttac tttaggacaa aagttggttt tatagtccat 1200 gaaataattt tggtggcaga tgatatcgac atatttaact tcaaagaagt catctgggcc 1260 tacgttacaa gacatacacc tgttgcagat cagatggctt ttgatgatgt cacttctttt 1320 cctttggctc cctttgtttc gcagtcatcc agaagtaaga ctatgaaagg tggaaagtgc 1380 gttactaatt gcatatttag acagcaatat gagcgcagtt ttgactacat aacttgtaat 1440 tttgaaaagg gatatccaaa aggattagtt gacaaagtaa atgaaaattg gaaaaggtac 1500 ggatataaat aa 1512