LISTADO DE SECUENCIAS <110> CIBUS US LLC CIBUS Europe B.V. LINCOLN et ¨¢l. <120> GENES DE ACETOHIDROXI¨¤CIDO SINTASA MUTADOS EN EUPHORBIACEAE Y MATERIAL VEGETAL QUE COMPRENDE DICHOS GENES <130> CIBUS-032-PCT <140> PCT/US2016/012717 <141> 2016-01-08 <150> US 62/102,009 <151> 2015-01-10 <150> US 62/102,010 <151> 2015-01-10 <160> 10 <170> PatentIn versi¨®n 3.5 <210> 1 <211> 658 <212> PRT <213> Manihot esculenta <220> <221> misc_feature <223> Secuencia de amino¨¢cidos de una prote¨ªna de AHAS de mandioca (Manihot esculenta) de tipo silvestre <400> 1 Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 1 5 10 15 Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 20 25 30 Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 35 40 45 Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 50 55 60 Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 65 70 75 80 Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 85 90 95 Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 100 105 110 Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 115 120 125 Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 130 135 140 Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 145 150 155 160 Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 165 170 175 Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 180 185 190 Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 195 200 205 His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 210 215 220 Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 225 230 235 240 Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 245 250 255 Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 260 265 270 Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 275 280 285 Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 290 295 300 Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 305 310 315 320 Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 325 330 335 Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 340 345 350 Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly 355 360 365 Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 370 375 380 Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 385 390 395 400 Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 405 410 415 Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 420 425 430 Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 435 440 445 Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 450 455 460 Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 465 470 475 480 Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 485 490 495 Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 500 505 510 Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 515 520 525 Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 530 535 540 Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 545 550 555 560 Gln Trp Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 565 570 575 Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 580 585 590 Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 595 600 605 Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 610 615 620 Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 625 630 635 640 Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 645 650 655 Lys Tyr <210> 2 <211> 1977 <212> ADN <213> Manihot esculenta <220> <221> misc_feature <223> Secuencia de nucle¨®tidos que codifica una prote¨ªna de AHAS de mandioca (Manihot esculenta) de tipo silvestre <400> 2 atggcggcgg cgtctacctc tgcggctacc actatcccta aaccctcttc tcacatttct 60 tcctcctcca gatcttcaat cttcatttcc agattcaccc tcccattgtc tctcaacccc 120 caaaaggcca ttcctcaccg ctctctccac atatcaaact ctgtctctaa acctacaacc 180 cctgccccct catcctccac caccttaacc attcctcaag cgtctcctcc caggttttct 240 cctgatgaag ctcgaaaagg cgccgacatc ctcgttgaag cgctggaacg ccaaggggtc 300 actgatgtat ttgcttatcc aggcggcgca tccatggaga tccatcaagc cctgactcgc 360 tcacctataa ttcgcaatgt cctcccgcgc catgagcaag gtggggtctt tgcggctgag 420 ggatatgctc gcgcttctgg caagcctggc gtctgtatcg caacctcggg acccggcgct 480 acaaatctcg taagtggctt ggcagacgct ctccttgaca gcgtccccat tgtggctatc 540 accggccaag ttcctcgccg catgattggc accgacgcat tccaagaaac tcccattgtt 600 gaggtaactc ggtcaataac taagcacaat tacctggtcc ttgatgttga tgatattcct 660 agaattgtaa gtgaagcttt ctttttggcc acctcgggac gtcctggccc agttctgatt 720 gatgtaccaa aagatataca acaacaatta gctgttccaa attggaatac acctattaaa 780 ttgcctggtt acatgtcgag gttgcctaaa gtgcccaacg aatcacattt ggagcagatt 840 gtgaggctaa tttttgagtc aaagaaaccg gttttatacg tgggaggtgg gtgtttaaat 900 tcaagtgagg agttgagaaa gtttgtcgag ttaactggga tcccagtggc tagtactttg 960 atggggctcg gagcattccc agttggccac gaattgtcat tacaaatgct tggaatgcat 1020 ggaactgttt atgctaacta ctcggtggat aagagtgatt tgttgcttgc gtttggggtg 1080 aggtttgatg acagggtgac aggcaagctt gaggcctttg caagcagagc taagattgtt 1140 cacattgata ttgattccgc tgagattggg aaaaataagc agccccatgt gtctgtttgt 1200 gcagatgtga agtttgcctt gcaagggatg aacaagattt tggagagcag atgtgctaag 1260 agtaagctag attttaaggc ttggagggag gagttgaatg agcagaaaag taaatatcca 1320 ttgaaataca agacatttgg agatgcaatt cctcctcagt acgccataca agttctcgat 1380 gaattaacag atgggaatgc cattataagt actggcgttg gacaacatca gatgtgggct 1440 gctcaatttt acaagtacaa gagaccacgg caatggttga cgtcaggggg attaggggct 1500 atgggttttg gattgcctgc cgccattggg gctgctgttg ctaatcctgg tgcagttgtt 1560 gtagatattg atggtgatgg aagttttatc atgaatgtcc aggagttggc aacaattcgt 1620 gtggagaatc tgccaattaa aataatgctt ttgaataatc agcatttggg aatggtggta 1680 caatgggagg accgattcta caaggctaat agagctcata cttatttggg ggatccatca 1740 aaggagtctg agattttccc caatatgttg aagtttgcag aagcttgtgg aatacctgct 1800 gctcgcgtga caagaaaaga gggtcttaga atggcgattc agaaaatgct agatactcca 1860 gggccatact tgttggatgt gattgtgccc catcaagaac atgtgctgcc catgatccca 1920 agtgggggag cttttaagga tgtgataact gagggtgatg gaagaacgaa gtattga 1977 <210> 3 <211> 658 <212> PRT <213> Manihot esculenta <220> <221> misc_feature <223> AHAS de mandioca (Manihot esculenta) que comprende una mutaci¨®n W574S <400> 3 Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 1 5 10 15 Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 20 25 30 Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 35 40 45 Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 50 55 60 Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 65 70 75 80 Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 85 90 95 Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 100 105 110 Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 115 120 125 Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 130 135 140 Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 145 150 155 160 Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 165 170 175 Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 180 185 190 Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 195 200 205 His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 210 215 220 Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 225 230 235 240 Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 245 250 255 Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 260 265 270 Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 275 280 285 Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 290 295 300 Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 305 310 315 320 Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 325 330 335 Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 340 345 350 Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly 355 360 365 Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 370 375 380 Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 385 390 395 400 Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 405 410 415 Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 420 425 430 Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 435 440 445 Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 450 455 460 Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 465 470 475 480 Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 485 490 495 Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 500 505 510 Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 515 520 525 Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 530 535 540 Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 545 550 555 560 Gln Ser Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 565 570 575 Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 580 585 590 Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 595 600 605 Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 610 615 620 Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 625 630 635 640 Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 645 650 655 Lys Tyr <210> 4 <211> 670 <212> PRT <213> Arabidopsis thaliana <220> <221> misc_feature <223> Secuencia de amino¨¢cidos de AHAS de Arabidopsis I <400> 4 Met Ala Ala Ala Thr Thr Thr Thr Thr Thr Ser Ser Ser Ile Ser Phe 1 5 10 15 Ser Thr Lys Pro Ser Pro Ser Ser Ser Lys Ser Pro Leu Pro Ile Ser 20 25 30 Arg Phe Ser Leu Pro Phe Ser Leu Asn Pro Asn Lys Ser Ser Ser Ser 35 40 45 Ser Arg Arg Arg Gly Ile Lys Ser Ser Ser Pro Ser Ser Ile Ser Ala 50 55 60 Val Leu Asn Thr Thr Thr Asn Val Thr Thr Thr Pro Ser Pro Thr Lys 65 70 75 80 Pro Thr Lys Pro Glu Thr Phe Ile Ser Arg Phe Ala Pro Asp Gln Pro 85 90 95 Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu Arg Gln Gly Val 100 105 110 Glu Thr Val Phe Ala Tyr Pro Gly Gly Ala Ser Met Glu Ile His Gln 115 120 125 Ala Leu Thr Arg Ser Ser Ser Ile Arg Asn Val Leu Pro Arg His Glu 130 135 140 Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg Ser Ser Gly Lys 145 150 155 160 Pro Gly Ile Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val 165 170 175 Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro Leu Val Ala Ile 180 185 190 Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp Ala Phe Gln Glu 195 200 205 Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys His Asn Tyr Leu 210 215 220 Val Met Asp Val Glu Asp Ile Pro Arg Ile Ile Glu Glu Ala Phe Phe 225 230 235 240 Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Val Asp Val Pro Lys 245 250 255 Asp Ile Gln Gln Gln Leu Ala Ile Pro Asn Trp Glu Gln Ala Met Arg 260 265 270 Leu Pro Gly Tyr Met Ser Arg Met Pro Lys Pro Pro Glu Asp Ser His 275 280 285 Leu Glu Gln Ile Val Arg Leu Ile Ser Glu Ser Lys Lys Pro Val Leu 290 295 300 Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Asp Glu Leu Gly Arg Phe 305 310 315 320 Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly 325 330 335 Ser Tyr Pro Cys Asp Asp Glu Leu Ser Leu His Met Leu Gly Met His 340 345 350 Gly Thr Val Tyr Ala Asn Tyr Ala Val Glu His Ser Asp Leu Leu Leu 355 360 365 Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala 370 375 380 Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu 385 390 395 400 Ile Gly Lys Asn Lys Thr Pro His Val Ser Val Cys Gly Asp Val Lys 405 410 415 Leu Ala Leu Gln Gly Met Asn Lys Val Leu Glu Asn Arg Ala Glu Glu 420 425 430 Leu Lys Leu Asp Phe Gly Val Trp Arg Asn Glu Leu Asn Val Gln Lys 435 440 445 Gln Lys Phe Pro Leu Ser Phe Lys Thr Phe Gly Glu Ala Ile Pro Pro 450 455 460 Gln Tyr Ala Ile Lys Val Leu Asp Glu Leu Thr Asp Gly Lys Ala Ile 465 470 475 480 Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala Ala Gln Phe Tyr 485 490 495 Asn Tyr Lys Lys Pro Arg Gln Trp Leu Ser Ser Gly Gly Leu Gly Ala 500 505 510 Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ser Val Ala Asn Pro 515 520 525 Asp Ala Ile Val Val Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn 530 535 540 Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu Pro Val Lys Val 545 550 555 560 Leu Leu Leu Asn Asn Gln His Leu Gly Met Val Met Gln Trp Glu Asp 565 570 575 Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Phe Leu Gly Asp Pro Ala 580 585 590 Gln Glu Asp Glu Ile Phe Pro Asn Met Leu Leu Phe Ala Ala Ala Cys 595 600 605 Gly Ile Pro Ala Ala Arg Val Thr Lys Lys Ala Asp Leu Arg Glu Ala 610 615 620 Ile Gln Thr Met Leu Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile 625 630 635 640 Cys Pro His Gln Glu His Val Leu Pro Met Ile Pro Ser Gly Gly Thr 645 650 655 Phe Asn Asp Val Ile Thr Glu Gly Asp Gly Arg Ile Lys Tyr 660 665 670 <210> 5 <211> 658 <212> PRT <213> Manihot esculenta <220> <221> misc_feature <223> Secuencia de amino¨¢cidos de AHAS de mandioca (Manihot esculenta) que comprende una mutaci¨®n D376A <400> 5 Met Ala Ala Ala Ser Thr Ser Ala Ala Thr Thr Ile Pro Lys Pro Ser 1 5 10 15 Ser His Ile Ser Ser Ser Ser Arg Ser Ser Ile Phe Ile Ser Arg Phe 20 25 30 Thr Leu Pro Leu Ser Leu Asn Pro Gln Lys Ala Ile Pro His Arg Ser 35 40 45 Leu His Ile Ser Asn Ser Val Ser Lys Pro Thr Thr Pro Ala Pro Ser 50 55 60 Ser Ser Thr Thr Leu Thr Ile Pro Gln Ala Ser Pro Pro Arg Phe Ser 65 70 75 80 Pro Asp Glu Ala Arg Lys Gly Ala Asp Ile Leu Val Glu Ala Leu Glu 85 90 95 Arg Gln Gly Val Thr Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met 100 105 110 Glu Ile His Gln Ala Leu Thr Arg Ser Pro Ile Ile Arg Asn Val Leu 115 120 125 Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg 130 135 140 Ala Ser Gly Lys Pro Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala 145 150 155 160 Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro 165 170 175 Ile Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp 180 185 190 Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys 195 200 205 His Asn Tyr Leu Val Leu Asp Val Asp Asp Ile Pro Arg Ile Val Ser 210 215 220 Glu Ala Phe Phe Leu Ala Thr Ser Gly Arg Pro Gly Pro Val Leu Ile 225 230 235 240 Asp Val Pro Lys Asp Ile Gln Gln Gln Leu Ala Val Pro Asn Trp Asn 245 250 255 Thr Pro Ile Lys Leu Pro Gly Tyr Met Ser Arg Leu Pro Lys Val Pro 260 265 270 Asn Glu Ser His Leu Glu Gln Ile Val Arg Leu Ile Phe Glu Ser Lys 275 280 285 Lys Pro Val Leu Tyr Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu 290 295 300 Leu Arg Lys Phe Val Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu 305 310 315 320 Met Gly Leu Gly Ala Phe Pro Val Gly His Glu Leu Ser Leu Gln Met 325 330 335 Leu Gly Met His Gly Thr Val Tyr Ala Asn Tyr Ser Val Asp Lys Ser 340 345 350 Asp Leu Leu Leu Ala Phe Gly Val Arg Phe Asp Ala Arg Val Thr Gly 355 360 365 Lys Leu Glu Ala Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile 370 375 380 Asp Ser Ala Glu Ile Gly Lys Asn Lys Gln Pro His Val Ser Val Cys 385 390 395 400 Ala Asp Val Lys Phe Ala Leu Gln Gly Met Asn Lys Ile Leu Glu Ser 405 410 415 Arg Cys Ala Lys Ser Lys Leu Asp Phe Lys Ala Trp Arg Glu Glu Leu 420 425 430 Asn Glu Gln Lys Ser Lys Tyr Pro Leu Lys Tyr Lys Thr Phe Gly Asp 435 440 445 Ala Ile Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Asp 450 455 460 Gly Asn Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala 465 470 475 480 Ala Gln Phe Tyr Lys Tyr Lys Arg Pro Arg Gln Trp Leu Thr Ser Gly 485 490 495 Gly Leu Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala 500 505 510 Val Ala Asn Pro Gly Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser 515 520 525 Phe Ile Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu 530 535 540 Pro Ile Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val 545 550 555 560 Gln Ser Glu Asp Arg Phe Tyr Lys Ala Asn Arg Ala His Thr Tyr Leu 565 570 575 Gly Asp Pro Ser Lys Glu Ser Glu Ile Phe Pro Asn Met Leu Lys Phe 580 585 590 Ala Glu Ala Cys Gly Ile Pro Ala Ala Arg Val Thr Arg Lys Glu Gly 595 600 605 Leu Arg Met Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu 610 615 620 Leu Asp Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro 625 630 635 640 Ser Gly Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Thr 645 650 655 Lys Tyr <210> 6 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> Sint¨¦tica: secuencia espaciadora de la regi¨®n ARNcr <400> 6 acgaggaggg gcttgggttg tgg 23 <210> 7 <211> 27 <212> ADN <213> Secuencia artificial <220> <223> Sint¨¦tica: ARNsg de AHAS1 <400> 7 atttgggaat ggtggtacaa tgggagg 27 <210> 8 <211> 34 <212> ADN <213> Secuencia artificial <220> <223> Sint¨¦tica: ARNsg de AHAS2 <400> 8 gaacaatcaa catctgggta tggttgttca atgg 34 <210> 9 <211> 113 <212> ADN <213> Secuencia artificial <220> <223> Sint¨¦tica: GRON de AHAS1-2-C <400> 9 tctgccaatt aaaataatgc ttttgaataa tcagcatttg ggaatggtgg tacaatcgga 60 agaccgattc tacaaggcta atagagctca tacttatttg ggggatccat caa 113 <210> 10 <211> 113 <212> ADN <213> Secuencia artificial <220> <223> Sint¨¦tica: GRON de AHAS2-2-C <400> 10 acctgcctgt gaagatcttg ttactgaaca atcaacatct gggtatggtt gttcagtcgg 60 aggacagatt ctatcattcc aacagagcac atacatattt agggaaccca tca 113