LISTADO DE SECUENCIAS <110> Stenmark, Pål <120> TricepTox <130> P41705056PCT00 <160> 14 <170> PatentIn versión 3.5 <210> 1 <211> 437 <212> PRT <213> Secuencia Artificial <220> <223> Hc/TriRecABTox <400> 1 Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn 1 5 10 15 His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser 20 25 30 Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn 35 40 45 Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr 50 55 60 Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro 65 70 75 80 Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn 85 90 95 Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu 100 105 110 Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val 115 120 125 Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp 130 135 140 Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr 145 150 155 160 Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn 165 170 175 Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp 180 185 190 Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu 195 200 205 Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 210 215 220 Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro 225 230 235 240 Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys 245 250 255 Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met 260 265 270 Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe 275 280 285 Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val 290 295 300 Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu 305 310 315 320 Trp Arg Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu 325 330 335 Phe Leu Ala Pro Ile Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln 340 345 350 Ile Lys Glu Tyr Asp Glu Gln Gly Thr Asn Ser Cys Gln Leu Leu Phe 355 360 365 Lys Lys Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His 370 375 380 Arg Phe Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe 385 390 395 400 Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn 405 410 415 Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp 420 425 430 Gly Glu Arg Pro Leu 435 <210> 2 <211> 1314 <212> ADN <213> Secuencia Artificial <220> <223> secuencia de Hc/TriRecABToxe optimizada con codones para la expresión en E. coli <400> 2 aagaacatta tcaacaccag catcctgaac ctgcgctacg agagcaacca cctgatcgac 60 ctgagccgct acgcgagcaa gattaacatc ggtagcaagg tgaactttga cccgattgat 120 aaaaaccaga tccaactgtt caacctggaa agcagcaaga tcgaagtgat tctgaaaaac 180 gcgattgttt ataacagcat gtacgaaaac ttcagcacca gcttttggat ccgtattccg 240 aagtatttta acagcatcag cctgaacaac gaatacacca tcattaactg catggagaac 300 aacagcggtt ggaaagtgag cctgaactac ggcgaaatca tttggaccct gcaggacacc 360 caagagatca agcagcgtgt ggttttcaag tacagccaaa tgatcaacat cagcgattac 420 atcaaccgtt ggattttcgt taccatcacc aacaaccgtc tgaacaacag caagatctac 480 attaacggtc gtctgattga ccagaaaccg atcagcaacc tgggcaacat tcacgcgagc 540 aacaacatca tgttcaagct ggacggttgc cgtgataccc accgttatat ctggattaag 600 tacttcaacc tgtttgataa agagctgaac gaaaaggaga ttaaagacct gtatgataac 660 cagagcaaca gcggtatcct gaaggacttt tggggcgatt atctgcaata cgacaaaccg 720 tactatatgt tcaacgcggg taacaagaac agctacatta aactgaagaa agatagcccg 780 gtgggtgaaa tcctgggtcc gcgtggcagc gttatgacca ccaacatcta tctgaacagc 840 agcctgtacc gtggcgagaa gttcatcatt cgtcgtaaaa gcaacagcca gagcattaac 900 gacgatatcg tgcgtaacga agactacatt tatctggatt tctttaacct gaaccaagag 960 tggcgtgttt acacctacaa gtacttcaag aaagaggaag agaagctgtt cctggcgccg 1020 atcagcgaca gcgatgaatt ctacaacacc atccaaatca aggaatacga cgagcagggt 1080 accaacagct gccaactgct gttcaagaaa gacgaagaga gcaccgatga aatcggtctg 1140 atcggcattc accgtttcta cgagagcggc atcgtgttcg aagagtacaa ggattacttc 1200 tgcatcagca agtggtatct gaaagaggtt aagcgtaaac cgtacaacct gaaactgggc 1260 tgcaactggc aatttattcc ggtggatgat ggctggggtg aacgtccgct gtaa 1314 <210> 3 <211> 1311 <212> PRT <213> Secuencia Artificial <220> <223> TriRecABTox inactivo de longitud completa con sitio de activación modificado genéticamente <400> 3 Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 1 5 10 15 Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 20 25 30 Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 35 40 45 Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 50 55 60 Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 65 70 75 80 Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 85 90 95 Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 100 105 110 Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 115 120 125 Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 130 135 140 Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 145 150 155 160 Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 165 170 175 Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 180 185 190 Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 195 200 205 Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Gln 210 215 220 Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 225 230 235 240 Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 245 250 255 Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 260 265 270 Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 275 280 285 Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 290 295 300 Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 305 310 315 320 Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 325 330 335 Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 340 345 350 Asn Phe Val Lys Phe Phe Lys Val Leu Asn Ala Lys Thr Phe Leu Asn 355 360 365 Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 370 375 380 Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 385 390 395 400 Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 405 410 415 Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 420 425 430 Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asp Lys 435 440 445 Gly Tyr Asn Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp 450 455 460 Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn 465 470 475 480 Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu 485 490 495 Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe 500 505 510 Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile 515 520 525 Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly 530 535 540 Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala 545 550 555 560 Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val 565 570 575 Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser 580 585 590 Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu 595 600 605 Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu 610 615 620 Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr 625 630 635 640 Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe 645 650 655 Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile 660 665 670 Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr 675 680 685 Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser 690 695 700 Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn 705 710 715 720 Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met 725 730 735 Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn 740 745 750 Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe 755 760 765 Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala 770 775 780 Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu 785 790 795 800 Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp 805 810 815 Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly 820 825 830 Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr 835 840 845 Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln 850 855 860 Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr 865 870 875 880 Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser 885 890 895 Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro 900 905 910 Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile 915 920 925 Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn 930 935 940 Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile 945 950 955 960 Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser 965 970 975 Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln 980 985 990 Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met 995 1000 1005 Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 1010 1015 1020 Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg 1025 1030 1035 Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala 1040 1045 1050 Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His 1055 1060 1065 Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 1070 1075 1080 Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 1085 1090 1095 Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys 1100 1105 1110 Pro Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys 1115 1120 1125 Leu Lys Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly 1130 1135 1140 Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg 1145 1150 1155 Gly Glu Lys Phe Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile 1160 1165 1170 Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe 1175 1180 1185 Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr Tyr Lys Tyr Phe 1190 1195 1200 Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile Ser Asp Ser 1205 1210 1215 Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp Glu Gln 1220 1225 1230 Gly Thr Asn Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu Ser 1235 1240 1245 Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser 1250 1255 1260 Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys 1265 1270 1275 Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys Leu 1280 1285 1290 Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp Gly Glu 1295 1300 1305 Arg Pro Leu 1310 <210> 4 <211> 3936 <212> ADN <213> Secuencia Artificial <220> <223> TriRecABTox inactivo de longitud completa con sitio de activación modificado genéticamente, secuencia optimizada con codón para la expresión en E. coli <400> 4 atgccgttcg tgaataagca gttcaactac aaagatccgg ttaatggcgt ggacatcgcg 60 tacatcaaaa tcccgaatgc gggtcagatg cagccggtga aggcgttcaa aatccacaac 120 aaaatttggg ttatcccgga gcgtgacacc tttaccaacc cggaggaagg tgatctgaac 180 ccgccgccgg aagcgaaaca agtgccggtt agctactatg acagcaccta tctgagcacc 240 gacaacgaga aggataacta cctgaagggc gtgaccaaac tgttcgaacg tatctacagc 300 accgatctgg gtcgtatgct gctgaccagc attgttcgtg gcatcccgtt ttggggtggc 360 agcaccatcg acaccgaact gaaagtgatt gataccaact gcattaacgt tatccagccg 420 gatggtagct accgtagcga ggaactgaac ctggtgatca ttggcccgag cgcggacatc 480 attcagtttg agtgcaagag cttcggtcac gaagttctga acctgacccg taacggttac 540 ggcagcaccc aatatatccg tttcagcccg gatttcacct ttggcttcga ggaaagcctg 600 gaagtggaca ccaacccgct gctgggtgcg ggcaagtttg cgaccgaccc ggcggttacc 660 ctggcgcacc agctgatcca tgcgggtcac cgtctgtacg gcattgcgat caacccgaac 720 cgtgtgttca aagttaacac caacgcgtac tatgagatga gcggtctgga agtgagcttt 780 gaggaactgc gtaccttcgg tggccacgac gcgaagttta tcgatagcct gcaggagaac 840 gaattccgtc tgtactacta caacaagttc aaggacatcg cgagcaccct gaacaaggcg 900 aaaagcattg tgggtaccac cgcgagcctg caatacatga agaacgtttt caaggagaag 960 tacctgctga gcgaagatac cagcggcaag tttagcgtgg acaagctgaa attcgataag 1020 ctgtataaaa tgctgaccga gatctacacc gaagataact tcgtgaagtt ctttaaagtt 1080 ctgaacgcga aaacctttct gaacttcgac aaggcggttt ttaaaattaa catcgtgccg 1140 aaggttaact acaccatcta tgatggtttc aacctgcgta acaccaacct ggcggcgaac 1200 tttaacggcc agaacaccga gattaacaac atgaacttta ccaagctgaa aaacttcacc 1260 ggtctgtttg aattctataa actgctgtgc gtgcgtggca tcattaccag caagaccaaa 1320 agcctgatcg aaggtcgtga caagggctac aacaaagcgc tgaacgatct gtgcattaaa 1380 gttaacaact gggacctgtt ctttagcccg agcgaggaca acttcaccaa cgatctgaac 1440 aagggcgagg aaatcaccag cgacaccaac attgaagcgg cggaggaaaa catcagcctg 1500 gatctgattc agcaatatta cctgaccttt aacttcgaca acgagccgga aaacattagc 1560 atcgagaacc tgagcagcga catcattggt cagctggagc tgatgccgaa catcgaacgt 1620 ttcccgaacg gcaagaaata cgaactggat aaatatacca tgttccacta cctgcgtgcg 1680 caagagtttg aacacggcaa gagccgtatt gcgctgacca acagcgtgaa cgaggcgctg 1740 ctgaacccga gccgtgttta taccttcttt agcagcgact acgtgaagaa agttaacaaa 1800 gcgaccgagg cggcgatgtt cctgggttgg gtggaacagc tggtttacga ctttaccgat 1860 gaaaccagcg aggtgagcac caccgacaaa attgcggata tcaccatcat tatcccgtat 1920 atcggtccgg cgctgaacat tggcaacatg ctgtacaagg acgattttgt gggtgcgctg 1980 atcttcagcg gcgcggttat cctgctggag ttcattccgg aaattgcgat cccggtgctg 2040 ggtacctttg cgctggttag ctacatcgcg aacaaggtgc tgaccgttca aaccattgat 2100 aacgcgctga gcaagcgtaa cgagaaatgg gacgaagtgt ataaatacat cgttaccaac 2160 tggctggcga aggttaacac ccagattgac ctgatccgta agaaaatgaa agaggcgctg 2220 gaaaaccaag cggaggcgac caaggcgatt atcaactatc agtacaacca atacaccgag 2280 gaagagaaaa acaacattaa cttcaacatc gacgatctga gcagcaagct gaacgaaagc 2340 atcaacaaag cgatgattaa catcaacaag tttctgaacc agtgcagcgt gagctatctg 2400 atgaacagca tgattccgta cggtgttaag cgtctggagg acttcgatgc gagcctgaag 2460 gacgcgctgc tgaaatatat ctacgataac cgtggtaccc tgattggcca agtggaccgt 2520 ctgaaggata aagttaacaa caccctgagc accgatatcc cgttccagct gagcaaatat 2580 gtggacaacc aacgtctgct gagcaccttt accgagtaca tcaagaacat tatcaacacc 2640 agcattctga acctgcgtta tgaaagcaac cacctgatcg acctgagccg ttacgcgagc 2700 aagattaaca tcggtagcaa agttaacttc gacccgatcg ataaaaacca gattcaactg 2760 tttaacctgg agagcagcaa gattgaagtg atcctgaaaa acgcgatcgt ttacaacagc 2820 atgtatgaga actttagcac cagcttctgg attcgtatcc cgaaatattt caacagcatt 2880 agcctgaaca acgagtacac cattatcaac tgcatggaaa acaacagcgg ttggaaggtg 2940 agcctgaact acggcgagat tatctggacc ctgcaggaca cccaagaaat caagcagcgt 3000 gtggttttca agtacagcca aatgatcaac atcagcgatt acattaaccg ttggatcttt 3060 gttaccatta ccaacaaccg tctgaacaac agcaaaattt acatcaacgg tcgtctgatc 3120 gaccagaagc cgattagcaa cctgggcaac atccacgcga gcaacaacat tatgttcaag 3180 ctggacggtt gccgtgatac ccaccgttat atttggatca agtacttcaa cctgttcgat 3240 aaggagctga acgagaagga aatcaaagac ctgtatgata accagagcaa cagcggtatt 3300 ctgaaagact tctggggcga ttacctgcaa tatgacaagc cgtattacat gtttaacgcg 3360 ggtaacaaga acagctacat caaactgaag aaagatagcc cggtgggtga aattctgggt 3420 ccgcgtggca gcgttatgac caccaacatc tatctgaaca gcagcctgta ccgtggcgaa 3480 aagttcatta tccgtcgtaa aagcaacagc cagagcatca acgacgatat tgtgcgtaac 3540 gaggactata tctacctgga tttctttaac ctgaaccaag aatggcgtgt ttacacctac 3600 aagtacttca agaaagaaga ggaaaagctg tttctggcgc cgattagcga cagcgatgaa 3660 ttctataaca ccattcagat caaagagtac gacgaacagg gtaccaacag ctgccaactg 3720 ctgtttaaga aagacgagga aagcaccgat gagatcggtc tgattggcat ccaccgtttt 3780 tacgaaagcg gcatcgtgtt cgaggaatac aaggattact tctgcatcag caagtggtat 3840 ctgaaagagg ttaagcgtaa accgtacaac ctgaaactgg gctgcaactg gcaatttatt 3900 ccggtggatg atggctgggg tgaacgtccg ctgtaa 3936 <210> 5 <211> 1311 <212> PRT <213> Secuencia Artificial <220> <223> TreRecABTox activo de longitud completa con sitio de activación modificado genéticamente <400> 5 Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 1 5 10 15 Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 20 25 30 Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 35 40 45 Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 50 55 60 Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 65 70 75 80 Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 85 90 95 Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 100 105 110 Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 115 120 125 Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 130 135 140 Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 145 150 155 160 Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 165 170 175 Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 180 185 190 Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 195 200 205 Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 210 215 220 Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 225 230 235 240 Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 245 250 255 Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 260 265 270 Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 275 280 285 Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 290 295 300 Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 305 310 315 320 Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 325 330 335 Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 340 345 350 Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 355 360 365 Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 370 375 380 Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 385 390 395 400 Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 405 410 415 Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 420 425 430 Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Ile Glu Gly Arg Asp Lys 435 440 445 Gly Tyr Asn Lys Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp 450 455 460 Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn 465 470 475 480 Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu 485 490 495 Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe 500 505 510 Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile 515 520 525 Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly 530 535 540 Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala 545 550 555 560 Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val 565 570 575 Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser 580 585 590 Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu 595 600 605 Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu 610 615 620 Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr 625 630 635 640 Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe 645 650 655 Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile 660 665 670 Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr 675 680 685 Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser 690 695 700 Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn 705 710 715 720 Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met 725 730 735 Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn 740 745 750 Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe 755 760 765 Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala 770 775 780 Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu 785 790 795 800 Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp 805 810 815 Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly 820 825 830 Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr 835 840 845 Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln 850 855 860 Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr 865 870 875 880 Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser 885 890 895 Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro 900 905 910 Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile 915 920 925 Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn 930 935 940 Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile 945 950 955 960 Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser 965 970 975 Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln 980 985 990 Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln Met 995 1000 1005 Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 1010 1015 1020 Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg 1025 1030 1035 Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala 1040 1045 1050 Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His 1055 1060 1065 Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu 1070 1075 1080 Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 1085 1090 1095 Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys 1100 1105 1110 Pro Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys 1115 1120 1125 Leu Lys Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly 1130 1135 1140 Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg 1145 1150 1155 Gly Glu Lys Phe Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile 1160 1165 1170 Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe 1175 1180 1185 Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr Tyr Lys Tyr Phe 1190 1195 1200 Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile Ser Asp Ser 1205 1210 1215 Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp Glu Gln 1220 1225 1230 Gly Thr Asn Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu Ser 1235 1240 1245 Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser 1250 1255 1260 Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys 1265 1270 1275 Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys Leu 1280 1285 1290 Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp Gly Glu 1295 1300 1305 Arg Pro Leu 1310 <210> 6 <211> 437 <212> PRT <213> Secuencia Artificial <220> <223> Hc/TAB2.1, lazo optimizado 360 <400> 6 Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn 1 5 10 15 His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser 20 25 30 Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn 35 40 45 Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr 50 55 60 Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro 65 70 75 80 Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn 85 90 95 Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu 100 105 110 Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val 115 120 125 Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp 130 135 140 Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr 145 150 155 160 Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn 165 170 175 Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp 180 185 190 Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu 195 200 205 Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser 210 215 220 Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro 225 230 235 240 Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys 245 250 255 Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met 260 265 270 Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe 275 280 285 Ile Ile Arg Arg Lys Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val 290 295 300 Arg Asn Glu Asp Tyr Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu 305 310 315 320 Trp Arg Val Tyr Thr Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu 325 330 335 Phe Leu Ala Pro Ile Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln 340 345 350 Ile Lys Glu Tyr Asp Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe 355 360 365 Lys Lys Asp Glu Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His 370 375 380 Arg Phe Tyr Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe 385 390 395 400 Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn 405 410 415 Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp 420 425 430 Gly Glu Arg Pro Leu 435 <210> 7 <211> 1314 <212> ADN <213> Secuencia Artificial <220> <223> Hc/TAB2.1, optimizado con codones para la expresión en E. coli <400> 7 aagaacatta tcaacaccag catcctgaac ctgcgctacg agagcaacca cctgatcgac 60 ctgagccgct acgcgagcaa gattaacatc ggtagcaagg tgaactttga cccgattgat 120 aaaaaccaga tccaactgtt caacctggaa agcagcaaga tcgaagtgat tctgaaaaac 180 gcgattgttt ataacagcat gtacgaaaac ttcagcacca gcttttggat ccgtattccg 240 aagtatttta acagcatcag cctgaacaac gaatacacca tcattaactg catggagaac 300 aacagcggtt ggaaagtgag cctgaactac ggcgaaatca tttggaccct gcaggacacc 360 caagagatca agcagcgtgt ggttttcaag tacagccaaa tgatcaacat cagcgattac 420 atcaaccgtt ggattttcgt taccatcacc aacaaccgtc tgaacaacag caagatctac 480 attaacggtc gtctgattga ccagaaaccg atcagcaacc tgggcaacat tcacgcgagc 540 aacaacatca tgttcaagct ggacggttgc cgtgataccc accgttatat ctggattaag 600 tacttcaacc tgtttgataa agagctgaac gaaaaggaga ttaaagacct gtatgataac 660 cagagcaaca gcggtatcct gaaggacttt tggggcgatt atctgcaata cgacaaaccg 720 tactatatgt tcaacgcggg taacaagaac agctacatta aactgaagaa agatagcccg 780 gtgggtgaaa tcctgggtcc gcgtggcagc gttatgacca ccaacatcta tctgaacagc 840 agcctgtacc gtggcgagaa gttcatcatt cgtcgtaaaa gcaacagcca gagcattaac 900 gacgatatcg tgcgtaacga agactacatt tatctggatt tctttaacct gaaccaagag 960 tggcgtgttt acacctacaa gtacttcaag aaagaggaag agaagctgtt cctggcgccg 1020 atcagcgaca gcgatgaatt ctacaacacc atccaaatca aggaatacga cgagcagccg 1080 acctatagct gccaactgct gttcaagaaa gacgaagaga gcaccgatga aatcggtctg 1140 atcggcattc accgtttcta cgagagcggc atcgtgttcg aagagtacaa ggattacttc 1200 tgcatcagca agtggtatct gaaagaggtt aagcgtaaac cgtacaacct gaaactgggc 1260 tgcaactggc aatttattcc ggtggatgat ggctggggtg aacgtccgct gtaa 1314 <210> 8 <211> 432 <212> PRT <213> Secuencia Artificial <220> <223> Hc/TAB2.1.1 <400> 8 Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu 1 5 10 15 Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp 20 25 30 Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys 35 40 45 Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu 50 55 60 Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser 65 70 75 80 Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn 85 90 95 Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu 100 105 110 Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln 115 120 125 Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 130 135 140 Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu 145 150 155 160 Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn 165 170 175 Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile 180 185 190 Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu 195 200 205 Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp 210 215 220 Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Phe Asn 225 230 235 240 Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp Ser Pro Val 245 250 255 Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr 260 265 270 Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe Ile Ile Arg Arg Lys 275 280 285 Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr 290 295 300 Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr 305 310 315 320 Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile 325 330 335 Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp 340 345 350 Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu 355 360 365 Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser 370 375 380 Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys Trp 385 390 395 400 Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu Lys Leu Gly Cys 405 410 415 Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu 420 425 430 <210> 9 <211> 1299 <212> ADN <213> Secuencia Artificial <220> <223> Hc/TAB2.1.1, optimizado con codones para la expresión en E. coli <400> 9 accagcatcc tgaacctgcg ctacgagagc aaccacctga tcgacctgag ccgctacgcg 60 agcaagatta acatcggtag caaggtgaac tttgacccga ttgataaaaa ccagatccaa 120 ctgttcaacc tggaaagcag caagatcgaa gtgattctga aaaacgcgat tgtttataac 180 agcatgtacg aaaacttcag caccagcttt tggatccgta ttccgaagta ttttaacagc 240 atcagcctga acaacgaata caccatcatt aactgcatgg agaacaacag cggttggaaa 300 gtgagcctga actacggcga aatcatttgg accctgcagg acacccaaga gatcaagcag 360 cgtgtggttt tcaagtacag ccaaatgatc aacatcagcg attacatcaa ccgttggatt 420 ttcgttacca tcaccaacaa ccgtctgaac aacagcaaga tctacattaa cggtcgtctg 480 attgaccaga aaccgatcag caacctgggc aacattcacg cgagcaacaa catcatgttc 540 aagctggacg gttgccgtga tacccaccgt tatatctgga ttaagtactt caacctgttt 600 gataaagagc tgaacgaaaa ggagattaaa gacctgtatg ataaccagag caacagcggt 660 atcctgaagg acttttgggg cgattatctg caatacgaca aaccgtacta tatgttcaac 720 gcgggtaaca agaacagcta cattaaactg aagaaagata gcccggtggg tgaaatcctg 780 ggtccgcgtg gcagcgttat gaccaccaac atctatctga acagcagcct gtaccgtggc 840 gagaagttca tcattcgtcg taaaagcaac agccagagca ttaacgacga tatcgtgcgt 900 aacgaagact acatttatct ggatttcttt aacctgaacc aagagtggcg tgtttacacc 960 tacaagtact tcaagaaaga ggaagagaag ctgttcctgg cgccgatcag cgacagcgat 1020 gaattctaca acaccatcca aatcaaggaa tacgacgagc agccgaccta tagctgccaa 1080 ctgctgttca agaaagacga agagagcacc gatgaaatcg gtctgatcgg cattcaccgt 1140 ttctacgaga gcggcatcgt gttcgaagag tacaaggatt acttctgcat cagcaagtgg 1200 tatctgaaag aggttaagcg taaaccgtac aacctgaaac tgggctgcaa ctggcaattt 1260 attccggtgg atgatggctg gggtgaacgt ccgctgtaa 1299 <210> 10 <211> 424 <212> PRT <213> Secuencia Artificial <220> <223> Hc/TAB2.1.3, aumento de solubilidad <400> 10 Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu 1 5 10 15 Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp 20 25 30 Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys 35 40 45 Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu 50 55 60 Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser 65 70 75 80 Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn 85 90 95 Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu 100 105 110 Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser Gln 115 120 125 Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr Ile 130 135 140 Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu 145 150 155 160 Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn 165 170 175 Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr Ile 180 185 190 Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys Glu 195 200 205 Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys Asp 210 215 220 Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Phe Asn 225 230 235 240 Ala Gly Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp Ser Pro Val 245 250 255 Gly Glu Ile Leu Gly Pro Arg Gly Ser Val Met Thr Thr Asn Ile Tyr 260 265 270 Leu Asn Ser Ser Leu Tyr Arg Gly Glu Lys Phe Ile Ile Arg Arg Lys 275 280 285 Ser Asn Ser Gln Ser Ile Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr 290 295 300 Ile Tyr Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr 305 310 315 320 Tyr Lys Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile 325 330 335 Ser Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp 340 345 350 Glu Gln Pro Thr Tyr Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu 355 360 365 Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Asn Asn Lys 370 375 380 Asp Tyr Phe Cys Ile Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys 385 390 395 400 Pro Tyr Asn Leu Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp 405 410 415 Asp Gly Trp Gly Glu Arg Pro Leu 420 <210> 11 <211> 1275 <212> ADN <213> Secuencia Artificial <220> <223> Hc/TAB2.1.3, optimizado con codones para la expresión en E. coli <400> 11 accagcatcc tgaacctgcg ctacgagagc aaccacctga tcgacctgag ccgctacgcg 60 agcaagatta acatcggtag caaggtgaac tttgacccga ttgataaaaa ccagatccaa 120 ctgttcaacc tggaaagcag caagatcgaa gtgattctga aaaacgcgat tgtttataac 180 agcatgtacg aaaacttcag caccagcttt tggatccgta ttccgaagta ttttaacagc 240 atcagcctga acaacgaata caccatcatt aactgcatgg agaacaacag cggttggaaa 300 gtgagcctga actacggcga aatcatttgg accctgcagg acacccaaga gatcaagcag 360 cgtgtggttt tcaagtacag ccaaatgatc aacatcagcg attacatcaa ccgttggatt 420 ttcgttacca tcaccaacaa ccgtctgaac aacagcaaga tctacattaa cggtcgtctg 480 attgaccaga aaccgatcag caacctgggc aacattcacg cgagcaacaa catcatgttc 540 aagctggacg gttgccgtga tacccaccgt tatatctgga ttaagtactt caacctgttt 600 gataaagagc tgaacgaaaa ggagattaaa gacctgtatg ataaccagag caacagcggt 660 atcctgaagg acttttgggg cgattatctg caatacgaca aaccgtacta tatgttcaac 720 gcgggtaaca agaacagcta cattaaactg aagaaagata gcccggtggg tgaaatcctg 780 ggtccgcgtg gcagcgttat gaccaccaac atctatctga acagcagcct gtaccgtggc 840 gagaagttca tcattcgtcg taaaagcaac agccagagca ttaacgacga tatcgtgcgt 900 aacgaagact acatttatct ggatttcttt aacctgaacc aagagtggcg tgtttacacc 960 tacaagtact tcaagaaaga ggaagagaag ctgttcctgg cgccgatcag cgacagcgat 1020 gaattctaca acaccatcca aatcaaggaa tacgacgagc agccgaccta tagctgccaa 1080 ctgctgttca agaaagacga agagagcacc gatgaaatcg gtctgatcgg cattcaccgt 1140 ttcaacaaca aggattactt ctgcatcagc aagtggtatc tgaaagaggt taagcgtaaa 1200 ccgtacaacc tgaaactggg ctgcaactgg caatttattc cggtggatga tggctggggt 1260 gaacgtccgc tgtaa 1275 <210> 12 <211> 1343 <212> PRT <213> Secuencia Artificial <220> <223> BoNT/TAB2.1.3, proteína activa de longitud completa <400> 12 Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 1 5 10 15 Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 20 25 30 Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 35 40 45 Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 50 55 60 Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 65 70 75 80 Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 85 90 95 Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 100 105 110 Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 115 120 125 Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 130 135 140 Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 145 150 155 160 Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 165 170 175 Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 180 185 190 Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 195 200 205 Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Ala 210 215 220 Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 225 230 235 240 Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 245 250 255 Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 260 265 270 Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 275 280 285 Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 290 295 300 Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 305 310 315 320 Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 325 330 335 Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 340 345 350 Asn Phe Val Lys Phe Phe Lys Val Leu Asn Ala Lys Thr Phe Leu Asn 355 360 365 Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 370 375 380 Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 385 390 395 400 Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 405 410 415 Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 420 425 430 Gly Ile Ile Thr Ser Lys Ala Gly Ala Gly Lys Ser Leu Val Pro Arg 435 440 445 Gly Ser Ala Gly Ala Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn 450 455 460 Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp 465 470 475 480 Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala 485 490 495 Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe 500 505 510 Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser 515 520 525 Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro 530 535 540 Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu 545 550 555 560 Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn 565 570 575 Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe 580 585 590 Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met 595 600 605 Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr 610 615 620 Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile 625 630 635 640 Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp 645 650 655 Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu 660 665 670 Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val 675 680 685 Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala 690 695 700 Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val 705 710 715 720 Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys 725 730 735 Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile 740 745 750 Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile 755 760 765 Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn 770 775 780 Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser 785 790 795 800 Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp 805 810 815 Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn 820 825 830 Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn 835 840 845 Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp 850 855 860 Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile 865 870 875 880 Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp 885 890 895 Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe 900 905 910 Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser 915 920 925 Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr 930 935 940 Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn 945 950 955 960 Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn 965 970 975 Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr 980 985 990 Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser 995 1000 1005 Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val 1010 1015 1020 Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn 1025 1030 1035 Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile 1040 1045 1050 His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp 1055 1060 1065 Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys 1070 1075 1080 Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser 1085 1090 1095 Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr 1100 1105 1110 Asp Lys Pro Tyr Tyr Met Phe Asn Ala Gly Asn Lys Asn Ser Tyr 1115 1120 1125 Ile Lys Leu Lys Lys Asp Ser Pro Val Gly Glu Ile Leu Gly Pro 1130 1135 1140 Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu 1145 1150 1155 Tyr Arg Gly Glu Lys Phe Ile Ile Arg Arg Lys Ser Asn Ser Gln 1160 1165 1170 Ser Ile Asn Asp Asp Ile Val Arg Asn Glu Asp Tyr Ile Tyr Leu 1175 1180 1185 Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr Thr Tyr Lys 1190 1195 1200 Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe Leu Ala Pro Ile Ser 1205 1210 1215 Asp Ser Asp Glu Phe Tyr Asn Thr Ile Gln Ile Lys Glu Tyr Asp 1220 1225 1230 Glu Gln Gly Thr Asn Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu 1235 1240 1245 Glu Ser Thr Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr 1250 1255 1260 Glu Ser Gly Ile Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile 1265 1270 1275 Ser Lys Trp Tyr Leu Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu 1280 1285 1290 Lys Leu Gly Cys Asn Trp Gln Phe Ile Pro Val Asp Asp Gly Trp 1295 1300 1305 Gly Glu Arg Pro Leu Val Pro Arg Gly Ser Ala Asn Ser Ser Ser 1310 1315 1320 Val Asp Lys Leu Trp Ser His Pro Gln Phe Glu Lys Leu Glu His 1325 1330 1335 His His His His His 1340 <210> 13 <211> 4032 <212> ADN <213> Secuencia Artificial <220> <223> BoNT/TAB2.1.3, secuencia incluida en el vector pEt29(a) para la expresión en E. coli <400> 13 atgccatttg tgaacaagca gtttaactat aaggacccgg tgaacggtgt ggatatcgcg 60 tatatcaaaa tcccgaatgc gggccagatg caaccagtca aggcgttcaa gattcataac 120 aagatttggg ttattccgga acgtgatacc ttcaccaatc cggaagaagg cgacttaaac 180 ccgccgccag aagccaaaca agtgccggtg agctactatg atagcacgta tcttagcacc 240 gataatgaaa aagacaatta cctgaagggc gtgaccaagt tgttcgagcg catctacagt 300 accgacttag gccgcatgtt gttgacgagc atcgttcgcg gtatcccgtt ctggggcggc 360 tcgaccattg ataccgagtt gaaagtcatt gacacgaact gtatcaatgt tatccaaccg 420 gacggcagtt atcgcagcga ggagttaaat ttggtcatca tcggtccaag cgcagatatt 480 attcagttcg aatgcaagag cttcggccat gaggtcttga atttgacgcg caacggttac 540 ggcagcaccc aatacatccg ctttagcccg gatttcacct ttggcttcga ggagagcttg 600 gaggtggaca ccaacccgct gttaggtgcc ggcaaattcg caaccgaccc ggcagtgacg 660 ttggcgcacg cgttgattca tgcgggtcac cgcttatacg gtatcgcgat caatccgaat 720 cgcgtcttta aagtcaatac caacgcgtac tacgaaatga gcggcttaga ggttagcttt 780 gaagaattac gcaccttcgg tggccacgac gccaagttca tcgacagcct gcaggaaaat 840 gagttccgct tgtactatta caataaattc aaggacatcg cgagcacctt aaataaagca 900 aagagcattg tgggcaccac cgcaagcttg cagtacatga agaacgtatt taaggaaaaa 960 tatttgttgt cggaggatac cagcgggaaa ttcagcgtcg ataagctgaa attcgacaaa 1020 ttgtataaaa tgctgaccga gatttacacc gaggataact tcgtcaagtt ttttaaggtg 1080 ttaaatgcga agaccttttt aaactttgat aaagcggtgt ttaaaattaa tatcgtgccg 1140 aaggtgaatt acaccatcta cgatggtttc aatttacgca acacgaatct ggcggcgaat 1200 tttaatggcc aaaacaccga aattaacaac atgaacttta cgaagttaaa gaatttcacg 1260 ggcttattcg aattctacaa gttattatgc gtgcgcggca tcattaccag caaggcaggt 1320 gcgggcaagt ccttggttcc gcgtggcagc gccggcgccg gcgcgctcaa tgatctgtgt 1380 attaaagtca ataactggga cctgttcttc agcccgagcg aggataactt taccaacgac 1440 ttaaacaaag gcgaggagat cacgagcgat acgaacatcg aggcggcgga ggaaaatatt 1500 agcctggacc tcattcagca gtactatctg acgttcaatt ttgacaatga gccggagaac 1560 atcagcattg aaaatctcag cagcgacatc atcggtcagt tggaactgat gccgaacatt 1620 gaacgctttc cgaacggcaa aaaatatgaa ctggacaagt ataccatgtt ccattactta 1680 cgcgcacagg aatttgagca cggcaagagc cgcattgcgc tgaccaatag cgttaacgag 1740 gccttgttaa atccgagccg tgtctacacg ttcttcagca gcgattatgt caaaaaagtg 1800 aacaaggcga ccgaagccgc gatgtttttg ggctgggtcg agcaattggt ttacgatttt 1860 accgacgaaa ccagcgaggt gagcacgacc gacaaaattg cagatatcac catcatcatt 1920 ccgtacatcg gtccggcgct caatatcggc aatatgttat acaaggacga ctttgtgggc 1980 gcgctgatct ttagcggcgc ggttatctta ttagaattca tcccggagat cgcaatcccg 2040 gtcttgggca cctttgcgtt ggtgagctat atcgcgaata aagtgctcac ggtccaaacc 2100 atcgataacg cgctcagcaa gcgtaatgag aaatgggacg aggtttataa gtatatcgtg 2160 accaactggt tagcaaaagt caatacgcag atcgatctca tccgcaaaaa aatgaaagaa 2220 gccttggaaa atcaagcgga ggcaaccaaa gccatcatta attaccagta taaccaatat 2280 accgaagaag aaaaaaacaa tatcaacttc aatatcgatg atttgagcag caaactgaac 2340 gagagcatta acaaagcgat gattaacatc aacaagttct tgaatcaatg cagcgtgagc 2400 tatctcatga acagcatgat cccgtatggc gtcaaacgct tggaagattt tgacgccagc 2460 ctgaaagatg cgctcctcaa gtatatttat gacaaccgcg gcaccctcat tggccaggtg 2520 gaccgcttga aggataaagt gaacaatacg ctcagcacgg atatcccgtt ccagctgagc 2580 aagtacgtcg acaaccagcg cttactgagc acctttaccg agtatatcaa gaacatcatt 2640 aataccagca tcctcaactt gcgctatgag agcaatcacc tgatcgacct cagccgctac 2700 gccagcaaga tcaacatcgg cagcaaggtc aatttcgacc cgatcgataa gaatcagatc 2760 caattgttta acctggaaag cagcaagatc gaggttatct tgaagaacgc gattgtgtac 2820 aacagcatgt atgagaactt tagcaccagc ttctggattc gtatcccgaa atatttcaac 2880 agcattagcc tgaacaacga gtacaccatt atcaactgca tggaaaacaa cagcggttgg 2940 aaggtgagcc tgaactacgg cgagattatc tggaccctgc aggacaccca agaaatcaag 3000 cagcgtgtgg ttttcaagta cagccaaatg atcaacatca gcgattacat taaccgttgg 3060 atctttgtta ccattaccaa caaccgtctg aacaacagca aaatttacat caacggtcgt 3120 ctgatcgacc agaagccgat tagcaacctg ggcaacatcc acgcgagcaa caacattatg 3180 ttcaagctgg acggttgccg tgatacccac cgttatattt ggatcaagta cttcaacctg 3240 ttcgataagg agctgaacga gaaggaaatc aaagacctgt atgataacca gagcaacagc 3300 ggtattctga aagacttctg gggcgattac ctgcaatatg acaagccgta ttacatgttt 3360 aacgcgggta acaagaacag ctacatcaaa ctgaagaaag atagcccggt gggtgaaatt 3420 ctgggtccgc gtggcagcgt tatgaccacc aacatctatc tgaacagcag cctgtaccgt 3480 ggcgaaaagt tcattatccg tcgtaaaagc aacagccaga gcatcaacga cgatattgtg 3540 cgtaacgagg actatatcta cctggatttc tttaacctga accaagaatg gcgtgtttac 3600 acctacaagt acttcaagaa agaagaggaa aagctgtttc tggcgccgat tagcgacagc 3660 gatgaattct ataacaccat tcagatcaaa gagtacgacg aacagggtac caacagctgc 3720 caactgctgt ttaagaaaga cgaggaaagc accgatgaga tcggtctgat tggcatccac 3780 cgtttttacg aaagcggcat cgtgttcgag gaatacaagg attacttctg catcagcaag 3840 tggtatctga aagaggttaa gcgtaaaccg tacaacctga aactgggctg caactggcaa 3900 tttattccgg tggatgatgg ctggggtgaa cgtccactag tgccacgcgg ttccgcgaat 3960 tcgagctccg tcgacaagct ttggagccac ccgcagttcg aaaaactcga gcaccaccac 4020 caccaccact ga 4032 <210> 14 <211> 21 <212> PRT <213> Secuencia Artificial <220> <223> péptido hSytI <400> 14 Gly Glu Gly Lys Glu Asp Ala Phe Ser Lys Leu Lys Glu Lys Phe Met 1 5 10 15 Asn Glu Leu His Lys 20