LISTADO DE SECUENCIAS <110> ERBER AG <120> MEDIOS Y MÉTODOS PARA EL CLIVAJE DE ZEARALENONA <130> P4757AR00 <160> 11 <170> PatentIn versión 3.5 <210> 1 <211> 309 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 1 Met Val Thr Ser Pro Ala Leu Arg Asp Val His Val Pro His Ala Tyr 1 5 10 15 Pro Glu Gln Gln Val Asp Leu Gly Glu Ile Thr Met Asn Tyr Ala Glu 20 25 30 Ala Gly Asp Pro Asp Arg Pro Ala Val Leu Leu Ile Pro Glu Gln Thr 35 40 45 Gly Ser Trp Trp Ser Tyr Glu Glu Ala Met Gly Leu Leu Ser Glu His 50 55 60 Phe His Val Tyr Ala Val Asp Leu Arg Gly Gln Gly Arg Ser Ser Trp 65 70 75 80 Thr Pro Lys Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg 85 90 95 Phe Ile Ala Leu Val Val Lys Arg Pro Val Val Val Ala Gly Asn Ser 100 105 110 Ser Gly Gly Val Leu Ala Ala Trp Leu Ser Ala Tyr Ser Met Pro Gly 115 120 125 Gln Leu Arg Gly Val Leu Cys Glu Asp Pro Pro Phe Phe Ala Ser Glu 130 135 140 Leu Val Pro Ala His Gly His Ser Val Arg Gln Gly Ala Gly Pro Val 145 150 155 160 Phe Glu Leu Phe Arg Thr Tyr Leu Gly Asp Gln Trp Ser Val Gly Asp 165 170 175 Trp Glu Gly Phe Cys Arg Ala Ala Gly Ala Ser Ala Ser Pro Met Ala 180 185 190 Arg Ser Phe Val Ala Asp Gly Ile Pro Gln His Leu Gln Glu Tyr Asp 195 200 205 Pro Glu Trp Ala Arg Val Phe Tyr Glu Gly Thr Val Gly Leu Ser Cys 210 215 220 Pro His Glu Arg Met Leu Gly Gln Val Lys Thr Pro Val Leu Leu Thr 225 230 235 240 His His Met Arg Gly Ile Asp Pro Glu Thr Gly Asn Leu Leu Gly Ala 245 250 255 Leu Ser Asp Glu Gln Ala Leu Arg Ala Arg Arg Leu Met Asp Ser Ala 260 265 270 Gly Val Thr Val Asp Tyr Glu Ser Val Pro Asp Ala Ser His Met Met 275 280 285 His Gln Ser Ala Pro Ala Arg Tyr Val Glu Ile Phe Thr Arg Trp Ala 290 295 300 Ala Ala Leu Ala Pro 305 <210> 2 <211> 308 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 2 Met Ala Asp Pro Ala Gln Arg Asp Val Tyr Val Pro His Ala Tyr Pro 1 5 10 15 Glu Lys Gln Ala Asp Leu Gly Glu Ile Thr Met Asn Tyr Ala Glu Ala 20 25 30 Gly Glu Pro Asp Met Pro Ala Val Leu Leu Ile Pro Glu Gln Thr Gly 35 40 45 Ser Trp Trp Gly Tyr Glu Glu Ala Met Gly Leu Leu Ala Glu Asn Phe 50 55 60 His Val Tyr Ala Val Asp Leu Arg Gly Gln Gly Arg Ser Ser Trp Ala 65 70 75 80 Pro Lys Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg Phe 85 90 95 Ile Ala Leu Val Val Lys Arg Pro Val Ile Val Ala Gly Asn Ser Ser 100 105 110 Gly Gly Val Leu Ala Ala Trp Leu Ser Ala Tyr Ser Met Pro Gly Gln 115 120 125 Val Arg Gly Ala Leu Cys Glu Asp Ala Pro Phe Phe Ala Ser Glu Leu 130 135 140 Val Thr Thr Cys Gly His Ser Ile Arg Gln Ala Ala Gly Pro Met Phe 145 150 155 160 Glu Leu Phe Arg Thr Tyr Leu Gly Asp Gln Trp Ser Val Gly Asp Trp 165 170 175 Thr Gly Tyr Cys Arg Ala Ala Asp Ala Ser Ser Ser Pro Met Ala Arg 180 185 190 Tyr Phe Val Ala Asp Glu Ile Pro Gln His Met Arg Glu Tyr Asp Pro 195 200 205 Glu Trp Ala Arg Ala Phe Trp Glu Gly Thr Val Ala Leu His Cys Pro 210 215 220 His Glu Gln Leu Leu Thr Gln Val Lys Thr Pro Val Leu Leu Thr His 225 230 235 240 His Met Arg Asp Ile Asp Pro Asp Thr Gly His Leu Val Gly Ala Leu 245 250 255 Ser Asp Glu Gln Ala Ala Arg Ala Arg Leu Leu Met Glu Ser Ala Gly 260 265 270 Val Lys Val Asp Tyr Ala Ser Val Pro Asp Ala Leu His Met Met His 275 280 285 Gln Phe Asp Pro Pro Arg Tyr Val Glu Ile Phe Thr Gln Trp Ala Ala 290 295 300 Thr Leu Ala Ala 305 <210> 3 <211> 309 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 3 Met Val Thr Ser Pro Ala Leu Arg Asp Val His Val Pro His Ala Tyr 1 5 10 15 Pro Glu Gln Gln Val Asp Leu Gly Glu Ile Thr Met Asn Tyr Ala Glu 20 25 30 Ala Gly Asp Pro Gly Arg Pro Ala Val Leu Leu Ile Pro Glu Gln Thr 35 40 45 Gly Ser Trp Trp Ser Tyr Glu Glu Ala Met Gly Leu Leu Ala Glu His 50 55 60 Phe His Val Tyr Ala Val Asp Leu Arg Gly Gln Gly Arg Ser Ser Trp 65 70 75 80 Thr Pro Lys Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg 85 90 95 Phe Ile Ala Leu Val Val Arg Arg Pro Val Val Val Ala Gly Asn Ser 100 105 110 Ser Gly Gly Val Leu Ala Ala Trp Leu Ser Ala Tyr Ser Met Pro Gly 115 120 125 Gln Ile Arg Gly Val Leu Cys Glu Asp Pro Pro Phe Phe Ala Ser Glu 130 135 140 Leu Val Pro Ala His Gly His Ser Val Arg Gln Gly Ala Gly Pro Val 145 150 155 160 Phe Glu Leu Phe Arg Thr Tyr Leu Gly Asp Gln Trp Ser Val Gly Asp 165 170 175 Trp Glu Gly Phe Arg Ser Ala Ala Asp Ala Ser Ala Ser Pro Met Ala 180 185 190 Arg Ser Phe Val Ala Asp Thr Ile Pro Gln His Leu Lys Glu Tyr Asp 195 200 205 Pro Glu Trp Ala Arg Ala Phe Tyr Glu Gly Thr Val Gly Leu Asn Cys 210 215 220 Pro His Glu Arg Met Leu Asn Arg Val Asn Thr Pro Val Leu Leu Thr 225 230 235 240 His His Met Arg Gly Thr Asp Pro Glu Thr Gly Asn Leu Leu Gly Ala 245 250 255 Leu Ser Asp Glu Gln Ala Ala Gln Val Arg Arg Leu Met Glu Ser Ala 260 265 270 Gly Val Lys Val Asp Tyr Glu Ser Val Pro Asp Ala Ser His Met Met 275 280 285 His Gln Ser Asp Pro Ala Arg Tyr Ala Glu Ile Leu Thr Pro Trp Thr 290 295 300 Ala Ala Leu Ala Pro 305 <210> 4 <211> 309 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 4 Met Val Thr Ser Pro Ala Leu Arg Asp Val His Val Pro His Ala Tyr 1 5 10 15 Pro Glu Gln Gln Val Asp Leu Gly Glu Ile Thr Met Asn Tyr Ala Glu 20 25 30 Ala Gly Asp Pro Asp Arg Pro Ala Val Leu Leu Ile Pro Glu Gln Thr 35 40 45 Gly Ser Trp Trp Ser Tyr Glu Glu Ala Met Gly Leu Leu Ala Glu His 50 55 60 Phe His Val Tyr Ala Val Asp Leu Arg Gly Gln Gly Arg Ser Ser Trp 65 70 75 80 Thr Pro Lys Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg 85 90 95 Phe Ile Ala Leu Val Val Lys Arg Pro Val Val Val Ala Gly Asn Ser 100 105 110 Ser Gly Gly Val Leu Ala Ala Trp Leu Ser Ala Tyr Ser Met Pro Gly 115 120 125 Gln Leu Arg Gly Val Leu Cys Glu Asp Pro Pro Phe Phe Ala Ser Glu 130 135 140 Leu Val Pro Ala His Gly His Ser Val Arg Gln Gly Ala Gly Pro Val 145 150 155 160 Phe Glu Leu Phe Arg Thr Tyr Leu Gly Asp Gln Trp Ser Val Ser Asp 165 170 175 Trp Glu Gly Phe Cys Arg Ala Ala Gly Ala Ser Ala Ser Pro Met Ala 180 185 190 Arg Ser Phe Val Ala Asp Gly Ile Pro Gln His Leu Lys Glu Tyr Asp 195 200 205 Pro Glu Trp Ala Arg Ala Phe His Glu Gly Thr Val Gly Leu Asn Cys 210 215 220 Pro His Glu Arg Met Leu Gly Arg Val Asn Thr Pro Val Leu Leu Thr 225 230 235 240 His His Met Arg Gly Thr Asp Pro Glu Thr Gly Asn Leu Leu Gly Ala 245 250 255 Leu Ser Asp Glu Gln Ala Ala Gln Ala Arg Leu Leu Met Glu Ser Ala 260 265 270 Gly Val Arg Val Asp Tyr Glu Ser Val Pro Asp Ala Ser His Met Met 275 280 285 His Gln Ser Asp Pro Ala Arg Tyr Ala Glu Ile Phe Thr Arg Trp Ala 290 295 300 Ala Ala Leu Ala Pro 305 <210> 5 <211> 309 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 5 Met Val Thr Ser Pro Ala Leu Arg Asp Val His Val Pro His Ala Tyr 1 5 10 15 Pro Glu Gln Gln Val Asp Leu Gly Glu Ile Thr Met Asn Tyr Ala Glu 20 25 30 Ala Gly Asp Pro Gly Arg Pro Ala Val Leu Leu Ile Pro Glu Gln Thr 35 40 45 Gly Ser Trp Trp Ser Tyr Glu Glu Ala Met Gly Leu Leu Ala Glu His 50 55 60 Phe His Val Tyr Ala Val Asp Leu Arg Gly Gln Gly Arg Ser Ser Trp 65 70 75 80 Thr Pro Lys Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg 85 90 95 Phe Met Ala Leu Val Val Arg Arg Pro Val Val Val Ala Gly Asn Ser 100 105 110 Ser Gly Gly Val Leu Ala Ala Trp Leu Ser Ala Tyr Ser Met Pro Gly 115 120 125 Gln Ile Arg Gly Val Leu Cys Glu Asp Pro Pro Phe Phe Ala Ser Glu 130 135 140 Leu Val Pro Ala His Gly His Ser Val Arg Gln Gly Ala Gly Pro Val 145 150 155 160 Phe Glu Leu Phe Arg Thr Tyr Leu Gly Asp Gln Trp Ser Val Gly Asp 165 170 175 Trp Glu Gly Phe Arg Ser Ala Ala Gly Ala Ser Ala Ser Pro Met Ala 180 185 190 Arg Ser Phe Val Ala Asp Thr Ile Pro Gln His Leu Lys Glu Tyr Asp 195 200 205 Pro Glu Trp Ala Arg Ala Phe Tyr Glu Gly Thr Val Gly Leu Asn Cys 210 215 220 Pro His Glu Arg Met Leu Asn Arg Val Asn Thr Pro Val Leu Leu Thr 225 230 235 240 His His Met Arg Gly Thr Asp Pro Glu Thr Gly Asn Leu Leu Gly Ala 245 250 255 Leu Ser Asp Glu Gln Ala Ala Gln Ala Arg Arg Leu Met Glu Ser Ala 260 265 270 Gly Val Lys Val Asp Tyr Glu Ser Val Pro Asp Ala Ser His Met Met 275 280 285 His Gln Ser Asp Pro Ala Arg Tyr Ala Glu Ile Leu Thr Pro Trp Ala 290 295 300 Ala Ala Leu Ala Pro 305 <210> 6 <211> 328 <212> PRT <213> Secuencia Artificial <220> <223> Dominio alfa/beta hidrolasa <400> 6 Met Ala Glu Glu Gly Thr Arg Ser Glu Ala Ala Asp Ala Ala Thr Gln 1 5 10 15 Ala Arg Gln Leu Pro Asp Ser Arg Asn Ile Phe Val Ser His Arg Phe 20 25 30 Pro Glu Arg Gln Val Asp Leu Gly Glu Val Val Met Asn Phe Ala Glu 35 40 45 Ala Gly Ser Pro Asp Asn Pro Ala Leu Leu Leu Leu Pro Glu Gln Thr 50 55 60 Gly Ser Trp Trp Ser Tyr Glu Pro Val Met Gly Leu Leu Ala Glu Asn 65 70 75 80 Phe His Val Phe Ala Val Asp Ile Arg Gly Gln Gly Arg Ser Thr Trp 85 90 95 Thr Pro Arg Arg Tyr Ser Leu Asp Asn Phe Gly Asn Asp Leu Val Arg 100 105 110 Phe Ile Ala Leu Val Ile Lys Arg Pro Val Val Val Ala Gly Asn Ser 115 120 125 Ser Gly Gly Leu Leu Ala Ala Trp Leu Ser Ala Tyr Ala Met Pro Gly 130 135 140 Gln Ile Arg Ala Ala Leu Cys Glu Asp Ala Pro Phe Phe Ala Ser Glu 145 150 155 160 Leu Val Pro Ala Tyr Gly His Ser Val Leu Gln Ala Ala Gly Pro Ala 165 170 175 Phe Glu Leu Tyr Arg Asp Phe Leu Gly Asp Gln Trp Ser Ile Gly Asp 180 185 190 Trp Lys Gly Phe Val Glu Ala Ala Lys Ala Ser Pro Ala Lys Ala Met 195 200 205 Gln Leu Phe Pro Thr Pro Asp Glu Ala Pro Gln Asn Leu Lys Glu Tyr 210 215 220 Asp Pro Glu Trp Gly Arg Ala Phe Phe Glu Gly Thr Val Ala Leu His 225 230 235 240 Cys Pro His Asp Arg Met Leu Ser Gln Val Lys Thr Pro Ile Leu Ile 245 250 255 Thr His His Ala Arg Thr Ile Asp Pro Glu Thr Gly Glu Leu Leu Gly 260 265 270 Ala Leu Ser Asp Leu Gln Ala Glu His Ala Gln Asp Ile Ile Arg Ser 275 280 285 Ala Gly Val Arg Val Asp Tyr Gln Ser His Pro Asp Ala Leu His Met 290 295 300 Met His Leu Phe Asp Pro Ala Arg Tyr Ala Glu Ile Leu Thr Ser Trp 305 310 315 320 Ser Ala Thr Leu Pro Ala Asn Asp 325 <210> 7 <211> 5 <212> PRT <213> Secuencia Artificial <220> <223> Motivo <220> <221> CARACTERÍSTICA_MISCELÁNEA <222> (2)..(2) <223> X es cualquier aminoácido <400> 7 Gln Xaa Ala Gly Pro 1 5 <210> 8 <211> 5 <212> PRT <213> Secuencia Artificial <220> <223> Motivo <400> 8 Glu Tyr Asp Pro Glu 1 5 <210> 9 <211> 6 <212> PRT <213> Secuencia Artificial <220> <223> Motivo <220> <221> CARACTERÍSTICA_MISCELÁNEA <222> (2)..(2) <223> X es F o Y <220> <221> CARACTERÍSTICA_MISCELÁNEA <222> (3)..(4) <223> X es cualquier aminoácido <400> 9 Gly Xaa Xaa Xaa Ala Ala 1 5 <210> 10 <211> 4 <212> PRT <213> Secuencia Artificial <220> <223> Motivo <220> <221> CARACTERÍSTICA_MISCELÁNEA <222> (3)..(3) <223> X es cualquier aminoácido <400> 10 Ala Arg Xaa Phe 1 <210> 11 <211> 4 <212> PRT <213> Secuencia Artificial <220> <223> Motivo <400> 11 Gln Leu Phe Pro 1