LISTADO DE SECUENCIAS

<110>  BRISTOL-MYERS SQUIBB COMPANY
 
<120>  FORMULACIONES DE FGF-21 

<130>  3338.133PC01/ELE/C-K

<150>  US 62/693.847
<151>  2018-07-03

<160>  3     

<170>  PatentIn versión 3.5

<210>  1
<211>  182
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  FGF-21 modificado en Q109


<220>
<221>  misc_feature
<222>  (109)..(109)
<223>  Q109 de tipo silvestre reemplazado por para-acetil-L-fenilalanina 

<400>  1

Met His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln 
1               5                   10                  15      


Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala 
            20                  25                  30          


His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln 
        35                  40                  45              


Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile 
    50                  55                  60                  


Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp 
65                  70                  75                  80  


Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe 
                85                  90                  95      


Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Xaa Ser Glu Ala 
            100                 105                 110         


His Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp 
        115                 120                 125             


Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro 
    130                 135                 140                 


Pro Ala Pro Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp 
145                 150                 155                 160 


Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg 
                165                 170                 175     


Ser Pro Ser Tyr Ala Ser 
            180         


<210>  2
<211>  182
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  FGF-21 modificado en Q109 PEGILADO PEG-FGF-21 


<220>
<221>  misc_feature
<222>  (109)..(109)
<223>  Para-acetil-L-fenilalanina unida a 30 kDa de poli(etilenglicol) por medio de un enlace oxima

<400>  2

Met His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln 
1               5                   10                  15      


Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala 
            20                  25                  30          


His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln 
        35                  40                  45              


Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile 
    50                  55                  60                  


Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp 
65                  70                  75                  80  


Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe 
                85                  90                  95      


Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Xaa Ser Glu Ala 
            100                 105                 110         


His Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp 
        115                 120                 125             


Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro 
    130                 135                 140                 


Pro Ala Pro Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp 
145                 150                 155                 160 


Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg 
                165                 170                 175     


Ser Pro Ser Tyr Ala Ser 
            180         


<210>  3
<211>  182
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Secuencia de tipo silvestre de FGF-21

<400>  3

Met His Pro Ile Pro Asp Ser Ser Pro Leu Leu Gln Phe Gly Gly Gln 
1               5                   10                  15      


Val Arg Gln Arg Tyr Leu Tyr Thr Asp Asp Ala Gln Gln Thr Glu Ala 
            20                  25                  30          


His Leu Glu Ile Arg Glu Asp Gly Thr Val Gly Gly Ala Ala Asp Gln 
        35                  40                  45              


Ser Pro Glu Ser Leu Leu Gln Leu Lys Ala Leu Lys Pro Gly Val Ile 
    50                  55                  60                  


Gln Ile Leu Gly Val Lys Thr Ser Arg Phe Leu Cys Gln Arg Pro Asp 
65                  70                  75                  80  


Gly Ala Leu Tyr Gly Ser Leu His Phe Asp Pro Glu Ala Cys Ser Phe 
                85                  90                  95      


Arg Glu Leu Leu Leu Glu Asp Gly Tyr Asn Val Tyr Gln Ser Glu Ala 
            100                 105                 110         


His Gly Leu Pro Leu His Leu Pro Gly Asn Lys Ser Pro His Arg Asp 
        115                 120                 125             


Pro Ala Pro Arg Gly Pro Ala Arg Phe Leu Pro Leu Pro Gly Leu Pro 
    130                 135                 140                 


Pro Ala Pro Pro Glu Pro Pro Gly Ile Leu Ala Pro Gln Pro Pro Asp 
145                 150                 155                 160 


Val Gly Ser Ser Asp Pro Leu Ser Met Val Gly Pro Ser Gln Gly Arg 
                165                 170                 175     


Ser Pro Ser Tyr Ala Ser 
            180