LISTADO DE SECUENCIAS <110> The Trustees of The University of Pennsylvania <120> COMPOSICIONES PARA LA REDUCCIÓN DE LA EXPRESIÓN TRANSGÉNICA ESPECÍFICA DE DRG <130> 18-8514PCT <150> US 62/783,956 <151> 21-12-2018 <150> US 62/924,970 <151> 23-10-2019 <150> US 662/934,915 <151> 13-11-2019 <160> 17 <170> PatentIn versión 3.5 <210> 1 <211> 22 <212> ADN <213> Secuencia artificial <220> <223> diana de miR-183 <400> 1 agtgaattct accagtgcca ta 22 <210> 2 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> diana de mirR-96 <400> 2 agcaaaaatg tgctagtgcc aaa 23 <210> 3 <211> 24 <212> ADN <213> Secuencia artificial <220> <223> diana de miR-182 <400> 3 agtgtgagtt ctaccattgc caaa 24 <210> 4 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> diana de miR-145 <400> 4 agggattcct gggaaaactg gac 23 <210> 5 <211> 4 <212> ADN <213> Secuencia artificial <220> <223> Espaciador (i) <400> 5 ggat 4 <210> 6 <211> 6 <212> ADN <213> Secuencia artificial <220> <223> Espaciador (ii) <400> 6 cacgtg 6 <210> 7 <211> 6 <212> ADN <213> Secuencia artificial <220> <223> espaciador iii <400> 7 gcatgc 6 <210> 8 <211> 2211 <212> ADN <213> virus adenoasociado hu68 <220> <221> CDS <222> (1)..(2211) <400> 8 atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctc agt 48 Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 1 5 10 15 gaa ggc att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc 96 Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 20 25 30 aag gca aat caa caa cat caa gac aac gct cgg ggt ctt gtg ctt ccg 144 Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 35 40 45 ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg 192 Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 50 55 60 gtc aac gaa gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac 240 Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 65 70 75 80 cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc 288 Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 85 90 95 gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc 336 Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 100 105 110 aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct 384 Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 115 120 125 ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg 432 Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 130 135 140 cct gta gag cag tct cct cag gaa ccg gac tcc tcc gtg ggt att ggc 480 Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 145 150 155 160 aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act 528 Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 165 170 175 ggc gac aca gag tca gtc ccc gac cct caa cca atc gga gaa cct ccc 576 Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 180 185 190 gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc 624 Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 195 200 205 gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc 672 Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 210 215 220 tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc 720 Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 225 230 235 240 acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc 768 Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 245 250 255 tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac 816 Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 260 265 270 gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga 864 Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 275 280 285 ttc cac tgc cac ttc tca cca cgt gac tgg caa aga ctc atc aac aac 912 Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 290 295 300 aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att 960 Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 305 310 315 320 cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gct aat 1008 Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 325 330 335 aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc 1056 Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 340 345 350 ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca 1104 Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 355 360 365 gcg gac gtt ttc atg att cct cag tac ggg tat cta acg ctt aat gat 1152 Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 370 375 380 gga agc caa gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc 1200 Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 385 390 395 400 ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag 1248 Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 405 410 415 ttt gag aac gta cct ttc cat agc agc tat gct cac agc caa agc ctg 1296 Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 420 425 430 gac cga ctc atg aat cca ctc atc gac caa tac ttg tac tat ctc tca 1344 Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 435 440 445 aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc agt 1392 Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 450 455 460 gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata cct 1440 Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 465 470 475 480 gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa aac 1488 Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 485 490 495 aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc aat 1536 Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 500 505 510 gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac aaa 1584 Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 515 520 525 gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt ggc 1632 Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 530 535 540 aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg ata 1680 Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 545 550 555 560 acc aac gaa gaa gaa att aaa act acc aac cca gta gca acg gag tcc 1728 Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 565 570 575 tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg cag 1776 Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 580 585 590 acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg cag 1824 Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 595 600 605 gac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct cac 1872 Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 610 615 620 acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga atg 1920 Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 625 630 635 640 aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct gcg 1968 Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 645 650 655 gat cct cca acg gct ttc aac aag gac aag ctg aac tct ttc atc acc 2016 Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 660 665 670 cag tat tct act ggc caa gtc agc gtg gag att gag tgg gag ctg cag 2064 Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 675 680 685 aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc aac 2112 Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 690 695 700 tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt gtt 2160 Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 705 710 715 720 tat tct gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat ctg 2208 Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 725 730 735 taa 2211 <210> 9 <211> 736 <212> PRT <213> virus adenoasociado hu68 <400> 9 Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 1 5 10 15 Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 20 25 30 Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 35 40 45 Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 50 55 60 Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 65 70 75 80 Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 85 90 95 Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 100 105 110 Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 115 120 125 Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 130 135 140 Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Val Gly Ile Gly 145 150 155 160 Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 165 170 175 Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 180 185 190 Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 195 200 205 Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 210 215 220 Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 225 230 235 240 Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 245 250 255 Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 260 265 270 Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 275 280 285 Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 290 295 300 Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 305 310 315 320 Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 325 330 335 Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 340 345 350 Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 355 360 365 Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 370 375 380 Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 385 390 395 400 Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 405 410 415 Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 420 425 430 Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 435 440 445 Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 450 455 460 Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 465 470 475 480 Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 485 490 495 Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 500 505 510 Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 515 520 525 Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 530 535 540 Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 545 550 555 560 Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 565 570 575 Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 580 585 590 Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 595 600 605 Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 610 615 620 Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 625 630 635 640 Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 645 650 655 Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 660 665 670 Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 675 680 685 Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 690 695 700 Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 705 710 715 720 Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 725 730 735 <210> 10 <211> 3198 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.CI.eGFP.miR145.rBG.ITR <220> <221> repeat_region <222> (1)..(130) <223> 5' - AAV2 - ITR <220> <221> misc_feature <222> (1)..(130) <223> 5' - AAV2 - ITR <220> <221> promotor <222> (198)..(579) <223> promotor de CMV IE <220> <221> promotor <222> (582)..(863) <223> promotor de CB <220> <221> misc_feature <222> (1979)..(2698) <223> gen eGFP <220> <221> misc_feature <222> (2705)..(2727) <223> miR145 <220> <221> misc_feature <222> (2728)..(2731) <223> espacidor <220> <221> misc_feature <222> (2732)..(2754) <223> miR145 <220> <221> misc_feature <222> (2755)..(2760) <223> espacidor <220> <221> misc_feature <222> (2761)..(2783) <223> miR145 <220> <221> misc_feature <222> (2784)..(2789) <223> espacidor <220> <221> misc_feature <222> (2790)..(2812) <223> miR145 <220> <221> misc_feature <222> (2981)..(3198) <223> ITR 3' <400> 10 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattacttaa tacgactcac tataggctag taatacgact cactatagat 1980 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2040 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2100 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2160 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 2220 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2280 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 2340 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 2400 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 2460 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 2520 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2580 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2640 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg 2700 taccagggat tcctgggaaa actggacgga tagggattcc tgggaaaact ggaccacgtg 2760 agggattcct gggaaaactg gacgcatgca gggattcctg ggaaaactgg acgcggccgc 2820 ctcgaggacg gggtgaacta cgcctgagga tccgatcttt ttccctctgc caaaaattat 2880 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 2940 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaagcaattc gttgatctga 3000 atttcgacca cccataatac ccattaccct ggtagataag tagcatggcg ggttaatcat 3060 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 3120 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 3180 gagcgagcga gcgcgcag 3198 <210> 11 <211> 3202 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.CI.eGFP.miR182.rGB.ITR <220> <221> misc_feature <222> (1)..(130) <223> ITR 5' (AAV2) <220> <221> misc_feature <222> (198)..(579) <223> promotor de CMV IE <220> <221> misc_feature <222> (582)..(863) <223> promotor de CB <220> <221> misc_feature <222> (958)..(1930) <223> intrón de beta-actina de pollo <220> <221> misc_feature <222> (1979)..(2698) <223> secuencia codificante de eGFP <220> <221> misc_feature <222> (2705)..(2728) <223> miR182 <220> <221> misc_feature <222> (2729)..(2732) <223> espaciador <220> <221> misc_feature <222> (2733)..(2756) <223> miR182 <220> <221> misc_feature <222> (2757)..(2760) <223> espaciador <220> <221> misc_feature <222> (2763)..(2786) <223> miR182 <220> <221> misc_feature <222> (2787)..(2792) <223> espaciador <220> <221> misc_feature <222> (2793)..(2816) <223> miR182 <220> <221> polyA_signal <222> (2858)..(2984) <220> <221> misc_feature <222> (3073)..(3202) <223> ITR 3' <400> 11 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattacttaa tacgactcac tataggctag taatacgact cactatagat 1980 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2040 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2100 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2160 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 2220 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2280 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 2340 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 2400 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 2460 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 2520 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2580 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2640 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg 2700 taccagtgtg agttctacca ttgccaaagg atagtgtgag ttctaccatt gccaaacacg 2760 tgagtgtgag ttctaccatt gccaaagcat gcagtgtgag ttctaccatt gccaaagcgg 2820 ccgcctcgag gacggggtga actacgcctg aggatccgat ctttttccct ctgccaaaaa 2880 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 2940 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagca attcgttgat 3000 ctgaatttcg accacccata atacccatta ccctggtaga taagtagcat ggcgggttaa 3060 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct 3120 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct 3180 cagtgagcga gcgagcgcgc ag 3202 <210> 12 <211> 3198 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.eGFP.miARN96.rBG.ITR <220> <221> misc_feature <222> (1979)..(2699) <223> secuencia codificante de eGFP <220> <221> misc_feature <222> (2705)..(2727) <223> miR96 <220> <221> misc_feature <222> (2728)..(2731) <223> espaciador <220> <221> misc_feature <222> (2732)..(2754) <223> miR96 <220> <221> misc_feature <222> (2755)..(2760) <223> espaciador <220> <221> misc_feature <222> (2761)..(2783) <223> miR96 <220> <221> misc_feature <222> (2784)..(2789) <223> espaciador <220> <221> misc_feature <222> (2790)..(2812) <223> miR96 <400> 12 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattacttaa tacgactcac tataggctag taatacgact cactatagat 1980 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2040 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2100 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2160 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 2220 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2280 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 2340 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 2400 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 2460 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 2520 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2580 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2640 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg 2700 taccagcaaa aatgtgctag tgccaaagga tagcaaaaat gtgctagtgc caaacacgtg 2760 agcaaaaatg tgctagtgcc aaagcatgca gcaaaaatgt gctagtgcca aagcggccgc 2820 ctcgaggacg gggtgaacta cgcctgagga tccgatcttt ttccctctgc caaaaattat 2880 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc 2940 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaagcaattc gttgatctga 3000 atttcgacca cccataatac ccattaccct ggtagataag tagcatggcg ggttaatcat 3060 taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 3120 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 3180 gagcgagcga gcgcgcag 3198 <210> 13 <211> 3194 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.CI.eGFP.miARN183.rBG.ITR <220> <221> misc_feature <222> (1979)..(2698) <223> secuencia codificante de eGFP <220> <221> misc_feature <222> (2705)..(2726) <223> miARN183 <220> <221> misc_feature <222> (2727)..(2730) <223> espaciador <220> <221> misc_feature <222> (2731)..(2752) <223> miARN183 <220> <221> misc_feature <222> (2753)..(2758) <223> espaciador <220> <221> misc_feature <222> (2781)..(2786) <223> espaciador <220> <221> misc_feature <222> (2787)..(2808) <223> miARN183 <400> 13 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattacttaa tacgactcac tataggctag taatacgact cactatagat 1980 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 2040 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 2100 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 2160 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca 2220 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 2280 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 2340 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 2400 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg 2460 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga 2520 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2580 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2640 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg 2700 taccagtgaa ttctaccagt gccataggat agtgaattct accagtgcca tacacgtgag 2760 tgaattctac cagtgccata gcatgcagtg aattctacca gtgccatagc ggccgcctcg 2820 aggacggggt gaactacgcc tgaggatccg atctttttcc ctctgccaaa aattatgggg 2880 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 2940 caatagtgtg ttggaatttt ttgtgtctct cactcggaag caattcgttg atctgaattt 3000 cgaccaccca taatacccat taccctggta gataagtagc atggcgggtt aatcattaac 3060 tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 3120 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 3180 gagcgagcgc gcag 3194 <210> 14 <211> 4300 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.CI.hIDUAcoV1.rBG.ITR <400> 14 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattcagcca ccatgaggcc tctcagacct agagctgctc tgctggcact 1980 gctggcttct ctgcttgctg ctcctcctgt ggctcctgcc gaagctcctc atctggtgca 2040 cgtggatgcc gccagagcac tgtggcccct gagaagattt tggcggagca ccggcttttg 2100 ccctccactg cctcattctc aggccgacca gtacgtgctg agctgggacc agcaactgaa 2160 cctggcctac gtgggagccg tgcctcacag aggcattaag caagtgcgga cccactggct 2220 gctggaactg gtcacaacaa gaggcagcac aggcagaggc ctgagctaca acttcaccca 2280 cctggacggc tacctggacc tgctgagaga gaatcagctg ctgcctggct tcgagctgat 2340 gggctctgcc tctggccact tcaccgactt cgaggacaag cagcaggttt tcgagtggaa 2400 ggacctggtg tccagcctgg ccagacggta catcggcaga tacggactgg cccacgtgtc 2460 caagtggaac ttcgagacct ggaacgagcc cgaccaccac gacttcgaca acgtgtcaat 2520 gaccatgcag ggctttctga actactacga cgcctgcagc gagggcctga gagctgcttc 2580 tcctgctctg agacttggcg gccctggcga ctcttttcac acccctccaa gaagccctct 2640 gtcctgggga ctgctgagac actgtcacga cggcaccaat ttcttcaccg gcgaggctgg 2700 cgtgcggctg gattatatca gcctgcacag aaagggcgcc agaagcagca tcagcatcct 2760 ggaacaagag aaggtggtgg cccagcagat cagacagctg ttccccaagt tcgccgacac 2820 acccatctac aacgacgagg ccgatcctct cgttggctgg tcacttcctc agccttggag 2880 agccgatgtg acctatgccg ccatggtggt caaagtgatc gcccagcacc agaatctgct 2940 gctcgccaat accaccagcg cctttccata cgctctgctg agcaacgaca acgccttcct 3000 gagctatcac cctcatcctt tcgctcagcg gaccctgacc gccagattcc aagtgaacaa 3060 cacccggcct ccacacgtgc agctgctgag aaaaccagtg ctgacagcca tgggcctgct 3120 cgccctgctg gacgaagaac aactgtgggc cgaagtgtcc caggccggaa cagtgctgga 3180 tagcaatcac acagtgggcg tgctggcctc cgctcataga cctcaaggac cagccgatgc 3240 ttggagggct gccgtgctga tctacgccag cgacgataca agggctcacc ccaacagatc 3300 cgtggccgtg acactgagac tgagaggcgt tccaccagga cctggcctgg tgtacgtgac 3360 cagatacctg gacaacggcc tgtgcagccc tgatggcgaa tggcgtagac taggcagacc 3420 tgtgtttcct accgccgagc agttcagacg gatgagagcc gctgaagatc ccgtggctgc 3480 tgctccaaga cctcttccag ctggtggcag actgactctg aggcctgcac tcagactgcc 3540 tagtctgctg ctggtgcacg tctgtgccag acctgagaag cctcctggcc aagtgacaag 3600 actgagggcc ctgccactga cacagggaca gctggttctt gtttggagcg acgagcacgt 3660 gggcagcaag tgtctgtgga cctacgagat ccagttcagc caggacggca aggcctacac 3720 acccgtgtct agaaagccta gcaccttcaa cctgttcgtg ttcagccccg atacaggcgc 3780 cgtgtctggc agctatagag tcagagccct ggactactgg gccagaccag gaccattttc 3840 tgaccccgtg ccttacctgg aagtgcccgt tcctagaggc cctccttctc ctggaaatcc 3900 ctgataaggt accataccta ggctcgagga cggggtgaac tacgcctgag gatccgatct 3960 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 4020 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 4080 cggaagcaat tcgttgatct gaatttcgac cacccataat acccattacc ctggtagata 4140 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc 4200 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 4260 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag 4300 <210> 15 <211> 4403 <212> ADN <213> Secuencia artificial <220> <223> ITR.CB7.CI.hIDUAcoV1.miR183.ITR <220> <221> misc_feature <222> (1938)..(3908) <223> hIDUAcoV1 <220> <221> misc_feature <222> (3915)..(3936) <223> miARN183 <220> <221> misc_feature <222> (3937)..(3940) <223> espaciador <220> <221> misc_feature <222> (3941)..(3962) <223> miARN183 <220> <221> misc_feature <222> (3963)..(3968) <223> espaciador <220> <221> misc_feature <222> (3969)..(3990) <223> miARN183 <220> <221> misc_feature <222> (3991)..(3996) <223> espaciador <220> <221> misc_feature <222> (3997)..(4018) <223> miARN183 <400> 15 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt 60 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120 aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg 180 atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat 240 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 300 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 360 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 420 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 480 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 540 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac 600 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat 660 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg 720 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca 780 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa 840 aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc 900 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 960 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 1020 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 1080 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 1140 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 1200 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 1260 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 1320 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 1380 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 1440 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg 1500 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 1560 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 1620 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 1680 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 1740 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 1800 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 1860 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 1920 tttggcaaag aattcagcca ccatgaggcc tctcagacct agagctgctc tgctggcact 1980 gctggcttct ctgcttgctg ctcctcctgt ggctcctgcc gaagctcctc atctggtgca 2040 cgtggatgcc gccagagcac tgtggcccct gagaagattt tggcggagca ccggcttttg 2100 ccctccactg cctcattctc aggccgacca gtacgtgctg agctgggacc agcaactgaa 2160 cctggcctac gtgggagccg tgcctcacag aggcattaag caagtgcgga cccactggct 2220 gctggaactg gtcacaacaa gaggcagcac aggcagaggc ctgagctaca acttcaccca 2280 cctggacggc tacctggacc tgctgagaga gaatcagctg ctgcctggct tcgagctgat 2340 gggctctgcc tctggccact tcaccgactt cgaggacaag cagcaggttt tcgagtggaa 2400 ggacctggtg tccagcctgg ccagacggta catcggcaga tacggactgg cccacgtgtc 2460 caagtggaac ttcgagacct ggaacgagcc cgaccaccac gacttcgaca acgtgtcaat 2520 gaccatgcag ggctttctga actactacga cgcctgcagc gagggcctga gagctgcttc 2580 tcctgctctg agacttggcg gccctggcga ctcttttcac acccctccaa gaagccctct 2640 gtcctgggga ctgctgagac actgtcacga cggcaccaat ttcttcaccg gcgaggctgg 2700 cgtgcggctg gattatatca gcctgcacag aaagggcgcc agaagcagca tcagcatcct 2760 ggaacaagag aaggtggtgg cccagcagat cagacagctg ttccccaagt tcgccgacac 2820 acccatctac aacgacgagg ccgatcctct cgttggctgg tcacttcctc agccttggag 2880 agccgatgtg acctatgccg ccatggtggt caaagtgatc gcccagcacc agaatctgct 2940 gctcgccaat accaccagcg cctttccata cgctctgctg agcaacgaca acgccttcct 3000 gagctatcac cctcatcctt tcgctcagcg gaccctgacc gccagattcc aagtgaacaa 3060 cacccggcct ccacacgtgc agctgctgag aaaaccagtg ctgacagcca tgggcctgct 3120 cgccctgctg gacgaagaac aactgtgggc cgaagtgtcc caggccggaa cagtgctgga 3180 tagcaatcac acagtgggcg tgctggcctc cgctcataga cctcaaggac cagccgatgc 3240 ttggagggct gccgtgctga tctacgccag cgacgataca agggctcacc ccaacagatc 3300 cgtggccgtg acactgagac tgagaggcgt tccaccagga cctggcctgg tgtacgtgac 3360 cagatacctg gacaacggcc tgtgcagccc tgatggcgaa tggcgtagac taggcagacc 3420 tgtgtttcct accgccgagc agttcagacg gatgagagcc gctgaagatc ccgtggctgc 3480 tgctccaaga cctcttccag ctggtggcag actgactctg aggcctgcac tcagactgcc 3540 tagtctgctg ctggtgcacg tctgtgccag acctgagaag cctcctggcc aagtgacaag 3600 actgagggcc ctgccactga cacagggaca gctggttctt gtttggagcg acgagcacgt 3660 gggcagcaag tgtctgtgga cctacgagat ccagttcagc caggacggca aggcctacac 3720 acccgtgtct agaaagccta gcaccttcaa cctgttcgtg ttcagccccg atacaggcgc 3780 cgtgtctggc agctatagag tcagagccct ggactactgg gccagaccag gaccattttc 3840 tgaccccgtg ccttacctgg aagtgcccgt tcctagaggc cctccttctc ctggaaatcc 3900 ctgataaggt accagtgaat tctaccagtg ccataggata gtgaattcta ccagtgccat 3960 acacgtgagt gaattctacc agtgccatag catgcagtga attctaccag tgccatagcg 4020 gccgcctcga ggacggggtg aactacgcct gaggatccga tctttttccc tctgccaaaa 4080 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta 4140 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagc aattcgttga 4200 tctgaatttc gaccacccat aatacccatt accctggtag ataagtagca tggcgggtta 4260 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 4320 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 4380 tcagtgagcg agcgagcgcg cag 4403 <210> 16 <211> 2211 <212> ADN <213> virus adenoasociado 9 <220> <221> CDS <222> (1)..(2211) <400> 16 atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctt agt 48 Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 1 5 10 15 gaa gga att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc 96 Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 20 25 30 aag gca aat caa caa cat caa gac aac gct cga ggt ctt gtg ctt ccg 144 Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 35 40 45 ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg 192 Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 50 55 60 gtc aac gca gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac 240 Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 65 70 75 80 cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc 288 Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 85 90 95 gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc 336 Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 100 105 110 aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct 384 Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 115 120 125 ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg 432 Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 130 135 140 cct gta gag cag tct cct cag gaa ccg gac tcc tcc gcg ggt att ggc 480 Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 145 150 155 160 aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act 528 Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 165 170 175 ggc gac aca gag tca gtc cca gac cct caa cca atc gga gaa cct ccc 576 Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 180 185 190 gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc 624 Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 195 200 205 gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc 672 Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 210 215 220 tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc 720 Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 225 230 235 240 acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc 768 Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 245 250 255 tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac 816 Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 260 265 270 gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga 864 Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 275 280 285 ttc cac tgc cac ttc tca cca cgt gac tgg cag cga ctc atc aac aac 912 Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 290 295 300 aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att 960 Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 305 310 315 320 cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gcc aat 1008 Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 325 330 335 aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc 1056 Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 340 345 350 ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca 1104 Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 355 360 365 gcg gac gtt ttc atg att cct cag tac ggg tat ctg acg ctt aat gat 1152 Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 370 375 380 gga agc cag gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc 1200 Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 385 390 395 400 ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag 1248 Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 405 410 415 ttt gag aac gta cct ttc cat agc agc tac gct cac agc caa agc ctg 1296 Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 420 425 430 gac cga cta atg aat cca ctc atc gac caa tac ttg tac tat ctc tca 1344 Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 435 440 445 aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc agt 1392 Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 450 455 460 gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata cct 1440 Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 465 470 475 480 gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa aac 1488 Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 485 490 495 aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc aat 1536 Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 500 505 510 gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac aaa 1584 Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 515 520 525 gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt ggc 1632 Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 530 535 540 aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg ata 1680 Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 545 550 555 560 acc aac gaa gaa gaa att aaa act act aac ccg gta gca acg gag tcc 1728 Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 565 570 575 tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg cag 1776 Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 580 585 590 acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg cag 1824 Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 595 600 605 gac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct cac 1872 Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 610 615 620 acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga atg 1920 Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 625 630 635 640 aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct gcg 1968 Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 645 650 655 gat cct cca acg gcc ttc aac aag gac aag ctg aac tct ttc atc acc 2016 Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 660 665 670 cag tat tct act ggc caa gtc agc gtg gag atc gag tgg gag ctg cag 2064 Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 675 680 685 aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc aac 2112 Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 690 695 700 tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt gta 2160 Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 705 710 715 720 tat agt gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat ctg 2208 Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 725 730 735 taa 2211 <210> 17 <211> 736 <212> PRT <213> virus adenoasociado 9 <400> 17 Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 1 5 10 15 Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 20 25 30 Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 35 40 45 Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 50 55 60 Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 65 70 75 80 Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 85 90 95 Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 100 105 110 Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 115 120 125 Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 130 135 140 Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 145 150 155 160 Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 165 170 175 Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 180 185 190 Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 195 200 205 Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 210 215 220 Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 225 230 235 240 Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 245 250 255 Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 260 265 270 Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 275 280 285 Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 290 295 300 Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 305 310 315 320 Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 325 330 335 Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 340 345 350 Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 355 360 365 Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 370 375 380 Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 385 390 395 400 Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 405 410 415 Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 420 425 430 Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 435 440 445 Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 450 455 460 Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 465 470 475 480 Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 485 490 495 Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 500 505 510 Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 515 520 525 Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 530 535 540 Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 545 550 555 560 Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 565 570 575 Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 580 585 590 Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 595 600 605 Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 610 615 620 Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 625 630 635 640 Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 645 650 655 Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 660 665 670 Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 675 680 685 Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 690 695 700 Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 705 710 715 720 Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 725 730 735