LISTADO DE SECUENCIAS <110> BIOVERATIV THERAPEUTICS INC. <120> VECTORES LENTIVIRALES QUE EXPRESAN EL FACTOR IX <130> SA9-468PC <150> US 62/776.393 <151> 06-12-2018 <160> 27 <170> PatentIn versión 3.5 <210> 1 <211> 1248 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-1-R338L <400> 1 tacaatagtg ggaagctgga ggaatttgtg cagggcaacc tggagagaga atgcatggag 60 gaaaagtgta gcttcgagga agcccgcgag gtgtttgaaa atacagagcg aaccacagag 120 ttctggaagc agtatgtgga cggcgatcag tgcgagagca acccctgtct gaatggcgga 180 agttgcaaag acgatatcaa ctcatacgaa tgctggtgtc ctttcgggtt tgaaggcaaa 240 aattgcgagc tggacgtgac atgtaacatt aagaatggac ggtgcgagca gttttgtaaa 300 aactctgccg ataataaggt ggtgtgcagc tgtactgaag gatatcgcct ggctgagaac 360 cagaagtcct gcgaaccagc agtgcccttc ccttgtggga gggtgagcgt ctcccagact 420 tcaaaactga ccagagcaga gacagtgttt cccgacgtgg attacgtcaa cagcactgag 480 gccgaaacca tcctggacaa cattactcag tctacccaga gtttcaatga ctttactcgg 540 gtggtcgggg gcgaggatgc taaaccaggc cagttcccct ggcaggtggt cctgaacgga 600 aaggtggatg cattttgcgg agggtctatc gtgaatgaga aatggattgt caccgccgct 660 cactgcgtgg aaaccggagt caagatcaca gtggtcgctg gggagcacaa cattgaggaa 720 acagaacata ctgagcagaa gcggaatgtg atccgcatca ttcctcacca taactacaat 780 gcagccatca acaaatacaa tcatgacatt gccctgctgg aactggatga gcctctggtg 840 ctgaacagct acgtcactcc aatctgcatt gctgacaaag agtataccaa tatcttcctg 900 aagtttggat cagggtacgt gagcggctgg ggaagagtct tccacaaggg caggagcgcc 960 ctggtgctcc agtatctgcg agtgcctctg gtcgatcgag ctacctgtct gctctctacc 1020 aagtttacaa tctacaacaa catgttctgc gctgggtttc acgagggagg acgagactcc 1080 tgtcagggcg attctggggg cccacatgtg acagaggtcg aaggcaccag cttcctgact 1140 ggcatcattt cctggggaga ggaatgtgca atgaagggaa aatacgggat ctacaccaaa 1200 gtgagccgct atgtgaactg gatcaaggaa aaaaccaaac tgacctaa 1248 <210> 2 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-1b-R338L <400> 2 atgcagagag tcaacatgat tatggctgag tcacctgggc tgattactat ttgcctgctg 60 ggctacctgc tgtccgccga gtgtaccgtg ttcctggacc atgagaacgc aaataagatc 120 ctgaacaggc ccaaaagata caatagtggg aagctggagg aatttgtgca gggcaacctg 180 gagagagaat gcatggagga aaagtgtagc ttcgaggaag cccgcgaggt gtttgaaaat 240 acagagcgaa ccacagagtt ctggaagcag tatgtggacg gcgatcagtg cgagagcaac 300 ccctgtctga atggcggaag ttgcaaagac gatatcaact catacgaatg ctggtgtcct 360 ttcgggtttg aaggcaaaaa ttgcgagctg gacgtgacat gtaacattaa gaatggacgg 420 tgcgagcagt tttgtaaaaa ctctgccgat aataaggtgg tgtgcagctg tactgaagga 480 tatcgcctgg ctgagaacca gaagtcctgc gaaccagcag tgcccttccc ttgtgggagg 540 gtgagcgtct cccagacttc aaaactgacc agagcagaga cagtgtttcc cgacgtggat 600 tacgtcaaca gcactgaggc cgaaaccatc ctggacaaca ttactcagtc tacccagagt 660 ttcaatgact ttactcgggt ggtcgggggc gaggatgcta aaccaggcca gttcccctgg 720 caggtggtcc tgaacggaaa ggtggatgca ttttgcggag ggtctatcgt gaatgagaaa 780 tggattgtca ccgccgctca ctgcgtggaa accggagtca agatcacagt ggtcgctggg 840 gagcacaaca ttgaggaaac agaacatact gagcagaagc ggaatgtgat ccgcatcatt 900 cctcaccata actacaatgc agccatcaac aaatacaatc atgacattgc cctgctggaa 960 ctggatgagc ctctggtgct gaacagctac gtcactccaa tctgcattgc tgacaaagag 1020 tataccaata tcttcctgaa gtttggatca gggtacgtga gcggctgggg aagagtcttc 1080 cacaagggca ggagcgccct ggtgctccag tatctgcgag tgcctctggt cgatcgagct 1140 acctgtctgc tgtctaccaa gtttacaatc tacaacaaca tgttctgcgc tgggtttcac 1200 gagggaggac gagactcctg tcagggcgat tctgggggcc cacatgtgac agaggtcgaa 1260 ggcaccagct tcctgactgg catcatttcc tggggagagg aatgtgcaat gaagggaaaa 1320 tacgggatct acaccaaagt gagccgctat gtgaactgga tcaaggaaaa aaccaaactg 1380 acctaa 1386 <210> 3 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-2-R338L <400> 3 atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg 60 ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc 120 ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg 180 gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac 240 accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac 300 ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc 360 ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg 420 tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc 480 tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg 540 gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac 600 tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc 660 ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcccggcca gttcccctgg 720 caggtcgtgc tgaatggcaa agtcgatgcc ttctgcgggg gcagcatcgt caacgagaag 780 tggattgtga ctgccgccca ttgcgtggaa accggggtga agatcactgt ggtggctggg 840 gagcacaaca tcgaggaaac cgagcacacc gagcagaaga ggaacgtgat caggattatc 900 ccccatcaca actacaatgc cgccatcaat aagtacaacc atgatattgc cctgctggag 960 ctggatgaac ccctggtcct gaacagctat gtgactccca tctgcattgc cgacaaggag 1020 tataccaaca tcttcctgaa atttggcagc ggctatgtct ctggctgggg cagggtgttc 1080 cataagggga ggagcgccct ggtcctgcag tacctgagag tgcccctggt ggacagggcc 1140 acctgcctgc tgagcaccaa gttcaccatc tacaacaata tgttttgcgc tggcttccat 1200 gaggggggca gggacagctg ccagggggac agcgggggcc cccatgtgac tgaggtggag 1260 ggcaccagct tcctgaccgg catcatcagc tggggggagg agtgcgccat gaaggggaag 1320 tatggcatct acaccaaagt ctccagatac gtcaactgga tcaaggagaa gaccaagctg 1380 acctaa 1386 <210> 4 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-3-R338L <400> 4 atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg 60 ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc 120 ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg 180 gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac 240 accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac 300 ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc 360 ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg 420 tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc 480 tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg 540 gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac 600 tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc 660 ttcaacgact tcaccagggt ggtgggcggc gaggacgcca agcccggcca gtttccctgg 720 caggtggtgc tgaacggcaa ggtggacgcc ttctgcggcg gcagcatcgt gaacgagaag 780 tggatcgtga ccgctgccca ttgcgtggaa accggcgtga agatcaccgt ggtggccggc 840 gagcacaaca tcgaagagac cgagcacacc gaacagaaaa ggaacgtgat caggatcatc 900 cctcaccata actacaatgc cgccattaac aagtacaatc acgacatcgc tctgctggaa 960 ctggatgaac ccctggtgct gaacagctac gtgaccccta tctgcatcgc cgacaaggag 1020 tatactaaca tctttctgaa gtttggcagc ggctatgtga gcggctgggg cagggtgttc 1080 cacaaaggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggatagggct 1140 acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgtgc cggcttccac 1200 gaaggcggca gggactcttg ccagggcgac agcggcggcc cccatgtgac cgaggtggaa 1260 ggcactagct ttctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag 1320 tacggcatct acactaaggt gagcaggtac gtgaactgga tcaaagaaaa gaccaagctg 1380 acctaa 1386 <210> 5 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-4-R338L <400> 5 atgcagaggg tgaacatgat catggccgag agccccggcc tgatcaccat ctgcctgctg 60 ggctacctgc tgagcgccga gtgcaccgtg ttcctggacc acgagaacgc caacaagatc 120 ctgaacaggc ccaagaggta caacagcggc aagctggagg agttcgtgca gggcaacctg 180 gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gttcgagaac 240 accgagagga ccaccgagtt ctggaagcag tacgtggacg gcgaccagtg cgagagcaac 300 ccctgcctga acggcggcag ctgcaaggac gacatcaaca gctacgagtg ctggtgcccc 360 ttcggcttcg agggcaagaa ctgcgagctg gacgtgacct gcaacatcaa gaacggcagg 420 tgcgagcagt tctgcaagaa cagcgccgac aacaaggtgg tgtgcagctg caccgagggc 480 tacaggctgg ccgagaacca gaagagctgc gagcccgccg tgcccttccc ctgcggcagg 540 gtgagcgtga gccagaccag caagctgacc agggccgaga ccgtgttccc cgacgtggac 600 tacgtgaaca gcaccgaggc cgagaccatc ctggacaaca tcacccagag cacccagagc 660 ttcaacgact tcaccagggt ggtgggcggc gaggacgcca agcccggcca gttcccctgg 720 caggtggtgc tgaacggcaa ggtggacgcc ttctgcggcg gcagcatcgt gaacgagaag 780 tggatcgtga ccgccgccca ctgcgtggag accggcgtga agatcaccgt ggtggccggc 840 gagcacaaca tcgaggagac cgagcacacc gagcagaaga ggaacgtgat caggatcatc 900 ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag 960 ctggacgagc ccctggtgct gaacagctac gtgaccccca tctgcatcgc cgacaaggag 1020 tacaccaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc 1080 cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc 1140 acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac 1200 gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag 1260 ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag 1320 tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg 1380 acctaa 1386 <210> 6 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-5-R338L <400> 6 atgcagaggg tcaacatgat catggctgag tctcctggcc tgatcaccat ctgcctgctg 60 ggctatctgc tgtccgctga gtgcactgtc ttcctggacc acgagaacgc caacaagatc 120 ctgaacaggc ccaagaggta taactctggc aagctggagg agtttgtgca ggggaacctg 180 gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gtttgagaac 240 actgagagga ccaccgagtt ctggaagcag tatgtggacg gggaccagtg cgagtctaac 300 ccttgcctga acgggggcag ctgcaaggat gacatcaaca gctatgagtg ctggtgccct 360 ttcggcttcg agggcaagaa ctgcgagctg gatgtgacct gcaacatcaa gaacggcagg 420 tgcgagcagt tctgcaagaa ctctgccgac aacaaggtgg tgtgcagctg cactgagggc 480 tataggctgg ctgagaacca gaagagctgt gagcctgctg tgcccttccc ctgcggcaga 540 gtgtctgtga gccagaccag caagctgacc agagctgaga ctgtcttccc cgacgtggac 600 tatgtgaaca gcaccgaggc tgagaccatc ctggacaaca tcacccagtc tacccagtct 660 ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcctggcca gttcccctgg 720 caggtcgtgc tgaacggcaa agtggacgcc ttctgcgggg gcagcatcgt caacgagaag 780 tggatcgtga ctgctgctca ctgcgtggaa accggggtga agatcaccgt ggtggccggc 840 gagcacaaca tcgaggagac cgagcacacc gagcagaaga ggaacgtgat caggatcatc 900 ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag 960 ctggacgagc ccctggtgct gaacagctac gtgaccccca tctgcatcgc cgacaaggag 1020 tacaccaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc 1080 cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc 1140 acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac 1200 gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag 1260 ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag 1320 tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg 1380 acctaa 1386 <210> 7 <211> 1386 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-6-R338L <400> 7 atgcagaggg tcaacatgat catggctgag tctcctggcc tgatcactat ctgcctgctg 60 ggctacctgc tgagcgccga gtgcactgtc ttcctggacc acgagaacgc caacaagatc 120 ctgaacaggc ccaagagata caactctggc aagctggagg agtttgtgca ggggaacctg 180 gagagggagt gcatggagga gaagtgcagc ttcgaggagg ccagggaggt gtttgagaac 240 actgagagga ccactgagtt ctggaagcag tatgtggacg gggaccagtg cgagtctaac 300 ccttgcctga acgggggcag ctgcaaggat gacatcaaca gctacgagtg ctggtgccct 360 ttcggcttcg agggcaagaa ctgcgagctg gatgtgactt gcaacatcaa gaacggcagg 420 tgcgagcagt tctgcaagaa ctctgccgac aacaaagtcg tgtgcagctg cactgagggc 480 tacagactgg ctgagaacca gaagagctgt gagcctgctg tgcccttccc ctgcggcaga 540 gtgtctgtga gccagaccag caagctgacc agagccgaaa ccgtgttccc cgacgtggac 600 tatgtgaaca gcactgaggc tgagaccatc ctggacaaca tcactcagtc tacccagtct 660 ttcaacgact tcaccagagt ggtggggggc gaggacgcca agcctggcca gttcccctgg 720 caggtcgtgc tgaacggcaa ggtggacgcc ttctgcgggg gcagcatcgt caacgagaag 780 tggatcgtga ctgccgccca ctgcgtggag actggggtga agatcactgt ggtggctggg 840 gagcacaaca tcgaggaaac cgagcacact gagcagaaga ggaacgtgat caggattatc 900 ccccaccaca actacaacgc cgccatcaac aagtacaacc acgacatcgc cctgctggag 960 ctggatgaac ccctggtgct gaacagctac gtgaccccta tctgcatcgc cgacaaggag 1020 tacactaaca tcttcctgaa gttcggcagc ggctacgtga gcggctgggg cagggtgttc 1080 cacaagggca ggagcgccct ggtgctgcag tacctgaggg tgcccctggt ggacagggcc 1140 acctgcctgc tgagcaccaa gttcaccatc tacaacaaca tgttctgcgc cggcttccac 1200 gagggcggca gggacagctg ccagggcgac agcggcggcc cccacgtgac cgaggtggag 1260 ggcaccagct tcctgaccgg catcatcagc tggggcgagg agtgcgccat gaagggcaag 1320 tacggcatct acaccaaggt gagcaggtac gtgaactgga tcaaggagaa gaccaagctg 1380 acctaa 1386 <210> 8 <211> 1389 <212> ADN <213> Secuencia Artificial <220> <223> secuencia de nucleótidos del factor IX <400> 8 atgcagcgcg tgaacatgat catggcagaa tcaccaggcc tcatcaccat ctgcctttta 60 ggatatctac tcagtgctga atgtacagtt tttcttgatc atgaaaacgc caacaaaatt 120 ctgaatcggc caaagaggta taattcaggt aaattggaag agtttgttca agggaatcta 180 gagagagaat gtatggaaga aaagtgtagt tttgaagaag cacgagaagt ttttgaaaac 240 actgaaagaa caactgaatt ttggaagcag tatgttgatg gagatcagtg tgagtccaat 300 ccatgtttaa atggcggcag ttgcaaggat gacattaatt cctatgaatg ttggtgtccc 360 tttggatttg aaggaaagaa ctgtgaatta gatgtaacat gtaacattaa gaatggcaga 420 tgcgagcagt tttgtaaaaa tagtgctgat aacaaggtgg tttgctcctg tactgaggga 480 tatcgacttg cagaaaacca gaagtcctgt gaaccagcag tgccatttcc atgtggaaga 540 gtttctgttt cacaaacttc taagctcacc cgtgctgaga ctgtttttcc tgatgtggac 600 tatgtaaatt ctactgaagc tgaaaccatt ttggataaca tcactcaaag cacccaatca 660 tttaatgact tcactcgggt tgttggtgga gaagatgcca aaccaggtca attcccttgg 720 caggttgttt tgaatggtaa agttgatgca ttctgtggag gctctatcgt taatgaaaaa 780 tggattgtaa ctgctgccca ctgtgttgaa actggtgtta aaattacagt tgtcgcaggt 840 gaacataata ttgaggagac agaacataca gagcaaaagc gaaatgtgat tcgaattatt 900 cctcaccaca actacaatgc agctattaat aagtacaacc atgacattgc ccttctggaa 960 ctggacgaac ccttagtgct aaacagctac gttacaccta tttgcattgc tgacaaggaa 1020 tacacgaaca tcttcctcaa atttggatct ggctatgtaa gtggctgggg aagagtcttc 1080 cacaaaggga gatcagcttt agttcttcag taccttagag ttccacttgt tgaccgagcc 1140 acatgtcttc gatctacaaa gttcaccatc tataacaaca tgttctgtgc tggcttccat 1200 gaaggaggta gagattcatg tcaaggagat agtgggggac cccatgttac tgaagtggaa 1260 gggaccagtt tcttaactgg aattattagc tggggtgaag agtgtgcaat gaaaggcaaa 1320 tatggaatat ataccaaggt atcccggtat gtcaactgga ttaaggaaaa aacaaagctc 1380 acttgataa 1389 <210> 9 <211> 1389 <212> ADN <213> Secuencia Artificial <220> <223> coFIX-1 <400> 9 atgcagagag tcaacatgat tatggctgag tcacctgggc tgattactat ttgcctgctg 60 ggctacctgc tgtccgccga gtgtaccgtg ttcctggacc atgagaacgc aaataagatc 120 ctgaacaggc ccaaaagata caatagtggg aagctggagg aatttgtgca gggcaacctg 180 gagagagaat gcatggagga aaagtgtagc ttcgaggaag cccgcgaggt gtttgaaaat 240 acagagcgaa ccacagagtt ctggaagcag tatgtggacg gcgatcagtg cgagagcaac 300 ccctgtctga atggcggaag ttgcaaagac gatatcaact catacgaatg ctggtgtcct 360 ttcgggtttg aaggcaaaaa ttgcgagctg gacgtgacat gtaacattaa gaatggacgg 420 tgcgagcagt tttgtaaaaa ctctgccgat aataaggtgg tgtgcagctg tactgaagga 480 tatcgcctgg ctgagaacca gaagtcctgc gaaccagcag tgcccttccc ttgtgggagg 540 gtgagcgtct cccagacttc aaaactgacc agagcagaga cagtgtttcc cgacgtggat 600 tacgtcaaca gcactgaggc cgaaaccatc ctggacaaca ttactcagtc tacccagagt 660 ttcaatgact ttactcgggt ggtcgggggc gaggatgcta aaccaggcca gttcccctgg 720 caggtggtcc tgaacggaaa ggtggatgca ttttgcggag ggtctatcgt gaatgagaaa 780 tggattgtca ccgccgctca ctgcgtggaa accggagtca agatcacagt ggtcgctggg 840 gagcacaaca ttgaggaaac agaacatact gagcagaagc ggaatgtgat ccgcatcatt 900 cctcaccata actacaatgc agccatcaac aaatacaatc atgacattgc cctgctggaa 960 ctggatgagc ctctggtgct gaacagctac gtcactccaa tctgcattgc tgacaaagag 1020 tataccaata tcttcctgaa gtttggatca gggtacgtga gcggctgggg aagagtcttc 1080 cacaagggca ggagcgccct ggtgctccag tatctgcgag tgcctctggt cgatcgagct 1140 acctgtctga ggtctaccaa gtttacaatc tacaacaaca tgttctgcgc tgggtttcac 1200 gagggaggac gagactcctg tcagggcgat tctgggggcc cacatgtgac agaggtcgaa 1260 ggcaccagct tcctgactgg catcatttcc tggggagagg aatgtgcaat gaagggaaaa 1320 tacgggatct acaccaaagt gagccgctat gtgaactgga tcaaggaaaa aaccaaactg 1380 acctaatga 1389 <210> 10 <211> 28 <212> PRT <213> Secuencia Artificial <220> <223> Péptido Señal de FIX <400> 10 Met Gln Arg Val Asn Met Ile Met Ala Glu Ser Pro Gly Leu Ile Thr 1 5 10 15 Ile Cys Leu Leu Gly Tyr Leu Leu Ser Ala Glu Cys 20 25 <210> 11 <211> 18 <212> PRT <213> Secuencia Artificial <220> <223> Propéptido de FIX <400> 11 Thr Val Phe Leu Asp His Glu Asn Ala Asn Lys Ile Leu Asn Arg Pro 1 5 10 15 Lys Arg <210> 12 <211> 415 <212> PRT <213> Secuencia Artificial <220> <223> FIX R338L <400> 12 Tyr Asn Ser Gly Lys Leu Glu Glu Phe Val Gln Gly Asn Leu Glu Arg 1 5 10 15 Glu Cys Met Glu Glu Lys Cys Ser Phe Glu Glu Ala Arg Glu Val Phe 20 25 30 Glu Asn Thr Glu Arg Thr Thr Glu Phe Trp Lys Gln Tyr Val Asp Gly 35 40 45 Asp Gln Cys Glu Ser Asn Pro Cys Leu Asn Gly Gly Ser Cys Lys Asp 50 55 60 Asp Ile Asn Ser Tyr Glu Cys Trp Cys Pro Phe Gly Phe Glu Gly Lys 65 70 75 80 Asn Cys Glu Leu Asp Val Thr Cys Asn Ile Lys Asn Gly Arg Cys Glu 85 90 95 Gln Phe Cys Lys Asn Ser Ala Asp Asn Lys Val Val Cys Ser Cys Thr 100 105 110 Glu Gly Tyr Arg Leu Ala Glu Asn Gln Lys Ser Cys Glu Pro Ala Val 115 120 125 Pro Phe Pro Cys Gly Arg Val Ser Val Ser Gln Thr Ser Lys Leu Thr 130 135 140 Arg Ala Glu Thr Val Phe Pro Asp Val Asp Tyr Val Asn Ser Thr Glu 145 150 155 160 Ala Glu Thr Ile Leu Asp Asn Ile Thr Gln Ser Thr Gln Ser Phe Asn 165 170 175 Asp Phe Thr Arg Val Val Gly Gly Glu Asp Ala Lys Pro Gly Gln Phe 180 185 190 Pro Trp Gln Val Val Leu Asn Gly Lys Val Asp Ala Phe Cys Gly Gly 195 200 205 Ser Ile Val Asn Glu Lys Trp Ile Val Thr Ala Ala His Cys Val Glu 210 215 220 Thr Gly Val Lys Ile Thr Val Val Ala Gly Glu His Asn Ile Glu Glu 225 230 235 240 Thr Glu His Thr Glu Gln Lys Arg Asn Val Ile Arg Ile Ile Pro His 245 250 255 His Asn Tyr Asn Ala Ala Ile Asn Lys Tyr Asn His Asp Ile Ala Leu 260 265 270 Leu Glu Leu Asp Glu Pro Leu Val Leu Asn Ser Tyr Val Thr Pro Ile 275 280 285 Cys Ile Ala Asp Lys Glu Tyr Thr Asn Ile Phe Leu Lys Phe Gly Ser 290 295 300 Gly Tyr Val Ser Gly Trp Gly Arg Val Phe His Lys Gly Arg Ser Ala 305 310 315 320 Leu Val Leu Gln Tyr Leu Arg Val Pro Leu Val Asp Arg Ala Thr Cys 325 330 335 Leu Leu Ser Thr Lys Phe Thr Ile Tyr Asn Asn Met Phe Cys Ala Gly 340 345 350 Phe His Glu Gly Gly Arg Asp Ser Cys Gln Gly Asp Ser Gly Gly Pro 355 360 365 His Val Thr Glu Val Glu Gly Thr Ser Phe Leu Thr Gly Ile Ile Ser 370 375 380 Trp Gly Glu Glu Cys Ala Met Lys Gly Lys Tyr Gly Ile Tyr Thr Lys 385 390 395 400 Val Ser Arg Tyr Val Asn Trp Ile Lys Glu Lys Thr Lys Leu Thr 405 410 415 <210> 13 <400> 13 000 <210> 14 <211> 323 <212> PRT <213> Secuencia Artificial <220> <223> CD47 <400> 14 Met Trp Pro Leu Val Ala Ala Leu Leu Leu Gly Ser Ala Cys Cys Gly 1 5 10 15 Ser Ala Gln Leu Leu Phe Asn Lys Thr Lys Ser Val Glu Phe Thr Phe 20 25 30 Cys Asn Asp Thr Val Val Ile Pro Cys Phe Val Thr Asn Met Glu Ala 35 40 45 Gln Asn Thr Thr Glu Val Tyr Val Lys Trp Lys Phe Lys Gly Arg Asp 50 55 60 Ile Tyr Thr Phe Asp Gly Ala Leu Asn Lys Ser Thr Val Pro Thr Asp 65 70 75 80 Phe Ser Ser Ala Lys Ile Glu Val Ser Gln Leu Leu Lys Gly Asp Ala 85 90 95 Ser Leu Lys Met Asp Lys Ser Asp Ala Val Ser His Thr Gly Asn Tyr 100 105 110 Thr Cys Glu Val Thr Glu Leu Thr Arg Glu Gly Glu Thr Ile Ile Glu 115 120 125 Leu Lys Tyr Arg Val Val Ser Trp Phe Ser Pro Asn Glu Asn Ile Leu 130 135 140 Ile Val Ile Phe Pro Ile Phe Ala Ile Leu Leu Phe Trp Gly Gln Phe 145 150 155 160 Gly Ile Lys Thr Leu Lys Tyr Arg Ser Gly Gly Met Asp Glu Lys Thr 165 170 175 Ile Ala Leu Leu Val Ala Gly Leu Val Ile Thr Val Ile Val Ile Val 180 185 190 Gly Ala Ile Leu Phe Val Pro Gly Glu Tyr Ser Leu Lys Asn Ala Thr 195 200 205 Gly Leu Gly Leu Ile Val Thr Ser Thr Gly Ile Leu Ile Leu Leu His 210 215 220 Tyr Tyr Val Phe Ser Thr Ala Ile Gly Leu Thr Ser Phe Val Ile Ala 225 230 235 240 Ile Leu Val Ile Gln Val Ile Ala Tyr Ile Leu Ala Val Val Gly Leu 245 250 255 Ser Leu Cys Ile Ala Ala Cys Ile Pro Met His Gly Pro Leu Leu Ile 260 265 270 Ser Gly Leu Ser Ile Leu Ala Leu Ala Gln Leu Leu Gly Leu Val Tyr 275 280 285 Met Lys Phe Val Ala Ser Asn Gln Lys Thr Ile Gln Pro Pro Arg Lys 290 295 300 Ala Val Glu Glu Pro Leu Asn Ala Phe Lys Glu Ser Lys Gly Met Met 305 310 315 320 Asn Asp Glu <210> 15 <211> 11 <212> PRT <213> Secuencia Artificial <220> <223> secuencia central del péptido de unión a albúmina <400> 15 Asp Ile Cys Leu Pro Arg Trp Gly Cys Leu Trp 1 5 10 <210> 16 <211> 5 <212> PRT <213> Secuencia Artificial <220> <223> conector peptídico <400> 16 Gly Gly Gly Gly Ser 1 5 <210> 17 <211> 10 <212> PRT <213> Secuencia Artificial <220> <223> conector peptídico <400> 17 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 <210> 18 <211> 32 <212> PRT <213> Secuencia Artificial <220> <223> Péptido CTP <400> 18 Asp Pro Arg Phe Gln Asp Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser 1 5 10 15 Leu Pro Ser Pro Ser Arg Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu 20 25 30 <210> 19 <211> 28 <212> PRT <213> Secuencia Artificial <220> <223> PÉPTIDO CTP <400> 19 Ser Ser Ser Ser Lys Ala Pro Pro Pro Ser Leu Pro Ser Pro Ser Arg 1 5 10 15 Leu Pro Gly Pro Ser Asp Thr Pro Ile Leu Pro Gln 20 25 <210> 20 <211> 20 <212> PRT <213> Secuencia Artificial <220> <223> Péptido PAS <400> 20 Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro 1 5 10 15 Ser Ala Pro Ala 20 <210> 21 <211> 20 <212> PRT <213> Secuencia Artificial <220> <223> Péptido PAS <400> 21 Ala Ala Pro Ala Ser Pro Ala Pro Ala Ala Pro Ser Ala Pro Ala Pro 1 5 10 15 Ala Ala Pro Ser 20 <210> 22 <211> 20 <212> PRT <213> Secuencia Artificial <220> <223> Péptido PAS <400> 22 Ala Pro Ser Ser Pro Ser Pro Ser Ala Pro Ser Ser Pro Ser Pro Ala 1 5 10 15 Ser Pro Ser Ser 20 <210> 23 <211> 19 <212> PRT <213> Secuencia Artificial <220> <223> Péptido PAS <400> 23 Ala Pro Ser Ser Pro Ser Pro Ser Ala Pro Ser Ser Pro Ser Pro Ala 1 5 10 15 Ser Pro Ser <210> 24 <211> 20 <212> PRT <213> Secuencia Artificial <220> <223> Péptido PAS <400> 24 Ser Ser Pro Ser Ala Pro Ser Pro Ser Ser Pro Ala Ser Pro Ser Pro 1 5 10 15 Ser Ser Pro Ala 20 <210> 25 <211> 24 <212> PRT <213> Secuencia Artificial <220> <223> Péptido Pas <400> 25 Ala Ala Ser Pro Ala Ala Pro Ser Ala Pro Pro Ala Ala Ala Ser Pro 1 5 10 15 Ala Ala Pro Ser Ala Pro Pro Ala 20 <210> 26 <211> 20 <212> PRT <213> Secuencia Artificial <220> <223> Péptido Pas <400> 26 Ala Ser Ala Ala Ala Pro Ala Ala Ala Ser Ala Ala Ala Ser Ala Pro 1 5 10 15 Ser Ala Ala Ala 20 <210> 27 <211> 23 <212> ADN <213> Secuencia Artificial <220> <223> Diana de miR142 <400> 27 tccataaagt aggaaacact aca 23