Listado de secuencias <110> JSC BIOCAD <120> USO DE PROTEÍNA CAS9 DE LA BACTERIA PASTEURELLA PNEUMOTROPICA <150> RU 2019136164 <151> 2019-11-11 <160> 2 <210> 1 <211> 1055 <212> PRT <213> Pasteurella pneumotropica <220> <223> Proteína homóloga a Cas9 <400> 1 Met Gln Asn Asn Pro Leu Asn Tyr Ile Leu Gly Leu Asp Leu Gly Ile 1 5 10 15 Ala Ser Ile Gly Trp Ala Val Val Glu Ile Asp Glu Glu Ser Ser Pro 20 25 30 Ile Arg Leu Ile Asp Val Gly Val Arg Thr Phe Glu Arg Ala Glu Val 35 40 45 Ala Lys Thr Gly Glu Ser Leu Ala Leu Ser Arg Arg Leu Ala Arg Ser 50 55 60 Ser Arg Arg Leu Ile Lys Arg Arg Ala Glu Arg Leu Lys Lys Ala Lys 65 70 75 80 Arg Leu Leu Lys Ala Glu Lys Ile Leu His Ser Ile Asp Glu Lys Leu 85 90 95 Pro Ile Asn Val Trp Gln Leu Arg Val Lys Gly Leu Lys Glu Lys Leu 100 105 110 Glu Arg Gln Glu Trp Ala Ala Val Leu Leu His Leu Ser Lys His Arg 115 120 125 Gly Tyr Leu Ser Gln Arg Lys Asn Glu Gly Lys Ser Asp Asn Lys Glu 130 135 140 Leu Gly Ala Leu Leu Ser Gly Ile Ala Ser Asn His Gln Met Leu Gln 145 150 155 160 Ser Ser Glu Tyr Arg Thr Pro Ala Glu Ile Ala Val Lys Lys Phe Gln 165 170 175 Val Glu Glu Gly His Ile Arg Asn Gln Arg Gly Ser Tyr Thr His Thr 180 185 190 Phe Ser Arg Leu Asp Leu Leu Ala Glu Met Glu Leu Leu Phe Gln Arg 195 200 205 Gln Ala Glu Leu Gly Asn Ser Tyr Thr Ser Thr Thr Leu Leu Glu Asn 210 215 220 Leu Thr Ala Leu Leu Met Trp Gln Lys Pro Ala Leu Ala Gly Asp Ala 225 230 235 240 Ile Leu Lys Met Leu Gly Lys Cys Thr Phe Glu Pro Ser Glu Tyr Lys 245 250 255 Ala Ala Lys Asn Ser Tyr Ser Ala Glu Arg Phe Val Trp Leu Thr Lys 260 265 270 Leu Asn Asn Leu Arg Ile Leu Glu Asn Gly Thr Glu Arg Ala Leu Asn 275 280 285 Asp Asn Glu Arg Phe Ala Leu Leu Glu Gln Pro Tyr Glu Lys Ser Lys 290 295 300 Leu Thr Tyr Ala Gln Val Arg Ala Met Leu Ala Leu Ser Asp Asn Ala 305 310 315 320 Ile Phe Lys Gly Val Arg Tyr Leu Gly Glu Asp Lys Lys Thr Val Glu 325 330 335 Ser Lys Thr Thr Leu Ile Glu Met Lys Phe Tyr His Gln Ile Arg Lys 340 345 350 Thr Leu Gly Ser Ala Glu Leu Lys Lys Glu Trp Asn Glu Leu Lys Gly 355 360 365 Asn Ser Asp Leu Leu Asp Glu Ile Gly Thr Ala Phe Ser Leu Tyr Lys 370 375 380 Thr Asp Asp Asp Ile Cys Arg Tyr Leu Glu Gly Lys Leu Pro Glu Arg 385 390 395 400 Val Leu Asn Ala Leu Leu Glu Asn Leu Asn Phe Asp Lys Phe Ile Gln 405 410 415 Leu Ser Leu Lys Ala Leu His Gln Ile Leu Pro Leu Met Leu Gln Gly 420 425 430 Gln Arg Tyr Asp Glu Ala Val Ser Ala Ile Tyr Gly Asp His Tyr Gly 435 440 445 Lys Lys Ser Thr Glu Thr Thr Arg Leu Leu Pro Thr Ile Pro Ala Asp 450 455 460 Glu Ile Arg Asn Pro Val Val Leu Arg Thr Leu Thr Gln Ala Arg Lys 465 470 475 480 Val Ile Asn Ala Val Val Arg Leu Tyr Gly Ser Pro Ala Arg Ile His 485 490 495 Ile Glu Thr Ala Arg Glu Val Gly Lys Ser Tyr Gln Asp Arg Lys Lys 500 505 510 Leu Glu Lys Gln Gln Glu Asp Asn Arg Lys Gln Arg Glu Ser Ala Val 515 520 525 Lys Lys Phe Lys Glu Met Phe Pro His Phe Val Gly Glu Pro Lys Gly 530 535 540 Lys Asp Ile Leu Lys Met Arg Leu Tyr Glu Leu Gln Gln Ala Lys Cys 545 550 555 560 Leu Tyr Ser Gly Lys Ser Leu Glu Leu His Arg Leu Leu Glu Lys Gly 565 570 575 Tyr Val Glu Val Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp 580 585 590 Ser Phe Asn Asn Lys Val Leu Val Leu Ala Asn Glu Asn Gln Asn Lys 595 600 605 Gly Asn Leu Thr Pro Tyr Glu Trp Leu Asp Gly Lys Asn Asn Ser Glu 610 615 620 Arg Trp Gln His Phe Val Val Arg Val Gln Thr Ser Gly Phe Ser Tyr 625 630 635 640 Ala Lys Lys Gln Arg Ile Leu Asn His Lys Leu Asp Glu Lys Gly Phe 645 650 655 Ile Glu Arg Asn Leu Asn Asp Thr Arg Tyr Val Ala Arg Phe Leu Cys 660 665 670 Asn Phe Ile Ala Asp Asn Met Leu Leu Val Gly Lys Gly Lys Arg Asn 675 680 685 Val Phe Ala Ser Asn Gly Gln Ile Thr Ala Leu Leu Arg His Arg Trp 690 695 700 Gly Leu Gln Lys Val Arg Glu Gln Asn Asp Arg His His Ala Leu Asp 705 710 715 720 Ala Val Val Val Ala Cys Ser Thr Val Ala Met Gln Gln Lys Ile Thr 725 730 735 Arg Phe Val Arg Tyr Asn Glu Gly Asn Val Phe Ser Gly Glu Arg Ile 740 745 750 Asp Arg Glu Thr Gly Glu Ile Ile Pro Leu His Phe Pro Ser Pro Trp 755 760 765 Ala Phe Phe Lys Glu Asn Val Glu Ile Arg Ile Phe Ser Glu Asn Pro 770 775 780 Lys Leu Glu Leu Glu Asn Arg Leu Pro Asp Tyr Pro Gln Tyr Asn His 785 790 795 800 Glu Trp Val Gln Pro Leu Phe Val Ser Arg Met Pro Thr Arg Lys Met 805 810 815 Thr Gly Gln Gly His Met Glu Thr Val Lys Ser Ala Lys Arg Leu Asn 820 825 830 Glu Gly Leu Ser Val Leu Lys Val Pro Leu Thr Gln Leu Lys Leu Ser 835 840 845 Asp Leu Glu Arg Met Val Asn Arg Asp Arg Glu Ile Ala Leu Tyr Glu 850 855 860 Ser Leu Lys Ala Arg Leu Glu Gln Phe Gly Asn Asp Pro Ala Lys Ala 865 870 875 880 Phe Ala Glu Pro Phe Tyr Lys Lys Gly Gly Ala Leu Val Lys Ala Val 885 890 895 Arg Leu Glu Gln Thr Gln Lys Ser Gly Val Leu Val Arg Asp Gly Asn 900 905 910 Gly Val Ala Asp Asn Ala Ser Met Val Arg Val Asp Val Phe Thr Lys 915 920 925 Gly Gly Lys Tyr Phe Leu Val Pro Ile Tyr Thr Trp Gln Val Ala Lys 930 935 940 Gly Ile Leu Pro Asn Arg Ala Ala Thr Gln Gly Lys Asp Glu Asn Asp 945 950 955 960 Trp Asp Ile Met Asp Glu Met Ala Thr Phe Gln Phe Ser Leu Cys Gln 965 970 975 Asn Asp Leu Ile Lys Leu Val Thr Lys Lys Lys Thr Ile Phe Gly Tyr 980 985 990 Phe Asn Gly Leu Asn Arg Ala Thr Ser Asn Ile Asn Ile Lys Glu His 995 1000 1005 Asp Leu Asp Lys Ser Lys Gly Lys Leu Gly Ile Tyr Leu Glu Val Gly 1010 1015 1020 Val Lys Leu Ala Ile Ser Leu Glu Lys Tyr Gln Val Asp Glu Leu Gly 1025 1030 1035 1040 Lys Asn Ile Arg Pro Cys Arg Pro Thr Lys Arg Gln His Val Arg 1045 1050 1055 <210> 2 <211> 3168 <212> ADN <213> Pasteurella pneumotropica <220> <223> Proteína homóloga a Cas9 <400> 2 atgcaaaata atccattaaa ttacatttta gggttagatt taggcattgc ttctattggt 60 tgggcggttg tggaaattga tgaggagagt tcacctattc gcttaattga tgtgggcgtc 120 cgtacatttg aacgggctga agtcgctaaa accggcgaaa gtttagcatt gtctcgtcgt 180 ttagctcgtt catcacggcg attaattaaa cgccgagcag agcgattaaa aaaagcaaaa 240 cgtttattaa aagcagaaaa gattttacat tctattgatg aaaaattacc cattaatgtt 300 tggcagcttc gagtaaaagg attgaaggaa aaactcgaac gtcaggagtg ggcagcggtt 360 ttattacatt tgtcaaagca tcgtggctat ttatcacaac gtaaaaatga gggtaaaagt 420 gataataaag agctgggggc attactttca ggtatcgcaa gtaaccacca aatgttgcaa 480 tcctccgaat atcgtacccc tgcagaaatt gcagtcaaaa aatttcaagt agaagaagga 540 catattcgta atcaacgtgg atcttatacc cataccttta gccgtttgga tttgttggca 600 gaaatggaat tattatttca acgccaagct gagttaggca attcttacac gtccaccaca 660 ttattagaaa atttgacggc gttactaatg tggcaaaagc cagctcttgc gggtgatgcg 720 attttaaaaa tgttgggcaa gtgtaccttc gaacccagcg aatataaagc cgcaaaaaat 780 agttattctg ctgaacgttt tgtgtggtta accaagctga ataatttacg cattttagaa 840 aatggcacgg aaagagcttt aaatgacaat gaacgttttg ctttgcttga gcaaccgtat 900 gagaaatcaa aattaactta tgctcaagtg agagcaatgc ttgcgttatc tgataatgct 960 attttcaaag gggttcgtta tttaggcgaa gataaaaaaa cagtagagag caaaactacg 1020 ttgatagaaa tgaagtttta tcatcaaatc cgcaaaacat taggcagtgc agaattaaaa 1080 aaggaatgga atgagttaaa aggcaattcc gatttattag atgagattgg cacggcattt 1140 tcgttgtata aaacggatga tgatatttgc cgttatttag agggaaaact accagaaagg 1200 gtattaaatg cgttattgga aaatttaaat ttcgataaat ttattcaact ttcacttaaa 1260 gccttacacc aaattttacc attgatgctg caagggcaac gttatgatga ggcggtttct 1320 gcgatttatg gtgatcatta tggtaaaaaa tcgacagaaa caacccgctt gttgccgact 1380 attcctgccg atgaaatccg aaatcctgtg gtattacgca ccctgaccca agcccgtaaa 1440 gtgatcaatg cggtggtgcg gttatatggt tcgcctgccc gtattcatat tgaaacagcg 1500 agagaagtcg gcaaatctta ccaagatcgt aaaaaacttg aaaaacagca agaagataat 1560 cgtaagcaac gtgaaagtgc ggtcaaaaaa tttaaagaaa tgtttccgca ttttgtgggg 1620 gagccgaaag gtaaagatat tttaaaaatg cgattgtatg agttacaaca agcgaaatgt 1680 ttatattctg gaaaatcttt agaacttcat cgtttgcttg agaaggggta tgtagaagtg 1740 gatcacgctt tgccattttc tcgcacgtgg gatgatagct ttaataataa agtactggtg 1800 cttgccaacg agaaccaaaa taaaggcaat ttaacgcctt atgaatggtt agatggtaaa 1860 aataacagtg agcgttggca acattttgtt gtacgagtac aaaccagcgg tttctcttat 1920 gctaaaaaac aacgcatttt gaaccataaa ttggatgaaa aagggtttat cgaacgtaat 1980 ttaaacgata ctcgctatgt agctcgtttc ttatgtaact ttattgccga taatatgttg 2040 ttggttggta aaggcaagcg aaacgtgttt gcttcaaacg ggcaaatcac ggcgttattg 2100 cggcatcgtt ggggcttaca aaaagtgcgt gaacagaatg atcgccacca cgcactggac 2160 gcggttgtgg tggcttgctc tactgtggca atgcaacaaa aaatcactcg atttgtgaga 2220 tataacgaag gaaatgtctt tagcggtgaa cgtatcgatc gtgaaactgg cgagattatt 2280 ccattacatt ttccaagccc ttgggctttt ttcaaagaga atgtggaaat tcgcattttt 2340 agtgaaaatc caaaattgga attagaaaat cgcctgcctg attatccgca atataatcac 2400 gaatgggtgc aaccattgtt tgtttcgaga atgccaaccc gaaaaatgac agggcaaggg 2460 catatggaaa cggtaaaatc cgcaaaacga ttaaatgaag gtttaagtgt gttaaaagtc 2520 cctttaacac aacttaaatt gagtgattta gaacgaatgg ttaatcgtga tcgtgaaatt 2580 gcattgtatg aatccttaaa agcacgttta gagcaatttg gtaacgaccc agccaaagcc 2640 tttgccgaac cattctataa aaagggtggg gcattagtca aagcagtccg attggaacaa 2700 acacaaaaat cgggggtatt agtacgtgat ggtaacggtg ttgcggataa tgcttcaatg 2760 gtacgggttg atgtttttac taaaggtgga aaatatttct tagtgccgat ttatacttgg 2820 caggtagcga aagggatttt accgaatagg gctgcgacac aaggtaaaga tgaaaatgat 2880 tgggatatta tggatgaaat ggctactttc caattttctc tatgtcaaaa tgatctaatt 2940 aaattagtta ccaaaaagaa aacaatcttt ggatatttta atggattaaa tagagctact 3000 agcaatataa atattaaaga gcatgatcta gataagtcta aagggaaatt aggtatttac 3060 ttagaagttg gtgtaaaact agctatttcc cttgaaaagt accaagtcga cgaactcggc 3120 aaaaatatcc gtccttgtcg tccgactaaa cgacagcacg tgcgttaa 3168