SEQUENCE LISTING <110> Washington University BATEMAN, Randall HOLTZMAN, David BUDELIER, Melissa JIANG, Hong <120> METHODS FOR DETECTING NEUROFILAMENT LIGHT CHAIN IN PLASMA AND CEREBROSPINAL FLUID <130> 047563-716468 <150> 63/197,826 <151> 2021-06-21 <150> 63/183,417 <151> 2021-05-03 <150> 63/147,833 <151> 2021-02-10 <160> 25 <170> PatentIn version 3.5 <210> 1 <211> 543 <212> PRT <213> Homo sapiens <400> 1 Met Ser Ser Phe Ser Tyr Glu Pro Tyr Tyr Ser Thr Ser Tyr Lys Arg 1 5 10 15 Arg Tyr Val Glu Thr Pro Arg Val His Ile Ser Ser Val Arg Ser Gly 20 25 30 Tyr Ser Thr Ala Arg Ser Ala Tyr Ser Ser Tyr Ser Ala Pro Val Ser 35 40 45 Ser Ser Leu Ser Val Arg Arg Ser Tyr Ser Ser Ser Ser Gly Ser Leu 50 55 60 Met Pro Ser Leu Glu Asn Leu Asp Leu Ser Gln Val Ala Ala Ile Ser 65 70 75 80 Asn Asp Leu Lys Ser Ile Arg Thr Gln Glu Lys Ala Gln Leu Gln Asp 85 90 95 Leu Asn Asp Arg Phe Ala Ser Phe Ile Glu Arg Val His Glu Leu Glu 100 105 110 Gln Gln Asn Lys Val Leu Glu Ala Glu Leu Leu Val Leu Arg Gln Lys 115 120 125 His Ser Glu Pro Ser Arg Phe Arg Ala Leu Tyr Glu Gln Glu Ile Arg 130 135 140 Asp Leu Arg Leu Ala Ala Glu Asp Ala Thr Asn Glu Lys Gln Ala Leu 145 150 155 160 Gln Gly Glu Arg Glu Gly Leu Glu Glu Thr Leu Arg Asn Leu Gln Ala 165 170 175 Arg Tyr Glu Glu Glu Val Leu Ser Arg Glu Asp Ala Glu Gly Arg Leu 180 185 190 Met Glu Ala Arg Lys Gly Ala Asp Glu Ala Ala Leu Ala Arg Ala Glu 195 200 205 Leu Glu Lys Arg Ile Asp Ser Leu Met Asp Glu Ile Ser Phe Leu Lys 210 215 220 Lys Val His Glu Glu Glu Ile Ala Glu Leu Gln Ala Gln Ile Gln Tyr 225 230 235 240 Ala Gln Ile Ser Val Glu Met Asp Val Thr Lys Pro Asp Leu Ser Ala 245 250 255 Ala Leu Lys Asp Ile Arg Ala Gln Tyr Glu Lys Leu Ala Ala Lys Asn 260 265 270 Met Gln Asn Ala Glu Glu Trp Phe Lys Ser Arg Phe Thr Val Leu Thr 275 280 285 Glu Ser Ala Ala Lys Asn Thr Asp Ala Val Arg Ala Ala Lys Asp Glu 290 295 300 Val Ser Glu Ser Arg Arg Leu Leu Lys Ala Lys Thr Leu Glu Ile Glu 305 310 315 320 Ala Cys Arg Gly Met Asn Glu Ala Leu Glu Lys Gln Leu Gln Glu Leu 325 330 335 Glu Asp Lys Gln Asn Ala Asp Ile Ser Ala Met Gln Asp Thr Ile Asn 340 345 350 Lys Leu Glu Asn Glu Leu Arg Thr Thr Lys Ser Glu Met Ala Arg Tyr 355 360 365 Leu Lys Glu Tyr Gln Asp Leu Leu Asn Val Lys Met Ala Leu Asp Ile 370 375 380 Glu Ile Ala Ala Tyr Arg Lys Leu Leu Glu Gly Glu Glu Thr Arg Leu 385 390 395 400 Ser Phe Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr Ser Gln Ser Ser 405 410 415 Gln Val Phe Gly Arg Ser Ala Tyr Gly Gly Leu Gln Thr Ser Ser Tyr 420 425 430 Leu Met Ser Thr Arg Ser Phe Pro Ser Tyr Tyr Thr Ser His Val Gln 435 440 445 Glu Glu Gln Ile Glu Val Glu Glu Thr Ile Glu Ala Ala Lys Ala Glu 450 455 460 Glu Ala Lys Asp Glu Pro Pro Ser Glu Gly Glu Ala Glu Glu Glu Glu 465 470 475 480 Lys Asp Lys Glu Glu Ala Glu Glu Glu Glu Ala Ala Glu Glu Glu Glu 485 490 495 Ala Ala Lys Glu Glu Ser Glu Glu Ala Lys Glu Glu Glu Glu Gly Gly 500 505 510 Glu Gly Glu Glu Gly Glu Glu Thr Lys Glu Ala Glu Glu Glu Glu Lys 515 520 525 Lys Val Glu Gly Ala Gly Glu Glu Gln Ala Ala Lys Lys Lys Asp 530 535 540 <210> 2 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 2 Tyr Val Glu Thr Pro Arg 1 5 <210> 3 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 3 Val His Ile Ser Ser Val Arg 1 5 <210> 4 <211> 17 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 4 Ser Ala Tyr Ser Ser Tyr Ser Ala Pro Val Ser Ser Ser Leu Ser Val 1 5 10 15 Arg <210> 5 <211> 29 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 5 Ser Tyr Ser Ser Ser Ser Gly Ser Leu Met Pro Ser Leu Glu Asn Leu 1 5 10 15 Asp Leu Ser Gln Val Ala Ala Ile Ser Asn Asp Leu Lys 20 25 <210> 6 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 6 Ala Gln Leu Gln Asp Leu Asn Asp Arg 1 5 <210> 7 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 7 Phe Ala Ser Phe Ile Glu Arg 1 5 <210> 8 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 8 Val His Glu Leu Glu Gln Gln Asn Lys 1 5 <210> 9 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 9 Val Leu Glu Ala Glu Leu Leu Val Leu Arg 1 5 10 <210> 10 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 10 Ala Leu Tyr Glu Gln Glu Ile Arg 1 5 <210> 11 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 11 Leu Ala Ala Glu Asp Ala Thr Asn Glu Lys 1 5 10 <210> 12 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 12 Gln Ala Leu Gln Gly Glu Arg 1 5 <210> 13 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 13 Glu Gly Leu Glu Glu Thr Leu Arg 1 5 <210> 14 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 14 Tyr Glu Glu Glu Val Leu Ser Arg 1 5 <210> 15 <211> 5 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 15 Leu Met Glu Ala Arg 1 5 <210> 16 <211> 9 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 16 Gly Ala Asp Glu Ala Ala Leu Ala Arg 1 5 <210> 17 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 17 Ile Asp Ser Leu Met Asp Glu Ile Ser Phe Leu Lys 1 5 10 <210> 18 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 18 Phe Thr Val Leu Thr Glu Ser Ala Ala Lys 1 5 10 <210> 19 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 19 Gly Met Asn Glu Ala Leu Glu Lys 1 5 <210> 20 <211> 8 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 20 Gln Leu Gln Glu Leu Glu Asp Lys 1 5 <210> 21 <211> 6 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 21 Leu Glu Asn Glu Leu Arg 1 5 <210> 22 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 22 Leu Ser Phe Thr Ser Val Gly Ser Ile Thr Ser Gly Tyr Ser Gln Ser 1 5 10 15 Ser Gln Val Phe Gly Arg 20 <210> 23 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 23 Ser Ala Tyr Gly Gly Leu Gln Thr Ser Ser Tyr Leu Met Ser Thr Arg 1 5 10 15 <210> 24 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 24 Ser Phe Pro Ser Tyr Tyr Thr Ser His Val Gln Glu Glu Gln Ile Glu 1 5 10 15 Val Glu Glu Thr Ile Glu Ala Ala Lys 20 25 <210> 25 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Synthesized <400> 25 Val Glu Gly Ala Gly Glu Glu Gln Ala Ala Lys 1 5 10