LISTADO DE SECUENCIAS <110> GIVAUDAN SA <120> CÉLULAS RECOMBINANTES PRODUCTORAS DE ÁCIDO HIALURÓNICO <130> PR92297/MGG <150> EP21306488.4 <151> 2021-10-26 <150> EP21166743.1 <151> 2021-04-01 <160> 108 <170> BiSSAP 1.3.6 <210> 1 <211> 1707 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <400> 1 atgggtaaga acatcattat tatggtttct tggtacacta ttattacatc caatctcatc 60 gcagtgggtg gcgcctcact catactagcc ccagctatta cgggctatgt ccttcactgg 120 aacattgccc tttcaacaat ttggggagtg tcggcctacg gaattttcgt gtttggtttc 180 tttcttgccc aggtattatt tagtgaactc aaccggaaaa ggctccggaa gtggatttcc 240 ctccgaccca aagggtggaa tgatgttagg ttggctgtaa ttattgccgg ctaccgtgag 300 gacccgtata tgttccaaaa gtgtcttgaa agtgtgcgtg actcagacta tgggaatgta 360 gctagactaa tatgcgttat tgacggcgat gaagacgacg acatgaggat ggctgcagtg 420 tacaaggcta tctataacga caacatcaag aaacctgagt ttgtcctctg tgagagtgac 480 gataaggagg gtgagagaat agatagcgat ttcagccgtg atatctgcgt gctgcaaccg 540 catcgcggaa agcgtgaatg tttgtacaca gggttccaat tggcaaagat ggacccctca 600 gttaatgccg tcgtcctaat cgacagtgac actgtgttag aaaaggacgc gattctcgaa 660 gtagtatacc cgctggcatg cgatccagaa atacaggctg tagcaggcga atgcaaaata 720 tggaatactg acacactgtt gagtttgctg gtagcctggc gatattacag cgcattttgc 780 gtagagcgta gcgcccaatc attcttcagg acagtacaat gcgtcggagg acctctcggc 840 gcctacaaga ttgatataat taaggaaatc aaggacccat ggatcagcca acgtttcctt 900 ggccaaaagt gcacatacgg cgacgataga cgactcacta atgaaatact aatgaggggt 960 aagaaagtag ttttcacccc attcgctgtt ggctggtccg acagcccgac gaacgtcttc 1020 cgttacattg tacagcaaac acggtggtcc aagtcgtggt gtagggagat atggtataca 1080 ctgtttgcag catggaagca tggactttcg ggcatttggc tcgcattcga gtgcttatac 1140 cagattactt atttcttcct ggtgatttac ctattctccc gtttggctgt tgaggctgac 1200 ccacgggcgc aaacggccac cgtcattgtt tcgaccacag ttgcgcttat taagtgcggt 1260 tactttagct tcagagctaa agacattagg gccttctatt tcgtcctcta cacgttcgtt 1320 tacttcttct gcatgattcc ggcacgaata actgcgatga tgaccctgtg ggacataggt 1380 tggggaacca ggggaggaaa tgagaagcct tccgtaggca ccagagttgc tttgtgggcc 1440 aagcaatatt tgattgctta catgtggtgg gccgctgtcg tcggtgctgg cgtgtactcc 1500 atcgttcata attggatgtt tgactggaat tcactttcct acaggttcgc actggtaggc 1560 atctgttcgt atatagtttt cattgtaata gtgctggtag tctactttac aggaaagatc 1620 acgacctgga acttcacgaa gctacagaaa gaattgatcg aggaccgcgt actgtacgac 1680 gcaacgacca atgcccagtc ggtataa 1707 <210> 2 <211> 1707 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <400> 2 atgggcaaga atattattat catggtcagt tggtatacca ttattacatc aaacctaatt 60 gctgtcggtg gagcctcact aatcttagct cccgcgatta cgggttatgt gcttcattgg 120 aatattgctt tatccactat ttggggtgtt agcgcttacg gcatctttgt gttcggtttc 180 ttcttggctc aggtgttatt ctctgagcta aacagaaaga gactcagaaa gtggatttcg 240 ctgcggccga agggatggaa cgatgttcgg ttagcggtga tcattgccgg ttatcgtgaa 300 gatccttata tgtttcagaa gtgtttggaa tctgttcgag actcagacta cggtaacgtt 360 gctaggctga tctgcgtaat cgatggagac gaagatgacg atatgagaat ggccgcggtt 420 tataaagcca tctataacga taacattaag aagccagagt ttgttttatg cgaatcggat 480 gacaaggagg gtgagaggat tgattctgac ttctcgcgtg acatctgtgt cctgcagccc 540 cacaggggaa agcgagagtg cctctataca ggattccaat tagctaaaat ggacccaagc 600 gttaatgctg tcgtccttat cgatagcgac acggtgttgg aaaaggacgc aatattggaa 660 gtagtctatc cactcgcttg tgatccagag atccaggccg tggctggcga gtgcaagatt 720 tggaatacag atacgcttct gtcattactg gtggcgtggc gttattattc agcattctgt 780 gtggagagga gcgcacaatc gttctttcgg actgtacaat gcgtaggcgg tccactagga 840 gcatataaaa ttgacataat caaagagata aaagatcctt ggatttcgca gagattcctc 900 ggtcaaaagt gtacctatgg ggacgacagg cggttgacta acgagattct aatgagaggt 960 aagaaggtgg tgttcactcc ttttgcggta ggttggagcg attcgcccac aaatgtattc 1020 agatacattg ttcaacaaac aaggtggtcg aaatcgtggt gccgagaaat atggtacacg 1080 ttgttcgccg catggaaaca cggcttgtcg ggtatatggc tcgctttcga gtgcctttat 1140 cagatcactt acttcttcct ggttatttac ttgttttcaa gactggcagt ggaagctgat 1200 ccaagggctc agactgctac tgtcatcgtt tcaacaacag tggcgctaat aaagtgtggt 1260 tacttcagct ttcgcgcgaa ggacataaga gcgttttact ttgttcttta tacctttgta 1320 tatttcttct gtatgatccc ggcccggata acagctatga tgacattatg ggacattggt 1380 tggggcacca ggggtgggaa tgagaagccg tccgtaggaa cgagagtagc gctgtgggcc 1440 aagcagtatc ttatcgcata catgtggtgg gcagctgtcg tcggggcagg tgtatacagc 1500 atagtacata actggatgtt tgactggaac tcactctcat atcgattcgc attggtcggg 1560 atctgctctt acatcgtgtt tattgttata gtcttagtag tatatttcac tggaaagata 1620 acaacatgga atttcactaa gcttcaaaag gaattgattg aagacagggt gctctacgat 1680 gcgacaacta atgcacaaag cgtataa 1707 <210> 3 <211> 2919 <212> DNA <213> Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <400> 3 atgaacaccc tttcccaggc aattaaggct tataatagta atgattatca actagccctc 60 aagctgttcg aaaagtcggc agaaatctac ggcagaaaga tagttgagtt ccaaattacg 120 aagtgcaagg agaaactttc tgcgcaccca tccgttaaca gtgcgcatct ctcggttaac 180 aaagaagaga aggtgaatgt ttgcgacagt ccattagaca tagccactca attattgtta 240 tcgaatgtga aaaaactcgt ccttagtgat agcgagaaaa atacactaaa gaataaatgg 300 aagttgttga ctgaaaagaa gtctgagaac gcagaggtcc gggccgtagc tttagttccc 360 aaggactttc cgaaagattt agtgcttgcg ccgttaccag accatgtgaa cgattttacg 420 tggtataaga agcggaagaa acgcctagga atcaaaccag aacaccaaca cgttggcctc 480 tccattattg tgacaacgtt caaccgtcct gccatcctca gcatcaccct ggcttgcctc 540 gtcaatcaaa agacccatta tccttttgag gtgatcgtga ccgacgatgg ttctcaagag 600 gatttatcgc ctataatccg tcaatatgag aacaagctag acatccgtta tgttcggcaa 660 aaggacaacg gttttcaggc atcggccgca cgtaatatgg gcctacgtct agccaaatat 720 gacttcattg ggctccttga ttgtgatatg gctcccaacc ctttgtgggt acactcatac 780 gttgcggaac tattagagga cgatgatttg accataatag gccccaggaa gtacattgac 840 acgcaacaca tagatcccaa ggatttcctt aataacgcct ctctgttaga gtcgttgcca 900 gaggttaaga ccaataattc cgtcgcggct aagggcgagg ggaccgtatc tttagactgg 960 cgtttggaac aatttgagaa gaccgagaac ttgaggctat ccgatagccc tttccgattc 1020 ttcgcagctg ggaatgtggc tttcgccaag aagtggctta acaagtcagg attcttcgac 1080 gaggaattca atcactgggg aggtgaagat gtagagttcg gttatcgtct gtttcggtac 1140 ggttcgttct tcaaaactat agacggcatc atggcctatc atcaggaacc gccaggtaaa 1200 gaaaacgaaa ctgacagaga agcgggcaag aacattaccc tcgatataat gagggagaag 1260 gtgccttaca tctaccgtaa actcctgcct atagaagaca gtcatatcaa ccgagtacca 1320 ttggtatcaa tttacatccc ggcctacaac tgtgctaact atattcaaag atgtgttgat 1380 tcagctttga atcaaacggt agttgatctc gaagtgtgca tttgcaacga cggtagtact 1440 gacaacacgc tggaagttat taacaagctg tatggtaata atccgcgtgt gcgtataatg 1500 tctaaaccca atggcggcat tgcgagtgca tccaacgcag cggtcagctt cgcaaagggt 1560 tattacatag gacagttgga cagcgacgat tacttagaac ccgacgcagt ggagttatgt 1620 ctcaaggaat ttcttaagga taagaccctt gcgtgcgttt acaccactaa tcgtaacgtc 1680 aacccagatg gctctttaat agccaatggc tataactggc cagagttcag tcgtgagaag 1740 ttgactacgg ccatgattgc tcatcacttc cggatgttta ccattcgtgc ttggcatctg 1800 acggatgggt tcaatgagaa gattgagaac gctgttgact acgacatgtt tctcaagctc 1860 agtgaagttg gtaaatttaa gcatctgaac aaaatatgtt ataatcgggt gttacacggc 1920 gataacacct caatcaagaa gcttggcata caaaagaaga atcatttcgt agttgtcaat 1980 cagtctctaa accgccaagg tataacttat tataactacg atgaatttga tgatctcgat 2040 gagagtcgga aatacatttt caacaagact gcagagtatc aagaagagat agatattctt 2100 aaagatatta agatcattca gaacaaagac gccaaaatag ctgtctccat tttctatccg 2160 aacactctaa acgggttggt gaagaagcta aataatatta tagagtataa taagaatatc 2220 ttcgttattg tacttcatgt cgataagaat cacttaaccc cagacatcaa gaaggagata 2280 ttagccttct accataagca tcaggtgaac atcttattga acaatgacat ctcctactat 2340 acatcaaatc gtctgattaa gacagaagcc catttgagta acattaacaa gctaagtcaa 2400 ttaaatctta actgcgaata cattatattc gacaatcacg attccttatt tgtgaagaac 2460 gattcctatg catacatgaa gaagtatgat gttggaatga acttctctgc attgactcat 2520 gattggattg aaaagataaa cgctcacccg ccatttaaga agctgatcaa aacttacttc 2580 aatgacaatg atcttaagtc gatgaatgta aagggtgcct cccagggaat gtttatgaca 2640 tacgcattag cccacgagtt attgacgatc atcaaggagg tgataacctc ttgtcaatcc 2700 attgactccg tccccgaata caacacagaa gatatttggt ttcagtttgc acttttaatt 2760 ctggaaaaga agaccggcca cgtattcaac aagacaagca ctctcacgta tatgccatgg 2820 gaacgtaaac tgcagtggac gaatgaacaa atagagtccg caaagagggg cgaaaacatt 2880 ccggtaaaca agttcatcat taacagcatt accctttaa 2919 <210> 4 <211> 2112 <212> DNA <213> Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <400> 4 atgaacaccc tttcccaggc aattaaggct tataatagta atgattatca actagccctc 60 aagctgttcg aaaagtcggc agaaatctac ggcagaaaga tagttgagtt ccaaattacg 120 aagtgcaagg agaaactttc tgcgcaccca tccgttaaca gtgcgcatct ctcggttaac 180 aaagaagaga aggtgaatgt ttgcgacagt ccattagaca tagccactca attattgtta 240 tcgaatgtga aaaaactcgt ccttagtgat agcgagaaaa atacactaaa gaataaatgg 300 aagttgttga ctgaaaagaa gtctgagaac gcagaggtcc gggccgtagc tttagttccc 360 aaggactttc cgaaagattt agtgcttgcg ccgttaccag accatgtgaa cgattttacg 420 tggtataaga agcggaagaa acgcctagga atcaaaccag aacaccaaca cgttggcctc 480 tccattattg tgacaacgtt caaccgtcct gccatcctca gcatcaccct ggcttgcctc 540 gtcaatcaaa agacccatta tccttttgag gtgatcgtga ccgacgatgg ttctcaagag 600 gatttatcgc ctataatccg tcaatatgag aacaagctag acatccgtta tgttcggcaa 660 aaggacaacg gttttcaggc atcggccgca cgtaatatgg gcctacgtct agccaaatat 720 gacttcattg ggctccttga ttgtgatatg gctcccaacc ctttgtgggt acactcatac 780 gttgcggaac tattagagga cgatgatttg accataatag gccccaggaa gtacattgac 840 acgcaacaca tagatcccaa ggatttcctt aataacgcct ctctgttaga gtcgttgcca 900 gaggttaaga ccaataattc cgtcgcggct aagggcgagg ggaccgtatc tttagactgg 960 cgtttggaac aatttgagaa gaccgagaac ttgaggctat ccgatagccc tttccgattc 1020 ttcgcagctg ggaatgtggc tttcgccaag aagtggctta acaagtcagg attcttcgac 1080 gaggaattca atcactgggg aggtgaagat gtagagttcg gttatcgtct gtttcggtac 1140 ggttcgttct tcaaaactat agacggcatc atggcctatc atcaggaacc gccaggtaaa 1200 gaaaacgaaa ctgacagaga agcgggcaag aacattaccc tcgatataat gagggagaag 1260 gtgccttaca tctaccgtaa actcctgcct atagaagaca gtcatatcaa ccgagtacca 1320 ttggtatcaa tttacatccc ggcctacaac tgtgctaact atattcaaag atgtgttgat 1380 tcagctttga atcaaacggt agttgatctc gaagtgtgca tttgcaacga cggtagtact 1440 gacaacacgc tggaagttat taacaagctg tatggtaata atccgcgtgt gcgtataatg 1500 tctaaaccca atggcggcat tgcgagtgca tccaacgcag cggtcagctt cgcaaagggt 1560 tattacatag gacagttgga cagcgacgat tacttagaac ccgacgcagt ggagttatgt 1620 ctcaaggaat ttcttaagga taagaccctt gcgtgcgttt acaccactaa tcgtaacgtc 1680 aacccagatg gctctttaat agccaatggc tataactggc cagagttcag tcgtgagaag 1740 ttgactacgg ccatgattgc tcatcacttc cggatgttta ccattcgtgc ttggcatctg 1800 acggatgggt tcaatgagaa gattgagaac gctgttgact acgacatgtt tctcaagctc 1860 agtgaagttg gtaaatttaa gcatctgaac aaaatatgtt ataatcgggt gttacacggc 1920 gataacacct caatcaagaa gcttggcata caaaagaaga atcatttcgt agttgtcaat 1980 cagtctctaa accgccaagg tataacttat tataactacg atgaatttga tgatctcgat 2040 gagagtcgga aatacatttt caacaagact gcagagtatc aagaagagat agatattctt 2100 aaagatattt aa 2112 <210> 5 <211> 2322 <212> DNA <213> Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <400> 5 atgaacaccc tttcccaggc aattaaggct tataatagta atgattatca actagccctc 60 aagctgttcg aaaagtcggc agaaatctac ggcagaaaga tagttgagtt ccaaattacg 120 aagtgcaagg agaaactttc tgcgcaccca tccgttaaca gtgcgcatct ctcggttaac 180 aaagaagaga aggtgaatgt ttgcgacagt ccattagaca tagccactca attattgtta 240 tcgaatgtga aaaaactcgt ccttagtgat agcgagaaaa atacactaaa gaataaatgg 300 aagttgttga ctgaaaagaa gtctgagaac gcagaggtcc gggccgtagc tttagttccc 360 aaggactttc cgaaagattt agtgcttgcg ccgttaccag accatgtgaa cgattttacg 420 tggtataaga agcggaagaa acgcctagga atcaaaccag aacaccaaca cgttggcctc 480 tccattattg tgacaacgtt caaccgtcct gccatcctca gcatcaccct ggcttgcctc 540 gtcaatcaaa agacccatta tccttttgag gtgatcgtga ccgacgatgg ttctcaagag 600 gatttatcgc ctataatccg tcaatatgag aacaagctag acatccgtta tgttcggcaa 660 aaggacaacg gttttcaggc atcggccgca cgtaatatgg gcctacgtct agccaaatat 720 gacttcattg ggctccttga ttgtgatatg gctcccaacc ctttgtgggt acactcatac 780 gttgcggaac tattagagga cgatgatttg accataatag gccccaggaa gtacattgac 840 acgcaacaca tagatcccaa ggatttcctt aataacgcct ctctgttaga gtcgttgcca 900 gaggttaaga ccaataattc cgtcgcggct aagggcgagg ggaccgtatc tttagactgg 960 cgtttggaac aatttgagaa gaccgagaac ttgaggctat ccgatagccc tttccgattc 1020 ttcgcagctg ggaatgtggc tttcgccaag aagtggctta acaagtcagg attcttcgac 1080 gaggaattca atcactgggg aggtgaagat gtagagttcg gttatcgtct gtttcggtac 1140 ggttcgttct tcaaaactat agacggcatc atggcctatc atcaggaacc gccaggtaaa 1200 gaaaacgaaa ctgacagaga agcgggcaag aacattaccc tcgatataat gagggagaag 1260 gtgccttaca tctaccgtaa actcctgcct atagaagaca gtcatatcaa ccgagtacca 1320 ttggtatcaa tttacatccc ggcctacaac tgtgctaact atattcaaag atgtgttgat 1380 tcagctttga atcaaacggt agttgatctc gaagtgtgca tttgcaacga cggtagtact 1440 gacaacacgc tggaagttat taacaagctg tatggtaata atccgcgtgt gcgtataatg 1500 tctaaaccca atggcggcat tgcgagtgca tccaacgcag cggtcagctt cgcaaagggt 1560 tattacatag gacagttgga cagcgacgat tacttagaac ccgacgcagt ggagttatgt 1620 ctcaaggaat ttcttaagga taagaccctt gcgtgcgttt acaccactaa tcgtaacgtc 1680 aacccagatg gctctttaat agccaatggc tataactggc cagagttcag tcgtgagaag 1740 ttgactacgg ccatgattgc tcatcacttc cggatgttta ccattcgtgc ttggcatctg 1800 acggatgggt tcaatgagaa gattgagaac gctgttgact acgacatgtt tctcaagctc 1860 agtgaagttg gtaaatttaa gcatctgaac aaaatatgtt ataatcgggt gttacacggc 1920 gataacacct caatcaagaa gcttggcata caaaagaaga atcatttcgt agttgtcaat 1980 cagtctctaa accgccaagg tataacttat tataactacg atgaatttga tgatctcgat 2040 gagagtcgga aatacatttt caacaagact gcagagtatc aagaagagat agatattctt 2100 aaagatattg gatccgccat ttctcaaatc actgacggtc aaatccaagc tactaccact 2160 gctaccaccg aagctaccac cactgctgcc ccatcttcca ccgttgaaac tgtttctcca 2220 tccagcaccg aaactatctc tcaacaaact gaaaatggtg ctgctaaggc cgctgtcggt 2280 atgggtgccg gtgctctagc tgctgctgct atgttgttat aa 2322 <210> 6 <211> 1656 <212> DNA <213> Xenopus laevis <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Xenopus laevis <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Xenopus laevis <400> 6 atgcactgtg aacgatttat ttgtatatta cgaataattg gtacgacttt gttcggagta 60 agtctgctac tagggatatc cgccgcttat atcgtgggat accaattcat tcagacggat 120 aattactact tttccttcgg tctctacggt gctattttag ctttacattt aatcattcag 180 tctctcttcg cattcctgga gcatcgtaag atgaagcggt cacttgaaac ccctataaaa 240 ttaaacaaga gcgtagcatt gtgtatcgct gcataccagg aagacgaaga ttatcttaga 300 aaatgtttgc tttctgtgaa acggctcaca taccccggta tgaaggtaat catggtgata 360 gatggtaact ctgatgacga tctatacatg atgaacatat tcagagagat catgggaaat 420 gatagctgcg ccacctatgt atggaagaat aattttcaca tgaagggacc aaacgagacc 480 gacgagactc accgcgagtc catgcagcat gtaacacaga tggtgctttc taacagaaat 540 gtctgtatca tgcaaaagtg gaacgggaaa cgcgaggtga tgtatactgc attcaaagcg 600 ctgggtcggt cggttgatta cgtccaagtg tgcgactcag atacagtcct tgatccagca 660 agctcggtgg agatggtcaa ggtgttggag gaagacatta tggtcggagg tgtaggcgga 720 gacgtgcaga tactcaataa gtacgactcc tggatcagtt tcttgagttc ggttcgttat 780 tggatggctt tcaatataga acgagcatgc caatcatact ttggctgtgt gcagtgtata 840 tcggggcctc tgggcatgta cagaaactca ctcttacatg agttcattga agactggtac 900 aatcaagaat tcctcggttc ccaatgctct ttcggtgatg atcggcattt gactaatcga 960 gtactatcat tgggctatgc tactaaatac actgcgcgca gtaagtgtct gacagaaacc 1020 cccacagaat atcttagatg gttgaaccaa caaaccaggt ggagtaagtc ctatttccgc 1080 gagtggttgt acaactcttt gtggttccac aaacaccatt tatggatgac ttatgaagcc 1140 gtaattacgg gtttcttccc cttcttctta atagcgacgg tgattcagct cttctatcgt 1200 ggtcgaattt ggaatatctt actctttcta ctcacagttc aattagttgg tctaataaag 1260 tcgtcgtttg caagtgcgtt gcgtggaaac atcgttatgg tctttatgag tttctacagt 1320 gttttataca tgagtagctt gctgccagca aagatgtttg caatcgccac gataaacaag 1380 gctggatggg gtactagtgg tagaaagacc atagttgtta attttattgg tttaatccct 1440 attacggtat ggttcacaat tttactaggg ggagtatgct acactatatg gcgggaaacc 1500 aagaagccgt ttagcgagtc tgagaagatc gttcttgcgg tcggtgctat attgtatgct 1560 tgttattggg tcatgttgct tacaatgtat gtcagtcttg tcatgaagtg cgggcgccgt 1620 aggaaggaac cacagcacga ccttgtttta gcataa 1656 <210> 7 <211> 1254 <212> DNA <213> Streptococcus zooepidemicus <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Streptococcus zooepidemicus <220> <223> Reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Streptococcus zooepidemicus <400> 7 atgaggacgt taaagaatct tatcactgtt gtagcgttca gtatattctg ggttttgctg 60 atctatgtaa atgtctatct tttcggtgcg aaggggtctc tatcaatata tggattcttg 120 ctaattgcgt atttgcttgt gaagatgagt ctttcattct tctataaacc attcaaaggt 180 cgagctggtc agtataaagt cgcagcgatt atcccttcat acaacgagga tgcagaatcg 240 ctgttggaaa ccttaaaatc tgttcaacaa caaacctatc cccttgctga aatttatgta 300 gttgatgatg gttccgcaga tgaaaccggt atcaagcgga tcgaggatta tgtcagggat 360 acaggcgact taagttcaaa tgtgattgtg catcgatcag aaaagaatca aggcaagaga 420 cacgcccaag catgggcatt tgaaagatct gatgcagatg tatttctaac agtcgatagt 480 gatacttaca tttatccgga cgcccttgaa gagttattga aaaccttcaa tgatccgaca 540 gtatttgccg caactggcca cttgaacgtt cgtaacaggc agactaatct attgaccaga 600 ctcactgata taagatacga caatgctttt ggcgtcgaac gtgctgctca aagtgtaact 660 ggtaatatac tggtgtgttc cgggccactg tccgtctatc gcagagaagt agtagtcccg 720 aacattgaca ggtatattaa tcagactttt ctgggtatcc ccgtgtcaat tggggacgac 780 cggtgtttaa cgaactacgc tactgatttg ggcaagactg tatatcagtc gacggctaaa 840 tgtattacag acgtcccaga taagatgtcg acttacctta aacagcagaa tagatggaat 900 aagtcattct ttagagaatc tatcatcagc gtgaagaaga tcatgaataa tccattcgtg 960 gcgctttgga ccattttgga ggtttccatg tttatgatgc ttgtctactc tgtcgttgat 1020 ttctttgtag gtaacgttcg agaatttgat tggctcagag ttcttgcttt cttagttatc 1080 atcttcatcg ttgcgctatg caggaatatt cattacatgc taaaacatcc gcttagcttc 1140 ttactcagcc ctttctacgg cgttttgcat ctcttcgttt tgcagccatt gaagctctac 1200 tccttattta ccattcgaaa cgctgattgg ggtacgcgca agaaactatt ataa 1254 <210> 8 <211> 568 <212> PRT <213> Paramecium bursaria Chlorella virus 1 <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus PBCV-1 <400> 8 Met Gly Lys Asn Ile Ile Ile Met Val Ser Trp Tyr Thr Ile Ile Thr 1 5 10 15 Ser Asn Leu Ile Ala Val Gly Gly Ala Ser Leu Ile Leu Ala Pro Ala 20 25 30 Ile Thr Gly Tyr Val Leu His Trp Asn Ile Ala Leu Ser Thr Ile Trp 35 40 45 Gly Val Ser Ala Tyr Gly Ile Phe Val Phe Gly Phe Phe Leu Ala Gln 50 55 60 Val Leu Phe Ser Glu Leu Asn Arg Lys Arg Leu Arg Lys Trp Ile Ser 65 70 75 80 Leu Arg Pro Lys Gly Trp Asn Asp Val Arg Leu Ala Val Ile Ile Ala 85 90 95 Gly Tyr Arg Glu Asp Pro Tyr Met Phe Gln Lys Cys Leu Glu Ser Val 100 105 110 Arg Asp Ser Asp Tyr Gly Asn Val Ala Arg Leu Ile Cys Val Ile Asp 115 120 125 Gly Asp Glu Asp Asp Asp Met Arg Met Ala Ala Val Tyr Lys Ala Ile 130 135 140 Tyr Asn Asp Asn Ile Lys Lys Pro Glu Phe Val Leu Cys Glu Ser Asp 145 150 155 160 Asp Lys Glu Gly Glu Arg Ile Asp Ser Asp Phe Ser Arg Asp Ile Cys 165 170 175 Val Leu Gln Pro His Arg Gly Lys Arg Glu Cys Leu Tyr Thr Gly Phe 180 185 190 Gln Leu Ala Lys Met Asp Pro Ser Val Asn Ala Val Val Leu Ile Asp 195 200 205 Ser Asp Thr Val Leu Glu Lys Asp Ala Ile Leu Glu Val Val Tyr Pro 210 215 220 Leu Ala Cys Asp Pro Glu Ile Gln Ala Val Ala Gly Glu Cys Lys Ile 225 230 235 240 Trp Asn Thr Asp Thr Leu Leu Ser Leu Leu Val Ala Trp Arg Tyr Tyr 245 250 255 Ser Ala Phe Cys Val Glu Arg Ser Ala Gln Ser Phe Phe Arg Thr Val 260 265 270 Gln Cys Val Gly Gly Pro Leu Gly Ala Tyr Lys Ile Asp Ile Ile Lys 275 280 285 Glu Ile Lys Asp Pro Trp Ile Ser Gln Arg Phe Leu Gly Gln Lys Cys 290 295 300 Thr Tyr Gly Asp Asp Arg Arg Leu Thr Asn Glu Ile Leu Met Arg Gly 305 310 315 320 Lys Lys Val Val Phe Thr Pro Phe Ala Val Gly Trp Ser Asp Ser Pro 325 330 335 Thr Asn Val Phe Arg Tyr Ile Val Gln Gln Thr Arg Trp Ser Lys Ser 340 345 350 Trp Cys Arg Glu Ile Trp Tyr Thr Leu Phe Ala Ala Trp Lys His Gly 355 360 365 Leu Ser Gly Ile Trp Leu Ala Phe Glu Cys Leu Tyr Gln Ile Thr Tyr 370 375 380 Phe Phe Leu Val Ile Tyr Leu Phe Ser Arg Leu Ala Val Glu Ala Asp 385 390 395 400 Pro Arg Ala Gln Thr Ala Thr Val Ile Val Ser Thr Thr Val Ala Leu 405 410 415 Ile Lys Cys Gly Tyr Phe Ser Phe Arg Ala Lys Asp Ile Arg Ala Phe 420 425 430 Tyr Phe Val Leu Tyr Thr Phe Val Tyr Phe Phe Cys Met Ile Pro Ala 435 440 445 Arg Ile Thr Ala Met Met Thr Leu Trp Asp Ile Gly Trp Gly Thr Arg 450 455 460 Gly Gly Asn Glu Lys Pro Ser Val Gly Thr Arg Val Ala Leu Trp Ala 465 470 475 480 Lys Gln Tyr Leu Ile Ala Tyr Met Trp Trp Ala Ala Val Val Gly Ala 485 490 495 Gly Val Tyr Ser Ile Val His Asn Trp Met Phe Asp Trp Asn Ser Leu 500 505 510 Ser Tyr Arg Phe Ala Leu Val Gly Ile Cys Ser Tyr Ile Val Phe Ile 515 520 525 Val Ile Val Leu Val Val Tyr Phe Thr Gly Lys Ile Thr Thr Trp Asn 530 535 540 Phe Thr Lys Leu Gln Lys Glu Leu Ile Glu Asp Arg Val Leu Tyr Asp 545 550 555 560 Ala Thr Thr Asn Ala Gln Ser Val 565 <210> 9 <211> 972 <212> PRT <213> Pasteurella multocida <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Pasteurella multocida <400> 9 Met Asn Thr Leu Ser Gln Ala Ile Lys Ala Tyr Asn Ser Asn Asp Tyr 1 5 10 15 Gln Leu Ala Leu Lys Leu Phe Glu Lys Ser Ala Glu Ile Tyr Gly Arg 20 25 30 Lys Ile Val Glu Phe Gln Ile Thr Lys Cys Lys Glu Lys Leu Ser Ala 35 40 45 His Pro Ser Val Asn Ser Ala His Leu Ser Val Asn Lys Glu Glu Lys 50 55 60 Val Asn Val Cys Asp Ser Pro Leu Asp Ile Ala Thr Gln Leu Leu Leu 65 70 75 80 Ser Asn Val Lys Lys Leu Val Leu Ser Asp Ser Glu Lys Asn Thr Leu 85 90 95 Lys Asn Lys Trp Lys Leu Leu Thr Glu Lys Lys Ser Glu Asn Ala Glu 100 105 110 Val Arg Ala Val Ala Leu Val Pro Lys Asp Phe Pro Lys Asp Leu Val 115 120 125 Leu Ala Pro Leu Pro Asp His Val Asn Asp Phe Thr Trp Tyr Lys Lys 130 135 140 Arg Lys Lys Arg Leu Gly Ile Lys Pro Glu His Gln His Val Gly Leu 145 150 155 160 Ser Ile Ile Val Thr Thr Phe Asn Arg Pro Ala Ile Leu Ser Ile Thr 165 170 175 Leu Ala Cys Leu Val Asn Gln Lys Thr His Tyr Pro Phe Glu Val Ile 180 185 190 Val Thr Asp Asp Gly Ser Gln Glu Asp Leu Ser Pro Ile Ile Arg Gln 195 200 205 Tyr Glu Asn Lys Leu Asp Ile Arg Tyr Val Arg Gln Lys Asp Asn Gly 210 215 220 Phe Gln Ala Ser Ala Ala Arg Asn Met Gly Leu Arg Leu Ala Lys Tyr 225 230 235 240 Asp Phe Ile Gly Leu Leu Asp Cys Asp Met Ala Pro Asn Pro Leu Trp 245 250 255 Val His Ser Tyr Val Ala Glu Leu Leu Glu Asp Asp Asp Leu Thr Ile 260 265 270 Ile Gly Pro Arg Lys Tyr Ile Asp Thr Gln His Ile Asp Pro Lys Asp 275 280 285 Phe Leu Asn Asn Ala Ser Leu Leu Glu Ser Leu Pro Glu Val Lys Thr 290 295 300 Asn Asn Ser Val Ala Ala Lys Gly Glu Gly Thr Val Ser Leu Asp Trp 305 310 315 320 Arg Leu Glu Gln Phe Glu Lys Thr Glu Asn Leu Arg Leu Ser Asp Ser 325 330 335 Pro Phe Arg Phe Phe Ala Ala Gly Asn Val Ala Phe Ala Lys Lys Trp 340 345 350 Leu Asn Lys Ser Gly Phe Phe Asp Glu Glu Phe Asn His Trp Gly Gly 355 360 365 Glu Asp Val Glu Phe Gly Tyr Arg Leu Phe Arg Tyr Gly Ser Phe Phe 370 375 380 Lys Thr Ile Asp Gly Ile Met Ala Tyr His Gln Glu Pro Pro Gly Lys 385 390 395 400 Glu Asn Glu Thr Asp Arg Glu Ala Gly Lys Asn Ile Thr Leu Asp Ile 405 410 415 Met Arg Glu Lys Val Pro Tyr Ile Tyr Arg Lys Leu Leu Pro Ile Glu 420 425 430 Asp Ser His Ile Asn Arg Val Pro Leu Val Ser Ile Tyr Ile Pro Ala 435 440 445 Tyr Asn Cys Ala Asn Tyr Ile Gln Arg Cys Val Asp Ser Ala Leu Asn 450 455 460 Gln Thr Val Val Asp Leu Glu Val Cys Ile Cys Asn Asp Gly Ser Thr 465 470 475 480 Asp Asn Thr Leu Glu Val Ile Asn Lys Leu Tyr Gly Asn Asn Pro Arg 485 490 495 Val Arg Ile Met Ser Lys Pro Asn Gly Gly Ile Ala Ser Ala Ser Asn 500 505 510 Ala Ala Val Ser Phe Ala Lys Gly Tyr Tyr Ile Gly Gln Leu Asp Ser 515 520 525 Asp Asp Tyr Leu Glu Pro Asp Ala Val Glu Leu Cys Leu Lys Glu Phe 530 535 540 Leu Lys Asp Lys Thr Leu Ala Cys Val Tyr Thr Thr Asn Arg Asn Val 545 550 555 560 Asn Pro Asp Gly Ser Leu Ile Ala Asn Gly Tyr Asn Trp Pro Glu Phe 565 570 575 Ser Arg Glu Lys Leu Thr Thr Ala Met Ile Ala His His Phe Arg Met 580 585 590 Phe Thr Ile Arg Ala Trp His Leu Thr Asp Gly Phe Asn Glu Lys Ile 595 600 605 Glu Asn Ala Val Asp Tyr Asp Met Phe Leu Lys Leu Ser Glu Val Gly 610 615 620 Lys Phe Lys His Leu Asn Lys Ile Cys Tyr Asn Arg Val Leu His Gly 625 630 635 640 Asp Asn Thr Ser Ile Lys Lys Leu Gly Ile Gln Lys Lys Asn His Phe 645 650 655 Val Val Val Asn Gln Ser Leu Asn Arg Gln Gly Ile Thr Tyr Tyr Asn 660 665 670 Tyr Asp Glu Phe Asp Asp Leu Asp Glu Ser Arg Lys Tyr Ile Phe Asn 675 680 685 Lys Thr Ala Glu Tyr Gln Glu Glu Ile Asp Ile Leu Lys Asp Ile Lys 690 695 700 Ile Ile Gln Asn Lys Asp Ala Lys Ile Ala Val Ser Ile Phe Tyr Pro 705 710 715 720 Asn Thr Leu Asn Gly Leu Val Lys Lys Leu Asn Asn Ile Ile Glu Tyr 725 730 735 Asn Lys Asn Ile Phe Val Ile Val Leu His Val Asp Lys Asn His Leu 740 745 750 Thr Pro Asp Ile Lys Lys Glu Ile Leu Ala Phe Tyr His Lys His Gln 755 760 765 Val Asn Ile Leu Leu Asn Asn Asp Ile Ser Tyr Tyr Thr Ser Asn Arg 770 775 780 Leu Ile Lys Thr Glu Ala His Leu Ser Asn Ile Asn Lys Leu Ser Gln 785 790 795 800 Leu Asn Leu Asn Cys Glu Tyr Ile Ile Phe Asp Asn His Asp Ser Leu 805 810 815 Phe Val Lys Asn Asp Ser Tyr Ala Tyr Met Lys Lys Tyr Asp Val Gly 820 825 830 Met Asn Phe Ser Ala Leu Thr His Asp Trp Ile Glu Lys Ile Asn Ala 835 840 845 His Pro Pro Phe Lys Lys Leu Ile Lys Thr Tyr Phe Asn Asp Asn Asp 850 855 860 Leu Lys Ser Met Asn Val Lys Gly Ala Ser Gln Gly Met Phe Met Thr 865 870 875 880 Tyr Ala Leu Ala His Glu Leu Leu Thr Ile Ile Lys Glu Val Ile Thr 885 890 895 Ser Cys Gln Ser Ile Asp Ser Val Pro Glu Tyr Asn Thr Glu Asp Ile 900 905 910 Trp Phe Gln Phe Ala Leu Leu Ile Leu Glu Lys Lys Thr Gly His Val 915 920 925 Phe Asn Lys Thr Ser Thr Leu Thr Tyr Met Pro Trp Glu Arg Lys Leu 930 935 940 Gln Trp Thr Asn Glu Gln Ile Glu Ser Ala Lys Arg Gly Glu Asn Ile 945 950 955 960 Pro Val Asn Lys Phe Ile Ile Asn Ser Ile Thr Leu 965 970 <210> 10 <211> 551 <212> PRT <213> Xenopus laevis <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Xenopus laevis <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Xenopus laevis <400> 10 Met His Cys Glu Arg Phe Ile Cys Ile Leu Arg Ile Ile Gly Thr Thr 1 5 10 15 Leu Phe Gly Val Ser Leu Leu Leu Gly Ile Ser Ala Ala Tyr Ile Val 20 25 30 Gly Tyr Gln Phe Ile Gln Thr Asp Asn Tyr Tyr Phe Ser Phe Gly Leu 35 40 45 Tyr Gly Ala Ile Leu Ala Leu His Leu Ile Ile Gln Ser Leu Phe Ala 50 55 60 Phe Leu Glu His Arg Lys Met Lys Arg Ser Leu Glu Thr Pro Ile Lys 65 70 75 80 Leu Asn Lys Ser Val Ala Leu Cys Ile Ala Ala Tyr Gln Glu Asp Glu 85 90 95 Asp Tyr Leu Arg Lys Cys Leu Leu Ser Val Lys Arg Leu Thr Tyr Pro 100 105 110 Gly Met Lys Val Ile Met Val Ile Asp Gly Asn Ser Asp Asp Asp Leu 115 120 125 Tyr Met Met Asn Ile Phe Arg Glu Ile Met Gly Asn Asp Ser Cys Ala 130 135 140 Thr Tyr Val Trp Lys Asn Asn Phe His Met Lys Gly Pro Asn Glu Thr 145 150 155 160 Asp Glu Thr His Arg Glu Ser Met Gln His Val Thr Gln Met Val Leu 165 170 175 Ser Asn Arg Asn Val Cys Ile Met Gln Lys Trp Asn Gly Lys Arg Glu 180 185 190 Val Met Tyr Thr Ala Phe Lys Ala Leu Gly Arg Ser Val Asp Tyr Val 195 200 205 Gln Val Cys Asp Ser Asp Thr Val Leu Asp Pro Ala Ser Ser Val Glu 210 215 220 Met Val Lys Val Leu Glu Glu Asp Ile Met Val Gly Gly Val Gly Gly 225 230 235 240 Asp Val Gln Ile Leu Asn Lys Tyr Asp Ser Trp Ile Ser Phe Leu Ser 245 250 255 Ser Val Arg Tyr Trp Met Ala Phe Asn Ile Glu Arg Ala Cys Gln Ser 260 265 270 Tyr Phe Gly Cys Val Gln Cys Ile Ser Gly Pro Leu Gly Met Tyr Arg 275 280 285 Asn Ser Leu Leu His Glu Phe Ile Glu Asp Trp Tyr Asn Gln Glu Phe 290 295 300 Leu Gly Ser Gln Cys Ser Phe Gly Asp Asp Arg His Leu Thr Asn Arg 305 310 315 320 Val Leu Ser Leu Gly Tyr Ala Thr Lys Tyr Thr Ala Arg Ser Lys Cys 325 330 335 Leu Thr Glu Thr Pro Thr Glu Tyr Leu Arg Trp Leu Asn Gln Gln Thr 340 345 350 Arg Trp Ser Lys Ser Tyr Phe Arg Glu Trp Leu Tyr Asn Ser Leu Trp 355 360 365 Phe His Lys His His Leu Trp Met Thr Tyr Glu Ala Val Ile Thr Gly 370 375 380 Phe Phe Pro Phe Phe Leu Ile Ala Thr Val Ile Gln Leu Phe Tyr Arg 385 390 395 400 Gly Arg Ile Trp Asn Ile Leu Leu Phe Leu Leu Thr Val Gln Leu Val 405 410 415 Gly Leu Ile Lys Ser Ser Phe Ala Ser Ala Leu Arg Gly Asn Ile Val 420 425 430 Met Val Phe Met Ser Phe Tyr Ser Val Leu Tyr Met Ser Ser Leu Leu 435 440 445 Pro Ala Lys Met Phe Ala Ile Ala Thr Ile Asn Lys Ala Gly Trp Gly 450 455 460 Thr Ser Gly Arg Lys Thr Ile Val Val Asn Phe Ile Gly Leu Ile Pro 465 470 475 480 Ile Thr Val Trp Phe Thr Ile Leu Leu Gly Gly Val Cys Tyr Thr Ile 485 490 495 Trp Arg Glu Thr Lys Lys Pro Phe Ser Glu Ser Glu Lys Ile Val Leu 500 505 510 Ala Val Gly Ala Ile Leu Tyr Ala Cys Tyr Trp Val Met Leu Leu Thr 515 520 525 Met Tyr Val Ser Leu Val Met Lys Cys Gly Arg Arg Arg Lys Glu Pro 530 535 540 Gln His Asp Leu Val Leu Ala 545 550 <210> 11 <211> 417 <212> PRT <213> Streptococcus zooepidemicus <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Streptococcus zooepidemicus <220> <223> Amino acid sequence of hyaluronan synthase (HASA) originating from Streptococcus zooepidemicus <400> 11 Met Arg Thr Leu Lys Asn Leu Ile Thr Val Val Ala Phe Ser Ile Phe 1 5 10 15 Trp Val Leu Leu Ile Tyr Val Asn Val Tyr Leu Phe Gly Ala Lys Gly 20 25 30 Ser Leu Ser Ile Tyr Gly Phe Leu Leu Ile Ala Tyr Leu Leu Val Lys 35 40 45 Met Ser Leu Ser Phe Phe Tyr Lys Pro Phe Lys Gly Arg Ala Gly Gln 50 55 60 Tyr Lys Val Ala Ala Ile Ile Pro Ser Tyr Asn Glu Asp Ala Glu Ser 65 70 75 80 Leu Leu Glu Thr Leu Lys Ser Val Gln Gln Gln Thr Tyr Pro Leu Ala 85 90 95 Glu Ile Tyr Val Val Asp Asp Gly Ser Ala Asp Glu Thr Gly Ile Lys 100 105 110 Arg Ile Glu Asp Tyr Val Arg Asp Thr Gly Asp Leu Ser Ser Asn Val 115 120 125 Ile Val His Arg Ser Glu Lys Asn Gln Gly Lys Arg His Ala Gln Ala 130 135 140 Trp Ala Phe Glu Arg Ser Asp Ala Asp Val Phe Leu Thr Val Asp Ser 145 150 155 160 Asp Thr Tyr Ile Tyr Pro Asp Ala Leu Glu Glu Leu Leu Lys Thr Phe 165 170 175 Asn Asp Pro Thr Val Phe Ala Ala Thr Gly His Leu Asn Val Arg Asn 180 185 190 Arg Gln Thr Asn Leu Leu Thr Arg Leu Thr Asp Ile Arg Tyr Asp Asn 195 200 205 Ala Phe Gly Val Glu Arg Ala Ala Gln Ser Val Thr Gly Asn Ile Leu 210 215 220 Val Cys Ser Gly Pro Leu Ser Val Tyr Arg Arg Glu Val Val Val Pro 225 230 235 240 Asn Ile Asp Arg Tyr Ile Asn Gln Thr Phe Leu Gly Ile Pro Val Ser 245 250 255 Ile Gly Asp Asp Arg Cys Leu Thr Asn Tyr Ala Thr Asp Leu Gly Lys 260 265 270 Thr Val Tyr Gln Ser Thr Ala Lys Cys Ile Thr Asp Val Pro Asp Lys 275 280 285 Met Ser Thr Tyr Leu Lys Gln Gln Asn Arg Trp Asn Lys Ser Phe Phe 290 295 300 Arg Glu Ser Ile Ile Ser Val Lys Lys Ile Met Asn Asn Pro Phe Val 305 310 315 320 Ala Leu Trp Thr Ile Leu Glu Val Ser Met Phe Met Met Leu Val Tyr 325 330 335 Ser Val Val Asp Phe Phe Val Gly Asn Val Arg Glu Phe Asp Trp Leu 340 345 350 Arg Val Leu Ala Phe Leu Val Ile Ile Phe Ile Val Ala Leu Cys Arg 355 360 365 Asn Ile His Tyr Met Leu Lys His Pro Leu Ser Phe Leu Leu Ser Pro 370 375 380 Phe Tyr Gly Val Leu His Leu Phe Val Leu Gln Pro Leu Lys Leu Tyr 385 390 395 400 Ser Leu Phe Thr Ile Arg Asn Ala Asp Trp Gly Thr Arg Lys Lys Leu 405 410 415 Leu <210> 12 <211> 1446 <212> DNA <213> Arabidopsis thaliana <220> <223> Nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Arabidopsis thaliana <220> <223> Nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Arabidopsis thaliana <400> 12 atggtcaaaa tatgctgtat cggggctgga tatgtcggtg ggcctacaat ggccgttatg 60 gccctaaagt gtccagagat tgaagtcgtg gtagttgata tatcggagcc acgaattaac 120 gcatggaact cagaccgtct accaatttat gagccagggt tagaggatgt cgtcaaacag 180 tgtagaggga agaatttgtt cttctctaca gatgtagaga agcatgtatt tgagtcagat 240 atagtgtttg tttcggtaaa cactcctacg aaaacgcagg gtctgggtgc aggtaaagca 300 gcagatttga catattggga gtccgctgct cgcatgatag ctgatgtgag caaatcgtcg 360 aaaatcgtcg ttgaaaagag tacagtacca gttcgtacag ccgaggctat agaaaagatt 420 ttaacgcaca attcgaaggg tatcgaattc cagatcttat caaatccaga attcttggcc 480 gaagggacgg cgattaaaga cttatataac cctgatagag ttctaatcgg tggcagggac 540 accgctgcgg gacaaaaggc cattaaggcg ttgcgtgacg tgtatgccca ctgggttcct 600 gttgagcaaa taatctgtac taatttatgg agtgccgagc tatcaaagtt ggctgcgaac 660 gcatttctag ctcaaaggat aagttcagta aacgcaatgt cagcgctttg tgaggcaact 720 ggtgctgacg taacccaagt cgctcacgcc gttggaaccg acactagaat tggaccgaag 780 tttcttaacg catccgtagg cttcggcgga tcttgctttc agaaagacat cctgaatctt 840 atttacatct gcgaatgcaa tggtcttcca gaagcagcca attattggaa acaggtagtc 900 aaggtaaatg actaccaaaa gattaggttt gctaatcgag tcgtatcttc tatgttcaac 960 accgtctccg gtaagaaaat tgctattttg ggatttgcgt tcaagaagga caccggcgac 1020 acgcgtgaaa ctcctgccat agatgtgtgt aatcgcctcg tggctgataa agcaaagctg 1080 tcgatctatg atccgcaagt attagaagag cagatccgcc gtgatctgtc catggcccga 1140 ttcgattggg accatccagt cccactccag cagatcaaag ctgaaggtat ctccgaacag 1200 gttaacgttg tgtccgacgc ctacgaggct acgaaggatg ctcatggttt atgtgtttta 1260 accgaatggg acgaattcaa gtcacttgat tttaagaaga tctttgataa tatgcagaaa 1320 cccgctttcg ttttcgacgg aagaaacgtg gtcgacgctg tgaaattgag agaaattgga 1380 ttcatagtat attccatagg taaacctctg gatagttggc tcaaggatat gccggctgtt 1440 gcataa 1446 <210> 13 <211> 1166 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV-1 <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV-1 <400> 13 atgagtagaa ttgctgtcgt tggatgcggt tacgtgggta cggcctgcgc cgtacttttg 60 gcgcagaaga acgaagttat cgttttggat atctcggagg accgggtaca actgattaag 120 aataagaagt cacctataga agataaggaa atcgaagaat tcctggagac gaaagatttg 180 aatctaacag cgacgacgga taaggtgctc gcctatgaga atgctgaatt cgttataata 240 gctacaccga ccgattacga tgtcgtcact agatatttca acactaagtc tgttgaaaat 300 gtgataggcg atgtcattaa gaacactcag acacacccta ctatagtgat caagagtact 360 attccaatcg gtttcgtgga taaagttaga gaacagttcg attatcaaaa tattatcttc 420 tcgccggaat tcttgagaga aggacgagca ttatatgata atctttaccc ctcccgtatc 480 atcgtcggtg atgactcccc aattgcctta aaattcgcga atctcttggt cgagggtagc 540 aaaactccac tagctcccgt attaactatg ggtacgcgag aggccgaagc tgtaaaacta 600 ttttcaaata catatttggc tatgagagta gcatacttca atgagctaga cacatttgca 660 atgtcccatg gtatgaacgc aaaagagatt atcgacggag tcacactaga accaaggata 720 gggcagggtt attcgaatcc ttcatttggg tatggagcct attgtttccc aaaagataca 780 aagcaattgt tggctaattt tgaaggggtt ccacaagata ttattggcgc aatcgtagag 840 tctaacgaaa ctagaaagga ggttattgtt tctgaggtcg agaacagatt tccgaccaca 900 gttggcgtat ataagttggc tgctaaagct ggttcggata atttcaggtc tagtgcgata 960 gtcgatatca tggaaaggtt ggctaataag ggctaccaca tcaaaatatt tgaacctacc 1020 gttgaacaat ttgagaattt tgaggtagat aataatctca caacttttgc aaccgagtct 1080 gatgtaataa ttgcgaacag agttccagtc gaacatcgca ttctgtttgg gaagaagtta 1140 ataacacggg atgtctatgg ggataa 1166 <210> 14 <211> 1170 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV-1 <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV-1 <400> 14 atgtcacgaa tcgcagtagt tggctgtggg tacgtgggaa ccgcatgcgc tgtactgctg 60 gcgcaaaaga atgaggtaat cgttcttgat attagtgagg atagggtcca actaatcaag 120 aacaagaaat ccccgatcga agataaggag attgaagaat tcttggaaac aaaagaccta 180 aacttaactg caacaactga caaagtttta gcctacgaaa acgctgagtt tgtgattata 240 gcgacaccca cagattatga cgtagttacc agatacttta acacgaagtc cgtagagaac 300 gtcattggag atgttataaa gaatactcag actcatccta cgatagtaat aaagtcaacc 360 attcccatag gtttcgtaga taaggttagg gagcaattcg attaccagaa cattatattt 420 tcgccagaat ttctgagaga gggtcgcgcc ctgtatgata atctatatcc atcacggatt 480 atagtgggcg atgactctcc gattgcactt aagtttgcta atcttttagt tgagggctcc 540 aaaactccgc tcgccccagt acttacgatg ggtacacgtg aggctgaagc tgtcaagctg 600 ttttcaaaca cataccttgc tatgcgagtc gcatacttta acgaactaga tacctttgct 660 atgtcgcacg gtatgaatgc taaagaaatc atagatggcg taacgttgga gcctcggata 720 ggtcaaggat attccaatcc atcttttggc tacggtgcgt attgtttccc aaaggacacg 780 aagcaattat tagctaactt tgagggtgtt ccgcaagata taattggggc gatagtagaa 840 agcaatgaaa cacggaagga ggtaatcgtg agtgaagtgg aaaaccgatt ccccactacg 900 gtcggcgttt acaaattagc cgccaaggct ggttccgaca atttccgatc cagcgcaata 960 gtagatatta tggaaagatt agctaataag ggataccaca ttaaaatctt tgaacctact 1020 gtcgaacagt tcgagaactt cgaggttgat aataacttga cgactttcgc aacggagagc 1080 gatgtaatta ttgcaaaccg cgtacctgtg gaacatcgaa ttttgttcgg aaagaagctg 1140 attacacgcg atgtatatgg cgataactaa 1170 <210> 15 <211> 1199 <212> DNA <213> streptococcus zooepidemicus <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Streptococcus zooepidemicus <220> <223> Reencoded nucleic acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Streptococcus zooepidemicus <400> 15 atgaagatat cggtagcggg ttcggggtac gtggggttat ccttgtcaat cttgcttgcc 60 caacataacg atgtgactgt tgtagatata atcgacgaaa aggtacggct aattaaccag 120 ggcatatctc cgattaagga tgcggacatt gaggaatatc tgaagaatgc accgttgaat 180 cttacggcta cactagacgg agcttcggct tatagtaatg ctgatctgat tataatcgca 240 acgccaacta attacgattc agaacgcaat tatttcgaca ccagacacgt tgaagaagta 300 attgagcaag tattggattt aaatgcctcc gctactataa tcatcaagag taccataccc 360 ttgggtttta ttaaacacgt aagagagaaa taccaaacag acagaatcat cttttctcca 420 gagttcttaa gagagtcaaa ggcattgtac gataacttat acccctctcg tataatagtc 480 agttatgaga aggatgactc tccaagagtt atacaagcag ctaaggcgtt cgcgggttta 540 ttaaaagagg gggcaaagag caaggatacc ccagttctgt ttatgggctc tcaagaagct 600 gaagctgtca agctgtttgc taatactttt ctcgccatga gggtcagtta cttcaacgag 660 cttgacactt atagcgaatc aaaaggacta gacgcccaaa gagttataga aggcgtctgc 720 catgatcaaa ggataggtaa tcattacaat aatccatcct tcggatatgg cggttattgt 780 ttacccaaag actcaaagca acttttggct aattatagag gcatacctca gtctctaatg 840 tctgccatcg ttgaatcgaa caagatccgt aagtcgtatt tagctgaaca aatattagat 900 agggcttctt cacaaaagca ggctggtgta cctttaacca taggatttta ccgtttgatt 960 atgaagtcca actccgataa ctttagagaa tcagccatta aagatattat tgacatcatt 1020 aatgactacg gtgtcaatat tgtcatttat gaacctatgt tgggagaaga cattggttat 1080 agagtcgtta aagatttgga acagtttaag aacgaaagta caattattgt tagtaacagg 1140 tttgaagatg atttaggtga tgttattgat aaagtttata cacgtgacgt ctttggtag 1199 <210> 16 <211> 481 <212> PRT <213> Arabidopsis thaliana <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Arabidopsis thaliana <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Arabidopsis thaliana <400> 16 Met Val Lys Ile Cys Cys Ile Gly Ala Gly Tyr Val Gly Gly Pro Thr 1 5 10 15 Met Ala Val Met Ala Leu Lys Cys Pro Glu Ile Glu Val Val Val Val 20 25 30 Asp Ile Ser Glu Pro Arg Ile Asn Ala Trp Asn Ser Asp Arg Leu Pro 35 40 45 Ile Tyr Glu Pro Gly Leu Glu Asp Val Val Lys Gln Cys Arg Gly Lys 50 55 60 Asn Leu Phe Phe Ser Thr Asp Val Glu Lys His Val Phe Glu Ser Asp 65 70 75 80 Ile Val Phe Val Ser Val Asn Thr Pro Thr Lys Thr Gln Gly Leu Gly 85 90 95 Ala Gly Lys Ala Ala Asp Leu Thr Tyr Trp Glu Ser Ala Ala Arg Met 100 105 110 Ile Ala Asp Val Ser Lys Ser Ser Lys Ile Val Val Glu Lys Ser Thr 115 120 125 Val Pro Val Arg Thr Ala Glu Ala Ile Glu Lys Ile Leu Thr His Asn 130 135 140 Ser Lys Gly Ile Glu Phe Gln Ile Leu Ser Asn Pro Glu Phe Leu Ala 145 150 155 160 Glu Gly Thr Ala Ile Lys Asp Leu Tyr Asn Pro Asp Arg Val Leu Ile 165 170 175 Gly Gly Arg Asp Thr Ala Ala Gly Gln Lys Ala Ile Lys Ala Leu Arg 180 185 190 Asp Val Tyr Ala His Trp Val Pro Val Glu Gln Ile Ile Cys Thr Asn 195 200 205 Leu Trp Ser Ala Glu Leu Ser Lys Leu Ala Ala Asn Ala Phe Leu Ala 210 215 220 Gln Arg Ile Ser Ser Val Asn Ala Met Ser Ala Leu Cys Glu Ala Thr 225 230 235 240 Gly Ala Asp Val Thr Gln Val Ala His Ala Val Gly Thr Asp Thr Arg 245 250 255 Ile Gly Pro Lys Phe Leu Asn Ala Ser Val Gly Phe Gly Gly Ser Cys 260 265 270 Phe Gln Lys Asp Ile Leu Asn Leu Ile Tyr Ile Cys Glu Cys Asn Gly 275 280 285 Leu Pro Glu Ala Ala Asn Tyr Trp Lys Gln Val Val Lys Val Asn Asp 290 295 300 Tyr Gln Lys Ile Arg Phe Ala Asn Arg Val Val Ser Ser Met Phe Asn 305 310 315 320 Thr Val Ser Gly Lys Lys Ile Ala Ile Leu Gly Phe Ala Phe Lys Lys 325 330 335 Asp Thr Gly Asp Thr Arg Glu Thr Pro Ala Ile Asp Val Cys Asn Arg 340 345 350 Leu Val Ala Asp Lys Ala Lys Leu Ser Ile Tyr Asp Pro Gln Val Leu 355 360 365 Glu Glu Gln Ile Arg Arg Asp Leu Ser Met Ala Arg Phe Asp Trp Asp 370 375 380 His Pro Val Pro Leu Gln Gln Ile Lys Ala Glu Gly Ile Ser Glu Gln 385 390 395 400 Val Asn Val Val Ser Asp Ala Tyr Glu Ala Thr Lys Asp Ala His Gly 405 410 415 Leu Cys Val Leu Thr Glu Trp Asp Glu Phe Lys Ser Leu Asp Phe Lys 420 425 430 Lys Ile Phe Asp Asn Met Gln Lys Pro Ala Phe Val Phe Asp Gly Arg 435 440 445 Asn Val Val Asp Ala Val Lys Leu Arg Glu Ile Gly Phe Ile Val Tyr 450 455 460 Ser Ile Gly Lys Pro Leu Asp Ser Trp Leu Lys Asp Met Pro Ala Val 465 470 475 480 Ala <210> 17 <211> 389 <212> PRT <213> Paramecium bursaria Chlorella virus 1 <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV1 <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Chlorella virus PBCV1 <400> 17 Met Ser Arg Ile Ala Val Val Gly Cys Gly Tyr Val Gly Thr Ala Cys 1 5 10 15 Ala Val Leu Leu Ala Gln Lys Asn Glu Val Ile Val Leu Asp Ile Ser 20 25 30 Glu Asp Arg Val Gln Leu Ile Lys Asn Lys Lys Ser Pro Ile Glu Asp 35 40 45 Lys Glu Ile Glu Glu Phe Leu Glu Thr Lys Asp Leu Asn Leu Thr Ala 50 55 60 Thr Thr Asp Lys Val Leu Ala Tyr Glu Asn Ala Glu Phe Val Ile Ile 65 70 75 80 Ala Thr Pro Thr Asp Tyr Asp Val Val Thr Arg Tyr Phe Asn Thr Lys 85 90 95 Ser Val Glu Asn Val Ile Gly Asp Val Ile Lys Asn Thr Gln Thr His 100 105 110 Pro Thr Ile Val Ile Lys Ser Thr Ile Pro Ile Gly Phe Val Asp Lys 115 120 125 Val Arg Glu Gln Phe Asp Tyr Gln Asn Ile Ile Phe Ser Pro Glu Phe 130 135 140 Leu Arg Glu Gly Arg Ala Leu Tyr Asp Asn Leu Tyr Pro Ser Arg Ile 145 150 155 160 Ile Val Gly Asp Asp Ser Pro Ile Ala Leu Lys Phe Ala Asn Leu Leu 165 170 175 Val Glu Gly Ser Lys Thr Pro Leu Ala Pro Val Leu Thr Met Gly Thr 180 185 190 Arg Glu Ala Glu Ala Val Lys Leu Phe Ser Asn Thr Tyr Leu Ala Met 195 200 205 Arg Val Ala Tyr Phe Asn Glu Leu Asp Thr Phe Ala Met Ser His Gly 210 215 220 Met Asn Ala Lys Glu Ile Ile Asp Gly Val Thr Leu Glu Pro Arg Ile 225 230 235 240 Gly Gln Gly Tyr Ser Asn Pro Ser Phe Gly Tyr Gly Ala Tyr Cys Phe 245 250 255 Pro Lys Asp Thr Lys Gln Leu Leu Ala Asn Phe Glu Gly Val Pro Gln 260 265 270 Asp Ile Ile Gly Ala Ile Val Glu Ser Asn Glu Thr Arg Lys Glu Val 275 280 285 Ile Val Ser Glu Val Glu Asn Arg Phe Pro Thr Thr Val Gly Val Tyr 290 295 300 Lys Leu Ala Ala Lys Ala Gly Ser Asp Asn Phe Arg Ser Ser Ala Ile 305 310 315 320 Val Asp Ile Met Glu Arg Leu Ala Asn Lys Gly Tyr His Ile Lys Ile 325 330 335 Phe Glu Pro Thr Val Glu Gln Phe Glu Asn Phe Glu Val Asp Asn Asn 340 345 350 Leu Thr Thr Phe Ala Thr Glu Ser Asp Val Ile Ile Ala Asn Arg Val 355 360 365 Pro Val Glu His Arg Ile Leu Phe Gly Lys Lys Leu Ile Thr Arg Asp 370 375 380 Val Tyr Gly Asp Asn 385 <210> 18 <211> 401 <212> PRT <213> Streptococcus zooepidemicus <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Streptococcus zooepidemicus <220> <223> Amino acid sequence of UDP-Glucose dehydrogenase (HASB) originating from Streptococcus zooepidemicus <400> 18 Met Lys Ile Ser Val Ala Gly Ser Gly Tyr Val Gly Leu Ser Leu Ser 1 5 10 15 Ile Leu Leu Ala Gln His Asn Asp Val Thr Val Val Asp Ile Ile Asp 20 25 30 Glu Lys Val Arg Leu Ile Asn Gln Gly Ile Ser Pro Ile Lys Asp Ala 35 40 45 Asp Ile Glu Glu Tyr Leu Lys Asn Ala Pro Leu Asn Leu Thr Ala Thr 50 55 60 Leu Asp Gly Ala Ser Ala Tyr Ser Asn Ala Asp Leu Ile Ile Ile Ala 65 70 75 80 Thr Pro Thr Asn Tyr Asp Ser Glu Arg Asn Tyr Phe Asp Thr Arg His 85 90 95 Val Glu Glu Val Ile Glu Gln Val Leu Asp Leu Asn Ala Ser Ala Thr 100 105 110 Ile Ile Ile Lys Ser Thr Ile Pro Leu Gly Phe Ile Lys His Val Arg 115 120 125 Glu Lys Tyr Gln Thr Asp Arg Ile Ile Phe Ser Pro Glu Phe Leu Arg 130 135 140 Glu Ser Lys Ala Leu Tyr Asp Asn Leu Tyr Pro Ser Arg Ile Ile Val 145 150 155 160 Ser Tyr Glu Lys Asp Asp Ser Pro Arg Val Ile Gln Ala Ala Lys Ala 165 170 175 Phe Ala Gly Leu Leu Lys Glu Gly Ala Lys Ser Lys Asp Thr Pro Val 180 185 190 Leu Phe Met Gly Ser Gln Glu Ala Glu Ala Val Lys Leu Phe Ala Asn 195 200 205 Thr Phe Leu Ala Met Arg Val Ser Tyr Phe Asn Glu Leu Asp Thr Tyr 210 215 220 Ser Glu Ser Lys Gly Leu Asp Ala Gln Arg Val Ile Glu Gly Val Cys 225 230 235 240 His Asp Gln Arg Ile Gly Asn His Tyr Asn Asn Pro Ser Phe Gly Tyr 245 250 255 Gly Gly Tyr Cys Leu Pro Lys Asp Ser Lys Gln Leu Leu Ala Asn Tyr 260 265 270 Arg Gly Ile Pro Gln Ser Leu Met Ser Ala Ile Val Glu Ser Asn Lys 275 280 285 Ile Arg Lys Ser Tyr Leu Ala Glu Gln Ile Leu Asp Arg Ala Ser Ser 290 295 300 Gln Lys Gln Ala Gly Val Pro Leu Thr Ile Gly Phe Tyr Arg Leu Ile 305 310 315 320 Met Lys Ser Asn Ser Asp Asn Phe Arg Glu Ser Ala Ile Lys Asp Ile 325 330 335 Ile Asp Ile Ile Asn Asp Tyr Gly Val Asn Ile Val Ile Tyr Glu Pro 340 345 350 Met Leu Gly Glu Asp Ile Gly Tyr Arg Val Val Lys Asp Leu Glu Gln 355 360 365 Phe Lys Asn Glu Ser Thr Ile Ile Val Ser Asn Arg Phe Glu Asp Asp 370 375 380 Leu Gly Asp Val Ile Asp Lys Val Tyr Thr Arg Asp Val Phe Gly Arg 385 390 395 400 Asp <210> 19 <211> 1326 <212> DNA <213> Bothrops atrox <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal <400> 19 atgcaattta gcacagtcgc atcagtagcc ttcgttgcct tggccaactt cgtggcagca 60 ccgatgtatc cgaacgaacc gttcttagtc ttttggaacg cgcctacaac tcagtgtaga 120 cttcgatata aggttgacct tgatctgaag acattccata tcgtgacaaa tgctaatgac 180 tcgctgtcag gatcggctgt cacgattttc tatcccacgc acttaggggt ttacccacat 240 attgatgaca gggggcactt cttcaatggc atcatacccc aaaatgaatc cctggtaaag 300 catttaaaca aatctaaatc agatattaat cgaatgattc ccttaagaac attccacggg 360 ctgggagtca tagactggga aaactggcgg ccacagtggg ataggaattg gggaagtaag 420 aacgtttata ggaatagatc aatccaattc gcgcgtgatc tccacccaga gcttagtgag 480 gacaagatta aacgcttggc aaaacaggaa ttcgagaaag ctgcaaagag ttttatgagg 540 gatacactat tattagccga ggagatgcga ccagacggct actggggata ctatctgtac 600 cccgattgtc acaattacaa ttataagact aagccagatc agtacacagg agagtgccct 660 gacatcgaga tgtcacgtaa caatcaactc ttgtggcttt ggcgggatag cactgccctt 720 ttccccaata tctacttaga gactatacta agaagctctg acaatgccct gaagtttgtg 780 caccataggc tcaaggaggc aatgaggata gcctcaatgg ctcgaaatga ctacgcgttg 840 cctttctttg tttatgctcg accattctat gcctatacct tcgaaccatt gactcaggaa 900 gaccttgtga ctacggtcgg agagaccgcg gacatgggcg ccgctgggat cgtattttgg 960 gggagtatgc agtacgcaag cacggttgaa tcttgcggca aagtcaagga ctacatgaat 1020 ggcccactgg ggcgttacat tgtgaatgtt acaactgccg ccaaaatttg ctcacgattc 1080 ttgtgcaaac gtcatggtag gtgtgtaaga aagcactcag actccaatgc attccttcac 1140 ctatttcccg attcgtttcg cataatggtg cacgggaatg caaccgagaa gaaagttata 1200 gtaaagggga agctggaatt aaagaatctt atattcttac gtaataactt tatgtgccag 1260 tgttatcaag gctggaaagg tctatattgc gagaagcatt cgataaagga aattcggaaa 1320 atctaa 1326 <210> 20 <211> 1537 <212> DNA <213> Bothrops atrox <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal and a C-terminal anchoring signal <400> 20 atgcaattta gcacagtcgc atcagtagcc ttcgttgcct tggccaactt cgtggcagca 60 ccgatgtatc cgaacgaacc gttcttagtc ttttggaacg cgcctacaac tcagtgtaga 120 cttcgatata aggttgacct tgatctgaag acattccata tcgtgacaaa tgctaatgac 180 tcgctgtcag gatcggctgt cacgattttc tatcccacgc acttaggggt ttacccacat 240 attgatgaca gggggcactt cttcaatggc atcatacccc aaaatgaatc cctggtaaag 300 catttaaaca aatctaaatc agatattaat cgaatgattc ccttaagaac attccacggg 360 ctgggagtca tagactggga aaactggcgg ccacagtggg ataggaattg gggaagtaag 420 aacgtttata ggaatagatc aatccaattc gcgcgtgatc tccacccaga gcttagtgag 480 gacaagatta aacgcttggc aaaacaggaa ttcgagaaag ctgcaaagag ttttatgagg 540 gatacactat tattagccga ggagatgcga ccagacggct actggggata ctatctgtac 600 cccgattgtc acaattacaa ttataagact aagccagatc agtacacagg agagtgccct 660 gacatcgaga tgtcacgtaa caatcaactc ttgtggcttt ggcgggatag cactgccctt 720 ttccccaata tctacttaga gactatacta agaagctctg acaatgccct gaagtttgtg 780 caccataggc tcaaggaggc aatgaggata gcctcaatgg ctcgaaatga ctacgcgttg 840 cctttctttg tttatgctcg accattctat gcctatacct tcgaaccatt gactcaggaa 900 gaccttgtga ctacggtcgg agagaccgcg gacatgggcg ccgctgggat cgtattttgg 960 gggagtatgc agtacgcaag cacggttgaa tcttgcggca aagtcaagga ctacatgaat 1020 ggcccactgg ggcgttacat tgtgaatgtt acaactgccg ccaaaatttg ctcacgattc 1080 ttgtgcaaac gtcatggtag gtgtgtaaga aagcactcag actccaatgc attccttcac 1140 ctatttcccg attcgtttcg cataatggtg cacgggaatg caaccgagaa gaaagttata 1200 gtaaagggga agctggaatt aaagaatctt atattcttac gtaataactt tatgtgccag 1260 tgttatcaag gctggaaagg tctatattgc gagaagcatt cgataaagga aattcggaaa 1320 atcggatccg ccatttctca aatcactgac ggtcaaatcc aagctactac cactgctacc 1380 accgaagcta ccaccactgc tgccccatct tccaccgttg aaactgtttc tccatccagc 1440 accgaaacta tctctcaaca aactgaaaat ggtgctgcta aggccgctgt cggtatgggt 1500 gccggtgctc tagctgctgc tgctatgttg ttataaa 1537 <210> 21 <211> 1197 <212> DNA <213> Cupiennius salei <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal <400> 21 atgcaattca gcactgttgc atcagttgca tttgtcgccc tggcgaattt tgtagccgcg 60 ttcaagattt actggaacgt cccaactttt cagtgcacgc ataactacaa aatcgattat 120 gtcaaattgt tgtccactta cgggatacag gtcaatgatg gcggtaagtt tcaaggaaac 180 caagtgacta tcttttatga aacccagttg ggtttgtatc cacgaatcct aaaatctggt 240 aaaatggaaa acggcggaat ccctcaacgc ggtaactttg agaaacacct agaaaaggca 300 agcacggacc tccagaaagt gatcccttgg aaagagttta gcggattagg tgtgatagat 360 tgggaggctt ggagacccac atgggaattt aactgggaac cgttgaggat atatcaaacc 420 gaatcaatta agagagctaa agaactacac cctaccgcaa acgattccgc agtaaaagaa 480 attgcagagc ggcaatggga agattcagcc aagttataca tgttagaaac actgcggctg 540 gcaaagaaac ttcgacctca agcgccttgg tgttactact tatttcctga ttgctataat 600 tacgtcggaa agaaaccaaa agatttccaa tgtagtgcct cgatacgtaa aggtaacgat 660 aagctaagct ggttgtggaa agattctacg gcattgtgtc catcgatata cgtatatgaa 720 tcacaattag acaggtattc ttttgaacaa aggacatggc gcgacaatga gaaacttcgg 780 gaagcgttgc gtgtagccac gagaacctct aaaatatacc catacgttaa ctatttcgat 840 aaggagctta taccggagca agaagtatgg agaatgcttg cgcaggcagc tgctgtcggt 900 ggcagtggtg cggtaatttg gggctcatct gctgcagttg catctgaaga gttatgtaaa 960 tctttaaaac agtatattat tgaaacgctt gggccggcgg cagagaaggt ggcttggcgt 1020 agtgacttat gcagcaaaga aatttgtaat aatcagggtc gctgcacatt cccggacgat 1080 gattatgcaa acgcatggaa attatttaca gatgatactg ttaagtttta tgctggtaat 1140 attacatgta ggtgctccga gaattattct ggtcgtttct gcgaaaagaa gaattaa 1197 <210> 22 <211> 1407 <212> DNA <213> Cupiennius salei <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal and a C-terminal anchoring signal <400> 22 atgcaattca gcactgttgc atcagttgca tttgtcgccc tggcgaattt tgtagccgcg 60 ttcaagattt actggaacgt cccaactttt cagtgcacgc ataactacaa aatcgattat 120 gtcaaattgt tgtccactta cgggatacag gtcaatgatg gcggtaagtt tcaaggaaac 180 caagtgacta tcttttatga aacccagttg ggtttgtatc cacgaatcct aaaatctggt 240 aaaatggaaa acggcggaat ccctcaacgc ggtaactttg agaaacacct agaaaaggca 300 agcacggacc tccagaaagt gatcccttgg aaagagttta gcggattagg tgtgatagat 360 tgggaggctt ggagacccac atgggaattt aactgggaac cgttgaggat atatcaaacc 420 gaatcaatta agagagctaa agaactacac cctaccgcaa acgattccgc agtaaaagaa 480 attgcagagc ggcaatggga agattcagcc aagttataca tgttagaaac actgcggctg 540 gcaaagaaac ttcgacctca agcgccttgg tgttactact tatttcctga ttgctataat 600 tacgtcggaa agaaaccaaa agatttccaa tgtagtgcct cgatacgtaa aggtaacgat 660 aagctaagct ggttgtggaa agattctacg gcattgtgtc catcgatata cgtatatgaa 720 tcacaattag acaggtattc ttttgaacaa aggacatggc gcgacaatga gaaacttcgg 780 gaagcgttgc gtgtagccac gagaacctct aaaatatacc catacgttaa ctatttcgat 840 aaggagctta taccggagca agaagtatgg agaatgcttg cgcaggcagc tgctgtcggt 900 ggcagtggtg cggtaatttg gggctcatct gctgcagttg catctgaaga gttatgtaaa 960 tctttaaaac agtatattat tgaaacgctt gggccggcgg cagagaaggt ggcttggcgt 1020 agtgacttat gcagcaaaga aatttgtaat aatcagggtc gctgcacatt cccggacgat 1080 gattatgcaa acgcatggaa attatttaca gatgatactg ttaagtttta tgctggtaat 1140 attacatgta ggtgctccga gaattattct ggtcgtttct gcgaaaagaa gaatggatcc 1200 gccatttctc aaatcactga cggtcaaatc caagctacta ccactgctac caccgaagct 1260 accaccactg ctgccccatc ttccaccgtt gaaactgttt ctccatccag caccgaaact 1320 atctctcaac aaactgaaaa tggtgctgct aaggccgctg tcggtatggg tgccggtgct 1380 ctagctgctg ctgctatgtt gttataa 1407 <210> 23 <211> 1530 <212> DNA <213> Hirudo nipponia <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal <400> 23 atgcaattct ctactgtcgc ttccgttgct ttcgtcgctt tggctaactt tgttgccgct 60 atgaaggaga tagcagttac catcgatgat aagaatgtta tagcgtctgt cagtgaaagc 120 ttccatggtg tggctttcga cgcaagccta ttctcaccta aagggctatg gtcgtttgtt 180 gacattactt cacccaagtt attcaagctt ctggagggac tctcccctgg ttatttccgt 240 gtcggcggca catttgcgaa ctggttattc ttcgacctcg atgaaaacaa caagtggaag 300 gattactggg cttttaagga caagactcca gagaccgcca cgattacccg acggtggctc 360 ttcaggaagc aaaacaacct caagaaggaa acctttgatg atttggtaaa gcttacaaag 420 gggtccaaga tgcggctgct atttgatcta aacgcagagg tccgtacagg atatgagata 480 ggcaagaaga tgacatcaac ttgggactca agcgaggcag aaaagttgtt caaatattgc 540 gttagcaagg gatatggaga taatatcgat tgggagttag gcaacgaacc tgatcacacg 600 tcagcacaca atttgacaga gaagcaagta ggtgaagatt ttaaggccct acacaaggtg 660 ttggaaaagt atccaacact taataaaggt agcttggttg gtccagacgt tgggtggatg 720 ggagtgtcgt acgtcaaggg tctggctgac ggggctggag atcatgtgac cgcttttact 780 ctacatcagt attatttcga tggaaatacg agtgatgtta gtacctactt ggatgcgacg 840 tactttaaga agttgcaaca gttattcgat aaggtaaaag acgtactcaa gaattctcca 900 cataaggaca aacccttgtg gctaggggaa acctcttccg gctacaacag tgggactaaa 960 gatgtatccg atagatacgt gtcggggttc ttgacgctgg acaagttggg gctttcggcg 1020 gcaaataacg tcaaggtggt catcagacaa acgatttata atggttacta tggtttgctt 1080 gacaagaata ctttggagcc caatccggac tactggctga tgcacgtcca caattcctta 1140 gttggtaata ccgttttcaa ggtcgatgtc agcgatccca caaacaaagc ccgtgtttac 1200 gctcagtgta cgaagaccaa tagtaaacat acccagtcac gttattataa ggggtcccta 1260 accattttcg cgttgaatgt aggtgatgaa gatgttacac tcaagattga tcaatacagt 1320 ggtaagaaaa tatactcata tattctgacg cctgaaggcg gccagttaac ctctcaaaag 1380 gtactgctta acggtaaaga attgaagtta gtgtcagatc aacttccaga acttaacgcg 1440 gacgaaagta aaacatcctt cacattgtct cccaaaactt ttggattctt tgtggtctcg 1500 gatgccaacg ttgaagcttg caagaaataa 1530 <210> 24 <211> 1740 <212> DNA <213> Hirudo nipponia <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal and a C-terminal anchoring signal <400> 24 atgcaattct ctactgtcgc ttccgttgct ttcgtcgctt tggctaactt tgttgccgct 60 atgaaggaga tagcagttac catcgatgat aagaatgtta tagcgtctgt cagtgaaagc 120 ttccatggtg tggctttcga cgcaagccta ttctcaccta aagggctatg gtcgtttgtt 180 gacattactt cacccaagtt attcaagctt ctggagggac tctcccctgg ttatttccgt 240 gtcggcggca catttgcgaa ctggttattc ttcgacctcg atgaaaacaa caagtggaag 300 gattactggg cttttaagga caagactcca gagaccgcca cgattacccg acggtggctc 360 ttcaggaagc aaaacaacct caagaaggaa acctttgatg atttggtaaa gcttacaaag 420 gggtccaaga tgcggctgct atttgatcta aacgcagagg tccgtacagg atatgagata 480 ggcaagaaga tgacatcaac ttgggactca agcgaggcag aaaagttgtt caaatattgc 540 gttagcaagg gatatggaga taatatcgat tgggagttag gcaacgaacc tgatcacacg 600 tcagcacaca atttgacaga gaagcaagta ggtgaagatt ttaaggccct acacaaggtg 660 ttggaaaagt atccaacact taataaaggt agcttggttg gtccagacgt tgggtggatg 720 ggagtgtcgt acgtcaaggg tctggctgac ggggctggag atcatgtgac cgcttttact 780 ctacatcagt attatttcga tggaaatacg agtgatgtta gtacctactt ggatgcgacg 840 tactttaaga agttgcaaca gttattcgat aaggtaaaag acgtactcaa gaattctcca 900 cataaggaca aacccttgtg gctaggggaa acctcttccg gctacaacag tgggactaaa 960 gatgtatccg atagatacgt gtcggggttc ttgacgctgg acaagttggg gctttcggcg 1020 gcaaataacg tcaaggtggt catcagacaa acgatttata atggttacta tggtttgctt 1080 gacaagaata ctttggagcc caatccggac tactggctga tgcacgtcca caattcctta 1140 gttggtaata ccgttttcaa ggtcgatgtc agcgatccca caaacaaagc ccgtgtttac 1200 gctcagtgta cgaagaccaa tagtaaacat acccagtcac gttattataa ggggtcccta 1260 accattttcg cgttgaatgt aggtgatgaa gatgttacac tcaagattga tcaatacagt 1320 ggtaagaaaa tatactcata tattctgacg cctgaaggcg gccagttaac ctctcaaaag 1380 gtactgctta acggtaaaga attgaagtta gtgtcagatc aacttccaga acttaacgcg 1440 gacgaaagta aaacatcctt cacattgtct cccaaaactt ttggattctt tgtggtctcg 1500 gatgccaacg ttgaagcttg caagaaagga tccgccattt ctcaaatcac tgacggtcaa 1560 atccaagcta ctaccactgc taccaccgaa gctaccacca ctgctgcccc atcttccacc 1620 gttgaaactg tttctccatc cagcaccgaa actatctctc aacaaactga aaatggtgct 1680 gctaaggccg ctgtcggtat gggtgccggt gctctagctg ctgctgctat gttgttataa 1740 <210> 25 <211> 1206 <212> DNA <213> Loxosceles intermedia <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal <400> 25 atgcagtttt caaccgttgc gagcgtcgct tttgtggctc ttgctaattt cgttgccgcc 60 ttcgatgtct tctggaatgt tccgtcgcag caatgcaaga aatatggtat gaaattcgtt 120 ccgctcttag agcaatactc tatcctagtc aacaaagaag acaatttcaa gggcgacaaa 180 ataacgatct tttatgagtc acagctcggg ctttacccac atattggtgc aaacgacgag 240 tcgtttaatg gcgggatacc acaattaggt gacctgaaag cacacttaga aaagtcagcg 300 gttgatatac gacgtgatat tttggataag tcggcgactg gtctaagaat tatagactgg 360 gaagcatgga gaccaatatg ggaattcaac tggtctagcc tacgaaagta ccaagataaa 420 atgaagaaag tcgtccgcca gtttaacccg actgctcatg aatccacagt ggccaaacta 480 gcacataatg agtgggaaaa tagtagtaag tcttggatgc tttctacatt gcagttgggt 540 aagcaacttc gtccaaactc tgtatggtgc tattatttat ttcccgactg ttataactat 600 gatggcaact cagtccaaga atttcagtgt tctgaagcta tccgtaaggg gaacgatagg 660 ttgaaatggt tgtgggaaga atcgacagct gtatgcccat ctatctacat aaaagaaggc 720 caactgacca attatacctt gcaaaagaga atctggttta ccaatgggag attacaggaa 780 gccttgagag tagctcaacc taaagcgcgt atttatccat acataaatta ctccatcaaa 840 cccggaatga tggtgcctga agttgagttt tggcggttaa tcgctcagat agcctcgctg 900 ggtatggatg gagcagtgat ttggggatca agtgcgagtg taggcagtaa gaatcattgt 960 gcgcaattaa tgaagtacat tgcagacgta ttgggtcctg caactttgcg cataaaagaa 1020 aatgtagcac ggtgcagtaa acaggcgtgc tctggtaggg gtagatgtac ctggcctaaa 1080 gatacctctg ttattgcttg gaagttcctc gttgaaaagg aagactatga cttttatctg 1140 ggtgatatag agtgtaaatg tgttgaaggc tacgaaggta ggtactgtga acaaaagact 1200 aagtaa 1206 <210> 26 <211> 1416 <212> DNA <213> Loxosceles intermedia <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal and a C-terminal anchoring signal <400> 26 atgcagtttt caaccgttgc gagcgtcgct tttgtggctc ttgctaattt cgttgccgcc 60 ttcgatgtct tctggaatgt tccgtcgcag caatgcaaga aatatggtat gaaattcgtt 120 ccgctcttag agcaatactc tatcctagtc aacaaagaag acaatttcaa gggcgacaaa 180 ataacgatct tttatgagtc acagctcggg ctttacccac atattggtgc aaacgacgag 240 tcgtttaatg gcgggatacc acaattaggt gacctgaaag cacacttaga aaagtcagcg 300 gttgatatac gacgtgatat tttggataag tcggcgactg gtctaagaat tatagactgg 360 gaagcatgga gaccaatatg ggaattcaac tggtctagcc tacgaaagta ccaagataaa 420 atgaagaaag tcgtccgcca gtttaacccg actgctcatg aatccacagt ggccaaacta 480 gcacataatg agtgggaaaa tagtagtaag tcttggatgc tttctacatt gcagttgggt 540 aagcaacttc gtccaaactc tgtatggtgc tattatttat ttcccgactg ttataactat 600 gatggcaact cagtccaaga atttcagtgt tctgaagcta tccgtaaggg gaacgatagg 660 ttgaaatggt tgtgggaaga atcgacagct gtatgcccat ctatctacat aaaagaaggc 720 caactgacca attatacctt gcaaaagaga atctggttta ccaatgggag attacaggaa 780 gccttgagag tagctcaacc taaagcgcgt atttatccat acataaatta ctccatcaaa 840 cccggaatga tggtgcctga agttgagttt tggcggttaa tcgctcagat agcctcgctg 900 ggtatggatg gagcagtgat ttggggatca agtgcgagtg taggcagtaa gaatcattgt 960 gcgcaattaa tgaagtacat tgcagacgta ttgggtcctg caactttgcg cataaaagaa 1020 aatgtagcac ggtgcagtaa acaggcgtgc tctggtaggg gtagatgtac ctggcctaaa 1080 gatacctctg ttattgcttg gaagttcctc gttgaaaagg aagactatga cttttatctg 1140 ggtgatatag agtgtaaatg tgttgaaggc tacgaaggta ggtactgtga acaaaagact 1200 aagggatccg ccatttctca aatcactgac ggtcaaatcc aagctactac cactgctacc 1260 accgaagcta ccaccactgc tgccccatct tccaccgttg aaactgtttc tccatccagc 1320 accgaaacta tctctcaaca aactgaaaat ggtgctgcta aggccgctgt cggtatgggt 1380 gccggtgctc tagctgctgc tgctatgttg ttataa 1416 <210> 27 <211> 1218 <212> DNA <213> Tityus serrulatus <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal <400> 27 atgcaattct ctactgtcgc ttccgttgct ttcgtcgctt tggctaactt tgttgccgct 60 gctgatttta aagtttactg ggaagtgcct tccttccttt gttctaaacg ttttaaaatt 120 aatgtaacgg aagttttaac aagtcacgag attcttgtca atcagggtga gagtttcaac 180 ggtgacaaga tagtaatctt ttacgaaaac caattgggga agtacccgca tattgactca 240 aacaatgtgg agatcaatgg aggaatactt caagtagccg atttggcgaa gcatttgaaa 300 gtagccaagg ataatatcac taaattcgtc ccgaatccta atttcaacgg tgtcggagtg 360 atcgactggg aagcttggcg gccatcatgg gaatttaact ggggtaagtt aaaagtatat 420 aaagaaaaga gcattgactt ggtcaagtcg aaacatccgg agtggccctc cgacagggtt 480 gaaaaggttg ctaaagagga gtgggaggag agtgccaaag aatggatggt gaagaccctg 540 aagttagcac aggaaatgcg accgaacgca gtttggtgct attatctatt ccctgactgc 600 tacaattatt tcggtaagga tcaaccctct caattcagct gctcgtctcg aattcagaag 660 gaaaattctc gtctttcttg gctctggaat caatcaacag ccatttgcct aagcatttat 720 atccaggaat cccatgttac caaatataat atgtcccagc ggacatggtg gatcgatgcg 780 agattaagag aagcaattcg agtcagcgaa cacagaccaa acatacccat ctacccttac 840 attaattata ttctacctgg aactaatcaa actgtaccag caatggactt taaaaggaca 900 ctgggtcaaa tagctagcct cggcctagat ggtgctttgt tatggggatc tagctatcat 960 gttttaacag aatctcaatg caaaatcact tctgattatg tgaaatcagt gattgctcct 1020 accgtggcta ctgtcgttct caatacaaac agatgctcac agataatttg taagggtcgc 1080 ggcaactgtg tttggcctga agaaccattt agttcttgga aatacttagt tgaccccaaa 1140 atgccagtgt tcaagccaac caacatccac tgtaaatgta aaggttacct aggtagatac 1200 tgtgagatcc caaagtaa 1218 <210> 28 <211> 1428 <212> DNA <213> Tityus serrulatus <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal and a C-terminal anchoring signal <400> 28 atgcaattct ctactgtcgc ttccgttgct ttcgtcgctt tggctaactt tgttgccgct 60 gctgatttta aagtttactg ggaagtgcct tccttccttt gttctaaacg ttttaaaatt 120 aatgtaacgg aagttttaac aagtcacgag attcttgtca atcagggtga gagtttcaac 180 ggtgacaaga tagtaatctt ttacgaaaac caattgggga agtacccgca tattgactca 240 aacaatgtgg agatcaatgg aggaatactt caagtagccg atttggcgaa gcatttgaaa 300 gtagccaagg ataatatcac taaattcgtc ccgaatccta atttcaacgg tgtcggagtg 360 atcgactggg aagcttggcg gccatcatgg gaatttaact ggggtaagtt aaaagtatat 420 aaagaaaaga gcattgactt ggtcaagtcg aaacatccgg agtggccctc cgacagggtt 480 gaaaaggttg ctaaagagga gtgggaggag agtgccaaag aatggatggt gaagaccctg 540 aagttagcac aggaaatgcg accgaacgca gtttggtgct attatctatt ccctgactgc 600 tacaattatt tcggtaagga tcaaccctct caattcagct gctcgtctcg aattcagaag 660 gaaaattctc gtctttcttg gctctggaat caatcaacag ccatttgcct aagcatttat 720 atccaggaat cccatgttac caaatataat atgtcccagc ggacatggtg gatcgatgcg 780 agattaagag aagcaattcg agtcagcgaa cacagaccaa acatacccat ctacccttac 840 attaattata ttctacctgg aactaatcaa actgtaccag caatggactt taaaaggaca 900 ctgggtcaaa tagctagcct cggcctagat ggtgctttgt tatggggatc tagctatcat 960 gttttaacag aatctcaatg caaaatcact tctgattatg tgaaatcagt gattgctcct 1020 accgtggcta ctgtcgttct caatacaaac agatgctcac agataatttg taagggtcgc 1080 ggcaactgtg tttggcctga agaaccattt agttcttgga aatacttagt tgaccccaaa 1140 atgccagtgt tcaagccaac caacatccac tgtaaatgta aaggttacct aggtagatac 1200 tgtgagatcc caaagggatc cgccatttct caaatcactg acggtcaaat ccaagctact 1260 accactgcta ccaccgaagc taccaccact gctgccccat cttccaccgt tgaaactgtt 1320 tctccatcca gcaccgaaac tatctctcaa caaactgaaa atggtgctgc taaggccgct 1380 gtcggtatgg gtgccggtgc tctagctgct gctgctatgt tgttataa 1428 <210> 29 <211> 1083 <212> DNA <213> Vespa magnifica <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal <400> 29 atgcaatttt ctacagtggc aagtgttgca ttcgttgcac tagccaactt tgtggcggca 60 gatagctgtg ggtcaaactg cgaaaagagt gagagaccga aaagggtctt caacatttac 120 tggaacgtac ctacattcat gtgtcaccag tacggactat actttgacga ggtcacgaat 180 tttaatataa agcacaacag caaagacaat tttcaagggg acaagatcgc gatcttttat 240 gaccccgggg agtttcccgc tctgctgcca ctaaactatg gtaagtacaa gatcaggaat 300 ggtggtgttc cacaagaggg taacatcacc atccatctgc agagattcat agagtaccta 360 gataagacct atccgaaccg taacttttca ggcatcggtg tgatcgattt cgagaggtgg 420 agaccaattt tcagacagaa ttggggtaat atgaagattt acaagaactt ctccatcgat 480 cttgtgcgta aagagcatcc tttctggaat aagaaaatga tcgagttgga agcttctaaa 540 agattcgaga aatacgcccg tctgttcatg gaagaaacat taaagttggc taagaaaact 600 agaaaacagg ccgattgggg ctactacggt tacccctatt gcttcaacat gtctcctact 660 aatttcgttc ctgactgcga tgtcacagct agggatgaga acaacgagat gtcttggttg 720 tttaacaacc agaatgtcct attaccaagt gtatacatta ggagagagct aactcctgac 780 cagaggattg ggcttgtaca ggggagagtg aaggaagctg tgagaatttc aaataaactg 840 aagcactcac ctaaagtctt cagctattgg tggtatgttt accaagacga gaccaacacc 900 ttcttaacgg agaccgacgt caagaagacg tttcaggaga ttgtgatcaa cggtggagat 960 gggattataa tctggggttc gtcctctgat gtaaacagct tgtccaagtg tacgaggtta 1020 agggagtacc tattgacagt cttgggacca attgctgtta acgtgactga agcagtaaac 1080 taa 1083 <210> 30 <211> 1293 <212> DNA <213> Vespa magnifica <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Reencoded nucleic acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal and a C-terminal anchoring signal <400> 30 atgcaatttt ctacagtggc aagtgttgca ttcgttgcac tagccaactt tgtggcggca 60 gatagctgtg ggtcaaactg cgaaaagagt gagagaccga aaagggtctt caacatttac 120 tggaacgtac ctacattcat gtgtcaccag tacggactat actttgacga ggtcacgaat 180 tttaatataa agcacaacag caaagacaat tttcaagggg acaagatcgc gatcttttat 240 gaccccgggg agtttcccgc tctgctgcca ctaaactatg gtaagtacaa gatcaggaat 300 ggtggtgttc cacaagaggg taacatcacc atccatctgc agagattcat agagtaccta 360 gataagacct atccgaaccg taacttttca ggcatcggtg tgatcgattt cgagaggtgg 420 agaccaattt tcagacagaa ttggggtaat atgaagattt acaagaactt ctccatcgat 480 cttgtgcgta aagagcatcc tttctggaat aagaaaatga tcgagttgga agcttctaaa 540 agattcgaga aatacgcccg tctgttcatg gaagaaacat taaagttggc taagaaaact 600 agaaaacagg ccgattgggg ctactacggt tacccctatt gcttcaacat gtctcctact 660 aatttcgttc ctgactgcga tgtcacagct agggatgaga acaacgagat gtcttggttg 720 tttaacaacc agaatgtcct attaccaagt gtatacatta ggagagagct aactcctgac 780 cagaggattg ggcttgtaca ggggagagtg aaggaagctg tgagaatttc aaataaactg 840 aagcactcac ctaaagtctt cagctattgg tggtatgttt accaagacga gaccaacacc 900 ttcttaacgg agaccgacgt caagaagacg tttcaggaga ttgtgatcaa cggtggagat 960 gggattataa tctggggttc gtcctctgat gtaaacagct tgtccaagtg tacgaggtta 1020 agggagtacc tattgacagt cttgggacca attgctgtta acgtgactga agcagtaaac 1080 ggatccgcca tttctcaaat cactgacggt caaatccaag ctactaccac tgctaccacc 1140 gaagctacca ccactgctgc cccatcttcc accgttgaaa ctgtttctcc atccagcacc 1200 gaaactatct ctcaacaaac tgaaaatggt gctgctaagg ccgctgtcgg tatgggtgcc 1260 ggtgctctag ctgctgctgc tatgttgtta taa 1293 <210> 31 <211> 441 <212> PRT <213> Bothrops atrox <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal <400> 31 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Pro Met Tyr Pro Asn Glu Pro Phe Leu Val Phe Trp 20 25 30 Asn Ala Pro Thr Thr Gln Cys Arg Leu Arg Tyr Lys Val Asp Leu Asp 35 40 45 Leu Lys Thr Phe His Ile Val Thr Asn Ala Asn Asp Ser Leu Ser Gly 50 55 60 Ser Ala Val Thr Ile Phe Tyr Pro Thr His Leu Gly Val Tyr Pro His 65 70 75 80 Ile Asp Asp Arg Gly His Phe Phe Asn Gly Ile Ile Pro Gln Asn Glu 85 90 95 Ser Leu Val Lys His Leu Asn Lys Ser Lys Ser Asp Ile Asn Arg Met 100 105 110 Ile Pro Leu Arg Thr Phe His Gly Leu Gly Val Ile Asp Trp Glu Asn 115 120 125 Trp Arg Pro Gln Trp Asp Arg Asn Trp Gly Ser Lys Asn Val Tyr Arg 130 135 140 Asn Arg Ser Ile Gln Phe Ala Arg Asp Leu His Pro Glu Leu Ser Glu 145 150 155 160 Asp Lys Ile Lys Arg Leu Ala Lys Gln Glu Phe Glu Lys Ala Ala Lys 165 170 175 Ser Phe Met Arg Asp Thr Leu Leu Leu Ala Glu Glu Met Arg Pro Asp 180 185 190 Gly Tyr Trp Gly Tyr Tyr Leu Tyr Pro Asp Cys His Asn Tyr Asn Tyr 195 200 205 Lys Thr Lys Pro Asp Gln Tyr Thr Gly Glu Cys Pro Asp Ile Glu Met 210 215 220 Ser Arg Asn Asn Gln Leu Leu Trp Leu Trp Arg Asp Ser Thr Ala Leu 225 230 235 240 Phe Pro Asn Ile Tyr Leu Glu Thr Ile Leu Arg Ser Ser Asp Asn Ala 245 250 255 Leu Lys Phe Val His His Arg Leu Lys Glu Ala Met Arg Ile Ala Ser 260 265 270 Met Ala Arg Asn Asp Tyr Ala Leu Pro Phe Phe Val Tyr Ala Arg Pro 275 280 285 Phe Tyr Ala Tyr Thr Phe Glu Pro Leu Thr Gln Glu Asp Leu Val Thr 290 295 300 Thr Val Gly Glu Thr Ala Asp Met Gly Ala Ala Gly Ile Val Phe Trp 305 310 315 320 Gly Ser Met Gln Tyr Ala Ser Thr Val Glu Ser Cys Gly Lys Val Lys 325 330 335 Asp Tyr Met Asn Gly Pro Leu Gly Arg Tyr Ile Val Asn Val Thr Thr 340 345 350 Ala Ala Lys Ile Cys Ser Arg Phe Leu Cys Lys Arg His Gly Arg Cys 355 360 365 Val Arg Lys His Ser Asp Ser Asn Ala Phe Leu His Leu Phe Pro Asp 370 375 380 Ser Phe Arg Ile Met Val His Gly Asn Ala Thr Glu Lys Lys Val Ile 385 390 395 400 Val Lys Gly Lys Leu Glu Leu Lys Asn Leu Ile Phe Leu Arg Asn Asn 405 410 415 Phe Met Cys Gln Cys Tyr Gln Gly Trp Lys Gly Leu Tyr Cys Glu Lys 420 425 430 His Ser Ile Lys Glu Ile Arg Lys Ile 435 440 <210> 32 <211> 511 <212> PRT <213> Bothrops atrox <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Bothrops atrox with a N-terminal secretion signal and a C-terminal anchoring signal <400> 32 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Pro Met Tyr Pro Asn Glu Pro Phe Leu Val Phe Trp 20 25 30 Asn Ala Pro Thr Thr Gln Cys Arg Leu Arg Tyr Lys Val Asp Leu Asp 35 40 45 Leu Lys Thr Phe His Ile Val Thr Asn Ala Asn Asp Ser Leu Ser Gly 50 55 60 Ser Ala Val Thr Ile Phe Tyr Pro Thr His Leu Gly Val Tyr Pro His 65 70 75 80 Ile Asp Asp Arg Gly His Phe Phe Asn Gly Ile Ile Pro Gln Asn Glu 85 90 95 Ser Leu Val Lys His Leu Asn Lys Ser Lys Ser Asp Ile Asn Arg Met 100 105 110 Ile Pro Leu Arg Thr Phe His Gly Leu Gly Val Ile Asp Trp Glu Asn 115 120 125 Trp Arg Pro Gln Trp Asp Arg Asn Trp Gly Ser Lys Asn Val Tyr Arg 130 135 140 Asn Arg Ser Ile Gln Phe Ala Arg Asp Leu His Pro Glu Leu Ser Glu 145 150 155 160 Asp Lys Ile Lys Arg Leu Ala Lys Gln Glu Phe Glu Lys Ala Ala Lys 165 170 175 Ser Phe Met Arg Asp Thr Leu Leu Leu Ala Glu Glu Met Arg Pro Asp 180 185 190 Gly Tyr Trp Gly Tyr Tyr Leu Tyr Pro Asp Cys His Asn Tyr Asn Tyr 195 200 205 Lys Thr Lys Pro Asp Gln Tyr Thr Gly Glu Cys Pro Asp Ile Glu Met 210 215 220 Ser Arg Asn Asn Gln Leu Leu Trp Leu Trp Arg Asp Ser Thr Ala Leu 225 230 235 240 Phe Pro Asn Ile Tyr Leu Glu Thr Ile Leu Arg Ser Ser Asp Asn Ala 245 250 255 Leu Lys Phe Val His His Arg Leu Lys Glu Ala Met Arg Ile Ala Ser 260 265 270 Met Ala Arg Asn Asp Tyr Ala Leu Pro Phe Phe Val Tyr Ala Arg Pro 275 280 285 Phe Tyr Ala Tyr Thr Phe Glu Pro Leu Thr Gln Glu Asp Leu Val Thr 290 295 300 Thr Val Gly Glu Thr Ala Asp Met Gly Ala Ala Gly Ile Val Phe Trp 305 310 315 320 Gly Ser Met Gln Tyr Ala Ser Thr Val Glu Ser Cys Gly Lys Val Lys 325 330 335 Asp Tyr Met Asn Gly Pro Leu Gly Arg Tyr Ile Val Asn Val Thr Thr 340 345 350 Ala Ala Lys Ile Cys Ser Arg Phe Leu Cys Lys Arg His Gly Arg Cys 355 360 365 Val Arg Lys His Ser Asp Ser Asn Ala Phe Leu His Leu Phe Pro Asp 370 375 380 Ser Phe Arg Ile Met Val His Gly Asn Ala Thr Glu Lys Lys Val Ile 385 390 395 400 Val Lys Gly Lys Leu Glu Leu Lys Asn Leu Ile Phe Leu Arg Asn Asn 405 410 415 Phe Met Cys Gln Cys Tyr Gln Gly Trp Lys Gly Leu Tyr Cys Glu Lys 420 425 430 His Ser Ile Lys Glu Ile Arg Lys Ile Gly Ser Ala Ile Ser Gln Ile 435 440 445 Thr Asp Gly Gln Ile Gln Ala Thr Thr Thr Ala Thr Thr Glu Ala Thr 450 455 460 Thr Thr Ala Ala Pro Ser Ser Thr Val Glu Thr Val Ser Pro Ser Ser 465 470 475 480 Thr Glu Thr Ile Ser Gln Gln Thr Glu Asn Gly Ala Ala Lys Ala Ala 485 490 495 Val Gly Met Gly Ala Gly Ala Leu Ala Ala Ala Ala Met Leu Leu 500 505 510 <210> 33 <211> 398 <212> PRT <213> Cupiennius salei <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal <400> 33 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Phe Lys Ile Tyr Trp Asn Val Pro Thr Phe Gln Cys 20 25 30 Thr His Asn Tyr Lys Ile Asp Tyr Val Lys Leu Leu Ser Thr Tyr Gly 35 40 45 Ile Gln Val Asn Asp Gly Gly Lys Phe Gln Gly Asn Gln Val Thr Ile 50 55 60 Phe Tyr Glu Thr Gln Leu Gly Leu Tyr Pro Arg Ile Leu Lys Ser Gly 65 70 75 80 Lys Met Glu Asn Gly Gly Ile Pro Gln Arg Gly Asn Phe Glu Lys His 85 90 95 Leu Glu Lys Ala Ser Thr Asp Leu Gln Lys Val Ile Pro Trp Lys Glu 100 105 110 Phe Ser Gly Leu Gly Val Ile Asp Trp Glu Ala Trp Arg Pro Thr Trp 115 120 125 Glu Phe Asn Trp Glu Pro Leu Arg Ile Tyr Gln Thr Glu Ser Ile Lys 130 135 140 Arg Ala Lys Glu Leu His Pro Thr Ala Asn Asp Ser Ala Val Lys Glu 145 150 155 160 Ile Ala Glu Arg Gln Trp Glu Asp Ser Ala Lys Leu Tyr Met Leu Glu 165 170 175 Thr Leu Arg Leu Ala Lys Lys Leu Arg Pro Gln Ala Pro Trp Cys Tyr 180 185 190 Tyr Leu Phe Pro Asp Cys Tyr Asn Tyr Val Gly Lys Lys Pro Lys Asp 195 200 205 Phe Gln Cys Ser Ala Ser Ile Arg Lys Gly Asn Asp Lys Leu Ser Trp 210 215 220 Leu Trp Lys Asp Ser Thr Ala Leu Cys Pro Ser Ile Tyr Val Tyr Glu 225 230 235 240 Ser Gln Leu Asp Arg Tyr Ser Phe Glu Gln Arg Thr Trp Arg Asp Asn 245 250 255 Glu Lys Leu Arg Glu Ala Leu Arg Val Ala Thr Arg Thr Ser Lys Ile 260 265 270 Tyr Pro Tyr Val Asn Tyr Phe Asp Lys Glu Leu Ile Pro Glu Gln Glu 275 280 285 Val Trp Arg Met Leu Ala Gln Ala Ala Ala Val Gly Gly Ser Gly Ala 290 295 300 Val Ile Trp Gly Ser Ser Ala Ala Val Ala Ser Glu Glu Leu Cys Lys 305 310 315 320 Ser Leu Lys Gln Tyr Ile Ile Glu Thr Leu Gly Pro Ala Ala Glu Lys 325 330 335 Val Ala Trp Arg Ser Asp Leu Cys Ser Lys Glu Ile Cys Asn Asn Gln 340 345 350 Gly Arg Cys Thr Phe Pro Asp Asp Asp Tyr Ala Asn Ala Trp Lys Leu 355 360 365 Phe Thr Asp Asp Thr Val Lys Phe Tyr Ala Gly Asn Ile Thr Cys Arg 370 375 380 Cys Ser Glu Asn Tyr Ser Gly Arg Phe Cys Glu Lys Lys Asn 385 390 395 <210> 34 <211> 468 <212> PRT <213> Cupiennius salei <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Cupiennius salei with a N-terminal secretion signal and a C-terminal anchoring signal <400> 34 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Phe Lys Ile Tyr Trp Asn Val Pro Thr Phe Gln Cys 20 25 30 Thr His Asn Tyr Lys Ile Asp Tyr Val Lys Leu Leu Ser Thr Tyr Gly 35 40 45 Ile Gln Val Asn Asp Gly Gly Lys Phe Gln Gly Asn Gln Val Thr Ile 50 55 60 Phe Tyr Glu Thr Gln Leu Gly Leu Tyr Pro Arg Ile Leu Lys Ser Gly 65 70 75 80 Lys Met Glu Asn Gly Gly Ile Pro Gln Arg Gly Asn Phe Glu Lys His 85 90 95 Leu Glu Lys Ala Ser Thr Asp Leu Gln Lys Val Ile Pro Trp Lys Glu 100 105 110 Phe Ser Gly Leu Gly Val Ile Asp Trp Glu Ala Trp Arg Pro Thr Trp 115 120 125 Glu Phe Asn Trp Glu Pro Leu Arg Ile Tyr Gln Thr Glu Ser Ile Lys 130 135 140 Arg Ala Lys Glu Leu His Pro Thr Ala Asn Asp Ser Ala Val Lys Glu 145 150 155 160 Ile Ala Glu Arg Gln Trp Glu Asp Ser Ala Lys Leu Tyr Met Leu Glu 165 170 175 Thr Leu Arg Leu Ala Lys Lys Leu Arg Pro Gln Ala Pro Trp Cys Tyr 180 185 190 Tyr Leu Phe Pro Asp Cys Tyr Asn Tyr Val Gly Lys Lys Pro Lys Asp 195 200 205 Phe Gln Cys Ser Ala Ser Ile Arg Lys Gly Asn Asp Lys Leu Ser Trp 210 215 220 Leu Trp Lys Asp Ser Thr Ala Leu Cys Pro Ser Ile Tyr Val Tyr Glu 225 230 235 240 Ser Gln Leu Asp Arg Tyr Ser Phe Glu Gln Arg Thr Trp Arg Asp Asn 245 250 255 Glu Lys Leu Arg Glu Ala Leu Arg Val Ala Thr Arg Thr Ser Lys Ile 260 265 270 Tyr Pro Tyr Val Asn Tyr Phe Asp Lys Glu Leu Ile Pro Glu Gln Glu 275 280 285 Val Trp Arg Met Leu Ala Gln Ala Ala Ala Val Gly Gly Ser Gly Ala 290 295 300 Val Ile Trp Gly Ser Ser Ala Ala Val Ala Ser Glu Glu Leu Cys Lys 305 310 315 320 Ser Leu Lys Gln Tyr Ile Ile Glu Thr Leu Gly Pro Ala Ala Glu Lys 325 330 335 Val Ala Trp Arg Ser Asp Leu Cys Ser Lys Glu Ile Cys Asn Asn Gln 340 345 350 Gly Arg Cys Thr Phe Pro Asp Asp Asp Tyr Ala Asn Ala Trp Lys Leu 355 360 365 Phe Thr Asp Asp Thr Val Lys Phe Tyr Ala Gly Asn Ile Thr Cys Arg 370 375 380 Cys Ser Glu Asn Tyr Ser Gly Arg Phe Cys Glu Lys Lys Asn Gly Ser 385 390 395 400 Ala Ile Ser Gln Ile Thr Asp Gly Gln Ile Gln Ala Thr Thr Thr Ala 405 410 415 Thr Thr Glu Ala Thr Thr Thr Ala Ala Pro Ser Ser Thr Val Glu Thr 420 425 430 Val Ser Pro Ser Ser Thr Glu Thr Ile Ser Gln Gln Thr Glu Asn Gly 435 440 445 Ala Ala Lys Ala Ala Val Gly Met Gly Ala Gly Ala Leu Ala Ala Ala 450 455 460 Ala Met Leu Leu 465 <210> 35 <211> 579 <212> PRT <213> Hirudo nipponia <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal <400> 35 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Met Lys Glu Ile Ala Val Thr Ile Asp Asp Lys Asn 20 25 30 Val Ile Ala Ser Val Ser Glu Ser Phe His Gly Val Ala Phe Asp Ala 35 40 45 Ser Leu Phe Ser Pro Lys Gly Leu Trp Ser Phe Val Asp Ile Thr Ser 50 55 60 Pro Lys Leu Phe Lys Leu Leu Glu Gly Leu Ser Pro Gly Tyr Phe Arg 65 70 75 80 Val Gly Gly Thr Phe Ala Asn Trp Leu Phe Phe Asp Leu Asp Glu Asn 85 90 95 Asn Lys Trp Lys Asp Tyr Trp Ala Phe Lys Asp Lys Thr Pro Glu Thr 100 105 110 Ala Thr Ile Thr Arg Arg Trp Leu Phe Arg Lys Gln Asn Asn Leu Lys 115 120 125 Lys Glu Thr Phe Asp Asp Leu Val Lys Leu Thr Lys Gly Ser Lys Met 130 135 140 Arg Leu Leu Phe Asp Leu Asn Ala Glu Val Arg Thr Gly Tyr Glu Ile 145 150 155 160 Gly Lys Lys Met Thr Ser Thr Trp Asp Ser Ser Glu Ala Glu Lys Leu 165 170 175 Phe Lys Tyr Cys Val Ser Lys Gly Tyr Gly Asp Asn Ile Asp Trp Glu 180 185 190 Leu Gly Asn Glu Pro Asp His Thr Ser Ala His Asn Leu Thr Glu Lys 195 200 205 Gln Val Gly Glu Asp Phe Lys Ala Leu His Lys Val Leu Glu Lys Tyr 210 215 220 Pro Thr Leu Asn Lys Gly Ser Leu Val Gly Pro Asp Val Gly Trp Met 225 230 235 240 Gly Val Ser Tyr Val Lys Gly Leu Ala Asp Gly Ala Gly Asp His Val 245 250 255 Thr Ala Phe Thr Leu His Gln Tyr Tyr Phe Asp Gly Asn Thr Ser Asp 260 265 270 Val Ser Thr Tyr Leu Asp Ala Thr Tyr Phe Lys Lys Leu Gln Gln Leu 275 280 285 Phe Asp Lys Val Lys Asp Val Leu Lys Asn Ser Pro His Lys Asp Lys 290 295 300 Pro Leu Trp Leu Gly Glu Thr Ser Ser Gly Tyr Asn Ser Gly Thr Lys 305 310 315 320 Asp Val Ser Asp Arg Tyr Val Ser Gly Phe Leu Thr Leu Asp Lys Leu 325 330 335 Gly Leu Ser Ala Ala Asn Asn Val Lys Val Val Ile Arg Gln Thr Ile 340 345 350 Tyr Asn Gly Tyr Tyr Gly Leu Leu Asp Lys Asn Thr Leu Glu Pro Asn 355 360 365 Pro Asp Tyr Trp Leu Met His Val His Asn Ser Leu Val Gly Asn Thr 370 375 380 Val Phe Lys Val Asp Val Ser Asp Pro Thr Asn Lys Ala Arg Val Tyr 385 390 395 400 Ala Gln Cys Thr Lys Thr Asn Ser Lys His Thr Gln Ser Arg Tyr Tyr 405 410 415 Lys Gly Ser Leu Thr Ile Phe Ala Leu Asn Val Gly Asp Glu Asp Val 420 425 430 Thr Leu Lys Ile Asp Gln Tyr Ser Gly Lys Lys Ile Tyr Ser Tyr Ile 435 440 445 Leu Thr Pro Glu Gly Gly Gln Leu Thr Ser Gln Lys Val Leu Leu Asn 450 455 460 Gly Lys Glu Leu Lys Leu Val Ser Asp Gln Leu Pro Glu Leu Asn Ala 465 470 475 480 Asp Glu Ser Lys Thr Ser Phe Thr Leu Ser Pro Lys Thr Phe Gly Phe 485 490 495 Phe Val Val Ser Asp Ala Asn Val Glu Ala Cys Lys Lys Gly Ser Ala 500 505 510 Ile Ser Gln Ile Thr Asp Gly Gln Ile Gln Ala Thr Thr Thr Ala Thr 515 520 525 Thr Glu Ala Thr Thr Thr Ala Ala Pro Ser Ser Thr Val Glu Thr Val 530 535 540 Ser Pro Ser Ser Thr Glu Thr Ile Ser Gln Gln Thr Glu Asn Gly Ala 545 550 555 560 Ala Lys Ala Ala Val Gly Met Gly Ala Gly Ala Leu Ala Ala Ala Ala 565 570 575 Met Leu Leu <210> 36 <211> 509 <212> PRT <213> Hirudo nipponia <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Hirudo Nipponia with a N-terminal secretion signal and a C-terminal anchoring signal <400> 36 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Met Lys Glu Ile Ala Val Thr Ile Asp Asp Lys Asn 20 25 30 Val Ile Ala Ser Val Ser Glu Ser Phe His Gly Val Ala Phe Asp Ala 35 40 45 Ser Leu Phe Ser Pro Lys Gly Leu Trp Ser Phe Val Asp Ile Thr Ser 50 55 60 Pro Lys Leu Phe Lys Leu Leu Glu Gly Leu Ser Pro Gly Tyr Phe Arg 65 70 75 80 Val Gly Gly Thr Phe Ala Asn Trp Leu Phe Phe Asp Leu Asp Glu Asn 85 90 95 Asn Lys Trp Lys Asp Tyr Trp Ala Phe Lys Asp Lys Thr Pro Glu Thr 100 105 110 Ala Thr Ile Thr Arg Arg Trp Leu Phe Arg Lys Gln Asn Asn Leu Lys 115 120 125 Lys Glu Thr Phe Asp Asp Leu Val Lys Leu Thr Lys Gly Ser Lys Met 130 135 140 Arg Leu Leu Phe Asp Leu Asn Ala Glu Val Arg Thr Gly Tyr Glu Ile 145 150 155 160 Gly Lys Lys Met Thr Ser Thr Trp Asp Ser Ser Glu Ala Glu Lys Leu 165 170 175 Phe Lys Tyr Cys Val Ser Lys Gly Tyr Gly Asp Asn Ile Asp Trp Glu 180 185 190 Leu Gly Asn Glu Pro Asp His Thr Ser Ala His Asn Leu Thr Glu Lys 195 200 205 Gln Val Gly Glu Asp Phe Lys Ala Leu His Lys Val Leu Glu Lys Tyr 210 215 220 Pro Thr Leu Asn Lys Gly Ser Leu Val Gly Pro Asp Val Gly Trp Met 225 230 235 240 Gly Val Ser Tyr Val Lys Gly Leu Ala Asp Gly Ala Gly Asp His Val 245 250 255 Thr Ala Phe Thr Leu His Gln Tyr Tyr Phe Asp Gly Asn Thr Ser Asp 260 265 270 Val Ser Thr Tyr Leu Asp Ala Thr Tyr Phe Lys Lys Leu Gln Gln Leu 275 280 285 Phe Asp Lys Val Lys Asp Val Leu Lys Asn Ser Pro His Lys Asp Lys 290 295 300 Pro Leu Trp Leu Gly Glu Thr Ser Ser Gly Tyr Asn Ser Gly Thr Lys 305 310 315 320 Asp Val Ser Asp Arg Tyr Val Ser Gly Phe Leu Thr Leu Asp Lys Leu 325 330 335 Gly Leu Ser Ala Ala Asn Asn Val Lys Val Val Ile Arg Gln Thr Ile 340 345 350 Tyr Asn Gly Tyr Tyr Gly Leu Leu Asp Lys Asn Thr Leu Glu Pro Asn 355 360 365 Pro Asp Tyr Trp Leu Met His Val His Asn Ser Leu Val Gly Asn Thr 370 375 380 Val Phe Lys Val Asp Val Ser Asp Pro Thr Asn Lys Ala Arg Val Tyr 385 390 395 400 Ala Gln Cys Thr Lys Thr Asn Ser Lys His Thr Gln Ser Arg Tyr Tyr 405 410 415 Lys Gly Ser Leu Thr Ile Phe Ala Leu Asn Val Gly Asp Glu Asp Val 420 425 430 Thr Leu Lys Ile Asp Gln Tyr Ser Gly Lys Lys Ile Tyr Ser Tyr Ile 435 440 445 Leu Thr Pro Glu Gly Gly Gln Leu Thr Ser Gln Lys Val Leu Leu Asn 450 455 460 Gly Lys Glu Leu Lys Leu Val Ser Asp Gln Leu Pro Glu Leu Asn Ala 465 470 475 480 Asp Glu Ser Lys Thr Ser Phe Thr Leu Ser Pro Lys Thr Phe Gly Phe 485 490 495 Phe Val Val Ser Asp Ala Asn Val Glu Ala Cys Lys Lys 500 505 <210> 37 <211> 401 <212> PRT <213> Loxosceles intermedia <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal <400> 37 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Phe Asp Val Phe Trp Asn Val Pro Ser Gln Gln Cys 20 25 30 Lys Lys Tyr Gly Met Lys Phe Val Pro Leu Leu Glu Gln Tyr Ser Ile 35 40 45 Leu Val Asn Lys Glu Asp Asn Phe Lys Gly Asp Lys Ile Thr Ile Phe 50 55 60 Tyr Glu Ser Gln Leu Gly Leu Tyr Pro His Ile Gly Ala Asn Asp Glu 65 70 75 80 Ser Phe Asn Gly Gly Ile Pro Gln Leu Gly Asp Leu Lys Ala His Leu 85 90 95 Glu Lys Ser Ala Val Asp Ile Arg Arg Asp Ile Leu Asp Lys Ser Ala 100 105 110 Thr Gly Leu Arg Ile Ile Asp Trp Glu Ala Trp Arg Pro Ile Trp Glu 115 120 125 Phe Asn Trp Ser Ser Leu Arg Lys Tyr Gln Asp Lys Met Lys Lys Val 130 135 140 Val Arg Gln Phe Asn Pro Thr Ala His Glu Ser Thr Val Ala Lys Leu 145 150 155 160 Ala His Asn Glu Trp Glu Asn Ser Ser Lys Ser Trp Met Leu Ser Thr 165 170 175 Leu Gln Leu Gly Lys Gln Leu Arg Pro Asn Ser Val Trp Cys Tyr Tyr 180 185 190 Leu Phe Pro Asp Cys Tyr Asn Tyr Asp Gly Asn Ser Val Gln Glu Phe 195 200 205 Gln Cys Ser Glu Ala Ile Arg Lys Gly Asn Asp Arg Leu Lys Trp Leu 210 215 220 Trp Glu Glu Ser Thr Ala Val Cys Pro Ser Ile Tyr Ile Lys Glu Gly 225 230 235 240 Gln Leu Thr Asn Tyr Thr Leu Gln Lys Arg Ile Trp Phe Thr Asn Gly 245 250 255 Arg Leu Gln Glu Ala Leu Arg Val Ala Gln Pro Lys Ala Arg Ile Tyr 260 265 270 Pro Tyr Ile Asn Tyr Ser Ile Lys Pro Gly Met Met Val Pro Glu Val 275 280 285 Glu Phe Trp Arg Leu Ile Ala Gln Ile Ala Ser Leu Gly Met Asp Gly 290 295 300 Ala Val Ile Trp Gly Ser Ser Ala Ser Val Gly Ser Lys Asn His Cys 305 310 315 320 Ala Gln Leu Met Lys Tyr Ile Ala Asp Val Leu Gly Pro Ala Thr Leu 325 330 335 Arg Ile Lys Glu Asn Val Ala Arg Cys Ser Lys Gln Ala Cys Ser Gly 340 345 350 Arg Gly Arg Cys Thr Trp Pro Lys Asp Thr Ser Val Ile Ala Trp Lys 355 360 365 Phe Leu Val Glu Lys Glu Asp Tyr Asp Phe Tyr Leu Gly Asp Ile Glu 370 375 380 Cys Lys Cys Val Glu Gly Tyr Glu Gly Arg Tyr Cys Glu Gln Lys Thr 385 390 395 400 Lys <210> 38 <211> 471 <212> PRT <213> Loxosceles intermedia <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Loxosceles intermedia with a N-terminal secretion signal and a C-terminal anchoring signal <400> 38 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Phe Asp Val Phe Trp Asn Val Pro Ser Gln Gln Cys 20 25 30 Lys Lys Tyr Gly Met Lys Phe Val Pro Leu Leu Glu Gln Tyr Ser Ile 35 40 45 Leu Val Asn Lys Glu Asp Asn Phe Lys Gly Asp Lys Ile Thr Ile Phe 50 55 60 Tyr Glu Ser Gln Leu Gly Leu Tyr Pro His Ile Gly Ala Asn Asp Glu 65 70 75 80 Ser Phe Asn Gly Gly Ile Pro Gln Leu Gly Asp Leu Lys Ala His Leu 85 90 95 Glu Lys Ser Ala Val Asp Ile Arg Arg Asp Ile Leu Asp Lys Ser Ala 100 105 110 Thr Gly Leu Arg Ile Ile Asp Trp Glu Ala Trp Arg Pro Ile Trp Glu 115 120 125 Phe Asn Trp Ser Ser Leu Arg Lys Tyr Gln Asp Lys Met Lys Lys Val 130 135 140 Val Arg Gln Phe Asn Pro Thr Ala His Glu Ser Thr Val Ala Lys Leu 145 150 155 160 Ala His Asn Glu Trp Glu Asn Ser Ser Lys Ser Trp Met Leu Ser Thr 165 170 175 Leu Gln Leu Gly Lys Gln Leu Arg Pro Asn Ser Val Trp Cys Tyr Tyr 180 185 190 Leu Phe Pro Asp Cys Tyr Asn Tyr Asp Gly Asn Ser Val Gln Glu Phe 195 200 205 Gln Cys Ser Glu Ala Ile Arg Lys Gly Asn Asp Arg Leu Lys Trp Leu 210 215 220 Trp Glu Glu Ser Thr Ala Val Cys Pro Ser Ile Tyr Ile Lys Glu Gly 225 230 235 240 Gln Leu Thr Asn Tyr Thr Leu Gln Lys Arg Ile Trp Phe Thr Asn Gly 245 250 255 Arg Leu Gln Glu Ala Leu Arg Val Ala Gln Pro Lys Ala Arg Ile Tyr 260 265 270 Pro Tyr Ile Asn Tyr Ser Ile Lys Pro Gly Met Met Val Pro Glu Val 275 280 285 Glu Phe Trp Arg Leu Ile Ala Gln Ile Ala Ser Leu Gly Met Asp Gly 290 295 300 Ala Val Ile Trp Gly Ser Ser Ala Ser Val Gly Ser Lys Asn His Cys 305 310 315 320 Ala Gln Leu Met Lys Tyr Ile Ala Asp Val Leu Gly Pro Ala Thr Leu 325 330 335 Arg Ile Lys Glu Asn Val Ala Arg Cys Ser Lys Gln Ala Cys Ser Gly 340 345 350 Arg Gly Arg Cys Thr Trp Pro Lys Asp Thr Ser Val Ile Ala Trp Lys 355 360 365 Phe Leu Val Glu Lys Glu Asp Tyr Asp Phe Tyr Leu Gly Asp Ile Glu 370 375 380 Cys Lys Cys Val Glu Gly Tyr Glu Gly Arg Tyr Cys Glu Gln Lys Thr 385 390 395 400 Lys Gly Ser Ala Ile Ser Gln Ile Thr Asp Gly Gln Ile Gln Ala Thr 405 410 415 Thr Thr Ala Thr Thr Glu Ala Thr Thr Thr Ala Ala Pro Ser Ser Thr 420 425 430 Val Glu Thr Val Ser Pro Ser Ser Thr Glu Thr Ile Ser Gln Gln Thr 435 440 445 Glu Asn Gly Ala Ala Lys Ala Ala Val Gly Met Gly Ala Gly Ala Leu 450 455 460 Ala Ala Ala Ala Met Leu Leu 465 470 <210> 39 <211> 405 <212> PRT <213> Tityus serrulatus <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal <400> 39 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Ala Asp Phe Lys Val Tyr Trp Glu Val Pro Ser Phe 20 25 30 Leu Cys Ser Lys Arg Phe Lys Ile Asn Val Thr Glu Val Leu Thr Ser 35 40 45 His Glu Ile Leu Val Asn Gln Gly Glu Ser Phe Asn Gly Asp Lys Ile 50 55 60 Val Ile Phe Tyr Glu Asn Gln Leu Gly Lys Tyr Pro His Ile Asp Ser 65 70 75 80 Asn Asn Val Glu Ile Asn Gly Gly Ile Leu Gln Val Ala Asp Leu Ala 85 90 95 Lys His Leu Lys Val Ala Lys Asp Asn Ile Thr Lys Phe Val Pro Asn 100 105 110 Pro Asn Phe Asn Gly Val Gly Val Ile Asp Trp Glu Ala Trp Arg Pro 115 120 125 Ser Trp Glu Phe Asn Trp Gly Lys Leu Lys Val Tyr Lys Glu Lys Ser 130 135 140 Ile Asp Leu Val Lys Ser Lys His Pro Glu Trp Pro Ser Asp Arg Val 145 150 155 160 Glu Lys Val Ala Lys Glu Glu Trp Glu Glu Ser Ala Lys Glu Trp Met 165 170 175 Val Lys Thr Leu Lys Leu Ala Gln Glu Met Arg Pro Asn Ala Val Trp 180 185 190 Cys Tyr Tyr Leu Phe Pro Asp Cys Tyr Asn Tyr Phe Gly Lys Asp Gln 195 200 205 Pro Ser Gln Phe Ser Cys Ser Ser Arg Ile Gln Lys Glu Asn Ser Arg 210 215 220 Leu Ser Trp Leu Trp Asn Gln Ser Thr Ala Ile Cys Leu Ser Ile Tyr 225 230 235 240 Ile Gln Glu Ser His Val Thr Lys Tyr Asn Met Ser Gln Arg Thr Trp 245 250 255 Trp Ile Asp Ala Arg Leu Arg Glu Ala Ile Arg Val Ser Glu His Arg 260 265 270 Pro Asn Ile Pro Ile Tyr Pro Tyr Ile Asn Tyr Ile Leu Pro Gly Thr 275 280 285 Asn Gln Thr Val Pro Ala Met Asp Phe Lys Arg Thr Leu Gly Gln Ile 290 295 300 Ala Ser Leu Gly Leu Asp Gly Ala Leu Leu Trp Gly Ser Ser Tyr His 305 310 315 320 Val Leu Thr Glu Ser Gln Cys Lys Ile Thr Ser Asp Tyr Val Lys Ser 325 330 335 Val Ile Ala Pro Thr Val Ala Thr Val Val Leu Asn Thr Asn Arg Cys 340 345 350 Ser Gln Ile Ile Cys Lys Gly Arg Gly Asn Cys Val Trp Pro Glu Glu 355 360 365 Pro Phe Ser Ser Trp Lys Tyr Leu Val Asp Pro Lys Met Pro Val Phe 370 375 380 Lys Pro Thr Asn Ile His Cys Lys Cys Lys Gly Tyr Leu Gly Arg Tyr 385 390 395 400 Cys Glu Ile Pro Lys 405 <210> 40 <211> 475 <212> PRT <213> Tityus serrulatus <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Tityus serrulatus with a N-terminal secretion signal and a C-terminal anchoring signal <400> 40 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Ala Asp Phe Lys Val Tyr Trp Glu Val Pro Ser Phe 20 25 30 Leu Cys Ser Lys Arg Phe Lys Ile Asn Val Thr Glu Val Leu Thr Ser 35 40 45 His Glu Ile Leu Val Asn Gln Gly Glu Ser Phe Asn Gly Asp Lys Ile 50 55 60 Val Ile Phe Tyr Glu Asn Gln Leu Gly Lys Tyr Pro His Ile Asp Ser 65 70 75 80 Asn Asn Val Glu Ile Asn Gly Gly Ile Leu Gln Val Ala Asp Leu Ala 85 90 95 Lys His Leu Lys Val Ala Lys Asp Asn Ile Thr Lys Phe Val Pro Asn 100 105 110 Pro Asn Phe Asn Gly Val Gly Val Ile Asp Trp Glu Ala Trp Arg Pro 115 120 125 Ser Trp Glu Phe Asn Trp Gly Lys Leu Lys Val Tyr Lys Glu Lys Ser 130 135 140 Ile Asp Leu Val Lys Ser Lys His Pro Glu Trp Pro Ser Asp Arg Val 145 150 155 160 Glu Lys Val Ala Lys Glu Glu Trp Glu Glu Ser Ala Lys Glu Trp Met 165 170 175 Val Lys Thr Leu Lys Leu Ala Gln Glu Met Arg Pro Asn Ala Val Trp 180 185 190 Cys Tyr Tyr Leu Phe Pro Asp Cys Tyr Asn Tyr Phe Gly Lys Asp Gln 195 200 205 Pro Ser Gln Phe Ser Cys Ser Ser Arg Ile Gln Lys Glu Asn Ser Arg 210 215 220 Leu Ser Trp Leu Trp Asn Gln Ser Thr Ala Ile Cys Leu Ser Ile Tyr 225 230 235 240 Ile Gln Glu Ser His Val Thr Lys Tyr Asn Met Ser Gln Arg Thr Trp 245 250 255 Trp Ile Asp Ala Arg Leu Arg Glu Ala Ile Arg Val Ser Glu His Arg 260 265 270 Pro Asn Ile Pro Ile Tyr Pro Tyr Ile Asn Tyr Ile Leu Pro Gly Thr 275 280 285 Asn Gln Thr Val Pro Ala Met Asp Phe Lys Arg Thr Leu Gly Gln Ile 290 295 300 Ala Ser Leu Gly Leu Asp Gly Ala Leu Leu Trp Gly Ser Ser Tyr His 305 310 315 320 Val Leu Thr Glu Ser Gln Cys Lys Ile Thr Ser Asp Tyr Val Lys Ser 325 330 335 Val Ile Ala Pro Thr Val Ala Thr Val Val Leu Asn Thr Asn Arg Cys 340 345 350 Ser Gln Ile Ile Cys Lys Gly Arg Gly Asn Cys Val Trp Pro Glu Glu 355 360 365 Pro Phe Ser Ser Trp Lys Tyr Leu Val Asp Pro Lys Met Pro Val Phe 370 375 380 Lys Pro Thr Asn Ile His Cys Lys Cys Lys Gly Tyr Leu Gly Arg Tyr 385 390 395 400 Cys Glu Ile Pro Lys Gly Ser Ala Ile Ser Gln Ile Thr Asp Gly Gln 405 410 415 Ile Gln Ala Thr Thr Thr Ala Thr Thr Glu Ala Thr Thr Thr Ala Ala 420 425 430 Pro Ser Ser Thr Val Glu Thr Val Ser Pro Ser Ser Thr Glu Thr Ile 435 440 445 Ser Gln Gln Thr Glu Asn Gly Ala Ala Lys Ala Ala Val Gly Met Gly 450 455 460 Ala Gly Ala Leu Ala Ala Ala Ala Met Leu Leu 465 470 475 <210> 41 <211> 360 <212> PRT <213> Vespa magnifica <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal <400> 41 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Asp Ser Cys Gly Ser Asn Cys Glu Lys Ser Glu Arg 20 25 30 Pro Lys Arg Val Phe Asn Ile Tyr Trp Asn Val Pro Thr Phe Met Cys 35 40 45 His Gln Tyr Gly Leu Tyr Phe Asp Glu Val Thr Asn Phe Asn Ile Lys 50 55 60 His Asn Ser Lys Asp Asn Phe Gln Gly Asp Lys Ile Ala Ile Phe Tyr 65 70 75 80 Asp Pro Gly Glu Phe Pro Ala Leu Leu Pro Leu Asn Tyr Gly Lys Tyr 85 90 95 Lys Ile Arg Asn Gly Gly Val Pro Gln Glu Gly Asn Ile Thr Ile His 100 105 110 Leu Gln Arg Phe Ile Glu Tyr Leu Asp Lys Thr Tyr Pro Asn Arg Asn 115 120 125 Phe Ser Gly Ile Gly Val Ile Asp Phe Glu Arg Trp Arg Pro Ile Phe 130 135 140 Arg Gln Asn Trp Gly Asn Met Lys Ile Tyr Lys Asn Phe Ser Ile Asp 145 150 155 160 Leu Val Arg Lys Glu His Pro Phe Trp Asn Lys Lys Met Ile Glu Leu 165 170 175 Glu Ala Ser Lys Arg Phe Glu Lys Tyr Ala Arg Leu Phe Met Glu Glu 180 185 190 Thr Leu Lys Leu Ala Lys Lys Thr Arg Lys Gln Ala Asp Trp Gly Tyr 195 200 205 Tyr Gly Tyr Pro Tyr Cys Phe Asn Met Ser Pro Thr Asn Phe Val Pro 210 215 220 Asp Cys Asp Val Thr Ala Arg Asp Glu Asn Asn Glu Met Ser Trp Leu 225 230 235 240 Phe Asn Asn Gln Asn Val Leu Leu Pro Ser Val Tyr Ile Arg Arg Glu 245 250 255 Leu Thr Pro Asp Gln Arg Ile Gly Leu Val Gln Gly Arg Val Lys Glu 260 265 270 Ala Val Arg Ile Ser Asn Lys Leu Lys His Ser Pro Lys Val Phe Ser 275 280 285 Tyr Trp Trp Tyr Val Tyr Gln Asp Glu Thr Asn Thr Phe Leu Thr Glu 290 295 300 Thr Asp Val Lys Lys Thr Phe Gln Glu Ile Val Ile Asn Gly Gly Asp 305 310 315 320 Gly Ile Ile Ile Trp Gly Ser Ser Ser Asp Val Asn Ser Leu Ser Lys 325 330 335 Cys Thr Arg Leu Arg Glu Tyr Leu Leu Thr Val Leu Gly Pro Ile Ala 340 345 350 Val Asn Val Thr Glu Ala Val Asn 355 360 <210> 42 <211> 428 <212> PRT <213> Vespa magnifica <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal and a C-terminal anchoring signal <220> <223> Amino acid sequence of hyaluronidase (HYAL) originating from Vespa magnifica with a N-terminal secretion signal and a C-terminal anchoring signal <400> 42 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala Asp Ser Cys Gly Ser Asn Cys Glu Lys Ser Glu Arg 20 25 30 Pro Lys Arg Val Phe Asn Ile Tyr Trp Asn Val Pro Thr Phe Met Cys 35 40 45 His Gln Tyr Gly Leu Tyr Phe Asp Glu Val Thr Asn Phe Asn Ile Lys 50 55 60 His Asn Ser Lys Asp Asn Phe Gln Gly Asp Lys Ile Ala Ile Phe Tyr 65 70 75 80 Asp Pro Gly Glu Phe Pro Ala Leu Leu Pro Leu Asn Tyr Gly Lys Tyr 85 90 95 Lys Ile Arg Asn Gly Gly Val Pro Gln Glu Gly Asn Ile Thr Ile His 100 105 110 Leu Gln Arg Phe Ile Glu Tyr Leu Asp Lys Thr Tyr Pro Asn Arg Asn 115 120 125 Phe Ser Gly Ile Gly Val Ile Asp Phe Glu Arg Trp Arg Pro Ile Phe 130 135 140 Arg Gln Asn Trp Gly Asn Met Lys Ile Tyr Lys Asn Phe Ser Ile Asp 145 150 155 160 Leu Val Arg Lys Glu His Pro Phe Trp Asn Lys Lys Met Ile Glu Leu 165 170 175 Glu Ala Ser Lys Arg Phe Glu Lys Tyr Ala Arg Leu Phe Met Glu Glu 180 185 190 Thr Leu Lys Leu Ala Lys Lys Thr Arg Lys Gln Ala Asp Trp Gly Tyr 195 200 205 Tyr Gly Tyr Pro Tyr Cys Phe Asn Met Ser Pro Thr Asn Phe Val Pro 210 215 220 Asp Cys Asp Val Thr Ala Arg Asp Glu Asn Asn Glu Met Ser Trp Leu 225 230 235 240 Phe Asn Asn Gln Asn Val Leu Leu Pro Ser Val Tyr Ile Arg Arg Glu 245 250 255 Leu Thr Pro Asp Gln Arg Ile Gly Leu Val Gln Gly Arg Val Lys Glu 260 265 270 Ala Val Arg Ile Ser Asn Lys Leu Lys His Ser Pro Lys Val Phe Ser 275 280 285 Tyr Trp Trp Tyr Val Tyr Gln Asp Glu Thr Asn Thr Phe Leu Thr Glu 290 295 300 Thr Asp Val Lys Lys Thr Phe Gln Glu Ile Val Ile Asn Gly Gly Asp 305 310 315 320 Gly Ile Ile Ile Trp Gly Ser Ser Ser Asp Val Asn Ser Leu Ser Lys 325 330 335 Cys Thr Arg Leu Arg Glu Tyr Leu Leu Thr Val Leu Gly Pro Ile Ala 340 345 350 Val Asn Val Thr Glu Ala Val Asn Ala Ile Ser Gln Ile Thr Asp Gly 355 360 365 Gln Ile Gln Ala Thr Thr Thr Ala Thr Thr Glu Ala Thr Thr Thr Ala 370 375 380 Ala Pro Ser Ser Thr Val Glu Thr Val Ser Pro Ser Ser Thr Glu Thr 385 390 395 400 Ile Ser Gln Gln Thr Glu Asn Gly Ala Ala Lys Ala Ala Val Gly Met 405 410 415 Gly Ala Gly Ala Leu Ala Ala Ala Ala Met Leu Leu 420 425 <210> 43 <211> 60 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of the secretion sequence added in 5’ <220> <223> Nucleic acid sequence of the secretion sequence added in 5’ <400> 43 atgcaattta gcacagtcgc atcagtagcc ttcgttgcct tggccaactt cgtggcagca 60 <210> 44 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of the secretion sequence added in N-ter <220> <223> Amino acid sequence of the secretion sequence added in N-ter <400> 44 Met Gln Phe Ser Thr Val Ala Ser Val Ala Phe Val Ala Leu Ala Asn 1 5 10 15 Phe Val Ala Ala 20 <210> 45 <211> 213 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of the anchoring sequence added in 3’ <220> <223> Nucleic acid sequence of the anchoring sequence added in 3’ <400> 45 ggatccgcca tttctcaaat cactgacggt caaatccaag ctactaccac tgctaccacc 60 gaagctacca ccactgctgc cccatcttcc accgttgaaa ctgtttctcc atccagcacc 120 gaaactatct ctcaacaaac tgaaaatggt gctgctaagg ccgctgtcgg tatgggtgcc 180 ggtgctctag ctgctgctgc tatgttgtta taa 213 <210> 46 <211> 68 <212> PRT <213> Artificial Sequence <220> <223> Amino acid sequence of the anchoring sequence added in C-ter <220> <223> Amino acid sequence of the anchoring sequence added in C-ter <400> 46 Ala Ile Ser Gln Ile Thr Asp Gly Gln Ile Gln Ala Thr Thr Thr Ala 1 5 10 15 Thr Thr Glu Ala Thr Thr Thr Ala Ala Pro Ser Ser Thr Val Glu Thr 20 25 30 Val Ser Pro Ser Ser Thr Glu Thr Ile Ser Gln Gln Thr Glu Asn Gly 35 40 45 Ala Ala Lys Ala Ala Val Gly Met Gly Ala Gly Ala Leu Ala Ala Ala 50 55 60 Ala Met Leu Leu 65 <210> 47 <211> 2154 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from <220> <223> Nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from <400> 47 atgtgtggta tctttggtta ctgcaattat ctagtggaaa gatccagagg agaaattatc 60 gacaccttag tggatggttt acaaagatta gaatatagag gctatgattc caccggtatt 120 gctatcgatg gtgacgaagc tgattctact ttcatctata agcaaatcgg taaagtgagt 180 gctttgaaag aggagattac taagcaaaat ccgaacagag acgttacttt tgtctctcat 240 tgtggtattg cgcatactag atgggctact cacggtcgac cagaacaagt taactgtcac 300 cctcaaagat ctgacccaga agaccaattt gtggtcgttc ataatggtat catcacaaat 360 tttagagaac tgaagactct tttaattaac aaaggttata aattcgaaag tgataccgat 420 accgagtgta ttgctaaact atatttgcat ttatacaata caaatttaca aaatgggcat 480 gacttagatt tccacgaatt aaccaagcta gttcttttag aactagaagg ttcatacggg 540 ttattatgta aatcttgtca ctatcctaat gaggttatcg ccactagaaa agggtcccct 600 ttactgattg gtgtcaaatc tgaaaaaaaa ctaaaagtcg acttcgtgga tgtggaattt 660 cccgaagaaa acgctggtca accggaaatt ccattgaaat ctaacaacaa atcatttggc 720 ttgggcccaa agaaagctcg tgaatttgaa gctggttccc aaaatgccaa tttactacca 780 attgccgcca atgaatttaa cttgagacat tctcaatcca gggctttcct atcagaagat 840 ggatctccaa caccggtgga attttttgtt tcttcggatg cggcatctgt tgttaaacat 900 accaagaagg tgctattttt agaagatgac gatttggctc atatttacga tggtgagtta 960 catattcata gatctagaag agaagtaggc gcatcaatga caaggtccat tcaaacttta 1020 gagatggagt tagctcagat catgaagggc ccttacgacc attttatgca aaaggaaatc 1080 tatgagcaac cagaatctac tttcaatact atgagaggta gaatcgacta tgaaaataat 1140 aaagtgatat tgggtggttt aaaggcatgg ttaccagttg tcagaagagc acggagactg 1200 atcatgatcg catgcggtac ttcttatcat tcatgtttgg ctactcgtgc tatcttcgaa 1260 gaattatcag atatcccagt tagtgtggaa ttagcgtctg actttctgga cagaaaatgc 1320 cctgtcttca gagacgatgt atgcgtgttt gtttcacaaa gtggtgaaac tgcggatacc 1380 atgctggctc taaattattg tttagaaaga ggagccttaa ctgtcggaat tgttaacagt 1440 gttggttctt ctatctctcg tgtcacccac tgtggtgttc atattaacgc tggtcctgaa 1500 attggtgttg cctctacaaa agcttatact tcccagtata ttgccttagt gatgtttgct 1560 ctatcgctgt cagatgaccg tgtatcgaaa atagacagaa gaattgaaat cattcaaggc 1620 ttgaagttaa tcccgggcca aattaagcag gtattaaagc tggaaccaag aataaaaaag 1680 ctctgtgcga ctgaattaaa ggatcaaaaa tctctattgt tattgggtag aggttaccaa 1740 tttgctgctg ctctggaagg tgctttgaag atcaaagaaa tttcttatat gcattctgaa 1800 ggtgttttgg caggtgagtt gaagcacggt gtcttggcct tggtggacga aaacttgcca 1860 atcattgctt ttggtaccag agactctcta ttccctaaag tagtttcctc tattgagcaa 1920 gttactgtaa gaaagggcca tccaattatt atttgtaacg aaaatgatga agtgtgggcg 1980 caaaaatcta aatcaatcga cctgcaaacc ttagaagttc cacaaactgt tgattgttta 2040 caaggtctaa ttaatattat tccattacaa ctaatgtcat attggttggc tgttaataaa 2100 gggattgatg ttgattttcc aagaaacttg gctaaatctg ttaccgtcga ataa 2154 <210> 48 <211> 1788 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <220> <223> Reencoded nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <400> 48 atgtgcggga tcttcggtgc tgtgtcgaac aataatagca tagaagtttc catcaagggt 60 atacagaagc tagagtaccg cgggtacgat tcgtgtggaa tagcctatac agacggaggc 120 gccattgaac ggatcaggtc aatagatggg atcgacgact taaggaagaa aacaataaca 180 gagtcttccc cggtagctat cgctcattca aggtggagta ccactgggat tccaagtgtt 240 gtgaacgccc acccgcacat ctctcggggc acgtctggat gcgagtcgcg cattgcagtc 300 gtacacaatg gcattattga aaattaccag cagatccgca agtacttgat aaatcttggg 360 tatactttcg attcacagac cgacacggaa gtcattgctc atctaataga ctcgcagtat 420 aacgggaaca tcctgcacac cgtccaaatg gcagttaagc acctcaaagg aagctacgcc 480 atagcagtca tgtgccataa ggagtccggg aagatcgtgg tggcaaaaca gaagagtccg 540 ttggtattgg gtataggaag cgacggagct tattatatcg catcggacgt attggctctt 600 cccactaaca aagtggtcta cataagtgac ggattctctg ctgagttaag tcctggatcg 660 atgactatct atgatctgga tggcaataag gtagaatacg aggtggaaga cgttgaaatg 720 gagcagacgt cgatgtcttt agataacttt gatcactaca tgatcaaaga gatcaatgaa 780 cagccgatat caattctcaa tactattaag aacaaaggat tctatgcaga gatatttggt 840 gatctagcac atgaaatatt tcagaagatc gacaatatat tgattctagc ctgcggaact 900 tcgtatcatg caggtctcgt cgggaagcaa tggatagaga ccatctcacg catcccggtg 960 gatgttcata ttgcaagcga gtatgagccg acaattccac gagccaatac acttgtgata 1020 acgattagtc aaagcggcga gactgcggat acaatcgcag cccttcaacg agcgcaaaac 1080 gcaggaatga tatacacttt gtgtatctgc aacagcccca agtcaactct agtgcgcgag 1140 tcgataatga aatacataac caaatgcggt tcagaagttt ctgttgccag tactaaagca 1200 tttacgtcgc aacttgtggt gttgtatatg cttgcgaacg ttcttgcgaa caaaactgat 1260 gatctgctag gggatcttcc tcaagctatc gagagagtaa tctgtcttac taatgacgaa 1320 atgaagcgtt gggcggatga aatttgtact gccaaaagtg ccatcttctt agggagagga 1380 ctgaacgcac ctgtagcatt tgagggcgcg ctaaagttga aggaaatctc atatatccat 1440 gccgagggtt tcctcggtgg agagttgaag catgggccgc tggctttgtt ggacgataag 1500 atccctgtaa tcgtgactgt tgctgaccac gcttatttgg atcacattaa ggctaacatc 1560 gacgaggtat tggcacgaaa cgttaccgta tacgcgatcg tcgatcagta tgttaatatt 1620 gaaccacagg agcgactgca tgtagtaaaa gtgccgtttg tgagtaaaga gttttctccc 1680 atcatccaca caattccgat gcaattgtta tcgtattatg tcgcgatcaa gctgggcaag 1740 aacgttgaca agccacgtaa cctggcgaaa agtgttacaa cattctaa 1788 <210> 49 <211> 1788 <212> DNA <213> Paramecium bursaria Chlorella virus 1 <220> <223> Reencoded nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <220> <223> Reencoded nucleic acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <400> 49 atgtgtggca tctttggagc agtgtcaaac aacaactcta tcgaggtgtc aatcaagggt 60 attcagaagc tagaatatcg tgggtatgat tcgtgcggta ttgcgtatac agatgggggt 120 gcgattgagc gtatacgttc tattgacggc attgacgatc tgcgtaagaa aacaatcaca 180 gaatcatcac cagtggccat tgctcactcg cggtggagca ccactggaat tccatcagtg 240 gtgaacgcac atcctcatat ttctcgcgga accagtgggt gtgagtctcg tatcgcggta 300 gtccacaacg gtatcattga aaactatcag cagatccgaa aatatctcat caatcttggt 360 tatacgtttg atagtcaaac ggacacagag gtcattgcgc atttgattga ttctcagtac 420 aatgggaata tcttgcacac cgtccaaatg gctgtcaagc acctgaaggg ctcttatgcc 480 attgcagtta tgtgtcataa agagtctggt aaaatagtcg tggcgaaaca gaagtcaccc 540 ctcgtacttg gaatcggctc agatggtgct tactacatcg cttcggacgt gctggcgctg 600 ccgacaaata aagttgttta tatttcagac ggtttctccg cagaactatc tccagggagt 660 atgaccattt acgatcttga tggaaataaa gtagaatatg aagtagagga cgttgaaatg 720 gaacaaacta gtatgtctct cgataacttt gatcattaca tgattaagga aattaatgag 780 caaccaatca gtattctaaa cactataaaa aataaagggt tctatgcaga aatattcggt 840 gatttggctc atgaaatctt ccaaaaaata gacaacatcc tgatactggc ttgtggtaca 900 agttatcacg ccggtcttgt aggaaaacag tggatagaga ccatctctag aatccccgtg 960 gatgttcaca tcgcgagtga atacgaacct actattccga gagcgaacac attggtaatc 1020 actatttcac agtcgggtga aactgcggac acgatagcgg ctttgcaacg ggcccaaaac 1080 gccgggatga tttatacatt gtgtatttgc aattcaccaa agagcactct tgttcgtgag 1140 agcattatga agtacatcac gaaatgtggt tctgaggtgt cagtggcatc aacgaaggcg 1200 tttacttctc agctcgtagt actgtacatg ctggcaaacg tattggcaaa taaaaccgat 1260 gatttgctgg gagacctccc acaggcaata gaacgggtaa tttgtttgac aaatgacgaa 1320 atgaaacgat gggccgacga aatttgcact gcgaaatccg cgatcttcct gggaagagga 1380 ctaaacgcac cagttgcctt tgagggagcg ttgaagctca aagaaatctc ttacattcat 1440 gcagagggct tcctgggagg tgagttgaaa cacggccccc tcgcactcct tgatgacaag 1500 attcctgtta tcgtaaccgt agcagatcat gcttatttgg accatatcaa agcaaatatc 1560 gacgaagtgc ttgcgaggaa cgttacggta tacgccatag tagaccagta tgtgaacatc 1620 gagccccagg aacgccttca cgtcgtcaag gttccgtttg tatccaaaga attttctccg 1680 ataattcaca ctatcccgat gcaactgctt tcgtattacg tggcaattaa gcttggaaag 1740 aacgttgaca aaccaaggaa tcttgcaaaa tccgtgacta ccttttaa 1788 <210> 50 <211> 717 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Saccharomyces cerevisiae <400> 50 Met Cys Gly Ile Phe Gly Tyr Cys Asn Tyr Leu Val Glu Arg Ser Arg 1 5 10 15 Gly Glu Ile Ile Asp Thr Leu Val Asp Gly Leu Gln Arg Leu Glu Tyr 20 25 30 Arg Gly Tyr Asp Ser Thr Gly Ile Ala Ile Asp Gly Asp Glu Ala Asp 35 40 45 Ser Thr Phe Ile Tyr Lys Gln Ile Gly Lys Val Ser Ala Leu Lys Glu 50 55 60 Glu Ile Thr Lys Gln Asn Pro Asn Arg Asp Val Thr Phe Val Ser His 65 70 75 80 Cys Gly Ile Ala His Thr Arg Trp Ala Thr His Gly Arg Pro Glu Gln 85 90 95 Val Asn Cys His Pro Gln Arg Ser Asp Pro Glu Asp Gln Phe Val Val 100 105 110 Val His Asn Gly Ile Ile Thr Asn Phe Arg Glu Leu Lys Thr Leu Leu 115 120 125 Ile Asn Lys Gly Tyr Lys Phe Glu Ser Asp Thr Asp Thr Glu Cys Ile 130 135 140 Ala Lys Leu Tyr Leu His Leu Tyr Asn Thr Asn Leu Gln Asn Gly His 145 150 155 160 Asp Leu Asp Phe His Glu Leu Thr Lys Leu Val Leu Leu Glu Leu Glu 165 170 175 Gly Ser Tyr Gly Leu Leu Cys Lys Ser Cys His Tyr Pro Asn Glu Val 180 185 190 Ile Ala Thr Arg Lys Gly Ser Pro Leu Leu Ile Gly Val Lys Ser Glu 195 200 205 Lys Lys Leu Lys Val Asp Phe Val Asp Val Glu Phe Pro Glu Glu Asn 210 215 220 Ala Gly Gln Pro Glu Ile Pro Leu Lys Ser Asn Asn Lys Ser Phe Gly 225 230 235 240 Leu Gly Pro Lys Lys Ala Arg Glu Phe Glu Ala Gly Ser Gln Asn Ala 245 250 255 Asn Leu Leu Pro Ile Ala Ala Asn Glu Phe Asn Leu Arg His Ser Gln 260 265 270 Ser Arg Ala Phe Leu Ser Glu Asp Gly Ser Pro Thr Pro Val Glu Phe 275 280 285 Phe Val Ser Ser Asp Ala Ala Ser Val Val Lys His Thr Lys Lys Val 290 295 300 Leu Phe Leu Glu Asp Asp Asp Leu Ala His Ile Tyr Asp Gly Glu Leu 305 310 315 320 His Ile His Arg Ser Arg Arg Glu Val Gly Ala Ser Met Thr Arg Ser 325 330 335 Ile Gln Thr Leu Glu Met Glu Leu Ala Gln Ile Met Lys Gly Pro Tyr 340 345 350 Asp His Phe Met Gln Lys Glu Ile Tyr Glu Gln Pro Glu Ser Thr Phe 355 360 365 Asn Thr Met Arg Gly Arg Ile Asp Tyr Glu Asn Asn Lys Val Ile Leu 370 375 380 Gly Gly Leu Lys Ala Trp Leu Pro Val Val Arg Arg Ala Arg Arg Leu 385 390 395 400 Ile Met Ile Ala Cys Gly Thr Ser Tyr His Ser Cys Leu Ala Thr Arg 405 410 415 Ala Ile Phe Glu Glu Leu Ser Asp Ile Pro Val Ser Val Glu Leu Ala 420 425 430 Ser Asp Phe Leu Asp Arg Lys Cys Pro Val Phe Arg Asp Asp Val Cys 435 440 445 Val Phe Val Ser Gln Ser Gly Glu Thr Ala Asp Thr Met Leu Ala Leu 450 455 460 Asn Tyr Cys Leu Glu Arg Gly Ala Leu Thr Val Gly Ile Val Asn Ser 465 470 475 480 Val Gly Ser Ser Ile Ser Arg Val Thr His Cys Gly Val His Ile Asn 485 490 495 Ala Gly Pro Glu Ile Gly Val Ala Ser Thr Lys Ala Tyr Thr Ser Gln 500 505 510 Tyr Ile Ala Leu Val Met Phe Ala Leu Ser Leu Ser Asp Asp Arg Val 515 520 525 Ser Lys Ile Asp Arg Arg Ile Glu Ile Ile Gln Gly Leu Lys Leu Ile 530 535 540 Pro Gly Gln Ile Lys Gln Val Leu Lys Leu Glu Pro Arg Ile Lys Lys 545 550 555 560 Leu Cys Ala Thr Glu Leu Lys Asp Gln Lys Ser Leu Leu Leu Leu Gly 565 570 575 Arg Gly Tyr Gln Phe Ala Ala Ala Leu Glu Gly Ala Leu Lys Ile Lys 580 585 590 Glu Ile Ser Tyr Met His Ser Glu Gly Val Leu Ala Gly Glu Leu Lys 595 600 605 His Gly Val Leu Ala Leu Val Asp Glu Asn Leu Pro Ile Ile Ala Phe 610 615 620 Gly Thr Arg Asp Ser Leu Phe Pro Lys Val Val Ser Ser Ile Glu Gln 625 630 635 640 Val Thr Ala Arg Lys Gly His Pro Ile Ile Ile Cys Asn Glu Asn Asp 645 650 655 Glu Val Trp Ala Gln Lys Ser Lys Ser Ile Asp Leu Gln Thr Leu Glu 660 665 670 Val Pro Gln Thr Val Asp Cys Leu Gln Gly Leu Ile Asn Ile Ile Pro 675 680 685 Leu Gln Leu Met Ser Tyr Trp Leu Ala Val Asn Lys Gly Ile Asp Val 690 695 700 Asp Phe Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu 705 710 715 <210> 51 <211> 595 <212> PRT <213> Paramecium bursaria Chlorella virus 1 <220> <223> Amino acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <220> <223> Amino acid sequence of glutamine-fructose-6-phosphate amidotransferase (GFA1) originating from Chlorella virus 1 (PBCV-1) <400> 51 Met Cys Gly Ile Phe Gly Ala Val Ser Asn Asn Asn Ser Ile Glu Val 1 5 10 15 Ser Ile Lys Gly Ile Gln Lys Leu Glu Tyr Arg Gly Tyr Asp Ser Cys 20 25 30 Gly Ile Ala Tyr Thr Asp Gly Gly Ala Ile Glu Arg Ile Arg Ser Ile 35 40 45 Asp Gly Ile Asp Asp Leu Arg Lys Lys Thr Ile Thr Glu Ser Ser Pro 50 55 60 Val Ala Ile Ala His Ser Arg Trp Ser Thr Thr Gly Ile Pro Ser Val 65 70 75 80 Val Asn Ala His Pro His Ile Ser Arg Gly Thr Ser Gly Cys Glu Ser 85 90 95 Arg Ile Ala Val Val His Asn Gly Ile Ile Glu Asn Tyr Gln Gln Ile 100 105 110 Arg Lys Tyr Leu Ile Asn Leu Gly Tyr Thr Phe Asp Ser Gln Thr Asp 115 120 125 Thr Glu Val Ile Ala His Leu Ile Asp Ser Gln Tyr Asn Gly Asn Ile 130 135 140 Leu His Thr Val Gln Met Ala Val Lys His Leu Lys Gly Ser Tyr Ala 145 150 155 160 Ile Ala Val Met Cys His Lys Glu Ser Gly Lys Ile Val Val Ala Lys 165 170 175 Gln Lys Ser Pro Leu Val Leu Gly Ile Gly Ser Asp Gly Ala Tyr Tyr 180 185 190 Ile Ala Ser Asp Val Leu Ala Leu Pro Thr Asn Lys Val Val Tyr Ile 195 200 205 Ser Asp Gly Phe Ser Ala Glu Leu Ser Pro Gly Ser Met Thr Ile Tyr 210 215 220 Asp Leu Asp Gly Asn Lys Val Glu Tyr Glu Val Glu Asp Val Glu Met 225 230 235 240 Glu Gln Thr Ser Met Ser Leu Asp Asn Phe Asp His Tyr Met Ile Lys 245 250 255 Glu Ile Asn Glu Gln Pro Ile Ser Ile Leu Asn Thr Ile Lys Asn Lys 260 265 270 Gly Phe Tyr Ala Glu Ile Phe Gly Asp Leu Ala His Glu Ile Phe Gln 275 280 285 Lys Ile Asp Asn Ile Leu Ile Leu Ala Cys Gly Thr Ser Tyr His Ala 290 295 300 Gly Leu Val Gly Lys Gln Trp Ile Glu Thr Ile Ser Arg Ile Pro Val 305 310 315 320 Asp Val His Ile Ala Ser Glu Tyr Glu Pro Thr Ile Pro Arg Ala Asn 325 330 335 Thr Leu Val Ile Thr Ile Ser Gln Ser Gly Glu Thr Ala Asp Thr Ile 340 345 350 Ala Ala Leu Gln Arg Ala Gln Asn Ala Gly Met Ile Tyr Thr Leu Cys 355 360 365 Ile Cys Asn Ser Pro Lys Ser Thr Leu Val Arg Glu Ser Ile Met Lys 370 375 380 Tyr Ile Thr Lys Cys Gly Ser Glu Val Ser Val Ala Ser Thr Lys Ala 385 390 395 400 Phe Thr Ser Gln Leu Val Val Leu Tyr Met Leu Ala Asn Val Leu Ala 405 410 415 Asn Lys Thr Asp Asp Leu Leu Gly Asp Leu Pro Gln Ala Ile Glu Arg 420 425 430 Val Ile Cys Leu Thr Asn Asp Glu Met Lys Arg Trp Ala Asp Glu Ile 435 440 445 Cys Thr Ala Lys Ser Ala Ile Phe Leu Gly Arg Gly Leu Asn Ala Pro 450 455 460 Val Ala Phe Glu Gly Ala Leu Lys Leu Lys Glu Ile Ser Tyr Ile His 465 470 475 480 Ala Glu Gly Phe Leu Gly Gly Glu Leu Lys His Gly Pro Leu Ala Leu 485 490 495 Leu Asp Asp Lys Ile Pro Val Ile Val Thr Val Ala Asp His Ala Tyr 500 505 510 Leu Asp His Ile Lys Ala Asn Ile Asp Glu Val Leu Ala Arg Asn Val 515 520 525 Thr Val Tyr Ala Ile Val Asp Gln Tyr Val Asn Ile Glu Pro Gln Glu 530 535 540 Arg Leu His Val Val Lys Val Pro Phe Val Ser Lys Glu Phe Ser Pro 545 550 555 560 Ile Ile His Thr Ile Pro Met Gln Leu Leu Ser Tyr Tyr Val Ala Ile 565 570 575 Lys Leu Gly Lys Asn Val Asp Lys Pro Arg Asn Leu Ala Lys Ser Val 580 585 590 Thr Thr Phe 595 <210> 52 <211> 1434 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of UDP-N-acetylglucosamine pyrophosphorylase (QRI1) originating from Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of UDP-N-acetylglucosamine pyrophosphorylase (QRI1) originating from Saccharomyces cerevisiae <400> 52 atgactgaca caaaacagct attcattgaa gccggacaaa gtcaactttt ccacaattgg 60 gaaagcttgt ctcgcaaaga ccaagaagaa ttgctttcaa acctggagca aatatcttcc 120 aagaggtccc ctgcaaaact actggaagac tgtcaaaatg ctattaaatt ctcactagct 180 aactcttcta aggatactgg cgtcgaaatt tcaccattgc cccctacttc gtacgagtcg 240 cttattggca acagtaagaa agaaaatgaa tactggcgtt taggccttga agctattggc 300 aagggtgaag tcgcagtgat tttaatggct ggcggacaag gtacgcggtt aggatcctct 360 caaccaaagg gctgttacga cattggattg ccttctaaga aatctctttt tcaaattcaa 420 gctgaaaagt tgatcaggtt gcaagatatg gtaaaggaca aaaaggtaga aattccttgg 480 tatattatga catcaggccc cactagagcg gctactgagg catactttca agaacacaat 540 tattttggct tgaataaaga acaaattacg ttcttcaacc agggaaccct gcctgccttt 600 gatttaaccg ggaagcattt cctaatgaaa gacccagtaa acctatctca atcaccagat 660 ggaaatggtg gactctaccg tgccatcaag gaaaacaagt tgaacgaaga ctttgatagg 720 agaggaatca agcatgttta catgtactgt gtcgataatg tcctatctaa aatcgcagac 780 cctgtattta ttggttttgc catcaagcat ggcttcgaac tggccaccaa agccgttaga 840 aagagagatg cgcatgaatc agttgggtta attgctacta aaaacgagaa accatgtgtc 900 atagaatatt ctgaaatttc caatgaattg gctgaagcaa aggataaaga tggcttatta 960 aaactacgcg caggcaacat tgtaaatcat tattacctag tggatttact aaaacgtgat 1020 ttggatcagt ggtgtgagaa tatgccatat cacattgcga agaagaaaat tccagcttat 1080 gatagtgtta ccggcaagta cactaagcct accgaaccaa acggtataaa attagagcaa 1140 ttcatatttg atgtctttga cactgtacca ctgaacaagt ttgggtgctt agaagtagat 1200 agatgcaaag aattttcacc tttaaaaaac ggtcctggtt ctaagaacga taatcctgag 1260 accagcagac tagcatattt gaaactagga acctcgtggt tggaagatgc aggcgctatt 1320 gtaaaagatg gggtactagt cgaagtttcc agcaaattga gttatgcagg tgaaaatcta 1380 tcccagttca aaggtaaagt ctttgacaga agtggtatag tattagaaaa ataa 1434 <210> 53 <211> 477 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence of UDP-N-acetylglucosamine pyrophosphorylase (QRI1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence of UDP-N-acetylglucosamine pyrophosphorylase (QRI1) originating from Saccharomyces cerevisiae <400> 53 Met Thr Asp Thr Lys Gln Leu Phe Ile Glu Ala Gly Gln Ser Gln Leu 1 5 10 15 Phe His Asn Trp Glu Ser Leu Ser Arg Lys Asp Gln Glu Glu Leu Leu 20 25 30 Ser Asn Leu Glu Gln Ile Ser Ser Lys Arg Ser Pro Ala Lys Leu Leu 35 40 45 Glu Asp Cys Gln Asn Ala Ile Lys Phe Ser Leu Ala Asn Ser Ser Lys 50 55 60 Asp Thr Gly Val Glu Ile Ser Pro Leu Pro Pro Thr Ser Tyr Glu Ser 65 70 75 80 Leu Ile Gly Asn Ser Lys Lys Glu Asn Glu Tyr Trp Arg Leu Gly Leu 85 90 95 Glu Ala Ile Gly Lys Gly Glu Val Ala Val Ile Leu Met Ala Gly Gly 100 105 110 Gln Gly Thr Arg Leu Gly Ser Ser Gln Pro Lys Gly Cys Tyr Asp Ile 115 120 125 Gly Leu Pro Ser Lys Lys Ser Leu Phe Gln Ile Gln Ala Glu Lys Leu 130 135 140 Ile Arg Leu Gln Asp Met Val Lys Asp Lys Lys Val Glu Ile Pro Trp 145 150 155 160 Tyr Ile Met Thr Ser Gly Pro Thr Arg Ala Ala Thr Glu Ala Tyr Phe 165 170 175 Gln Glu His Asn Tyr Phe Gly Leu Asn Lys Glu Gln Ile Thr Phe Phe 180 185 190 Asn Gln Gly Thr Leu Pro Ala Phe Asp Leu Thr Gly Lys His Phe Leu 195 200 205 Met Lys Asp Pro Val Asn Leu Ser Gln Ser Pro Asp Gly Asn Gly Gly 210 215 220 Leu Tyr Arg Ala Ile Lys Glu Asn Lys Leu Asn Glu Asp Phe Asp Arg 225 230 235 240 Arg Gly Ile Lys His Val Tyr Met Tyr Cys Val Asp Asn Val Leu Ser 245 250 255 Lys Ile Ala Asp Pro Val Phe Ile Gly Phe Ala Ile Lys His Gly Phe 260 265 270 Glu Leu Ala Thr Lys Ala Val Arg Lys Arg Asp Ala His Glu Ser Val 275 280 285 Gly Leu Ile Ala Thr Lys Asn Glu Lys Pro Cys Val Ile Glu Tyr Ser 290 295 300 Glu Ile Ser Asn Glu Leu Ala Glu Ala Lys Asp Lys Asp Gly Leu Leu 305 310 315 320 Lys Leu Arg Ala Gly Asn Ile Val Asn His Tyr Tyr Leu Val Asp Leu 325 330 335 Leu Lys Arg Asp Leu Asp Gln Trp Cys Glu Asn Met Pro Tyr His Ile 340 345 350 Ala Lys Lys Lys Ile Pro Ala Tyr Asp Ser Val Thr Gly Lys Tyr Thr 355 360 365 Lys Pro Thr Glu Pro Asn Gly Ile Lys Leu Glu Gln Phe Ile Phe Asp 370 375 380 Val Phe Asp Thr Val Pro Leu Asn Lys Phe Gly Cys Leu Glu Val Asp 385 390 395 400 Arg Cys Lys Glu Phe Ser Pro Leu Lys Asn Gly Pro Gly Ser Lys Asn 405 410 415 Asp Asn Pro Glu Thr Ser Arg Leu Ala Tyr Leu Lys Leu Gly Thr Ser 420 425 430 Trp Leu Glu Asp Ala Gly Ala Ile Val Lys Asp Gly Val Leu Val Glu 435 440 445 Val Ser Ser Lys Leu Ser Tyr Ala Gly Glu Asn Leu Ser Gln Phe Lys 450 455 460 Gly Lys Val Phe Asp Arg Ser Gly Ile Val Leu Glu Lys 465 470 475 <210> 54 <211> 1713 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of Phosphoglucomutase-1 (PGM1) originating from Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of Phosphoglucomutase-1 (PGM1) originating from Saccharomyces cerevisiae <400> 54 atgtcacttc taatagattc tgtaccaaca gttgcttata aggaccaaaa accgggtact 60 tcaggtttac gtaagaagac caaggttttc atggatgagc ctcattatac tgagaacttc 120 attcaagcaa caatgcaatc tatccctaat ggctcagagg gaaccacttt agttgttgga 180 ggagatggtc gtttctacaa cgatgttatc atgaacaaga ttgccgcagt aggtgctgca 240 aacggtgtca gaaagttagt cattggtcaa ggcggtttac tttcaacacc agctgcttct 300 catataatta gaacatacga ggaaaagtgt accggtggtg gtatcatatt aactgcctca 360 cacaacccag gcggtccaga gaatgattta ggtatcaagt ataatttacc taatggtggg 420 ccagctccag agagtgtcac taacgctatc tgggaagcgt ctaaaaaatt aactcactat 480 aaaattataa agaacttccc caagttgaat ttgaacaagc ttggtaaaaa ccaaaaatat 540 ggcccattgt tagtggacat aattgatcct gccaaagcat acgttcaatt tctgaaggaa 600 atttttgatt ttgacttaat taaaagcttc ttagcgaaac agcgcaaaga caaagggtgg 660 aagttgttgt ttgactcctt aaatggtatt acaggaccat atggtaaggc tatatttgtt 720 gatgaatttg gtttaccggc agaggaagtt cttcaaaatt ggcacccttt acctgatttc 780 ggcggtttac atcccgatcc gaatctaacc tatgcacgaa ctcttgttga cagggttgac 840 cgcgaaaaaa ttgcctttgg agcagcctcc gatggtgatg gtgataggaa tatgatttac 900 ggttatggcc ctgctttcgt ttcgccaggt gattctgttg ccattattgc cgaatatgca 960 cccgaaattc catacttcgc caaacaaggt atttatggct tggcacgttc atttcctaca 1020 tcctcagcca ttgatcgtgt tgcagcaaaa aagggattaa gatgttacga agttccaacc 1080 ggctggaaat tcttctgtgc cttatttgat gctaaaaagc tatcaatctg tggtgaagaa 1140 tccttcggta caggttccaa tcatatcaga gaaaaggacg gtctatgggc cattattgct 1200 tggttaaata tcttggctat ctaccatagg cgtaaccctg aaaaggaagc ttcgatcaaa 1260 actattcagg acgaattttg gaacgagtat ggccgtactt tcttcacaag atacgattac 1320 gaacatatcg aatgcgagca ggccgaaaaa gttgtagctc ttttgagtga atttgtatca 1380 aggccaaacg tttgtggctc ccacttccca gctgatgagt ctttaaccgt tatcgattgt 1440 ggtgattttt cgtatagaga tctagatggc tccatctctg aaaatcaagg ccttttcgta 1500 aagttttcga atgggactaa atttgttttg aggttatccg gcacaggcag ttctggtgca 1560 acaataagat tatacgtaga aaagtatact gataaaaagg agaactatgg ccaaacagct 1620 gacgtcttct tgaaacccgt catcaactcc attgtaaaat tcttaagatt taaagaaatt 1680 ttaggaacag acgaaccaac agtccgcaca tag 1713 <210> 55 <211> 570 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence Phosphoglucomutase-1 (PGM1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence Phosphoglucomutase-1 (PGM1) originating from Saccharomyces cerevisiae <400> 55 Met Ser Leu Leu Ile Asp Ser Val Pro Thr Val Ala Tyr Lys Asp Gln 1 5 10 15 Lys Pro Gly Thr Ser Gly Leu Arg Lys Lys Thr Lys Val Phe Met Asp 20 25 30 Glu Pro His Tyr Thr Glu Asn Phe Ile Gln Ala Thr Met Gln Ser Ile 35 40 45 Pro Asn Gly Ser Glu Gly Thr Thr Leu Val Val Gly Gly Asp Gly Arg 50 55 60 Phe Tyr Asn Asp Val Ile Met Asn Lys Ile Ala Ala Val Gly Ala Ala 65 70 75 80 Asn Gly Val Arg Lys Leu Val Ile Gly Gln Gly Gly Leu Leu Ser Thr 85 90 95 Pro Ala Ala Ser His Ile Ile Arg Thr Tyr Glu Glu Lys Cys Thr Gly 100 105 110 Gly Gly Ile Ile Leu Thr Ala Ser His Asn Pro Gly Gly Pro Glu Asn 115 120 125 Asp Leu Gly Ile Lys Tyr Asn Leu Pro Asn Gly Gly Pro Ala Pro Glu 130 135 140 Ser Val Thr Asn Ala Ile Trp Glu Ala Ser Lys Lys Leu Thr His Tyr 145 150 155 160 Lys Ile Ile Lys Asn Phe Pro Lys Leu Asn Leu Asn Lys Leu Gly Lys 165 170 175 Asn Gln Lys Tyr Gly Pro Leu Leu Val Asp Ile Ile Asp Pro Ala Lys 180 185 190 Ala Tyr Val Gln Phe Leu Lys Glu Ile Phe Asp Phe Asp Leu Ile Lys 195 200 205 Ser Phe Leu Ala Lys Gln Arg Lys Asp Lys Gly Trp Lys Leu Leu Phe 210 215 220 Asp Ser Leu Asn Gly Ile Thr Gly Pro Tyr Gly Lys Ala Ile Phe Val 225 230 235 240 Asp Glu Phe Gly Leu Pro Ala Glu Glu Val Leu Gln Asn Trp His Pro 245 250 255 Leu Pro Asp Phe Gly Gly Leu His Pro Asp Pro Asn Leu Thr Tyr Ala 260 265 270 Arg Thr Leu Val Asp Arg Val Asp Arg Glu Lys Ile Ala Phe Gly Ala 275 280 285 Ala Ser Asp Gly Asp Gly Asp Arg Asn Met Ile Tyr Gly Tyr Gly Pro 290 295 300 Ala Phe Val Ser Pro Gly Asp Ser Val Ala Ile Ile Ala Glu Tyr Ala 305 310 315 320 Pro Glu Ile Pro Tyr Phe Ala Lys Gln Gly Ile Tyr Gly Leu Ala Arg 325 330 335 Ser Phe Pro Thr Ser Ser Ala Ile Asp Arg Val Ala Ala Lys Lys Gly 340 345 350 Leu Arg Cys Tyr Glu Val Pro Thr Gly Trp Lys Phe Phe Cys Ala Leu 355 360 365 Phe Asp Ala Lys Lys Leu Ser Ile Cys Gly Glu Glu Ser Phe Gly Thr 370 375 380 Gly Ser Asn His Ile Arg Glu Lys Asp Gly Leu Trp Ala Ile Ile Ala 385 390 395 400 Trp Leu Asn Ile Leu Ala Ile Tyr His Arg Arg Asn Pro Glu Lys Glu 405 410 415 Ala Ser Ile Lys Thr Ile Gln Asp Glu Phe Trp Asn Glu Tyr Gly Arg 420 425 430 Thr Phe Phe Thr Arg Tyr Asp Tyr Glu His Ile Glu Cys Glu Gln Ala 435 440 445 Glu Lys Val Val Ala Leu Leu Ser Glu Phe Val Ser Arg Pro Asn Val 450 455 460 Cys Gly Ser His Phe Pro Ala Asp Glu Ser Leu Thr Val Ile Asp Cys 465 470 475 480 Gly Asp Phe Ser Tyr Arg Asp Leu Asp Gly Ser Ile Ser Glu Asn Gln 485 490 495 Gly Leu Phe Val Lys Phe Ser Asn Gly Thr Lys Phe Val Leu Arg Leu 500 505 510 Ser Gly Thr Gly Ser Ser Gly Ala Thr Ile Arg Leu Tyr Val Glu Lys 515 520 525 Tyr Thr Asp Lys Lys Glu Asn Tyr Gly Gln Thr Ala Asp Val Phe Leu 530 535 540 Lys Pro Val Ile Asn Ser Ile Val Lys Phe Leu Arg Phe Lys Glu Ile 545 550 555 560 Leu Gly Thr Asp Glu Pro Thr Val Arg Thr 565 570 <210> 56 <211> 1500 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of UTP--glucose-1-phosphate uridylyltransferase (UGP1) originating from <220> <223> Nucleic acid sequence of UTP--glucose-1-phosphate uridylyltransferase (UGP1) originating from <400> 56 atgtccacta agaagcacac caaaacacat tccacttatg cattcgagag caacacaaac 60 agcgttgctg cctcacaaat gagaaacgcc ttaaacaagt tggcggactc tagtaaactt 120 gacgatgctg ctcgcgctaa gtttgagaac gaactggatt cgtttttcac gcttttcagg 180 agatatttgg tagagaagtc ttctagaacc accttggaat gggacaagat caagtctccc 240 aacccggatg aagtggttaa gtatgaaatt atttctcagc agcccgagaa tgtctcaaac 300 ctttccaaat tggctgtttt gaagttgaac ggtgggctgg gtacctccat gggctgcgtt 360 ggccctaaat ctgttattga agtgagagag ggaaacacct ttttggattt gtctgttcgt 420 caaattgaat acttgaacag acagtacgat agcgacgtgc cattgttatt gatgaattct 480 ttcaacactg acaaggatac ggaacacttg attaagaagt attccgctaa cagaatcaga 540 atcagatctt tcaatcaatc caggttccca agagtctaca aggattcttt attgcctgtc 600 cccaccgaat acgattctcc actggatgct tggtatccac caggtcacgg tgatttgttt 660 gaatctttac acgtatctgg tgaactggat gccttaattg cccaaggaag agaaatatta 720 tttgtttcta acggtgacaa cttgggtgct accgtcgact taaaaatttt aaaccacatg 780 atcgagactg gtgccgaata tataatggaa ttgactgata agaccagagc cgatgttaaa 840 ggtggtactt tgatttctta cgatggtcaa gtccgtttat tggaagtcgc ccaagttcca 900 aaagaacaca ttgacgaatt caaaaatatc agaaagttta ccaacttcaa cacgaataac 960 ttatggatca atctgaaagc agtaaagagg ttgatcgaat cgagcaattt ggagatggaa 1020 atcattccaa accaaaaaac tataacaaga gacggtcatg aaattaatgt cttacaatta 1080 gaaaccgctt gtggtgctgc tatcaggcat tttgatggtg ctcacggtgt tgtcgttcca 1140 agatcaagat tcttgcctgt caagacctgt tccgatttgt tgctggttaa atcagatcta 1200 ttccgtctgg aacacggttc tttgaagtta gacccatccc gttttggtcc aaacccatta 1260 atcaagttgg gctcgcattt caaaaaggtt tctggtttta acgcaagaat ccctcacatc 1320 ccaaaaatcg tcgagctaga tcatttgacc atcactggta acgtcttttt aggtaaagat 1380 gtcactttga ggggtactgt catcatcgtt tgctccgacg gtcataaaat cgatattcca 1440 aacggctcca tattggaaaa tgttgtcgtt actggtaatt tgcaaatctt ggaacattga 1500 <210> 57 <211> 499 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence of UTP--glucose-1-phosphate uridylyltransferase (UGP1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence of UTP--glucose-1-phosphate uridylyltransferase (UGP1) originating from Saccharomyces cerevisiae <400> 57 Met Ser Thr Lys Lys His Thr Lys Thr His Ser Thr Tyr Ala Phe Glu 1 5 10 15 Ser Asn Thr Asn Ser Val Ala Ala Ser Gln Met Arg Asn Ala Leu Asn 20 25 30 Lys Leu Ala Asp Ser Ser Lys Leu Asp Asp Ala Ala Arg Ala Lys Phe 35 40 45 Glu Asn Glu Leu Asp Ser Phe Phe Thr Leu Phe Arg Arg Tyr Leu Val 50 55 60 Glu Lys Ser Ser Arg Thr Thr Leu Glu Trp Asp Lys Ile Lys Ser Pro 65 70 75 80 Asn Pro Asp Glu Val Val Lys Tyr Glu Ile Ile Ser Gln Gln Pro Glu 85 90 95 Asn Val Ser Asn Leu Ser Lys Leu Ala Val Leu Lys Leu Asn Gly Gly 100 105 110 Leu Gly Thr Ser Met Gly Cys Val Gly Pro Lys Ser Val Ile Glu Val 115 120 125 Arg Glu Gly Asn Thr Phe Leu Asp Leu Ser Val Arg Gln Ile Glu Tyr 130 135 140 Leu Asn Arg Gln Tyr Asp Ser Asp Val Pro Leu Leu Leu Met Asn Ser 145 150 155 160 Phe Asn Thr Asp Lys Asp Thr Glu His Leu Ile Lys Lys Tyr Ser Ala 165 170 175 Asn Arg Ile Arg Ile Arg Ser Phe Asn Gln Ser Arg Phe Pro Arg Val 180 185 190 Tyr Lys Asp Ser Leu Leu Pro Val Pro Thr Glu Tyr Asp Ser Pro Leu 195 200 205 Asp Ala Trp Tyr Pro Pro Gly His Gly Asp Leu Phe Glu Ser Leu His 210 215 220 Val Ser Gly Glu Leu Asp Ala Leu Ile Ala Gln Gly Arg Glu Ile Leu 225 230 235 240 Phe Val Ser Asn Gly Asp Asn Leu Gly Ala Thr Val Asp Leu Lys Ile 245 250 255 Leu Asn His Met Ile Glu Thr Gly Ala Glu Tyr Ile Met Glu Leu Thr 260 265 270 Asp Lys Thr Arg Ala Asp Val Lys Gly Gly Thr Leu Ile Ser Tyr Asp 275 280 285 Gly Gln Val Arg Leu Leu Glu Val Ala Gln Val Pro Lys Glu His Ile 290 295 300 Asp Glu Phe Lys Asn Ile Arg Lys Phe Thr Asn Phe Asn Thr Asn Asn 305 310 315 320 Leu Trp Ile Asn Leu Lys Ala Val Lys Arg Leu Ile Glu Ser Ser Asn 325 330 335 Leu Glu Met Glu Ile Ile Pro Asn Gln Lys Thr Ile Thr Arg Asp Gly 340 345 350 His Glu Ile Asn Val Leu Gln Leu Glu Thr Ala Cys Gly Ala Ala Ile 355 360 365 Arg His Phe Asp Gly Ala His Gly Val Val Val Pro Arg Ser Arg Phe 370 375 380 Leu Pro Val Lys Thr Cys Ser Asp Leu Leu Leu Val Lys Ser Asp Leu 385 390 395 400 Phe Arg Leu Glu His Gly Ser Leu Lys Leu Asp Pro Ser Arg Phe Gly 405 410 415 Pro Asn Pro Leu Ile Lys Leu Gly Ser His Phe Lys Lys Val Ser Gly 420 425 430 Phe Asn Ala Arg Ile Pro His Ile Pro Lys Ile Val Glu Leu Asp His 435 440 445 Leu Thr Ile Thr Gly Asn Val Phe Leu Gly Lys Asp Val Thr Leu Arg 450 455 460 Gly Thr Val Ile Ile Val Cys Ser Asp Gly His Lys Ile Asp Ile Pro 465 470 475 480 Asn Gly Ser Ile Leu Glu Asn Val Val Val Thr Gly Asn Leu Gln Ile 485 490 495 Leu Glu His <210> 58 <211> 480 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of Glucosamine 6-phosphate N-acetyltransferase (GNA1) originating from Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of Glucosamine 6-phosphate N-acetyltransferase (GNA1) originating from Saccharomyces cerevisiae <400> 58 atgagcttac ccgatggatt ttatataagg cgaatggaag agggggattt ggaacaggtc 60 actgagacgc taaaggtttt gaccaccgtg ggcactatta cccccgaatc cttcagcaaa 120 ctcataaaat actggaatga agccacagta tggaatgata acgaagataa aaaaataatg 180 caatataacc ccatggtgat tgtggacaag cgcaccgaga cggttgccgc tacggggaat 240 atcatcatcg aaagaaagat cattcatgaa ctggggctat gtggccacat cgaggacatt 300 gcagtaaact ccaagtatca gggccaaggt ttgggcaagc tcttgattga tcaattggta 360 actatcggct ttgactacgg ttgttataag attattttag attgcgatga gaaaaatgtc 420 aaattctatg aaaaatgtgg gtttagcaac gcaggcgtgg aaatgcaaat tagaaaatag 480 <210> 59 <211> 159 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence of Glucosamine 6-phosphate N-acetyltransferase (GNA1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence of Glucosamine 6-phosphate N-acetyltransferase (GNA1) originating from Saccharomyces cerevisiae <400> 59 Met Ser Leu Pro Asp Gly Phe Tyr Ile Arg Arg Met Glu Glu Gly Asp 1 5 10 15 Leu Glu Gln Val Thr Glu Thr Leu Lys Val Leu Thr Thr Val Gly Thr 20 25 30 Ile Thr Pro Glu Ser Phe Ser Lys Leu Ile Lys Tyr Trp Asn Glu Ala 35 40 45 Thr Val Trp Asn Asp Asn Glu Asp Lys Lys Ile Met Gln Tyr Asn Pro 50 55 60 Met Val Ile Val Asp Lys Arg Thr Glu Thr Val Ala Ala Thr Gly Asn 65 70 75 80 Ile Ile Ile Glu Arg Lys Ile Ile His Glu Leu Gly Leu Cys Gly His 85 90 95 Ile Glu Asp Ile Ala Val Asn Ser Lys Tyr Gln Gly Gln Gly Leu Gly 100 105 110 Lys Leu Leu Ile Asp Gln Leu Val Thr Ile Gly Phe Asp Tyr Gly Cys 115 120 125 Tyr Lys Ile Ile Leu Asp Cys Asp Glu Lys Asn Val Lys Phe Tyr Glu 130 135 140 Lys Cys Gly Phe Ser Asn Ala Gly Val Glu Met Gln Ile Arg Lys 145 150 155 <210> 60 <211> 1674 <212> DNA <213> Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of phosphoacetylglucosamine mutase (PCM1) originating from Saccharomyces cerevisiae <220> <223> Nucleic acid sequence of phosphoacetylglucosamine mutase (PCM1) originating from Saccharomyces cerevisiae <400> 60 atgaaggttg attacgagca attgtgcaaa ctctacgatg acacgtgccg cacaaagaat 60 gtgcagttca gttacggtac ggccggattc agaacgctgg ccaagaattt ggatacggtg 120 atgttcagta ctggtatact ggcggttctc aggtcgctga agcttcaggg tcagtatgtg 180 ggggtgatga tcacggcgtc gcacaaccca taccaggaca acggggtcaa gatcgtggaa 240 ccagacggat cgatgctttt ggccacatgg gagccatatg ccatgcagtt ggccaatgcg 300 gcctcttttg ccactaattt tgaagaattt cgtgttgagt tggccaagct gattgaacac 360 gaaaagattg atttgaatac aaccgtcgtg cctcacatcg tggttgggag agactctagg 420 gaaagtagtc catacttgct gcgctgcttg acttcctcca tggccagcgt cttccacgcg 480 caagttttgg acctaggctg tgtcactacg cctcaattgc attacattac tgatttgtcc 540 aacaggcgga aactggaagg agacacagcg ccagttgcca cagaacagga ctactattcg 600 ttctttatag gagccttcaa cgagctcttc gccacgtatc agctggagaa gaggctgtct 660 gtcccaaaat tgttcataga cacagccaat ggtatcggtg gtccacagtt gaaaaaacta 720 ctggcctccg aagattggga cgtgccagcg gagcaagttg aggtaatcaa cgacaggtcc 780 gatgttccag aactgttgaa ttttgaatgc ggtgcggatt atgtgaagac taaccagaga 840 ttacccaagg gtctttctcc atcctcgttt gattcgctat attgctcctt tgatggtgac 900 gcagacaggg ttgtgttcta ctatgtcgac tcaggatcaa aatttcattt gttggatggt 960 gacaaaattt ccactttgtt tgcaaagttc ttgtctaaac aactagaatt ggcacaccta 1020 gaacattctt tgaagattgg tgttgtgcaa actgcctatg caaacggcag ttccaccgct 1080 tacataaaaa atacgttgca ctgtcccgtg tcttgcacta agacaggtgt taaacacttg 1140 catcatgaag ctgccactca gtacgatatt ggcatttatt tcgaagcaaa tggacatggt 1200 acgattatat tcagcgaaaa atttcatcga actatcaaat ctgaattatc caagtccaag 1260 ttaaatggtg atacgttagc tttgagaact ttgaagtgtt tctctgaatt gattaatcag 1320 accgtgggag atgctatttc agacatgctt gctgtccttg ctactttggc gattttgaaa 1380 atgtcgccaa tggattggga tgaagagtat actgatttgc ccaacaagct ggttaagtgc 1440 atcgttcctg ataggtcaat tttccaaacc acggaccagg aaagaaaatt gctcaatcca 1500 gtggggttgc aagacaagat agatcttgtg gtagccaagt atcccatggg aagaagcttt 1560 gtcagagcca gtggtacgga ggatgcggtg agggtttatg cggaatgtaa ggactcctct 1620 aagttaggtc aattttgtga cgaagtggtg gagcacgtta aggcatctgc ttga 1674 <210> 61 <211> 557 <212> PRT <213> Saccharomyces cerevisiae <220> <223> Amino acid sequence of phosphoacetylglucosamine mutase (PCM1) originating from Saccharomyces cerevisiae <220> <223> Amino acid sequence of phosphoacetylglucosamine mutase (PCM1) originating from Saccharomyces cerevisiae <400> 61 Met Lys Val Asp Tyr Glu Gln Leu Cys Lys Leu Tyr Asp Asp Thr Cys 1 5 10 15 Arg Thr Lys Asn Val Gln Phe Ser Tyr Gly Thr Ala Gly Phe Arg Thr 20 25 30 Leu Ala Lys Asn Leu Asp Thr Val Met Phe Ser Thr Gly Ile Leu Ala 35 40 45 Val Leu Arg Ser Leu Lys Leu Gln Gly Gln Tyr Val Gly Val Met Ile 50 55 60 Thr Ala Ser His Asn Pro Tyr Gln Asp Asn Gly Val Lys Ile Val Glu 65 70 75 80 Pro Asp Gly Ser Met Leu Leu Ala Thr Trp Glu Pro Tyr Ala Met Gln 85 90 95 Leu Ala Asn Ala Ala Ser Phe Ala Thr Asn Phe Glu Glu Phe Arg Val 100 105 110 Glu Leu Ala Lys Leu Ile Glu His Glu Lys Ile Asp Leu Asn Thr Thr 115 120 125 Val Val Pro His Ile Val Val Gly Arg Asp Ser Arg Glu Ser Ser Pro 130 135 140 Tyr Leu Leu Arg Cys Leu Thr Ser Ser Met Ala Ser Val Phe His Ala 145 150 155 160 Gln Val Leu Asp Leu Gly Cys Val Thr Thr Pro Gln Leu His Tyr Ile 165 170 175 Thr Asp Leu Ser Asn Arg Arg Lys Leu Glu Gly Asp Thr Ala Pro Val 180 185 190 Ala Thr Glu Gln Asp Tyr Tyr Ser Phe Phe Ile Gly Ala Phe Asn Glu 195 200 205 Leu Phe Ala Thr Tyr Gln Leu Glu Lys Arg Leu Ser Val Pro Lys Leu 210 215 220 Phe Ile Asp Thr Ala Asn Gly Ile Gly Gly Pro Gln Leu Lys Lys Leu 225 230 235 240 Leu Ala Ser Glu Asp Trp Asp Val Pro Ala Glu Gln Val Glu Val Ile 245 250 255 Asn Asp Arg Ser Asp Val Pro Glu Leu Leu Asn Phe Glu Cys Gly Ala 260 265 270 Asp Tyr Val Lys Thr Asn Gln Arg Leu Pro Lys Gly Leu Ser Pro Ser 275 280 285 Ser Phe Asp Ser Leu Tyr Cys Ser Phe Asp Gly Asp Ala Asp Arg Val 290 295 300 Val Phe Tyr Tyr Val Asp Ser Gly Ser Lys Phe His Leu Leu Asp Gly 305 310 315 320 Asp Lys Ile Ser Thr Leu Phe Ala Lys Phe Leu Ser Lys Gln Leu Glu 325 330 335 Leu Ala His Leu Glu His Ser Leu Lys Ile Gly Val Val Gln Thr Ala 340 345 350 Tyr Ala Asn Gly Ser Ser Thr Ala Tyr Ile Lys Asn Thr Leu His Cys 355 360 365 Pro Val Ser Cys Thr Lys Thr Gly Val Lys His Leu His His Glu Ala 370 375 380 Ala Thr Gln Tyr Asp Ile Gly Ile Tyr Phe Glu Ala Asn Gly His Gly 385 390 395 400 Thr Ile Ile Phe Ser Glu Lys Phe His Arg Thr Ile Lys Ser Glu Leu 405 410 415 Ser Lys Ser Lys Leu Asn Gly Asp Thr Leu Ala Leu Arg Thr Leu Lys 420 425 430 Cys Phe Ser Glu Leu Ile Asn Gln Thr Val Gly Asp Ala Ile Ser Asp 435 440 445 Met Leu Ala Val Leu Ala Thr Leu Ala Ile Leu Lys Met Ser Pro Met 450 455 460 Asp Trp Asp Glu Glu Tyr Thr Asp Leu Pro Asn Lys Leu Val Lys Cys 465 470 475 480 Ile Val Pro Asp Arg Ser Ile Phe Gln Thr Thr Asp Gln Glu Arg Lys 485 490 495 Leu Leu Asn Pro Val Gly Leu Gln Asp Lys Ile Asp Leu Val Val Ala 500 505 510 Lys Tyr Pro Met Gly Arg Ser Phe Val Arg Ala Ser Gly Thr Glu Asp 515 520 525 Ala Val Arg Val Tyr Ala Glu Cys Lys Asp Ser Ser Lys Leu Gly Gln 530 535 540 Phe Cys Asp Glu Val Val Glu His Val Lys Ala Ser Ala 545 550 555 <210> 62 <211> 574 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pTDH3 <220> <223> Nucleic acid sequence of promotor pTDH3 <400> 62 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt 60 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt 120 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc 180 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa 240 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg 300 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc 360 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta 420 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct 480 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa 540 cttagtttcg aataaacaca cataaacaaa caaa 574 <210> 63 <211> 585 <212> DNA <213> Saccharomyces kudriavzevii <220> <223> Nucleic acid sequence of promotor pTDH3.Sk <220> <223> Nucleic acid sequence of promotor pTDH3.Sk <400> 63 caattcatcg gcccttttag cggctacccg cgccatctaa atgatagggc gggtgacact 60 atggtaaatc ccataattag gtgtctgggt gagtggttct gatgccggca tccactaaat 120 atattggagc ccatttttta cgcgggcttc cagaaaaaaa gagaatccca gcaccaaaag 180 gtggttctct tcaccaacca tcagatcata ggtccacaac cacacataac aggggcacaa 240 aaaggcaaaa aacggacata acctcaatgg agtgatgcaa attgactgga gcaaaagctg 300 acacaaggca ttgattgacc tacgcatgta tctgtattct tttcttacac cttctattac 360 cttctaactc tttgggttgg aaaaaactga aaaaaaaggt tgggacctgg ttcccccaag 420 ttgtccccct acttggttat taaatatata aagacagcaa gtgttgatta taatcttgta 480 aatctatagt tcttaatcta tacttctatt tatattttaa attagtcttt ttatttccaa 540 gtccccaaga acttagtttc gaataaacac acacaaataa acaca 585 <210> 64 <211> 700 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of promotor pTDH3-1.sba <220> <223> Nucleic acid sequence of promotor pTDH3-1.sba <400> 64 gcagcgcttc ttccgctcta gtttttatag ttattattac taccacctta aaaatacgta 60 aatactcaaa atagtagtga tattcccaac cttattcatc caaggcacat catcatcatc 120 agccattcat ctttcacctg ccattagtaa cccgtcttct cattgagcgg gttacggcag 180 ccacaggcca cattccgaat gtctgggtga gcggtccctt ttccagcatc cactaaatat 240 ctccgatccc gctttttaat ctggcttcct gaaaaaaaga gaatcccagc accaaaaaat 300 ggctctcttc accaaccatc agatcatagg tcccattctc ttaccgcaac cgtacagaac 360 aggggaaaac gggtacaacc tcaatggagt gatgcaaact gactggagca aaaagctgac 420 acaaggcaat cgacctacgt gtctgtctat tttctcacac cttctattac cttctaactc 480 tctgggttgg aaaaaactga aaaaaaggtt gagaccagtt tccacaaatc atccccctgt 540 ttgattaata aatatataaa gacgacaact atcgatcata aactcataaa actataactc 600 ctttacactt cttattttat agttattcta ttttaattct tattgatttt aaaaccccaa 660 gaacttagtt tcgaaaacac acacacacaa acaattaaaa 700 <210> 65 <211> 602 <212> DNA <213> Saccharomyces arboricola <220> <223> Nucleic acid sequence of promotor pTDH3.Sar <220> <223> Nucleic acid sequence of promotor pTDH3.Sar <400> 65 gagctaaata tcagcccttc gggtcctgcc tgctacccgg tcctgttcga ataaaaacgc 60 gggtaacacg acccagtaac acctgtcgtt gggtgtctgg gtcagaagtt ctgataccgg 120 cttccactaa atagattggg ttccgctctt tacgctggct tcctgaaaaa agagattccg 180 ggcaccaaaa aattggtctc tttgccaacc atcagatcat aggtccattc tcttaccata 240 accacacagg ataggggcac cacaggcgaa aatgggcaca aaatctcaat ggagtgatgc 300 aaattagctg gaacaaaagc tgacacaagg caattaacct gcgcatgtat ccatctcctt 360 ttcttacacc ttctcttacg ttctaactgt ttgggttgga aaaattaaaa aaaaaaggtt 420 gagaccagtt tccccaaatc gtccccctac ttgattcata aatatataaa gacgacaact 480 attgattata atcttgtaaa tctataactc tttactttct cctatttata atttaactta 540 atctttttag atttaaaacc ccaagaactt agtttcgaac aaacacacac aaataaacaa 600 aa 602 <210> 66 <211> 550 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pENO2 <220> <223> Nucleic acid sequence of promotor pENO2 <400> 66 cgctcagcat ctgcttcttc ccaaagatga acgcggcgtt atgtcactaa cgacgtgcac 60 caacttgcgg aaagtggaat cccgttccaa aactggcatc cactaattga tacatctaca 120 caccgcacgc cttttttctg aagcccactt tcgtggactt tgccatatgc aaaattcatg 180 aagtgtgata ccaagtcagc atacacctca ctagggtagt ttctttggtt gtattgatca 240 tttggttcat cgtggttcat taattttttt tctccattgc tttctggctt tgatcttact 300 atcatttgga tttttgtcga aggttgtaga attgtatgtg acaagtggca ccaagcatat 360 ataaaaaaaa aaagcattat cttcctacca gagttgattg ttaaaaacgt atttatagca 420 aacgcaattg taattaattc ttattttgta tcttttcttc ccttgtctca atcttttatt 480 tttattttat ttttcttttc ttagtttctt tcataacacc aagcaactaa tactataaca 540 tacaataata 550 <210> 67 <211> 598 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pTEF3 <220> <223> Nucleic acid sequence of promotor pTEF3 <400> 67 ggctgataat agcgtataaa caatgcatac tttgtacgtt caaaatacaa tgcagtagat 60 atatttatgc atattacata taatacatat cacataggaa gcaacaggcg cgttggactt 120 ttaattttcg aggaccgcga atccttacat cacacccaat cccccacaag tgatccccca 180 cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc 240 cgcgcatcgc cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc 300 ttcctctagg gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc 360 ctcgtttctt tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt 420 gaaaattttt ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa 480 taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt 540 ttacttcttg ctcattagaa agaaagcata gcaatctaat ctaagtttta attacaaa 598 <210> 68 <211> 383 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pTEF1 <220> <223> Nucleic acid sequence of promotor pTEF1 <400> 68 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc 60 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt 120 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg 180 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca 240 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg 300 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctacgata cagttctcac 360 atcacatccg aacataaaca acc 383 <210> 69 <211> 383 <212> DNA <213> Ashbya gossypii <220> <223> Nucleic acid sequence of promotor pTEF1.ago <220> <223> Nucleic acid sequence of promotor pTEF1.ago <400> 69 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc 60 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt 120 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg 180 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca 240 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg 300 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctacgata cagttctcac 360 atcacatccg aacataaaca acc 383 <210> 70 <211> 498 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of promotor pTEF1.Sba <220> <223> Nucleic acid sequence of promotor pTEF1.Sba <400> 70 cgccaacaaa ccttcgaaca ctttaatttt cgaggaccgc agatcctcac atcacaccca 60 cacccaagac tgcttccccc acacaccctg catctgtaca ctttcttctg ctctgttttt 120 ctctccggcg ttctctcggg tcgcccgcat cgccgcgccg gctggaaccg cccacgcacc 180 gcatattgca aatcgcctgc cccctcttgc tcctttttga gggcgcgccg ttacccgcgc 240 ccagggtccg gaaaaagaaa caaggctcta ccgcgtttct ttttccttgt cgaaaaaggc 300 aaaaatgaaa atttttatca cgtttctttt tttttgaaaa attttttttt tggttttttt 360 tctttcgatg gcctcccatt gatatttaag ttaataaatg gttttcagtt ttcaagtttc 420 agtttgtgtt cttctttgct aactttcact tacacctcga aagaaagtat agcaatctaa 480 tcttagtttt aattacaa 498 <210> 71 <211> 76 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pPDC1 <220> <223> Nucleic acid sequence of promotor pPDC1 <400> 71 ttatttacct atctctaaac ttcaacacct tatatcataa ctaatatttc ttgagataag 60 cacactgcac ccatac 76 <210> 72 <211> 998 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pCCW12 <220> <223> Nucleic acid sequence of promotor pCCW12 <400> 72 aaccagggca aagcaaaata aaagaaactt aatacgttat gccgtaatga agggctacca 60 aaaacgataa tctcaactgt aaacaggtac aatgcggacc cttttgccac aaaacataca 120 tcattcattg ccggaaaaag aaagaagtga agacagcagt gcagccagcc atgttgcgcc 180 aatctaatta tagatgctgg tgccctgagg atgtatctgg agccagccat ggcatcatgc 240 gctaccgccg gatgtaaaat ccgacacgca aaagaaaacc ttcgaggttg cgcacttcgc 300 ccacccatga accacacggt tagtccaaaa ggggcagttc agattccaga tgcgggaatt 360 agcttgctgc caccctcacc tcactaacgc tgcggtgtgc ggatacttca tgctatttat 420 agacgcgcgt gtcggaatca gcacgcgcaa gaaccaaatg ggaaaatcgg aatgggtcca 480 gaactgcttt gagtgctggc tattggcgtc tgatttccgt tttgggaatc ctttgccgcg 540 cgcccctctc aaaactccgc acaagtccca gaaagcggga aagaaataaa acgccaccaa 600 aaaaaaaaat aaaagccaat cctcgaagcg tgggtggtag gccctggatt atcccgtaca 660 agtatttctc aggagtaaaa aaaccgtttg ttttggaatt ccccatttcg cggccaccta 720 cgccgctatc tttgcaacaa ctatctgcga taactcagca aattttgcat attcgtgttg 780 cagtattgcg ataatgggag tcttacttcc aacataacgg cagaaagaaa tgtgagaaaa 840 ttttgcatcc tttgcctccg ttcaagtata taaagtcggc atgcttgata atctttcttt 900 ccatcctaca ttgttctaat tattcttatt ctcctttatt ctttcctaac ataccaagaa 960 attaatcttc tgtcattcgc ttaaacacta tatcaata 998 <210> 73 <211> 976 <212> DNA <213> Saccharomyces mikatae <220> <223> Nucleic acid sequence of promotor pCCW12.Sm <220> <223> Nucleic acid sequence of promotor pCCW12.Sm <400> 73 cgttgtgctg tagtgaagga agactaaaaa ggataatcac agttgtaaag aggtataatg 60 cggacccttt tgccacaaaa cacacatctt tcgtttccaa aataagaaag aagaaagcaa 120 aaagattagc agccacgttg ctgcgatcta attatagacg gtggcgtcat cattctcacc 180 caagattgtg tcttgaacct gccacgggtc ctgcgttatc gccggatgta aaactagaca 240 tgcaaaaaaa ggaccttcca ggtagcgtgc tccacaccac ccatgaccac cacagttagt 300 ccaaaagagg cagcaccact tcccgatggg ggaattagat tgctgccacc ctcacctcac 360 taatgctgcg gtgtgcggat atgccctgct atatatagct ccgcgttttt gaaccagcac 420 agcgcgagca ccaaaaagga aaatcgcata ggcccagaac tgatttcagc acgggctatt 480 ggcgttgggt ttccgttctg ggaaaccttc gccgcgtccc cctcacaaac ctccgcacaa 540 gttcgagcaa gcgggaaaaa acgaaaaacg ccattaatac taaataaagc aaatcctcga 600 agcgtgggtg gcaagcccct ggatttttcc gcacaagtac tcttctcagg agtaaaaaaa 660 cccgtttgtt ttggaattcc ccatttcgcg gccatctacg ccggtatctt cgcaatatct 720 atcagcgata actcagcaat tttaatattc gtgttgcagt gctgcgatag cgggagtctt 780 gtttgtaaca taacggcaga aaggaatgag agaaaatttt ccattctttg gcctccgttc 840 aagtatataa agccggcatg cttggtaatc tttctctctc ttctgtattg tttctataat 900 acttttatct tctaattatt ttctgaaaaa accaagaaat taatcttctg tcattcgctt 960 aaacactata tcaata 976 <210> 74 <211> 750 <212> DNA <213> Saccharomyces kudriavzevii <220> <223> Nucleic acid sequence of promotor pCCW12.Sk <220> <223> Nucleic acid sequence of promotor pCCW12.Sk <400> 74 tgtaaaatcc tacacgcaaa aaacctcttg ggttgcgcgt tttccaccac ccacgacccg 60 cacaatcaat ccaaaagggg caacgccagt tcccaatgcg ggaattagct cctcaccccc 120 ctcacccgct aacgctgcgg tgtgcggaca cgcagcagta tttatagatc ctcgtgttgg 180 aaccagcccg cgtgagcacc aaattggaaa gtcgcaatgg gcccagaacc gctttcagta 240 ctgggccatt ggcgtctagt ttccgttttg agcgtccttc gccgcgtctc tctgtgaaat 300 ccccgcacaa gtctgagcag gcaaaaaaaa aaaacgccac caaaaataaa taaagccaat 360 cctcgaagga tgagtaggaa aggaagcccc tggttttttc ccgcacgaat atttttcagg 420 agtgaaaaaa tccgtttgtt ttggaattcc ccatttcgcg ctcacctacg ccggtatctt 480 tggaacaact atcagcgata actcagcaaa atttgcatat ccgtgctgca atagtgcgat 540 agtgggattg ggagtcttgt tgcatcataa cggcagaaag gaatgaataa aaattttccg 600 ttctttgtct ccgttcaagt atataaagtc ggcatgcttg attatctttt cttctcttct 660 actacatttc tatttcactt tctactctat tcttccctga aaaacccaag aaaataatct 720 tctgtcattc gcttaaacaa tatatcaaaa 750 <210> 75 <211> 750 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of promotor pCCW12.Sba <220> <223> Nucleic acid sequence of promotor pCCW12.Sba <400> 75 accatgccac ggtgctggcc ccacttccac ccacgacccg cacggttggc ccgaaagggg 60 caacaccagt tcccaatacg gaaattagtc cgccaccacc ctcacctcgc tagagctgcg 120 gtgtgcgggc gtgcatcgct atttatagac atgcctgctg gcgtcaccgc gcgcgagcac 180 caaacaggaa aatcgcactg ggcccagaac cacgctatgc gctgggccga tggcgtccgg 240 tttccctttg ggagccccct gccacgttcg cctaacaaat ccccgcaccg gcttgagaaa 300 aaagcgaaaa gcgaaaaaaa aaaatcaacg ccaccaaaat taaaaaaaaa gagccatcct 360 cgaagggtga atagtagccc ctgacttttc ccgcacagac agacaccttt caggagtgaa 420 caaaaaagca gtttgttttg gaattccccc atttcgcggt ggcctgcgca ggtatctctg 480 cgtcaactat cagcgataac tcagcaaatt ttgcatattc gtgttgcgat actacgataa 540 tgggagtctg tcgcctaata acggcaacaa ggaatgagag agaaaaattt tcttcattct 600 ccagctcccg ttcaagtata taaggtcggc atggtcgatt gtctttcctt ctcttcagtt 660 acgtctctct atttacatta ttcttatttt tatttaataa aaaccccaag aaattaatct 720 tctgtcattc gcttaaacac tatatcaaaa 750 <210> 76 <211> 750 <212> DNA <213> Saccharomyces arboricola <220> <223> Nucleic acid sequence of promotor pCCW12.Sar <220> <223> Nucleic acid sequence of promotor pCCW12.Sar <400> 76 aaaaaacaac cttctgccaa cctgcgtgct tctcaccacc catgacccac acaattgacc 60 cgaatggggc aactccagtt cccaatacgg gaattaactc gccaccatat ttaccgcgtt 120 gaagctgtgg tgtgcggaca ctccgtacta tttatagacc cacgcggtgg aaccagcacg 180 cgcgcgcact aaacaggaaa atcgcattga gtccagaacc gccaccagca cttggccatt 240 ggcgtctaat ttccgttttc ggcgcccctc accgcgtcct tctaacaaag cgcgcacaag 300 cttgagcaag tgaaaagaaa attaaaaata aaaaaccgcc accaaaacaa ataaagcaat 360 cttcgaagtg tgggttggtg ggaagcccct ggcttttccc gcaccagtcg ttttcaggag 420 taaaaaaata cccgtttgtt ttggaattcc ccatttcgcg gcgacctgcg ccggtatctt 480 tgcaacaact atttgcgata actcagcaaa atttgcatat tcgtgttggg atattgcgat 540 agtgggagtc ttgttgcata ataacggtaa aaagaagtga aggaaaaaaa tttgcatcct 600 ttagtctcag ttcaagtata taaagtcggg atattcaatt atctttcttt ctcttgctca 660 aaggtttcta tatttttttt atagtatttc ttttgttata aaataccaag aaattaatct 720 tctgtcattc gcttaaatac tacatcaata 750 <210> 77 <211> 500 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pNUP57 <220> <223> Nucleic acid sequence of promotor pNUP57 <400> 77 tcatctgcgc aatgactatc aagaccttct gcaagaattt caaatctcac tgaaaatctt 60 gaccgaaaag tgtcttgaaa acccatcaag cctgcaaaac ctatctttga cattagtctc 120 cattataaaa acggcatagt tgggagaaaa ctttccatac ttcaattgtg gactgatata 180 agtattttag ttttgcccgc atgatcatcc cacatggcta cagcagttct ctcataggaa 240 aatagtacaa tagctacgtg atataatcta aataattgtt gccaatgtgt aattatatca 300 ttttgaacgt acgcgaaatg gattattttc aaaaattttg tttcttgaaa tgagtaaaag 360 caaaagtcca actctccaag tcgatgtaaa caactttttg ccaaagggac tgaaagacta 420 aatcgaggat tatcccgttc aaactattcc agaaacgctc gttagtaaca aaagacatac 480 cttgttgacc aattgatcac 500 <210> 78 <211> 523 <212> DNA <213> Ashbya gossypii <220> <223> Nucleic acid sequence of promotor pCCW10.ago <220> <223> Nucleic acid sequence of promotor pCCW10.ago <400> 78 ggtaccacgg caacctcgtt cgctgttcat ccccttcgtc acacaggacg ttggatgccg 60 taagcagcgt tgcttttgat cctcaggatc ggccgggtaa cccgcggctg cttctatttt 120 agtattcata tctcaagcac atccattccg gccgtttggg ggcgccgccg cactcgtgtc 180 cattcctacc gtggcactta gggctatcct gtcggagcgc cccgccgacc gccttatcgg 240 caccaaaagt agaagccccg gccccgcgtg gctcagactc accatcggtg ctatttactt 300 ttcgatcaga tcgcggcgcg cggtggccgg catttccgga agcggccacg gagcagaggt 360 ggcgcattcg aatcgcatac gtcttcgcca cgccggaaaa aaaattttcg gctatataag 420 gagaggcggc cgtcttgctg caggcagttt cactttctct aaaaccaaag aacatcgatt 480 tctttagtca ctcgcttcct tacaccgaac tcgaggcggc cgc 523 <210> 79 <211> 943 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pCWP2 <220> <223> Nucleic acid sequence of promotor pCWP2 <400> 79 ctagcctccc ctttttattt tgtgcggtca ccgcaaggga caaagctttt cttagaaaac 60 cgtctgagaa gcataacgta cgccatcccc tagacatatt aataatgcta cagatactat 120 gctgctcgtc tttttttgac gaccctttta ttgcaatgtg caactaatgg caaacaacca 180 catagtatca cagtattaca ttgcctccac cgatgcggat gttagggcgc caagtctgtc 240 atgaagcatg ttcctgtcat aatcttgtat gcaaaatacc gcgttctgcg ccactgatat 300 gctaggcagc agcaacctat gcagaagatt gcttttccca cgcctgtttt acgtctccag 360 ggcacttgaa acaatgcagc gatcgccgcc acaacacgcc aaagagaagc gaaagtgggc 420 ctgggcggcc tcagtttcgg cagaggtaaa caacacgaac tgaactgcct tagctccgaa 480 gggcaattcc acaggcactc cgcggggccc ggccaaggcc caaaaggcgt ggaatatgcg 540 cgttttgggg ccataacacc cagtaccacg gccggaacgg gccatataat aagtttttca 600 ctctcaagaa tggtaaacgt aaataggaac atcccactac cctagaaatt gcggaaattt 660 cgcgcttatc attagaaaat ctggaaccgt cctttttcct ctttcttgca tttccctttc 720 cgtattattg ccattcttta actgcatttg gggaaccgta gaccaaaagc caaacagaga 780 aatgtaacgt tctaaaaaaa aaacaacgaa aaaattgaaa aataagatac aataatcgta 840 tataaatcag gcttcttgtt catcattttc aattctcttc ttgccatccc ttttcctatc 900 tttgttcttt tcttctcata atcaagaata aataacttca tca 943 <210> 80 <211> 535 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pRPLA1 <220> <223> Nucleic acid sequence of promotor pRPLA1 <400> 80 tcaagttgga tactgatctg atctctccgc cctactacca gggaccctca tgattaccgc 60 tcgaatgcga cgtttcctgc ctcataaaac tggcttgaaa atatttattc gctgaacagt 120 agcctagctt ataaaaattt catttaatta atgtaatatg aaaactcaca tgccttctgt 180 ttctaaaatt gtcacagcaa gaaataacat taccatacgt gatcttatta aactctagta 240 tcttgtctaa tacttcattt aaaagaagcc ttaaccctgt agcctcatct atgtctgcta 300 catatcgtga ggtacgaata tcgtaagatg ataccacgca actttgtaat gatttttttt 360 ttttcatttt ttaaagaatg cctttacatg gtatttgaaa aaaatatctt tataaagttt 420 gcgatctctt ctgttctgaa taatttttag taaaagaaat caaaagaata aagaaatagt 480 ccgctttgtc caatacaaca gcttaaaccg attatctcta aaataacaag aagaa 535 <210> 81 <211> 505 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pCUP1 <220> <223> Nucleic acid sequence of promotor pCUP1 <400> 81 cggcaaactt caacgatttc tatgatgcat tttataatta gtaagccgat cccattaccg 60 acatttgggc gctatacgtg catatgttca tgtatgtatc tgtatttaaa acacttttgt 120 attatttttc ctcatatatg tgtataggtt tatacggatg atttaattat tacttcacca 180 ccctttattt caggctgata tcttagcctt gttactagtt agaaaaagac atttttgctg 240 tcagtcactg tcaagagatt cttttgctgg catttcttct agaagcaaaa agagcgatgc 300 gtcttttccg ctgaaccgtt ccagcaaaaa agactaccaa cgcaatatgg attgtcagaa 360 tcatataaaa gagaagcaaa taactccttg tcttgtatca attgcattat aatatcttct 420 tgttagtgca atatcatata gaagtcatcg aaatagatat taagaaaaac aaactgtaca 480 atcaatcaat caatcatcac ataaa 505 <210> 82 <211> 510 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pMET6 <220> <223> Nucleic acid sequence of promotor pMET6 <400> 82 ccacaggaaa tatttcacgt gacttacaaa cagagtcgta cgtcaggacc ggagtcaggt 60 gaaaaaatgt gggccggtaa agggaaaaaa ccagaaacgg gactactatc gaactcgttt 120 agtcgcgaac gtgcaaaagg ccaatatttt tcgctagagt catcgcagtc atggcagctc 180 tttcgctcta tctcccggtc gcaaaactgt ggtagtcata gctcgttctg ctcaattgag 240 aactgtgaat gtgaatatgg aacaaatgcg atagatgcac taatttaagg gaagctagct 300 agttttccca actgcgaaag aaaaaaagga aagaaaaaaa aattctatat aagtgataga 360 tatttccatc tttactagca ttagtttctc ttttacgtat tcaatatttt tgttaaactc 420 ttcctttatc ataaaaaagc aagcatctaa gagcattgac aacactctaa gaaacaaaat 480 accaatataa tttcaaagta catatcaaaa 510 <210> 83 <211> 686 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pMET25 <220> <223> Nucleic acid sequence of promotor pMET25 <400> 83 ttacattatc aatccttgcg tttcagcttc cactaattta gatgactatt tctcatcatt 60 tgcgtcatct tctaacaccg tatatgataa tatactagta acgtaaatac tagttagtag 120 atgatagttg atttttattc caacactaag aaataatttc gccatttctt gaatgtattt 180 aaagatattt aatgctataa tagacattta aatccaattc ttccaacata caatgggagt 240 ttggccgagt ggtttaaggc gtcagattta ggtggattta acctctaaaa tctctgatat 300 cttcggatgc aagggttcga atcccttagc tctcattatt ttttgctttt tctcttgagg 360 tcacatgatc gcaaaatggc aaatggcacg tgaagctgtc gatattgggg aactgtggtg 420 gttggcaaat gactaattaa gttagtcaag gcgccatcct catgaaaact gtgtaacata 480 ataaccgaag tgtcgaaaag gtggcacctt gtccaattga acacgctcga tgaaaaaaat 540 aagatatata taaggttaag taaagcgtct gttagaaagg aagtttttcc tttttcttgc 600 tctcttgtct tttcatctac tatttccttc gtgtaataca gggtcgtcag atacatagat 660 acaattctat tacccccatc cataca 686 <210> 84 <211> 363 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of promotor pSAM1 <220> <223> Nucleic acid sequence of promotor pSAM1 <400> 84 gaaacggacg taagacggaa atagaatttg aagataaagt tatatatcac tacacacgaa 60 tactttcttt tttttttttc acaggaaaac tgtggtggcg cccttgccta ctagtgcatt 120 tcttttttcg ggttcttgtc tcgacgaaat tttagcctca tcgtagtttt tcactctggt 180 atcgatgaaa aagggaagag taaaaagttt tccgtttagt acttaatggg attggtttgg 240 gacgtatata tcgactggtg ttgtctgtta ttcatcgttg tttttcggtt agcttcgaaa 300 aaaaaataga gtaaaaacca ggaatttacc ctaaaaacaa gaaaaaataa gataaacgaa 360 aat 363 <210> 85 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tTPI1 <220> <223> Nucleic acid sequence of terminator tTPI1 <400> 85 gattaatata attatataaa aatattatct tcttttcttt atatctagtg ttatgtaaaa 60 taaattgatg actacggaaa gcttttttat attgtttctt tttcattctg agccacttaa 120 atttcgtgaa tgttcttgta agggacggta gatttacaag tgatacaaca aaaagcaagg 180 cgctttttct aataaaaaga agaaaagcat ttaacaattg aacacctcta tatcaacgaa 240 gaatattact ttgtctctaa atccttgtaa aatgtgtacg atctctatat gggttactca 300 <210> 86 <211> 299 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tMET25 <220> <223> Nucleic acid sequence of terminator tMET25 <400> 86 gtgtgcgtaa tgagttgtaa aattatgtat aaacctactt tctctcacaa gtactatact 60 tttataaaac gaactttatt gaaatgaata tccttttttt cccttgttac atgtcgtgac 120 tcgtactttg aacctaaatt gttctaacat caaagaacag tgttaattcg cagtcgagaa 180 gaaaaatatg gtgaacaaga ctcatctact tcatgagact actttacgcc tcctataaag 240 ctgtcacact ggataaattt attgtaggac caagttacaa aagaggatga tggaggttt 299 <210> 87 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tDIT1 <220> <223> Nucleic acid sequence of terminator tDIT1 <400> 87 taaagtaaga gcgctacatt ggtctacctt tttgttcttt tacttaaaca ttagttagtt 60 cgttttcttt ttctcatttt tttatgtttc ccccccaaag ttctgatttt ataatatttt 120 atttcacaca attccattta acagaggggg aatagattct ttagcttaga aaattagtga 180 tcaatatata tttgcctttc ttttcatctt ttcagtgata ttaatggttt cgagacactg 240 caatggccct agttgtctaa gaggatagat gttactgtca aagatgatat tttgaatttc 300 <210> 88 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tRPL3 <220> <223> Nucleic acid sequence of terminator tRPL3 <400> 88 gaagttttgt tagaaaataa atcatttttt aattgagcat tcttattcct attttattta 60 aatagtttta tgtattgtta gctacataca acagtttaaa tcaaattttc tttttcccaa 120 gtccaaaatg gaggtttatt ttgatgaccc gcatgcgatt atgttttgaa agtataagac 180 tacatacatg tacatatatt taaacatgta aacccgtcca ttatattgct tactttcttc 240 ttttttgccg ttttgacttg gacctctggt ttgctatttc cttacaatct ttgctacaat 300 <210> 89 <211> 304 <212> DNA <213> Saccharomyces mikatae <220> <223> Nucleic acid sequence of terminator tRPL3.sm <220> <223> Nucleic acid sequence of terminator tRPL3.sm <400> 89 gaagttttta aagcattttt tagacacttc tcatttttct aagttttttt aaaatagttt 60 tatgtattta ctacgtatca caatttgaaa taattcatct tcccaaaaaa ctaagatttt 120 tatccttgtc acgatccgta accagtttat aatattttag agcttataca cgtacgtata 180 cacacgtgtc ggtacatgag aattacgttc aaaattattc actttttttt tctctgccgt 240 tttacttttg aactctgtct cgctatttcc ttacaatctt cgctacaata ccacttgccc 300 ttgg 304 <210> 90 <211> 299 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of terminator tRPL3.sba <220> <223> Nucleic acid sequence of terminator tRPL3.sba <400> 90 gaagttttct gacaaaaaca taacgttttt tccaatcatt tcttattttt ccggtttatt 60 taaatagttt ttatgtacta ttatacgtat gactatttaa cttaaattct tcctcccaag 120 aaatctccca agtttttcat tatcatggca tacaccacta tcagttacaa aatggtagct 180 caaccatata tatatctcta tatacacata taaatgcaaa caggtccaag tcaccgctca 240 ctgcagtttc ttttgccgtt ttgacttcga tctctgcttg gctattttct cacaatcct 299 <210> 91 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tRPL41B <220> <223> Nucleic acid sequence of terminator tRPL41B <400> 91 gcggattgag agcaaatcgt taagttcagg tcaagtaaaa attgatttcg aaaactaatt 60 tctcttatac aatcctttga ttggaccgtc atcctttcga atataagatt ttgttaagaa 120 tattttagac agagatctac tttatattta atatctagat attacataat ttcctctcta 180 ataaaatatc attaataaaa taaaaatgaa gcgatttgat tttgtgttgt caacttagtt 240 tgccgctatg cctcttgggt aatgctatta ttgaatcgaa gggctttatt atattaccct 300 <210> 92 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tRPL15A <220> <223> Nucleic acid sequence of terminator tRPL15A <400> 92 gctggttgat ggaaaatata attttattgg gcaaactttt gtttatctga tgtgttttat 60 actattatct ttttaattaa tgattctata tacaaacctg tatatttttt ctttaaccaa 120 tttttttttt tatagaccta gagctgtact tttattctgc tatcaagcaa acccctaccc 180 cctcttctca atcctcccct caggcagaac ttatctacct gtatcaagga gcggacgagg 240 gagtcctaat tgttctacgt ataccaatgc tagcagctta cataggtggt ggcactacca 300 <210> 93 <211> 300 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of terminator tRPL15A.sba <220> <223> Nucleic acid sequence of terminator tRPL15A.sba <400> 93 gctggctgat gaaaaatata gttctgttgg gcaagcattt gtttacctag catctctttt 60 atactattat tatctttata tttgatgatt ttatatacaa gttgtatacc ttttctttaa 120 ccaatttttt tttttctaat ggtgcaccta gaagtacatt ttttctcacc aatagatagt 180 caagatactc ccagcctcta tggcgttacc acggagccga caagggaaag ctgcttatct 240 tacatatgca gatgccaagg cccgttacag gccgccttat tgatgtttag aaatagcttc 300 <210> 94 <211> 300 <212> DNA <213> Artificial Sequence <220> <223> Nucleic acid sequence of terminator tIDP1 <220> <223> Nucleic acid sequence of terminator tIDP1 <400> 94 tcgaatttac gtagcccaat ctaccacttt tttttttcat tttttaaagt gttatactta 60 gttatgctct aggataatga actacttttt tttttttttt tttactgtta tcataaatat 120 atatacctta ttgttgtttg caaccgtcgg ttaattcctt atcaaggttc cccaagttcg 180 gatcattacc atcaatttcc aacattttca tgagttcttc ttcttcatta ccgtgtttta 240 gggggctgtt cgcacttcta atagggctat caccaagctg ttctaattcg tccaaaagtt 300 <210> 95 <211> 300 <212> DNA <213> Saccharomyces bayanus <220> <223> Nucleic acid sequence of terminator tTEF1.sba <220> <223> Nucleic acid sequence of terminator tTEF1.sba <400> 95 ggagattgat aggacttttc tagttgcata tcttttattt ttaaatctta tctattagtt 60 aattttttgt aatttatcct tatatatata gtttggttat tctaaaacat catttcagta 120 tctaaaacct ctcttattca ttaccttttt atttaatggt ttttgctaca ggcaaaaatt 180 taatggtttt tgctacaggc aaaaatcccg ccgtggactt attccacgtt aactcggtta 240 cagggtcatg aaccattttg tcaattatcg aaataacttc ttcaaaagtc cctcttactt 300 <210> 96 <211> 1113 <212> DNA <213> Saccharomyces cerevisiae <220> <223> nucleic acid sequence of Glutamine synthetase (GLN1) <220> <223> nucleic acid sequence of Glutamine synthetase (GLN1) <400> 96 atggctgaag caagcatcga aaagactcaa attttacaaa aatatctaga actggaccaa 60 agaggtagaa taattgccga atacgtttgg atcgatggta ctggtaactt acgttccaaa 120 ggtagaactt tgaagaagag aatcacatcc attgaccaat tgccagaatg gaacttcgac 180 ggttcttcta ccaaccaagc gccaggccac gactctgaca tctatttgaa acccgttgct 240 tactacccag atcccttcag gagaggtgac aacattgttg tcttggccgc atgttacaac 300 aatgacggta ctccaaacaa gttcaaccac agacacgaag ctgccaagct atttgctgct 360 cataaggatg aagaaatctg gtttggtcta gaacaagaat acactctatt tgacatgtat 420 gacgatgttt acggatggcc aaagggtggg tacccagctc cacaaggtcc ttactactgt 480 ggtgttggtg ccggtaaggt ttatgccaga gacatgatcg aagctcacta cagagcttgt 540 ttgtatgccg gattagaaat ttctggtatt aacgctgaag tcatgccatc tcaatgggaa 600 ttccaagtcg gtccatgtac cggtattgac atgggtgacc aattatggat ggccagatac 660 tttttgcaca gagtggcaga agagtttggt atcaagatct cattccatcc aaagccattg 720 aagggtgact ggaacggtgc cggttgtcac actaacgttt ccaccaagga aatgagacaa 780 ccaggtggta tgaaatacat cgaacaagcc atcgagaagt tatccaagag acacgctgaa 840 cacattaagt tgtacggtag cgataacgac atgagattaa ctggtagaca tgaaaccgct 900 tccatgactg ccttttcttc tggtgtcgcc aacagaggta gctcaattag aatcccaaga 960 tccgtcgcca aggaaggtta cggttacttt gaagaccgta gaccagcttc caacatcgac 1020 ccatacttgg ttacaggtat catgtgtgaa actgtttgcg gtgctattga caatgctgac 1080 atgacgaagg aatttgaaag agaatcttca taa 1113 <210> 97 <211> 370 <212> PRT <213> Saccharomyces cerevisiae <220> <223> amino acid sequence of Glutamine synthetase (GLN1) <220> <223> amino acid sequence of Glutamine synthetase (GLN1) <400> 97 Met Ala Glu Ala Ser Ile Glu Lys Thr Gln Ile Leu Gln Lys Tyr Leu 1 5 10 15 Glu Leu Asp Gln Arg Gly Arg Ile Ile Ala Glu Tyr Val Trp Ile Asp 20 25 30 Gly Thr Gly Asn Leu Arg Ser Lys Gly Arg Thr Leu Lys Lys Arg Ile 35 40 45 Thr Ser Ile Asp Gln Leu Pro Glu Trp Asn Phe Asp Gly Ser Ser Thr 50 55 60 Asn Gln Ala Pro Gly His Asp Ser Asp Ile Tyr Leu Lys Pro Val Ala 65 70 75 80 Tyr Tyr Pro Asp Pro Phe Arg Arg Gly Asp Asn Ile Val Val Leu Ala 85 90 95 Ala Cys Tyr Asn Asn Asp Gly Thr Pro Asn Lys Phe Asn His Arg His 100 105 110 Glu Ala Ala Lys Leu Phe Ala Ala His Lys Asp Glu Glu Ile Trp Phe 115 120 125 Gly Leu Glu Gln Glu Tyr Thr Leu Phe Asp Met Tyr Asp Asp Val Tyr 130 135 140 Gly Trp Pro Lys Gly Gly Tyr Pro Ala Pro Gln Gly Pro Tyr Tyr Cys 145 150 155 160 Gly Val Gly Ala Gly Lys Val Tyr Ala Arg Asp Met Ile Glu Ala His 165 170 175 Tyr Arg Ala Cys Leu Tyr Ala Gly Leu Glu Ile Ser Gly Ile Asn Ala 180 185 190 Glu Val Met Pro Ser Gln Trp Glu Phe Gln Val Gly Pro Cys Thr Gly 195 200 205 Ile Asp Met Gly Asp Gln Leu Trp Met Ala Arg Tyr Phe Leu His Arg 210 215 220 Val Ala Glu Glu Phe Gly Ile Lys Ile Ser Phe His Pro Lys Pro Leu 225 230 235 240 Lys Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Val Ser Thr Lys 245 250 255 Glu Met Arg Gln Pro Gly Gly Met Lys Tyr Ile Glu Gln Ala Ile Glu 260 265 270 Lys Leu Ser Lys Arg His Ala Glu His Ile Lys Leu Tyr Gly Ser Asp 275 280 285 Asn Asp Met Arg Leu Thr Gly Arg His Glu Thr Ala Ser Met Thr Ala 290 295 300 Phe Ser Ser Gly Val Ala Asn Arg Gly Ser Ser Ile Arg Ile Pro Arg 305 310 315 320 Ser Val Ala Lys Glu Gly Tyr Gly Tyr Phe Glu Asp Arg Arg Pro Ala 325 330 335 Ser Asn Ile Asp Pro Tyr Leu Val Thr Gly Ile Met Cys Glu Thr Val 340 345 350 Cys Gly Ala Ile Asp Asn Ala Asp Met Thr Lys Glu Phe Glu Arg Glu 355 360 365 Ser Ser 370 <210> 98 <211> 6438 <212> DNA <213> Saccharomyces cerevisiae <220> <223> nucleic acid sequence of Glutamate synthase (GLT1) <220> <223> nucleic acid sequence of Glutamate synthase (GLT1) <400> 98 atgccagtgt tgaaatcaga caatttcgat ccattggaag aagcttacga aggtgggaca 60 attcaaaact ataacgatga acaccatctt cataaatctt gggcaaatgt gattccggac 120 aaacgaggac tttacgaccc tgattatgaa catgacgctt gtggtgtcgg tttcgtagca 180 aataagcatg gtgaacagtc tcacaagatt gttactgacg ctagatatct tttagtgaat 240 atgacacatc gtggtgccgt ctcatctgat gggaacggtg acggtgccgg tattctgcta 300 ggtattcctc acgaatttat gaaaagagaa ttcaagttag atcttgatct agacatacct 360 gagatgggca aatacgccgt aggtaacgtc ttcttcaaga agaacgaaaa aaataacaag 420 aaaaatttaa ttaagtgtca gaagattttc gaggatttag ctgcatcctt caacttatcc 480 gtattaggtt ggagaaacgt ccccgtagat tctactattt taggagacgt tgcattatct 540 cgtgaaccta ctattctaca gccattattg gttccattgt atgatgaaaa acaaccggag 600 tttaatgaaa ctaaatttag aactcaattg tatcttttaa ggaaggaggc ctctcttcaa 660 ataggactgg aaaactggtt ctatgtttgt tccctaaaca ataccaccat tgtttacaag 720 ggtcaattga cgccagctca agtgtataac tactatcccg acttgactaa tgcgcatttc 780 aaatcccaca tggcgttggt ccattcaaga ttttccacta atactttccc ctcttgggat 840 agagctcaac ctttacgttg gctagctcat aatggtgaaa ttaacacctt aagaggtaac 900 aagaattgga tgcgctccag agaaggtgtg atgaattcag caactttcaa agatgagtta 960 gacaaactat acccaattat cgaagaaggt ggttctgatt cagctgcatt ggataacgtt 1020 ttagaactat tgactattaa tggcacatta tctctacctg aagctgttat gatgatggtt 1080 cctgaagcgt atcataagga tatggattct gacctaaaag catggtacga ctgggctgca 1140 tgtctgatgg aaccttggga tggtccagct ttgttaactt tcactgatgg acgttactgt 1200 ggtgctatat tggatagaaa tggtttaaga ccttgtcgtt attacatcac tagtgatgac 1260 agagttatct gtgcttcaga ggtaggtgtc attcctatcg aaaattcatt ggttgttcaa 1320 aaaggtaaac tgaagccagg tgatttattc ctagtggata ctcaattggg tgaaatggtc 1380 gatactaaaa agttaaaatc tcaaatctca aaaagacaag attttaagtc ttggttatcc 1440 aaagtcatca agttagacga cttgttatca aaaaccgcta atttggttcc taaagaattt 1500 atatcacagg attcattgtc tttgaaagtt caaagtgacc cacgtctatt ggccaatggt 1560 tataccttcg aacaagtcac atttctgtta actccaatgg ctttaacagg taaagaagct 1620 ttaggttcga tgggtaacga tgcgccactg gcttgtttaa atgaaaatcc tgtcttactt 1680 tatgattatt tcagacaatt gtttgctcaa gtgaccaatc ctccaattga cccaattcgt 1740 gaagcaaatg ttatgtcgtt agaatgttat gtcggacctc aaggcaacct tttggaaatg 1800 cattcatctc aatgtgatcg tttattattg aaatctccta ttttgcattg gaatgagttc 1860 caagctttga aaaacattga agctgcttac ccatcatggt ctgtagcaga aattgatatc 1920 acattcgaca agagtgaggg tctattgggc tataccgaca caattgataa aatcactaag 1980 ttagcgagcg aagcaattga tgatggtaaa aagatcttaa taattactga caggaaaatg 2040 ggtgccaacc gtgtttccat ctcctctttg attgcaattt catgtattca tcatcaccta 2100 atcagaaaca agcagcgttc ccaagttgct ttgattttgg aaacaggtga agccagagaa 2160 attcaccatt tctgtgtcct actaggttat ggttgtgatg gtgtttatcc atacttagcc 2220 atggaaactt tggtcagaat gaatagagaa ggtctacttc gtaatgtcaa caatgacaat 2280 gatacacttg aggaagggca aatactagaa aattacaagc acgctattga tgcaggtatc 2340 ttgaaggtta tgtctaaaat gggtatctcc actctagcat cctacaaagg tgctcaaatt 2400 tttgaagccc taggtttaga taactctatt gttgatttgt gtttcacagg tacttcttcc 2460 agaattagag gtgtaacttt cgagtatttg gctcaagatg ccttttcttt acatgagcgt 2520 ggttatccat ccagacaaac cattagtaaa tctgttaact taccagaaag tggtgaatac 2580 cactttaggg atggtggtta caaacacgtc aacgaaccaa ccgcaattgc ttcgttacaa 2640 gatactgtca gaaacaaaaa tgatgtctct tggcaattat atgtaaagaa ggaaatggaa 2700 gcaattagag actgtacact aagaggactg ttagaattag attttgaaaa ttctgtcagt 2760 atccctctag aacaagttga accatggact gaaattgcca gaagatttgc gtcaggtgca 2820 atgtcttatg gttctatttc tatggaagct cactctacat tggctattgc catgaatcgt 2880 ttaggggcca aatccaattg tggtgaaggt ggtgaagacg cagaacgttc tgctgttcaa 2940 gaaaacggtg atactatgag atctgctatc aaacaagttg cttccgctag attcggtgta 3000 acttcatact acttgtcaga tgctgatgaa atccaaatta agattgctca gggtgctaag 3060 ccgggtgaag gtggtgaact accagcccac aaagtgtcta aggatatcgc aaaaaccagg 3120 cactccaccc ctaatgttgg gttaatctct cctcctcctc atcacgatat ttattccatt 3180 gaagatttga aacaactgat ttatgatttg aaatgtgcta atccaagagc gggaatttct 3240 gtaaagttgg tttccgaagt tggtgttggt attgttgcct ctggtgtagc taaggctaaa 3300 gccgatcata tcttagtttc tggtcatgat ggtggtacag gtgctgcaag atggacgagt 3360 gtcaaatatg cgggtttgcc atgggaatta ggtctagctg aaactcacca gactttagtc 3420 ttgaatgatt taagacgtaa tgttgttgtc caaaccgatg gtcaattgag aactgggttt 3480 gatattgctg ttgcagtttt attaggggca gaatctttta ccttggcaac agttccatta 3540 attgctatgg gttgtgttat gttaagaaga tgtcacttga actcttgtgc tgttggtatt 3600 gccacacaag atccatattt gagaagtaag tttaagggtc agcccgaaca tgttatcaac 3660 ttcttctatt acttgatcca agatttaaga caaatcatgg ccaagttagg attccgtacc 3720 attgacgaaa tggtgggtca ttctgaaaaa ttaaagaaaa gggacgacgt aaatgccaaa 3780 gccataaata tcgatttatc tcctattttg accccagcac atgttattcg tccaggtgtt 3840 ccaaccaagt tcactaagaa acaagaccac aaactccaca cccgtctaga taataagtta 3900 atcgatgagg ctgaagttac tttggatcgt ggcttaccag tgaatattga cgcctctata 3960 atcaatactg atcgtgcact cggttctact ttatcttaca gagtctcgaa gaaatttggt 4020 gaagatggtt tgccaaagga caccgttgtc gttaacatag aaggttcagc gggtcaatct 4080 tttggtgctt tcctagcttc tggtatcact tttatcttga atggtgatgc taatgattat 4140 gttggtaaag gtttatccgg tggtattatt gtcattaaac caccaaagga ttctaaattc 4200 aagagtgatg aaaatgtaat tgttggtaac acttgtttct atggtgctac ttctggtact 4260 gcattcattt caggtagtgc cggtgagcgt ttcggtgtca gaaactctgg tgccaccatc 4320 gttgttgaga gaattaaggg taacaatgcc tttgagtata tgactggtgg tcgtgccatt 4380 gtcttatcac aaatggaatc cctaaacgcc ttctctggtg ctactggtgg tattgcatac 4440 tgtttaactt ccgattacga cgattttgtt ggaaagatta acaaagatac tgttgagtta 4500 gaatcattat gtgacccggt cgagattgcg tttgttaaga atttgatcca ggagcattgg 4560 aactacacac aatctgatct agcagccagg attctcggta atttcaacca ttatttgaaa 4620 gatttcgtta aagtcattcc aactgattat aagaaagttt tgttgaagga gaaagcagaa 4680 gctgccaagg caaaggctaa ggcaacttca gaatacttaa agaagtttag atcgaaccaa 4740 gaagttgatg acgaagtcaa tactctattg attgctaatc aaaaagctaa agagcaagaa 4800 aaaaagaaga gtattactat ttcaaataag gccactttga aggagcctaa ggttgttgat 4860 ttagaagatg cagttccaga ttccaaacag ctagagaaga atagcgaaag gattgaaaaa 4920 acacgtggtt ttatgatcca caaacgtcgt catgagacac acagagatcc aagaaccaga 4980 gttaatgact ggaaagaatt tactaaccct attaccaaga aggatgccaa atatcaaact 5040 gcgagatgta tggattgtgg tacaccattc tgtttatctg ataccggttg tcccctatct 5100 aacattatcc ccaagtttaa tgaattgtta ttcaagaacc aatggaagtt ggcactggac 5160 aaattgctag agacaaacaa tttcccagaa ttcactggaa gagtatgtcc agcaccctgt 5220 gagggagctt gtacactagg tattattgaa gacccagtcg gcataaaatc ggttgaaaga 5280 attatcattg acaatgcttt caaggaagga tggattaagc cttgtccacc aagtacacgc 5340 actggcttta cagtgggtgt cattggttct ggtccagcag gtttagcgtg tgctgatatg 5400 ttgaaccgtg ccggacatac ggtcactgtt tatgaaagat ccgaccgttg tggtgggtta 5460 ttgatgtatg gtattccaaa catgaagttg gataaggcta tagtgcaacg tcgtattgat 5520 ctattgagtg ccgaaggtat tgactttgtt accaacaccg aaattggtaa aaccataagc 5580 atggatgagc taaagaacaa gcacaatgca gtagtgtatg ctatcggttc taccattcca 5640 cgtgacttac ctattaaggg tcgtgaattg aagaatattg attttgccat gcagttgttg 5700 gaatctaaca caaaagcttt attgaacaaa gatctggaaa tcattcgtga aaagatccaa 5760 ggtaagaaag taattgttgt cggtggtggt gacacaggta acgattgttt aggtacatct 5820 gtaagacacg gtgcagcatc agttttgaat ttcgaattgt tgcctgagcc accagtggaa 5880 cgtgccaaag acaatccatg gcctcaatgg ccgcgtgtca tgagagtgga ctacggtcat 5940 gctgaagtga aagagcatta tggtagagac cctcgtgaat actgcatctt gtccaaggaa 6000 tttatcggta acgatgaggg tgaagtcact gccatcagaa ctgtgcgcgt agaatggaag 6060 aagtcacaaa gtggcgtatg gcaaatggta gaaattccca acagtgaaga gatctttgaa 6120 gccgatatca ttttgttgtc tatgggtttc gtgggtcctg aattgatcaa tggcaacgat 6180 aacgaagtta agaagacaag acgtggtacg attgccacac tcgacgactc ctcatactct 6240 attgatggag gaaagacttt tgcatgtggt gactgtagaa gagggcaatc tttgattgtc 6300 tgggccatcc aagaaggtag aaaatgtgct gcctctgtcg ataagttcct aatggacggc 6360 actacgtatc taccaagtaa tggtggtatc gttcaacgtg attacaaact attgaaagaa 6420 ttagctagtc aagtctaa 6438 <210> 99 <211> 2145 <212> PRT <213> Saccharomyces cerevisiae <220> <223> amino acid sequence of Glutamate synthase (GLT1) <220> <223> amino acid sequence of Glutamate synthase (GLT1) <400> 99 Met Pro Val Leu Lys Ser Asp Asn Phe Asp Pro Leu Glu Glu Ala Tyr 1 5 10 15 Glu Gly Gly Thr Ile Gln Asn Tyr Asn Asp Glu His His Leu His Lys 20 25 30 Ser Trp Ala Asn Val Ile Pro Asp Lys Arg Gly Leu Tyr Asp Pro Asp 35 40 45 Tyr Glu His Asp Ala Cys Gly Val Gly Phe Val Ala Asn Lys His Gly 50 55 60 Glu Gln Ser His Lys Ile Val Thr Asp Ala Arg Tyr Leu Leu Val Asn 65 70 75 80 Met Thr His Arg Gly Ala Val Ser Ser Asp Gly Asn Gly Asp Gly Ala 85 90 95 Gly Ile Leu Leu Gly Ile Pro His Glu Phe Met Lys Arg Glu Phe Lys 100 105 110 Leu Asp Leu Asp Leu Asp Ile Pro Glu Met Gly Lys Tyr Ala Val Gly 115 120 125 Asn Val Phe Phe Lys Lys Asn Glu Lys Asn Asn Lys Lys Asn Leu Ile 130 135 140 Lys Cys Gln Lys Ile Phe Glu Asp Leu Ala Ala Ser Phe Asn Leu Ser 145 150 155 160 Val Leu Gly Trp Arg Asn Val Pro Val Asp Ser Thr Ile Leu Gly Asp 165 170 175 Val Ala Leu Ser Arg Glu Pro Thr Ile Leu Gln Pro Leu Leu Val Pro 180 185 190 Leu Tyr Asp Glu Lys Gln Pro Glu Phe Asn Glu Thr Lys Phe Arg Thr 195 200 205 Gln Leu Tyr Leu Leu Arg Lys Glu Ala Ser Leu Gln Ile Gly Leu Glu 210 215 220 Asn Trp Phe Tyr Val Cys Ser Leu Asn Asn Thr Thr Ile Val Tyr Lys 225 230 235 240 Gly Gln Leu Thr Pro Ala Gln Val Tyr Asn Tyr Tyr Pro Asp Leu Thr 245 250 255 Asn Ala His Phe Lys Ser His Met Ala Leu Val His Ser Arg Phe Ser 260 265 270 Thr Asn Thr Phe Pro Ser Trp Asp Arg Ala Gln Pro Leu Arg Trp Leu 275 280 285 Ala His Asn Gly Glu Ile Asn Thr Leu Arg Gly Asn Lys Asn Trp Met 290 295 300 Arg Ser Arg Glu Gly Val Met Asn Ser Ala Thr Phe Lys Asp Glu Leu 305 310 315 320 Asp Lys Leu Tyr Pro Ile Ile Glu Glu Gly Gly Ser Asp Ser Ala Ala 325 330 335 Leu Asp Asn Val Leu Glu Leu Leu Thr Ile Asn Gly Thr Leu Ser Leu 340 345 350 Pro Glu Ala Val Met Met Met Val Pro Glu Ala Tyr His Lys Asp Met 355 360 365 Asp Ser Asp Leu Lys Ala Trp Tyr Asp Trp Ala Ala Cys Leu Met Glu 370 375 380 Pro Trp Asp Gly Pro Ala Leu Leu Thr Phe Thr Asp Gly Arg Tyr Cys 385 390 395 400 Gly Ala Ile Leu Asp Arg Asn Gly Leu Arg Pro Cys Arg Tyr Tyr Ile 405 410 415 Thr Ser Asp Asp Arg Val Ile Cys Ala Ser Glu Val Gly Val Ile Pro 420 425 430 Ile Glu Asn Ser Leu Val Val Gln Lys Gly Lys Leu Lys Pro Gly Asp 435 440 445 Leu Phe Leu Val Asp Thr Gln Leu Gly Glu Met Val Asp Thr Lys Lys 450 455 460 Leu Lys Ser Gln Ile Ser Lys Arg Gln Asp Phe Lys Ser Trp Leu Ser 465 470 475 480 Lys Val Ile Lys Leu Asp Asp Leu Leu Ser Lys Thr Ala Asn Leu Val 485 490 495 Pro Lys Glu Phe Ile Ser Gln Asp Ser Leu Ser Leu Lys Val Gln Ser 500 505 510 Asp Pro Arg Leu Leu Ala Asn Gly Tyr Thr Phe Glu Gln Val Thr Phe 515 520 525 Leu Leu Thr Pro Met Ala Leu Thr Gly Lys Glu Ala Leu Gly Ser Met 530 535 540 Gly Asn Asp Ala Pro Leu Ala Cys Leu Asn Glu Asn Pro Val Leu Leu 545 550 555 560 Tyr Asp Tyr Phe Arg Gln Leu Phe Ala Gln Val Thr Asn Pro Pro Ile 565 570 575 Asp Pro Ile Arg Glu Ala Asn Val Met Ser Leu Glu Cys Tyr Val Gly 580 585 590 Pro Gln Gly Asn Leu Leu Glu Met His Ser Ser Gln Cys Asp Arg Leu 595 600 605 Leu Leu Lys Ser Pro Ile Leu His Trp Asn Glu Phe Gln Ala Leu Lys 610 615 620 Asn Ile Glu Ala Ala Tyr Pro Ser Trp Ser Val Ala Glu Ile Asp Ile 625 630 635 640 Thr Phe Asp Lys Ser Glu Gly Leu Leu Gly Tyr Thr Asp Thr Ile Asp 645 650 655 Lys Ile Thr Lys Leu Ala Ser Glu Ala Ile Asp Asp Gly Lys Lys Ile 660 665 670 Leu Ile Ile Thr Asp Arg Lys Met Gly Ala Asn Arg Val Ser Ile Ser 675 680 685 Ser Leu Ile Ala Ile Ser Cys Ile His His His Leu Ile Arg Asn Lys 690 695 700 Gln Arg Ser Gln Val Ala Leu Ile Leu Glu Thr Gly Glu Ala Arg Glu 705 710 715 720 Ile His His Phe Cys Val Leu Leu Gly Tyr Gly Cys Asp Gly Val Tyr 725 730 735 Pro Tyr Leu Ala Met Glu Thr Leu Val Arg Met Asn Arg Glu Gly Leu 740 745 750 Leu Arg Asn Val Asn Asn Asp Asn Asp Thr Leu Glu Glu Gly Gln Ile 755 760 765 Leu Glu Asn Tyr Lys His Ala Ile Asp Ala Gly Ile Leu Lys Val Met 770 775 780 Ser Lys Met Gly Ile Ser Thr Leu Ala Ser Tyr Lys Gly Ala Gln Ile 785 790 795 800 Phe Glu Ala Leu Gly Leu Asp Asn Ser Ile Val Asp Leu Cys Phe Thr 805 810 815 Gly Thr Ser Ser Arg Ile Arg Gly Val Thr Phe Glu Tyr Leu Ala Gln 820 825 830 Asp Ala Phe Ser Leu His Glu Arg Gly Tyr Pro Ser Arg Gln Thr Ile 835 840 845 Ser Lys Ser Val Asn Leu Pro Glu Ser Gly Glu Tyr His Phe Arg Asp 850 855 860 Gly Gly Tyr Lys His Val Asn Glu Pro Thr Ala Ile Ala Ser Leu Gln 865 870 875 880 Asp Thr Val Arg Asn Lys Asn Asp Val Ser Trp Gln Leu Tyr Val Lys 885 890 895 Lys Glu Met Glu Ala Ile Arg Asp Cys Thr Leu Arg Gly Leu Leu Glu 900 905 910 Leu Asp Phe Glu Asn Ser Val Ser Ile Pro Leu Glu Gln Val Glu Pro 915 920 925 Trp Thr Glu Ile Ala Arg Arg Phe Ala Ser Gly Ala Met Ser Tyr Gly 930 935 940 Ser Ile Ser Met Glu Ala His Ser Thr Leu Ala Ile Ala Met Asn Arg 945 950 955 960 Leu Gly Ala Lys Ser Asn Cys Gly Glu Gly Gly Glu Asp Ala Glu Arg 965 970 975 Ser Ala Val Gln Glu Asn Gly Asp Thr Met Arg Ser Ala Ile Lys Gln 980 985 990 Val Ala Ser Ala Arg Phe Gly Val Thr Ser Tyr Tyr Leu Ser Asp Ala 995 1000 1005 Asp Glu Ile Gln Ile Lys Ile Ala Gln Gly Ala Lys Pro Gly Glu Gly 1010 1015 1020 Gly Glu Leu Pro Ala His Lys Val Ser Lys Asp Ile Ala Lys Thr Arg 1025 1030 1035 1040 His Ser Thr Pro Asn Val Gly Leu Ile Ser Pro Pro Pro His His Asp 1045 1050 1055 Ile Tyr Ser Ile Glu Asp Leu Lys Gln Leu Ile Tyr Asp Leu Lys Cys 1060 1065 1070 Ala Asn Pro Arg Ala Gly Ile Ser Val Lys Leu Val Ser Glu Val Gly 1075 1080 1085 Val Gly Ile Val Ala Ser Gly Val Ala Lys Ala Lys Ala Asp His Ile 1090 1095 1100 Leu Val Ser Gly His Asp Gly Gly Thr Gly Ala Ala Arg Trp Thr Ser 1105 1110 1115 1120 Val Lys Tyr Ala Gly Leu Pro Trp Glu Leu Gly Leu Ala Glu Thr His 1125 1130 1135 Gln Thr Leu Val Leu Asn Asp Leu Arg Arg Asn Val Val Val Gln Thr 1140 1145 1150 Asp Gly Gln Leu Arg Thr Gly Phe Asp Ile Ala Val Ala Val Leu Leu 1155 1160 1165 Gly Ala Glu Ser Phe Thr Leu Ala Thr Val Pro Leu Ile Ala Met Gly 1170 1175 1180 Cys Val Met Leu Arg Arg Cys His Leu Asn Ser Cys Ala Val Gly Ile 1185 1190 1195 1200 Ala Thr Gln Asp Pro Tyr Leu Arg Ser Lys Phe Lys Gly Gln Pro Glu 1205 1210 1215 His Val Ile Asn Phe Phe Tyr Tyr Leu Ile Gln Asp Leu Arg Gln Ile 1220 1225 1230 Met Ala Lys Leu Gly Phe Arg Thr Ile Asp Glu Met Val Gly His Ser 1235 1240 1245 Glu Lys Leu Lys Lys Arg Asp Asp Val Asn Ala Lys Ala Ile Asn Ile 1250 1255 1260 Asp Leu Ser Pro Ile Leu Thr Pro Ala His Val Ile Arg Pro Gly Val 1265 1270 1275 1280 Pro Thr Lys Phe Thr Lys Lys Gln Asp His Lys Leu His Thr Arg Leu 1285 1290 1295 Asp Asn Lys Leu Ile Asp Glu Ala Glu Val Thr Leu Asp Arg Gly Leu 1300 1305 1310 Pro Val Asn Ile Asp Ala Ser Ile Ile Asn Thr Asp Arg Ala Leu Gly 1315 1320 1325 Ser Thr Leu Ser Tyr Arg Val Ser Lys Lys Phe Gly Glu Asp Gly Leu 1330 1335 1340 Pro Lys Asp Thr Val Val Val Asn Ile Glu Gly Ser Ala Gly Gln Ser 1345 1350 1355 1360 Phe Gly Ala Phe Leu Ala Ser Gly Ile Thr Phe Ile Leu Asn Gly Asp 1365 1370 1375 Ala Asn Asp Tyr Val Gly Lys Gly Leu Ser Gly Gly Ile Ile Val Ile 1380 1385 1390 Lys Pro Pro Lys Asp Ser Lys Phe Lys Ser Asp Glu Asn Val Ile Val 1395 1400 1405 Gly Asn Thr Cys Phe Tyr Gly Ala Thr Ser Gly Thr Ala Phe Ile Ser 1410 1415 1420 Gly Ser Ala Gly Glu Arg Phe Gly Val Arg Asn Ser Gly Ala Thr Ile 1425 1430 1435 1440 Val Val Glu Arg Ile Lys Gly Asn Asn Ala Phe Glu Tyr Met Thr Gly 1445 1450 1455 Gly Arg Ala Ile Val Leu Ser Gln Met Glu Ser Leu Asn Ala Phe Ser 1460 1465 1470 Gly Ala Thr Gly Gly Ile Ala Tyr Cys Leu Thr Ser Asp Tyr Asp Asp 1475 1480 1485 Phe Val Gly Lys Ile Asn Lys Asp Thr Val Glu Leu Glu Ser Leu Cys 1490 1495 1500 Asp Pro Val Glu Ile Ala Phe Val Lys Asn Leu Ile Gln Glu His Trp 1505 1510 1515 1520 Asn Tyr Thr Gln Ser Asp Leu Ala Ala Arg Ile Leu Gly Asn Phe Asn 1525 1530 1535 His Tyr Leu Lys Asp Phe Val Lys Val Ile Pro Thr Asp Tyr Lys Lys 1540 1545 1550 Val Leu Leu Lys Glu Lys Ala Glu Ala Ala Lys Ala Lys Ala Lys Ala 1555 1560 1565 Thr Ser Glu Tyr Leu Lys Lys Phe Arg Ser Asn Gln Glu Val Asp Asp 1570 1575 1580 Glu Val Asn Thr Leu Leu Ile Ala Asn Gln Lys Ala Lys Glu Gln Glu 1585 1590 1595 1600 Lys Lys Lys Ser Ile Thr Ile Ser Asn Lys Ala Thr Leu Lys Glu Pro 1605 1610 1615 Lys Val Val Asp Leu Glu Asp Ala Val Pro Asp Ser Lys Gln Leu Glu 1620 1625 1630 Lys Asn Ser Glu Arg Ile Glu Lys Thr Arg Gly Phe Met Ile His Lys 1635 1640 1645 Arg Arg His Glu Thr His Arg Asp Pro Arg Thr Arg Val Asn Asp Trp 1650 1655 1660 Lys Glu Phe Thr Asn Pro Ile Thr Lys Lys Asp Ala Lys Tyr Gln Thr 1665 1670 1675 1680 Ala Arg Cys Met Asp Cys Gly Thr Pro Phe Cys Leu Ser Asp Thr Gly 1685 1690 1695 Cys Pro Leu Ser Asn Ile Ile Pro Lys Phe Asn Glu Leu Leu Phe Lys 1700 1705 1710 Asn Gln Trp Lys Leu Ala Leu Asp Lys Leu Leu Glu Thr Asn Asn Phe 1715 1720 1725 Pro Glu Phe Thr Gly Arg Val Cys Pro Ala Pro Cys Glu Gly Ala Cys 1730 1735 1740 Thr Leu Gly Ile Ile Glu Asp Pro Val Gly Ile Lys Ser Val Glu Arg 1745 1750 1755 1760 Ile Ile Ile Asp Asn Ala Phe Lys Glu Gly Trp Ile Lys Pro Cys Pro 1765 1770 1775 Pro Ser Thr Arg Thr Gly Phe Thr Val Gly Val Ile Gly Ser Gly Pro 1780 1785 1790 Ala Gly Leu Ala Cys Ala Asp Met Leu Asn Arg Ala Gly His Thr Val 1795 1800 1805 Thr Val Tyr Glu Arg Ser Asp Arg Cys Gly Gly Leu Leu Met Tyr Gly 1810 1815 1820 Ile Pro Asn Met Lys Leu Asp Lys Ala Ile Val Gln Arg Arg Ile Asp 1825 1830 1835 1840 Leu Leu Ser Ala Glu Gly Ile Asp Phe Val Thr Asn Thr Glu Ile Gly 1845 1850 1855 Lys Thr Ile Ser Met Asp Glu Leu Lys Asn Lys His Asn Ala Val Val 1860 1865 1870 Tyr Ala Ile Gly Ser Thr Ile Pro Arg Asp Leu Pro Ile Lys Gly Arg 1875 1880 1885 Glu Leu Lys Asn Ile Asp Phe Ala Met Gln Leu Leu Glu Ser Asn Thr 1890 1895 1900 Lys Ala Leu Leu Asn Lys Asp Leu Glu Ile Ile Arg Glu Lys Ile Gln 1905 1910 1915 1920 Gly Lys Lys Val Ile Val Val Gly Gly Gly Asp Thr Gly Asn Asp Cys 1925 1930 1935 Leu Gly Thr Ser Val Arg His Gly Ala Ala Ser Val Leu Asn Phe Glu 1940 1945 1950 Leu Leu Pro Glu Pro Pro Val Glu Arg Ala Lys Asp Asn Pro Trp Pro 1955 1960 1965 Gln Trp Pro Arg Val Met Arg Val Asp Tyr Gly His Ala Glu Val Lys 1970 1975 1980 Glu His Tyr Gly Arg Asp Pro Arg Glu Tyr Cys Ile Leu Ser Lys Glu 1985 1990 1995 2000 Phe Ile Gly Asn Asp Glu Gly Glu Val Thr Ala Ile Arg Thr Val Arg 2005 2010 2015 Val Glu Trp Lys Lys Ser Gln Ser Gly Val Trp Gln Met Val Glu Ile 2020 2025 2030 Pro Asn Ser Glu Glu Ile Phe Glu Ala Asp Ile Ile Leu Leu Ser Met 2035 2040 2045 Gly Phe Val Gly Pro Glu Leu Ile Asn Gly Asn Asp Asn Glu Val Lys 2050 2055 2060 Lys Thr Arg Arg Gly Thr Ile Ala Thr Leu Asp Asp Ser Ser Tyr Ser 2065 2070 2075 2080 Ile Asp Gly Gly Lys Thr Phe Ala Cys Gly Asp Cys Arg Arg Gly Gln 2085 2090 2095 Ser Leu Ile Val Trp Ala Ile Gln Glu Gly Arg Lys Cys Ala Ala Ser 2100 2105 2110 Val Asp Lys Phe Leu Met Asp Gly Thr Thr Tyr Leu Pro Ser Asn Gly 2115 2120 2125 Gly Ile Val Gln Arg Asp Tyr Lys Leu Leu Lys Glu Leu Ala Ser Gln 2130 2135 2140 Val 2145 <210> 100 <211> 300 <212> DNA <213> Saccharomyces cerevisiae <220> <223> nucleic acid sequence of terminator tTDH3 <220> <223> nucleic acid sequence of terminator tTDH3 <400> 100 gtgaatttac tttaaatctt gcatttaaat aaattttctt tttatagctt tatgacttag 60 tttcaattta tatactattt taatgacatt ttcgattcat tgattgaaag ctttgtgttt 120 tttcttgatg cgctattgca ttgttcttgt ctttttcgcc acatgtaata tctgtagtag 180 atacctgata cattgtggat gctgagtgaa attttagtta ataatggagg cgctcttaat 240 aattttgggg atattggctt ttttttttaa agtttacaaa tgaatttttt ccgccaggat 300 <210> 101 <211> 1788 <212> DNA <213> Artificial Sequence <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CviKI <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CviKI <400> 101 atgcttttca gttgtttgtc cttcgtgatc tgccatctta gttttatttt gcgttattac 60 aagaggcttc taaaagtgac gatgggaaag aacattataa taatggtgag ttggtacaca 120 ataataactt ccaacatcat cgccgtcggt ggggcgtctc tgattttggc cccagccatc 180 actggataca tcttgcactg gaacatcgcc ttgtcaacca tctggggagt ttccgcttat 240 gggatattcg tgttcggatt cttcctggct caggttttat ttagtgagct aaatcgtaaa 300 agactaagaa agtggatttc tctaagacca aagggttgga acgacgtacg tctggctgtc 360 atcatagctg gttacaggga agacccgtac atgtttcaga aatgccttga gtcagtaagg 420 gactctgact atggtaatgt cgctcgtctt atctgtgtaa ttgacggaga tgaggacgac 480 gacatgaaga tggcagccgt ttataaggcg atatataatg acaatatcaa gaagccagag 540 tttgtgttat gtgagagcga tgataaggaa ggcgagagaa ttgactcaga ttttagcagg 600 gacatctgcg tcttacaacc acaccgtggc aaaagggagt gcttgtatac cggctttcag 660 ctggccaaaa tggacccgtc ggtaaacgcc gttgttctta ttgactctga cacggtcttg 720 gaaaaggacg caatcttgga agtcgtgtat ccattggcct gtgacccgga aattcaagct 780 gtcgcaggag agtgcaaaat atggaacaca gatacacttt tgtcgttgtt ggtggcttgg 840 agatattatt cagccttttg cgtggagaga tccgcccaat cattctttag gacagtccag 900 tgtgtaggtg gacccttggg cgcctacaag atagatatca tcaaagagat caaagatccc 960 tggatctccc aacgtttctt aggtcagaag tgcacctacg gtgacgaccg taggttaaca 1020 aatgaaatct taatgagggg taagaaggtc gtcttcactc ctttcgcagt tggctggagt 1080 gactcaccaa ccaacgtgtt caggtacatc gtacaacaga ctaggtggag taaatcatgg 1140 tgccgtgaga tatggtacac tttgtttgct gcgtggaagc acggactaag cggaatttgg 1200 ttggcatttg agtgccttta tcagattacg tacttcttcc tagtcatata tttattttcg 1260 agactagccg ttgaagctga cccaagagca cagaccgcaa cagtaatcgt gtctaccacg 1320 gtagccctga ttaagtgtgg ttacttctct tttcgtgcga aagatattcg tgctttctac 1380 ttcgtcctgt acacatttgt ctacttcttc tgtatgatcc cagcaagggt gacagccatg 1440 atgacacttt gggatatcgg ctggggcacg cgtggcggaa acgagaaacc aagtgtcggc 1500 actagggtcg ctctgtgggc taaacagtat ttgatagcat acatgtggtg ggcagcagtt 1560 gttggcgctg gggtatactc cattgttcat aattggatgt ttgactggaa ctcactatcc 1620 tataggttcg cacttgtagg tatatgctct tacatcgtat tcatcacaat cgttctagtc 1680 atctatttca caggaaagat taccacatgg aatttcacca aattacagaa ggagttaatc 1740 aaggataggg tcttatacga tgcttccact aacgcccaga ctgtataa 1788 <210> 102 <211> 1803 <212> DNA <213> Artificial Sequence <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus IL-5-2s1 <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus IL-5-2s1 <400> 102 atggctgctt cattctatgt tatcttcttg ttcgtaatat gccacctgtc cttcgtctta 60 tatcgtcaag ttctatacat aattttgggt agatactgga gtgttaacaa gctgacaatg 120 atctcttggt ataccattat atcatctaat ctaatcgcaa tcggtggggc ttcccttatt 180 ttggcaccgg ccataaccgg ctacattttg cattggaaca tagctctaag cacaatttgg 240 ggtgtgtcag cctatggcat tttcgtgttc gggttctttc ttgctcaggt tttgttcagt 300 gagcttaaca ggaagaggtt gagaaaatgg atctctttga gaccagataa ctggaacgcg 360 gtgagagttg ccgtgataat cgctggttac cgtgaagacc catacatgtt tcaaaagtgt 420 ttggaatcgg tgagagactc cgattatggt aacattgcga ggcttatatg cgttatagat 480 ggggacgaag atgacgacat gaagatggca gatgtataca aggctattta caatgacaat 540 ataaagaagc cggaaattat cctatgtgag tccgacgaca aagagggaga aaggatagac 600 agtgatttta gccgtgatat ttgcgtgctt cagcctcaca gaggtaagcg tgaatgttta 660 tatactggct ttcagttagc taaaatggac ccctctgtac acgcggttgt gcttattgac 720 tcggatacag ttttggagaa ggatgccatc ctggaagtgg tctacccact tgcttgcgac 780 catgagattc aagcagtcgc aggtgaatgc aagatatgga atacggacac cttattatcc 840 atattagtcg cctggcgtta ctactcagct ttctgcgttg agcgtagcgc tcagtccttc 900 ttccgtactg tgcaatgcgt cggcggtccg ttaggggcat acaaaataga cataattaaa 960 gagataaagg agccgtggat ttcacaaaga tttctgggtc agaagtgtac gtacggagat 1020 gacagaagat taactaacga agtgttgatg cgtggaaaga aagtagtctt cacacctttt 1080 gcggtaggat ggagcgactc gcctactaat gtcttccgtt atattgtgca gcagacgagg 1140 tggagtaaga gctggtgcag ggagatttgg tatacactgt tcgcggcgtg gaagcatggg 1200 ttatcaggca tttggttggc atttgagtgc ttgtatcaga tcacatactt ctttctagtg 1260 atatatctgt tcagtagact agcagtcgag gctgacccga gagcgcaaac tgcaacggtt 1320 atagtcagca caatggtttc actaatcaag tgtggatatt tctcattccg tgctaaggac 1380 attagagcgt tttatttcgt actatatact tttgtgtact tcttttgcat gatcccagca 1440 agagtcactg ctatgatgac cttgtgggat attgggtggg gtactagggg tggaaacgtg 1500 aaaccatcga tcggaactag gatctctttg tgggctaagc agtacttaat cgcgtacatg 1560 tggtgggcag ctgtgatcgc cgcaggagtc tatagtataa tacataattg gatgtttgat 1620 tggaactcct tgtcctatcg tttcgcgtta ataggcatct gctcgtatat cgtattcatt 1680 gccattgtta tagtgatata cttcacaggt aagatcacca cttggaattt taccaagcta 1740 cagaaagagt taattgaaga tagagtcttg catgatgccg atgtggacat acagaacgta 1800 taa 1803 <210> 103 <211> 1686 <212> DNA <213> Artificial Sequence <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CZ-2 <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CZ-2 <400> 103 atgacatctt ggagaacgat tgtgtcagct aaccttttcg cagttggtgg ggctcttcta 60 atgttagccc cagctatcgt gggctatgta tttcagtgga atataggcgt gtccgcagtc 120 tggggcatct ccgtatacgg cgtctttgtg ttgggatttt atatcgctca aatcgtcttc 180 tctgagttca acaggatgag attatccgat tggataagtc ttagaccaga caactggaac 240 gccacaaggg ttgcggtcat tatagctgga tacagagaag atccttttat gttcaagaag 300 tgcttagaga gtgtgaggga ttcggaatac ggcaacgtag caaggcttat atgcgtaatt 360 gacggagacg aagaagagga cctaaagatg gctgagattt acaagcaggt ttacaatgac 420 aacgtgaaga aaccaggtgt agtgttatgc gaatcggaga acaagaatgg atcgactata 480 gattcggacg tatctaagaa tatatgcata ctgcaacccc accgtgggaa acgtgagagc 540 ctatataccg gtttccaatt ggcaagcatg gacccttccg tacatgcggt tgttcttata 600 gattccgaca ctgtattaga gaagaatgct attcttgagg tcgtataccc tctatcttgt 660 gaccccaaca ttaaggccgt cgccggggag tgcaagatat ggaatacaga caccatcttg 720 tccatgcttg tctcttggag atacttctct gccttcaatg tcgagcgtgg agcccagtca 780 ttatggaaga ctgtgcaatg cgtaggcggg ccacttggcg catacactat cgacatcata 840 aatgaaatta aagacccgtg gattacccag acattcctgg gcaacaagtg cacttatggc 900 gacgatagac gtcttaccaa tgaggtttta atgaggggca agaagattgt atacactccg 960 tttgccgtcg gttggagcga cagtccgacg aatgtcatga ggtacattgt gcagcaaact 1020 agatggagta agtcatggtg cagagagata tggtacacgt taggcagtgc ctggaaacat 1080 ggattctccg gcatatacct tgcgtttgag tgtatgtacc agataatgta cttcttctta 1140 gtcatgtacc tgttttcgta tatcgcaata aaggcagaca tccgtgctca gactgcaact 1200 gtgttggttt ctactctagt caccataata aagagcagtt acctagcgct gagggccaag 1260 aacctgaagg ctttctattt cgttctgtat acctatgtct atttcttttg catgatacct 1320 gctaggatta ccgctatgtt tacaatgttt gatatagctt ggggtacacg tggtggtaat 1380 gcgaaaatga caatcggggc tagagtttgg ctgtgggcga agcagttctt aataacttat 1440 atgtggtggg caggagtact agcggcggga gtttacagta tagttgacaa ttggtacttc 1500 gattgggctg atatacagta caggtttgca ttggtaggta tttgctcgta tttggtcttt 1560 gtcagtattg tactggtaat ctacttgatc ggtaagataa caacgtggaa ctacacaccg 1620 ctacagaagg aacttattga ggagagatac ttgcataacg cctctgagaa tgccccggaa 1680 gtttaa 1686 <210> 104 <211> 1677 <212> DNA <213> Artificial Sequence <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CVG-1 <220> <223> reencoded nucleic acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CVG-1 <400> 104 atgacttctt ggcgtacaat tgtttctgct aacttgttcg cagttggtgg cgccttgttg 60 atgttagctc ccgcgatagc cggttatgta tttaaatgga acataggcgt ttctgccgtt 120 tggggtattt cagtgtacgg ggtcttcgtg cttggtttct atattgcaca ggtagtcttc 180 agcgagttta ataggatgca gctgtcagac tggatctcat tgagacccga caactggaac 240 gccaccagag tagcggtaat tattgctggt tacagggaag acccctttat gttcaagaag 300 tgcctagaat ctgtgagaga ctcagaatac gggaacatag cgaggctaat ctgtgttatc 360 gacggcgatg aagaggaaga ccttaagatg gctgagatat acaagcaagt atacaacgac 420 aacgtcaaga cccccggtgt tgtgctatgc gagaatgaaa ataagaatgg tagcaccatt 480 gacccagatt ttagtaagaa catctgcatt ctgcaaccac acaggggcaa acgtgaatct 540 ttgtacaccg gattccagat ggctggcatg gacccatcag tgcacgcagt tgtacttata 600 gacagtgata ctgttcttga aaagaacgct atcctagagg ttgtgtaccc tctatcttgt 660 gatccaaata tcaaagctgt cgcgggagag tgtaaaatct ggaacacaga taccattttg 720 tctatgctag tttcatggcg ttatttctcg gctttcaacg tggagagagg cgcacagtcc 780 ctttggaaaa cagtccagtg tgtcggcggg cctttgggag catacactat agacatcata 840 aacgagataa aagacccctg gattactcag acattcttag gaaacaaatg tacgtatggt 900 gacgacagaa ggttaaccaa tgaagtgctt atgaggggaa agaagattgt ctatactccc 960 tttgctgtcg gttggtccga ttcgcccaca aacgttatga ggtacatcgt ccagcagaca 1020 aggtggtcta agagctggtg ccgtgagatt tggtacacct tgggatcagc atggaaacat 1080 ggtttctccg gaatctactt agcgttcgag tgcatgtacc agattatgta tttctttatt 1140 gtgatgtatt tgttcagtta cattgccatt aaagccaaca tcagggcaca agcagccacg 1200 gtattggtca gtacgcttgt cgcagtaatc aaatctagtt atttggcatt aagagcgaag 1260 aacctaaaag ctttgtattt cgtcctgtat acctacgtct acttcttctg catgattcca 1320 gctagaataa cagcgatgtt cacgatgttc gatattgcat ggggtactag aggcggtaac 1380 gcaaagatga cgatcggtgc tagagtctgg ttgtgggcta aacaattctt aattacatac 1440 atgtggtggg tgggagtttt ggccgctggg gtctacagta tagtcgataa ctggtacttc 1500 gactgggcgg acatccagta cagattcgcg ctagtgggga tttgcagtta cttaggtttc 1560 gtttccataa tgctggtatt ctatctgatt ggtaaaataa ctacgtggaa ttacactcct 1620 ctacaaaagg agctgattaa agagaggtta cacgccgcta atgcaacaga ggtctaa 1677 <210> 105 <211> 595 <212> PRT <213> Artificial Sequence <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CviKI <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CviKI <400> 105 Met Leu Phe Ser Cys Leu Ser Phe Val Ile Cys His Leu Ser Phe Ile 1 5 10 15 Leu Arg Tyr Tyr Lys Arg Leu Leu Lys Val Thr Met Gly Lys Asn Ile 20 25 30 Ile Ile Met Val Ser Trp Tyr Thr Ile Ile Thr Ser Asn Ile Ile Ala 35 40 45 Val Gly Gly Ala Ser Leu Ile Leu Ala Pro Ala Ile Thr Gly Tyr Ile 50 55 60 Leu His Trp Asn Ile Ala Leu Ser Thr Ile Trp Gly Val Ser Ala Tyr 65 70 75 80 Gly Ile Phe Val Phe Gly Phe Phe Leu Ala Gln Val Leu Phe Ser Glu 85 90 95 Leu Asn Arg Lys Arg Leu Arg Lys Trp Ile Ser Leu Arg Pro Lys Gly 100 105 110 Trp Asn Asp Val Arg Leu Ala Val Ile Ile Ala Gly Tyr Arg Glu Asp 115 120 125 Pro Tyr Met Phe Gln Lys Cys Leu Glu Ser Val Arg Asp Ser Asp Tyr 130 135 140 Gly Asn Val Ala Arg Leu Ile Cys Val Ile Asp Gly Asp Glu Asp Asp 145 150 155 160 Asp Met Lys Met Ala Ala Val Tyr Lys Ala Ile Tyr Asn Asp Asn Ile 165 170 175 Lys Lys Pro Glu Phe Val Leu Cys Glu Ser Asp Asp Lys Glu Gly Glu 180 185 190 Arg Ile Asp Ser Asp Phe Ser Arg Asp Ile Cys Val Leu Gln Pro His 195 200 205 Arg Gly Lys Arg Glu Cys Leu Tyr Thr Gly Phe Gln Leu Ala Lys Met 210 215 220 Asp Pro Ser Val Asn Ala Val Val Leu Ile Asp Ser Asp Thr Val Leu 225 230 235 240 Glu Lys Asp Ala Ile Leu Glu Val Val Tyr Pro Leu Ala Cys Asp Pro 245 250 255 Glu Ile Gln Ala Val Ala Gly Glu Cys Lys Ile Trp Asn Thr Asp Thr 260 265 270 Leu Leu Ser Leu Leu Val Ala Trp Arg Tyr Tyr Ser Ala Phe Cys Val 275 280 285 Glu Arg Ser Ala Gln Ser Phe Phe Arg Thr Val Gln Cys Val Gly Gly 290 295 300 Pro Leu Gly Ala Tyr Lys Ile Asp Ile Ile Lys Glu Ile Lys Asp Pro 305 310 315 320 Trp Ile Ser Gln Arg Phe Leu Gly Gln Lys Cys Thr Tyr Gly Asp Asp 325 330 335 Arg Arg Leu Thr Asn Glu Ile Leu Met Arg Gly Lys Lys Val Val Phe 340 345 350 Thr Pro Phe Ala Val Gly Trp Ser Asp Ser Pro Thr Asn Val Phe Arg 355 360 365 Tyr Ile Val Gln Gln Thr Arg Trp Ser Lys Ser Trp Cys Arg Glu Ile 370 375 380 Trp Tyr Thr Leu Phe Ala Ala Trp Lys His Gly Leu Ser Gly Ile Trp 385 390 395 400 Leu Ala Phe Glu Cys Leu Tyr Gln Ile Thr Tyr Phe Phe Leu Val Ile 405 410 415 Tyr Leu Phe Ser Arg Leu Ala Val Glu Ala Asp Pro Arg Ala Gln Thr 420 425 430 Ala Thr Val Ile Val Ser Thr Thr Val Ala Leu Ile Lys Cys Gly Tyr 435 440 445 Phe Ser Phe Arg Ala Lys Asp Ile Arg Ala Phe Tyr Phe Val Leu Tyr 450 455 460 Thr Phe Val Tyr Phe Phe Cys Met Ile Pro Ala Arg Val Thr Ala Met 465 470 475 480 Met Thr Leu Trp Asp Ile Gly Trp Gly Thr Arg Gly Gly Asn Glu Lys 485 490 495 Pro Ser Val Gly Thr Arg Val Ala Leu Trp Ala Lys Gln Tyr Leu Ile 500 505 510 Ala Tyr Met Trp Trp Ala Ala Val Val Gly Ala Gly Val Tyr Ser Ile 515 520 525 Val His Asn Trp Met Phe Asp Trp Asn Ser Leu Ser Tyr Arg Phe Ala 530 535 540 Leu Val Gly Ile Cys Ser Tyr Ile Val Phe Ile Thr Ile Val Leu Val 545 550 555 560 Ile Tyr Phe Thr Gly Lys Ile Thr Thr Trp Asn Phe Thr Lys Leu Gln 565 570 575 Lys Glu Leu Ile Lys Asp Arg Val Leu Tyr Asp Ala Ser Thr Asn Ala 580 585 590 Gln Thr Val 595 <210> 106 <211> 600 <212> PRT <213> Artificial Sequence <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus IL-5-2s1 <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus IL-5-2s1 <400> 106 Met Ala Ala Ser Phe Tyr Val Ile Phe Leu Phe Val Ile Cys His Leu 1 5 10 15 Ser Phe Val Leu Tyr Arg Gln Val Leu Tyr Ile Ile Leu Gly Arg Tyr 20 25 30 Trp Ser Val Asn Lys Leu Thr Met Ile Ser Trp Tyr Thr Ile Ile Ser 35 40 45 Ser Asn Leu Ile Ala Ile Gly Gly Ala Ser Leu Ile Leu Ala Pro Ala 50 55 60 Ile Thr Gly Tyr Ile Leu His Trp Asn Ile Ala Leu Ser Thr Ile Trp 65 70 75 80 Gly Val Ser Ala Tyr Gly Ile Phe Val Phe Gly Phe Phe Leu Ala Gln 85 90 95 Val Leu Phe Ser Glu Leu Asn Arg Lys Arg Leu Arg Lys Trp Ile Ser 100 105 110 Leu Arg Pro Asp Asn Trp Asn Ala Val Arg Val Ala Val Ile Ile Ala 115 120 125 Gly Tyr Arg Glu Asp Pro Tyr Met Phe Gln Lys Cys Leu Glu Ser Val 130 135 140 Arg Asp Ser Asp Tyr Gly Asn Ile Ala Arg Leu Ile Cys Val Ile Asp 145 150 155 160 Gly Asp Glu Asp Asp Asp Met Lys Met Ala Asp Val Tyr Lys Ala Ile 165 170 175 Tyr Asn Asp Asn Ile Lys Lys Pro Glu Ile Ile Leu Cys Glu Ser Asp 180 185 190 Asp Lys Glu Gly Glu Arg Ile Asp Ser Asp Phe Ser Arg Asp Ile Cys 195 200 205 Val Leu Gln Pro His Arg Gly Lys Arg Glu Cys Leu Tyr Thr Gly Phe 210 215 220 Gln Leu Ala Lys Met Asp Pro Ser Val His Ala Val Val Leu Ile Asp 225 230 235 240 Ser Asp Thr Val Leu Glu Lys Asp Ala Ile Leu Glu Val Val Tyr Pro 245 250 255 Leu Ala Cys Asp His Glu Ile Gln Ala Val Ala Gly Glu Cys Lys Ile 260 265 270 Trp Asn Thr Asp Thr Leu Leu Ser Ile Leu Val Ala Trp Arg Tyr Tyr 275 280 285 Ser Ala Phe Cys Val Glu Arg Ser Ala Gln Ser Phe Phe Arg Thr Val 290 295 300 Gln Cys Val Gly Gly Pro Leu Gly Ala Tyr Lys Ile Asp Ile Ile Lys 305 310 315 320 Glu Ile Lys Glu Pro Trp Ile Ser Gln Arg Phe Leu Gly Gln Lys Cys 325 330 335 Thr Tyr Gly Asp Asp Arg Arg Leu Thr Asn Glu Val Leu Met Arg Gly 340 345 350 Lys Lys Val Val Phe Thr Pro Phe Ala Val Gly Trp Ser Asp Ser Pro 355 360 365 Thr Asn Val Phe Arg Tyr Ile Val Gln Gln Thr Arg Trp Ser Lys Ser 370 375 380 Trp Cys Arg Glu Ile Trp Tyr Thr Leu Phe Ala Ala Trp Lys His Gly 385 390 395 400 Leu Ser Gly Ile Trp Leu Ala Phe Glu Cys Leu Tyr Gln Ile Thr Tyr 405 410 415 Phe Phe Leu Val Ile Tyr Leu Phe Ser Arg Leu Ala Val Glu Ala Asp 420 425 430 Pro Arg Ala Gln Thr Ala Thr Val Ile Val Ser Thr Met Val Ser Leu 435 440 445 Ile Lys Cys Gly Tyr Phe Ser Phe Arg Ala Lys Asp Ile Arg Ala Phe 450 455 460 Tyr Phe Val Leu Tyr Thr Phe Val Tyr Phe Phe Cys Met Ile Pro Ala 465 470 475 480 Arg Val Thr Ala Met Met Thr Leu Trp Asp Ile Gly Trp Gly Thr Arg 485 490 495 Gly Gly Asn Val Lys Pro Ser Ile Gly Thr Arg Ile Ser Leu Trp Ala 500 505 510 Lys Gln Tyr Leu Ile Ala Tyr Met Trp Trp Ala Ala Val Ile Ala Ala 515 520 525 Gly Val Tyr Ser Ile Ile His Asn Trp Met Phe Asp Trp Asn Ser Leu 530 535 540 Ser Tyr Arg Phe Ala Leu Ile Gly Ile Cys Ser Tyr Ile Val Phe Ile 545 550 555 560 Ala Ile Val Ile Val Ile Tyr Phe Thr Gly Lys Ile Thr Thr Trp Asn 565 570 575 Phe Thr Lys Leu Gln Lys Glu Leu Ile Glu Asp Arg Val Leu His Asp 580 585 590 Ala Asp Val Asp Ile Gln Asn Val 595 600 <210> 107 <211> 561 <212> PRT <213> Artificial Sequence <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CZ-2 <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CZ-2 <400> 107 Met Thr Ser Trp Arg Thr Ile Val Ser Ala Asn Leu Phe Ala Val Gly 1 5 10 15 Gly Ala Leu Leu Met Leu Ala Pro Ala Ile Val Gly Tyr Val Phe Gln 20 25 30 Trp Asn Ile Gly Val Ser Ala Val Trp Gly Ile Ser Val Tyr Gly Val 35 40 45 Phe Val Leu Gly Phe Tyr Ile Ala Gln Ile Val Phe Ser Glu Phe Asn 50 55 60 Arg Met Arg Leu Ser Asp Trp Ile Ser Leu Arg Pro Asp Asn Trp Asn 65 70 75 80 Ala Thr Arg Val Ala Val Ile Ile Ala Gly Tyr Arg Glu Asp Pro Phe 85 90 95 Met Phe Lys Lys Cys Leu Glu Ser Val Arg Asp Ser Glu Tyr Gly Asn 100 105 110 Val Ala Arg Leu Ile Cys Val Ile Asp Gly Asp Glu Glu Glu Asp Leu 115 120 125 Lys Met Ala Glu Ile Tyr Lys Gln Val Tyr Asn Asp Asn Val Lys Lys 130 135 140 Pro Gly Val Val Leu Cys Glu Ser Glu Asn Lys Asn Gly Ser Thr Ile 145 150 155 160 Asp Ser Asp Val Ser Lys Asn Ile Cys Ile Leu Gln Pro His Arg Gly 165 170 175 Lys Arg Glu Ser Leu Tyr Thr Gly Phe Gln Leu Ala Ser Met Asp Pro 180 185 190 Ser Val His Ala Val Val Leu Ile Asp Ser Asp Thr Val Leu Glu Lys 195 200 205 Asn Ala Ile Leu Glu Val Val Tyr Pro Leu Ser Cys Asp Pro Asn Ile 210 215 220 Lys Ala Val Ala Gly Glu Cys Lys Ile Trp Asn Thr Asp Thr Ile Leu 225 230 235 240 Ser Met Leu Val Ser Trp Arg Tyr Phe Ser Ala Phe Asn Val Glu Arg 245 250 255 Gly Ala Gln Ser Leu Trp Lys Thr Val Gln Cys Val Gly Gly Pro Leu 260 265 270 Gly Ala Tyr Thr Ile Asp Ile Ile Asn Glu Ile Lys Asp Pro Trp Ile 275 280 285 Thr Gln Thr Phe Leu Gly Asn Lys Cys Thr Tyr Gly Asp Asp Arg Arg 290 295 300 Leu Thr Asn Glu Val Leu Met Arg Gly Lys Lys Ile Val Tyr Thr Pro 305 310 315 320 Phe Ala Val Gly Trp Ser Asp Ser Pro Thr Asn Val Met Arg Tyr Ile 325 330 335 Val Gln Gln Thr Arg Trp Ser Lys Ser Trp Cys Arg Glu Ile Trp Tyr 340 345 350 Thr Leu Gly Ser Ala Trp Lys His Gly Phe Ser Gly Ile Tyr Leu Ala 355 360 365 Phe Glu Cys Met Tyr Gln Ile Met Tyr Phe Phe Leu Val Met Tyr Leu 370 375 380 Phe Ser Tyr Ile Ala Ile Lys Ala Asp Ile Arg Ala Gln Thr Ala Thr 385 390 395 400 Val Leu Val Ser Thr Leu Val Thr Ile Ile Lys Ser Ser Tyr Leu Ala 405 410 415 Leu Arg Ala Lys Asn Leu Lys Ala Phe Tyr Phe Val Leu Tyr Thr Tyr 420 425 430 Val Tyr Phe Phe Cys Met Ile Pro Ala Arg Ile Thr Ala Met Phe Thr 435 440 445 Met Phe Asp Ile Ala Trp Gly Thr Arg Gly Gly Asn Ala Lys Met Thr 450 455 460 Ile Gly Ala Arg Val Trp Leu Trp Ala Lys Gln Phe Leu Ile Thr Tyr 465 470 475 480 Met Trp Trp Ala Gly Val Leu Ala Ala Gly Val Tyr Ser Ile Val Asp 485 490 495 Asn Trp Tyr Phe Asp Trp Ala Asp Ile Gln Tyr Arg Phe Ala Leu Val 500 505 510 Gly Ile Cys Ser Tyr Leu Val Phe Val Ser Ile Val Leu Val Ile Tyr 515 520 525 Leu Ile Gly Lys Ile Thr Thr Trp Asn Tyr Thr Pro Leu Gln Lys Glu 530 535 540 Leu Ile Glu Glu Arg Tyr Leu His Asn Ala Ser Glu Asn Ala Pro Glu 545 550 555 560 Val <210> 108 <211> 558 <212> PRT <213> Artificial Sequence <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CVG-1 <220> <223> amino acid sequence of hyaluronan synthase (HASA) originating from Chlorella virus CVG-1 <400> 108 Met Thr Ser Trp Arg Thr Ile Val Ser Ala Asn Leu Phe Ala Val Gly 1 5 10 15 Gly Ala Leu Leu Met Leu Ala Pro Ala Ile Ala Gly Tyr Val Phe Lys 20 25 30 Trp Asn Ile Gly Val Ser Ala Val Trp Gly Ile Ser Val Tyr Gly Val 35 40 45 Phe Val Leu Gly Phe Tyr Ile Ala Gln Val Val Phe Ser Glu Phe Asn 50 55 60 Arg Met Gln Leu Ser Asp Trp Ile Ser Leu Arg Pro Asp Asn Trp Asn 65 70 75 80 Ala Thr Arg Val Ala Val Ile Ile Ala Gly Tyr Arg Glu Asp Pro Phe 85 90 95 Met Phe Lys Lys Cys Leu Glu Ser Val Arg Asp Ser Glu Tyr Gly Asn 100 105 110 Ile Ala Arg Leu Ile Cys Val Ile Asp Gly Asp Glu Glu Glu Asp Leu 115 120 125 Lys Met Ala Glu Ile Tyr Lys Gln Val Tyr Asn Asp Asn Val Lys Thr 130 135 140 Pro Gly Val Val Leu Cys Glu Asn Glu Asn Lys Asn Gly Ser Thr Ile 145 150 155 160 Asp Pro Asp Phe Ser Lys Asn Ile Cys Ile Leu Gln Pro His Arg Gly 165 170 175 Lys Arg Glu Ser Leu Tyr Thr Gly Phe Gln Met Ala Gly Met Asp Pro 180 185 190 Ser Val His Ala Val Val Leu Ile Asp Ser Asp Thr Val Leu Glu Lys 195 200 205 Asn Ala Ile Leu Glu Val Val Tyr Pro Leu Ser Cys Asp Pro Asn Ile 210 215 220 Lys Ala Val Ala Gly Glu Cys Lys Ile Trp Asn Thr Asp Thr Ile Leu 225 230 235 240 Ser Met Leu Val Ser Trp Arg Tyr Phe Ser Ala Phe Asn Val Glu Arg 245 250 255 Gly Ala Gln Ser Leu Trp Lys Thr Val Gln Cys Val Gly Gly Pro Leu 260 265 270 Gly Ala Tyr Thr Ile Asp Ile Ile Asn Glu Ile Lys Asp Pro Trp Ile 275 280 285 Thr Gln Thr Phe Leu Gly Asn Lys Cys Thr Tyr Gly Asp Asp Arg Arg 290 295 300 Leu Thr Asn Glu Val Leu Met Arg Gly Lys Lys Ile Val Tyr Thr Pro 305 310 315 320 Phe Ala Val Gly Trp Ser Asp Ser Pro Thr Asn Val Met Arg Tyr Ile 325 330 335 Val Gln Gln Thr Arg Trp Ser Lys Ser Trp Cys Arg Glu Ile Trp Tyr 340 345 350 Thr Leu Gly Ser Ala Trp Lys His Gly Phe Ser Gly Ile Tyr Leu Ala 355 360 365 Phe Glu Cys Met Tyr Gln Ile Met Tyr Phe Phe Ile Val Met Tyr Leu 370 375 380 Phe Ser Tyr Ile Ala Ile Lys Ala Asn Ile Arg Ala Gln Ala Ala Thr 385 390 395 400 Val Leu Val Ser Thr Leu Val Ala Val Ile Lys Ser Ser Tyr Leu Ala 405 410 415 Leu Arg Ala Lys Asn Leu Lys Ala Leu Tyr Phe Val Leu Tyr Thr Tyr 420 425 430 Val Tyr Phe Phe Cys Met Ile Pro Ala Arg Ile Thr Ala Met Phe Thr 435 440 445 Met Phe Asp Ile Ala Trp Gly Thr Arg Gly Gly Asn Ala Lys Met Thr 450 455 460 Ile Gly Ala Arg Val Trp Leu Trp Ala Lys Gln Phe Leu Ile Thr Tyr 465 470 475 480 Met Trp Trp Val Gly Val Leu Ala Ala Gly Val Tyr Ser Ile Val Asp 485 490 495 Asn Trp Tyr Phe Asp Trp Ala Asp Ile Gln Tyr Arg Phe Ala Leu Val 500 505 510 Gly Ile Cys Ser Tyr Leu Gly Phe Val Ser Ile Met Leu Val Phe Tyr 515 520 525 Leu Ile Gly Lys Ile Thr Thr Trp Asn Tyr Thr Pro Leu Gln Lys Glu 530 535 540 Leu Ile Lys Glu Arg Leu His Ala Ala Asn Ala Thr Glu Val 545 550 555