LISTA DE SECUENCIAS <110> Instituto de Investigaciones Biomédicas August Pi i Sunyer (IDIBAPS) Hospital Clínico de Barcelona Universidad de Barcelona <120> TERAPIA DE CÉLULAS T CON RECEPTOR DE ANTÍGENO QUIMÉRICO ESPECÍFICO DE CD19 <130> 907 178 <160> 13 <170> PatentIn versión 3.5 <210> 1 <211> 11 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 1 Phe Ala Phe Ser Ser Tyr Trp Met Asn Trp Val 1 5 10 <210> 2 <211> 10 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 2 Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr 1 5 10 <210> 3 <211> 9 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 3 Arg Lys Arg Ile Thr Ala Val Ile Thr 1 5 <210> 4 <211> 15 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 4 Arg Ala Ser Glu Ser Val Asp Asn Phe Gly Asn Ser Phe Met His 1 5 10 15 <210> 5 <211> 9 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 5 Ile Tyr Ile Ala Ser Asn Leu Glu Ser 1 5 <210> 6 <211> 10 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 6 His Gln Asn Asn Glu Asp Pro Leu Thr Phe 1 5 10 <210> 7 <211> 113 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 7 Thr Gly Asn Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 1 5 10 15 Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp 20 25 30 Asn Phe Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Ser Gly Gln 35 40 45 Pro Pro Arg Leu Leu Ile Tyr Ile Ala Ser Asn Leu Glu Ser Gly Val 50 55 60 Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr 65 70 75 80 Ile Asp Pro Val Glu Ala Asp Asp Ala Ala Thr Tyr Tyr Cys His Gln 85 90 95 Asn Asn Glu Asp Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 100 105 110 Lys <210> 8 <211> 124 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 8 His Ser Gln Ile Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro 1 5 10 15 Gly Ser Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Phe Ala Phe Ser 20 25 30 Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu 35 40 45 Trp Ile Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Lys Tyr Asn Val 50 55 60 Lys Phe Arg Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr 65 70 75 80 Ala Tyr Ile Gln Leu Thr Ser Leu Thr Ser Glu Asp Ser Gly Val Tyr 85 90 95 Phe Cys Ala Arg Lys Arg Ile Thr Ala Val Ile Thr Thr Val Phe Asp 100 105 110 Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser 115 120 <210> 9 <211> 69 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 9 Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 1 5 10 15 Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 20 25 30 Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 35 40 45 Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 50 55 60 Ile Thr Leu Tyr Cys 65 <210> 10 <211> 42 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 10 Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 1 5 10 15 Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 20 25 30 Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 35 40 <210> 11 <211> 113 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 11 Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 1 5 10 15 Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 20 25 30 Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 35 40 45 Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 50 55 60 Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 65 70 75 80 Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 85 90 95 Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 100 105 110 Arg <210> 12 <211> 481 <212> PRT <213> Secuencia artificial <220> <223> Secuencia artificial <400> 12 Thr Gly Asn Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser 1 5 10 15 Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp 20 25 30 Asn Phe Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Ser Gly Gln 35 40 45 Pro Pro Arg Leu Leu Ile Tyr Ile Ala Ser Asn Leu Glu Ser Gly Val 50 55 60 Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr 65 70 75 80 Ile Asp Pro Val Glu Ala Asp Asp Ala Ala Thr Tyr Tyr Cys His Gln 85 90 95 Asn Asn Glu Asp Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 100 105 110 Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 115 120 125 Gly Gly Gly Gly Ser His Ser Gln Ile Gln Leu Gln Gln Ser Gly Ala 130 135 140 Glu Leu Val Arg Pro Gly Ser Ser Val Lys Ile Ser Cys Lys Ala Ser 145 150 155 160 Gly Phe Ala Phe Ser Ser Tyr Trp Met Asn Trp Val Lys Gln Arg Pro 165 170 175 Gly Gln Gly Leu Glu Trp Ile Gly Gln Ile Tyr Pro Gly Asp Gly Asp 180 185 190 Thr Lys Tyr Asn Val Lys Phe Arg Gly Lys Ala Thr Leu Thr Ala Asp 195 200 205 Glu Ser Ser Ser Thr Ala Tyr Ile Gln Leu Thr Ser Leu Thr Ser Glu 210 215 220 Asp Ser Gly Val Tyr Phe Cys Ala Arg Lys Arg Ile Thr Ala Val Ile 225 230 235 240 Thr Thr Val Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser 245 250 255 Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile 260 265 270 Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala 275 280 285 Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 290 295 300 Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 305 310 315 320 Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile 325 330 335 Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp 340 345 350 Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 355 360 365 Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 370 375 380 Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 385 390 395 400 Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 405 410 415 Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 420 425 430 Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 435 440 445 Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 450 455 460 Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 465 470 475 480 Arg <210> 13 <211> 2560 <212> ADN <213> Secuencia artificial <220> <223> Secuencia artificial <400> 13 cgcctttttc cggagggtgg gggagaaccg tatataagtg cagtagtccg tgaacgttct 60 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc 120 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta 180 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc 240 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg 300 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat 360 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc 420 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg 480 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg 540 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat 600 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg 660 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg 720 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg 780 tgactccact gagtaccggg cgccgtccag gcacctcgat tagttctcgt gcttttggag 840 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg 900 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct 960 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct 1020 tccatttcag gtgtcatgag cctcatcccg aatggctctg cctgtcactg gactgctgct 1080 gtcactgggg ctgctgctgc acgccgcaag accaaccggt aacatcgtgc tgactcagag 1140 cccagcatcc ctggccgtct cactgggaca gcgggctacc atcagctgca gagcaagcga 1200 gtccgtggac aacttcggaa attcctttat gcactggtac cagcagaagt ctggccagcc 1260 ccctcgactg ctgatctata ttgcctctaa cctggagagt ggcgtgccag ctaggttctc 1320 tggcagtggg tcacgcacag actttaccct gacaattgat cccgtcgaag ccgacgatgc 1380 cgctacctac tattgccacc agaacaacga ggacccactg actttcggag cagggacaaa 1440 actggaactg aagggtggag gcgggagcgg aggaggcggg tcgggaggag gaggatcggg 1500 tggcggaggc tcacattctc agattcagct gcagcagtca ggggcagagc tggtgcggcc 1560 agggtcctca gtcaagatta gttgtaaggc ttcaggcttt gctttcagct cctactggat 1620 gaactgggtg aaacagcgac caggacaggg actggagtgg atcggacaga tctaccccgg 1680 agacggcgat acaaagtata atgtcaaatt tcggggcaag gccaccctga cagctgacga 1740 gtctagttca accgcctaca tccagctgac ttcactgacc agcgaagatt ccggcgtgta 1800 tttctgcgct cgaaagcgga tcactgcagt cattaccaca gtgtttgacg tgtggggggc 1860 aggaactacc gtgaccgtca gctccacaac tacgccggcg ccgagaccac ctacacctgc 1920 accaactatt gcctctcagc cactgagtct gcgccccgag gcatgtcgac ctgccgctgg 1980 cggggctgtg cacaccaggg gcctagactt cgcctgcgat atctatattt gggctccact 2040 ggcaggaacc tgtggcgtgc tgctgctgtc tctggtcatc acactgtact gcaaaagagg 2100 caggaagaaa ctgctgtata ttttcaagca gccctttatg agacctgtgc agacaactca 2160 ggaggaagac gggtgcagct gtaggttccc tgaggaagag gaaggaggct gtgagctgcg 2220 cgtgaaattt tctcggagtg cagatgcccc agcttaccag cagggccaga accagctgta 2280 taacgagctg aatctggggc ggagagagga atacgacgtg ctggataaga ggcgcgggcg 2340 agatccagaa atgggaggaa aaccccagcg acggaagaac cctcaggagg gactgtacaa 2400 tgaactgcag aaggacaaaa tggcagaggc ctattccgaa atcgggatga aaggagaaag 2460 aaggcgcggc aaggggcatg atggcctgta tcagggactg tcaaccgcaa caaaagatac 2520 ttatgatgct ctgcacatgc aggctctgcc cccgcggtaa 2560