US UPN-19-9075PCT_20221112 US US 63/278,775 2021-11-12 The Trustees of The University of Pennsylvania GENE THERAPY FOR TREATMENT OF MUCOPOLYSACCHARIDOSIS III A GENOTERAPIA PARA EL TRATAMIENTO DE LA MUCOPOLISACARIDOSIS III A 60 4738 DNA PAT misc_feature 1..4738 note vector genome CB7.CI.BiP.hSGSHcov1(A482Y-E488V).vIGF2.WPRE.4xmiR183.rBG genoma de vector CB7.CI.BiP.hSGSHcov1(A482Y-E488V).vIGF2.WPRE.4xmiR183.rBG repeat_region 1..130 note 5'ITR 5'ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo misc_feature 1934..3694 note BiP-hSGSHcoV1-A482Y_E488V_vIGF2 BiP-hSGSHcoV1-A482Y_E488V_vIGF2 misc_feature 3383..3385 note A482Y A482Y misc_feature 3401..3403 note E488V E488V misc_feature 3446..3688 note linker vIGF2 enlazador vIGF2 misc_feature 3707..4248 note WPRE element elemento WPRE misc_feature 4259..4280 note miRNA183 miARN183 misc_feature 4285..4306 note miRNA183 miARN183 misc_feature 4313..4334 note miRNA183 miARN183 misc_feature 4339..4360 note miRNA183 miARN183 regulatory 4394..4520 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 4609..4738 note 3' ITR 3' ITR source 1..4738 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataagagcttggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 4323 DNA PAT misc_feature 1..4323 note expression cassette CB7.CI.BiP.hSGSHcov1(A482Y-E488V).vIGF2.WPRE.4xmiR183.rBG casete de expresión CB7.CI.BiP.hSGSHcov1(A482Y-E488V).vIGF2.WPRE.4xmiR183.rBG regulatory 1..382 note promoter - CMV promotor - CMV regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta actin intron Intrón - intrón de beta actina de pollo misc_feature 3510..4051 note WPRE WPRE misc_feature 4062..4083 note miRNA183 miARN183 misc_feature 4088..4109 note miRNA183 miARN183 misc_feature 4116..4137 note miRNA183 miARN183 misc_feature 4142..4163 note miRNA183 miARN183 regulatory 4197..4323 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..4323 mol_type other DNA organism synthetic construct construcción sintética CDS 1737..3494 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 protein_id 3 translation ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataagagcttggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 585 AA PAT REGION 1..585 note Synthetic Construct Construcción sintética source 1..585 mol_type protein organism synthetic construct construcción sintética ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 4099 DNA PAT misc_feature 1..4099 note vector genome CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.rBG genoma de vector CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.rBG repeat_region 1..130 note 5' ITR 5' ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo misc_feature 1934..3700 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 misc_feature 3383..3385 note A482Y A482Y misc_feature 3401..3403 note E488V E488V misc_feature 3446..3688 note linker-vIGF2 enlazador-vIGF2 regulatory 3755..3881 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 3970..4099 note 3' ITR 3' ITR source 1..4099 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggtacctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 3684 DNA PAT misc_feature 1..3684 note expression cassette CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.rBG casete de expresión CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta actin promoter Intrón - promotor de beta actina de pollo misc_feature 3186..3188 note A482Y A482Y misc_feature 3204..3206 note E488V E488V misc_feature 3249..3491 note linker vIGF2 enlazador vIGF2 regulatory 3558..3684 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..3684 mol_type other DNA organism synthetic construct construcción sintética CDS 1737..3494 note BiP-hSGSHcoV1-A482Y_E488V_vIGF2 BiP-hSGSHcoV1-A482Y_E488V_vIGF2 protein_id 6 translation ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggtacctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 585 AA PAT REGION 1..585 note Synthetic Construct Construcción sintética source 1..585 mol_type protein organism synthetic construct construcción sintética ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 4652 DNA PAT misc_feature 1..4652 note vector genome CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.rBG genoma de vector CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.rBG repeat_region 1..130 note 5'ITR 5'ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta actin promoter Intrón - promotor de beta actina de pollo misc_feature 1934..3695 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 misc_feature 3383..3385 note A482Y A482Y misc_feature 3401..3403 note E488V E488V misc_feature 3446..3688 note linker vIGF2 enlazador vIGF2 misc_feature 3707..4248 note WPRE WPRE regulatory 4308..4434 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 4523..4652 note 3' ITR 3' ITR source 1..4652 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataagagcttggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 4237 DNA PAT misc_feature 1..4237 note expression cassette CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.rBG casete de expresión CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo misc_feature 3186..3188 note A482Y A482Y misc_feature 3204..3206 note E488V E488V misc_feature 3249..3491 note linker vIGF2 enlazador vIGF2 misc_feature 3510..4051 note WPRE WPRE regulatory 4111..4237 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..4237 mol_type other DNA organism synthetic construct construcción sintética CDS 1737..3494 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 protein_id 9 translation ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataagagcttggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 585 AA PAT REGION 1..585 note Synthetic Construct Construcción sintética source 1..585 mol_type protein organism synthetic construct construcción sintética ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 3862 DNA PAT misc_feature 1..3862 note vector genome CB7.CI.hSGSHcoV1.rBG genoma de vector CB7.CI.hSGSHcoV1.rBG repeat_region 1..130 note 5' ITR 5' ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta actin intron Intrón - intrón de beta actina de pollo misc_feature 1946..3451 note hSGSHcoV1 hSGSHcoV1 regulatory 3518..3644 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 3733..3862 note 3' ITR 3' ITR source 1..3862 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgcagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtgataaggtacctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 3447 DNA PAT misc_feature 1..3447 note expression cassette CB7.CI.hSGSHcoV1.rBG casete de expresión CB7.CI.hSGSHcoV1.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo regulatory 3321..3447 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..3447 mol_type other DNA organism synthetic construct construcción sintética CDS 1749..3254 note hSGSHcoV1 hSGSHcoV1 protein_id 12 translation MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgcagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtgataaggtacctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 502 AA PAT REGION 1..502 note Synthetic Construct Construcción sintética source 1..502 mol_type protein organism synthetic construct construcción sintética MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL 3944 DNA PAT misc_feature 1..3944 note vector genome CB7.CI.hSGSHcoV1-4xmiR183.rBG genoma de vector CB7.CI.hSGSHcoV1-4xmiR183.rBG repeat_region 1..130 note 5'ITR 5'ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note polyA_signal - CB promoter polyA_signal - promotor CB regulatory_class polyA_signal_sequence misc_feature 956..1928 note Intron - chicken beta-actin inron Intrón - intrón de beta actina de pollo misc_feature 1946..3451 note hSGSHcoV1 hSGSHcoV1 misc_feature 3465..3486 note miRNA183 miRNA183 misc_feature 3491..3512 note miRNA183 miRNA183 misc_feature 3519..3540 note miRNA183 miRNA183 misc_feature 3545..3566 note miRNA183 miRNA183 regulatory 3600..3726 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 3815..3944 note 3' ITR 3' ITR source 1..3944 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgcagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtgataagggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 3529 DNA PAT misc_feature 1..3529 note expression cassette CB7.CI.hSGSHcoV1-4xmiR183.rBG casete de expresión CB7.CI.hSGSHcoV1-4xmiR183.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta actin intron Intrón - intrón de beta actina de pollo misc_feature 3268..3289 note miRNA183 miRNA183 misc_feature 3294..3315 note miRNA183 miRNA183 misc_feature 3322..3343 note miRNA183 miRNA183 misc_feature 3348..3369 note miRNA183 miRNA183 regulatory 3403..3529 note polyA_signal - Rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..3529 mol_type other DNA organism synthetic construct construcción sintética CDS 1749..3257 note hSGSHcoV1 hSGSHcoV1 protein_id 15 translation MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgcagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtgataagggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 502 AA PAT REGION 1..502 note Synthetic Construct Construcción sintética source 1..502 mol_type protein organism synthetic construct construcción sintética MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL 1452 DNA PAT misc_feature 1..1452 note engineered nucleic acid sequence encoding mature hSGSH secuencia de ácido nucleico modificada genéticamente que codifica hSGSH madura source 1..1452 mol_type other DNA organism synthetic construct construcción sintética agagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactg 484 AA PAT REGION 1..484 note mature hSGSH hSGSH madura source 1..484 mol_type protein organism synthetic construct construcción sintética RARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL 1506 DNA PAT misc_feature 1..1506 note engineered nucleic acid sequence encoding BiP.hSGSH secuencia de ácido nucleico modificada genéticamente que codifica BiP.hSGSH source 1..1506 mol_type other DNA organism synthetic construct construcción sintética atgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggaccccacaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtgataa 500 AA PAT REGION 1..500 note BiP.hSGSH BiP.hSGSH source 1..500 mol_type protein organism synthetic construct construcción sintética MKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL 1749 DNA PAT misc_feature 1..1749 note engineered nucleic acid sequence encoding BiP.hSGSH.vIGF2 secuencia de ácido nucleico modificada genéticamente que codifica BiP.hSGSH.vIGF2 source 1..1749 mol_type other DNA organism synthetic construct construcción sintética atgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggaccccacaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgacggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataa 581 AA PAT REGION 1..581 note BiP.hSGSH.vIGF2 BiP.hSGSH.vIGF2 source 1..581 mol_type protein organism synthetic construct construcción sintética MKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 1452 DNA PAT misc_feature 1..1452 note engineered nucleic acid sequence encoding mature hSGSH.A482Y-E488V secuencia de ácido nucleico modificada genéticamente que codifica hSGSH.A482Y-E488V madura source 1..1452 mol_type other DNA organism synthetic construct construcción sintética agagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactg 484 AA PAT REGION 1..484 note mature hSGSH.A482Y-E488V hSGSH.A482Y-E488V madura source 1..484 mol_type protein organism synthetic construct construcción sintética RARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNEL 1500 DNA PAT misc_feature 1..1500 note engineered nucleic acid sequence encoding BiP.hSGSH.A482Y-E488V secuencia de ácido nucleico modificada genéticamente que codifica BiP.hSGSH.A482Y-E488V source 1..1500 mol_type other DNA organism synthetic construct construcción sintética atgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactg 500 AA PAT REGION 1..500 note BiP.hSGSH.A482Y-E488V BiP.hSGSH.A482Y-E488V source 1..500 mol_type protein organism synthetic construct construcción sintética MKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNEL 1749 DNA PAT misc_feature 1..1749 note engineered nucleic acid sequence encoding BiP.hSGSH.A482Y-E488V.vIGF2 secuencia de ácido nucleico modificada genéticamente que codifica BiP.hSGSH.A482Y-E488V.vIGF2 source 1..1749 mol_type other DNA organism synthetic construct construcción sintética atgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataa 581 AA PAT REGION 1..581 note BiP.hSGSH.A482Y-E488V.vIGF2 BiP.hSGSH.A482Y-E488V.vIGF2 source 1..581 mol_type protein organism synthetic construct construcción sintética MKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 22 DNA PAT misc_feature 1..22 note miRNA183 targeting sequence secuencia de direccionamiento de miARN183 source 1..22 mol_type other DNA organism synthetic construct construcción sintética agtgaattctaccagtgccata 48 DNA PAT misc_feature 1..48 note engineered nucleic acid sequence encoding BiP peptide secuencia de ácido nucleico modificada genéticamente que codifica péptido BiP source 1..48 mol_type other DNA organism synthetic construct construcción sintética atgaagctgtctctggtggctgctatgctgctgctcctgtctgccgcc 16 AA PAT REGION 1..16 note BiP peptide Péptido BiP source 1..16 mol_type protein organism synthetic construct construcción sintética MKLSLVAAMLLLLSAA 54 DNA PAT misc_feature 1..54 note engineered nucleic acid sequence encoding hSGSH native signal peptide secuencia de ácido nucleico modificada genéticamente que codifica el péptido señal nativo de hSGSH source 1..54 mol_type other DNA organism synthetic construct construcción sintética atgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgc 18 AA PAT REGION 1..18 note hSGSH native signal peptide péptido señal nativo de hSGSH source 1..18 mol_type protein organism synthetic construct construcción sintética MSCPVPACCALLLVLGLC 246 DNA PAT misc_feature 1..246 note engineered nucleic acid sequence encoding vIGF2 peptide secuencia de ácido nucleico modificada genéticamente que codifica el péptido vIGF2 source 1..246 mol_type other DNA organism synthetic construct construcción sintética agacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatga 81 AA PAT REGION 1..81 note vIGF2 peptide péptido vIGF2 source 1..81 mol_type protein organism synthetic construct construcción sintética RPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 1509 DNA PAT misc_feature 1..1509 note engineered nucleic acid sequence encoding hSGSH comprising native signal peptide secuencia de ácido nucleico modificada genéticamente que codifica hSGSH que comprende el péptido señal nativo source 1..1509 mol_type other DNA organism synthetic construct construcción sintética atgtcttgtcctgtgcctgcctgttgtgccctgctgctggttctgggactgtgcagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgcgctcctgatggcgtgctggaagagaagctgtcccctcagtgtcagcccctgcacaatgaactgtga 502 AA PAT REGION 1..502 note hSGSH comprising native signal peptide hSGSH que comprende el péptido señal nativo source 1..502 mol_type protein organism synthetic construct construcción sintética MSCPVPACCALLLVLGLCRARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL 4901 DNA PAT misc_feature 1..4901 note vector genome CB7.CI.Bip.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.4xmiR183.rBG . genoma de vector CB7.CI.Bip.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.4xmiR183.rBG . repeat_region 1..130 note 5' ITR 5' ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo misc_feature 1934..3694 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 misc_feature 3383..3385 note A482Y A482Y misc_feature 3401..3403 note E488V E488V misc_feature 3446..3688 note linker-vIGF2 enlazador-vIGF2 misc_feature 3701..4242 note WPRE WPRE misc_feature 4302..4325 note miR182 miR182 misc_feature 4330..4353 note miR182 miR182 misc_feature 4360..4383 note miR182 miR182 misc_feature 4390..4413 note miR182 miR182 misc_feature 4422..4443 note miR183 miR183 misc_feature 4448..4469 note miR183 miR183 misc_feature 4476..4497 note miR183 miR183 misc_feature 4502..4523 note miR183 miR183 regulatory 4557..4683 note polyA_signal - Rabbit beta globin polyA polyA_signal - beta globina poliA de conejo regulatory_class polyA_signal_sequence repeat_region 4772..4901 note 3" ITR 3" ITR source 1..4901 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccagtgtgagttctaccattgccaaaggatagtgtgagttctaccattgccaaacacgtgagtgtgagttctaccattgccaaagcatgcagtgtgagttctaccattgccaaagcggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 4486 DNA PAT misc_feature 1..4486 note expression cassette CB7.CI.Bip.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.4xmiR183.rBG casete de expresión CB7.CI.Bip.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.4xmiR183.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB pormoter promotor - promotor CB regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta actin intron Intrón - intrón de beta actina de pollo misc_feature 3186..3188 note A482Y A482Y misc_feature 3204..3206 note E488V E488V misc_feature 3249..3491 note linker vIGF2 enlazador vIGF2 misc_feature 3504..4045 note WPRE element elemento WPRE misc_feature 4105..4128 note miR182 miR182 misc_feature 4133..4156 note miR182 miR182 misc_feature 4163..4186 note miR182 miR182 misc_feature 4193..4216 note miR182 miR182 misc_feature 4225..4246 note miR183 miR183 misc_feature 4251..4272 note miR183 miR183 misc_feature 4279..4300 note miR183 miR183 misc_feature 4305..4326 note miR183 miR183 regulatory 4360..4486 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..4486 mol_type other DNA organism synthetic construct construcción sintética CDS 1737..3494 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 protein_id 39 translation ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccagtgtgagttctaccattgccaaaggatagtgtgagttctaccattgccaaacacgtgagtgtgagttctaccattgccaaagcatgcagtgtgagttctaccattgccaaagcggccgcagtgaattctaccagtgccatacgatagtgaattctaccagtgccataaccggtagtgaattctaccagtgccatatcacagtgaattctaccagtgccatactcgaggacggggtgaactacgcctgaggatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 585 AA PAT REGION 1..585 note Synthetic Construct Construcción sintética source 1..585 mol_type protein organism synthetic construct construcción sintética ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 4769 DNA PAT misc_feature 1..4769 note vector genome CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.rBG genoma de vector CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.rBG repeat_region 1..130 note 5' ITR 5' ITR regulatory 198..579 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 582..863 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 956..1928 note Intron - chicken beta actin intron Intrón - intrón de beta actina de pollo misc_feature 1934..3694 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 misc_feature 3383..3385 note A482Y A482Y misc_feature 3401..3403 note E488V E488V misc_feature 3701..4242 note WPRE element elemento WPRE misc_feature 4302..4325 note miR182 miR182 misc_feature 4330..4353 note miR182 miR182 misc_feature 4360..4383 note miR182 miR182 misc_feature 4390..4413 note miR182 miR182 regulatory 4425..4551 note polyA_signal - rabbit beta globin polyA (RBG) polyA_signal - beta globina poliA de conejo (RBG) regulatory_class polyA_signal_sequence repeat_region 4640..4769 note 3' ITR 3' ITR source 1..4769 mol_type other DNA organism synthetic construct construcción sintética regulatory 198..863 note CB7 promoter promotor CB7 regulatory_class promoter ctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctaccagggtaatggggatcctctagaactatagctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccagtgtgagttctaccattgccaaaggatagtgtgagttctaccattgccaaacacgtgagtgtgagttctaccattgccaaagcatgcagtgtgagttctaccattgccaaagcggccgatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaagcaattcgttgatctgaatttcgaccacccataatacccattaccctggtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcag 4354 DNA PAT misc_feature 1..4354 note expression cassette CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.rBG casete de expresión CB7.CI.BiP.hSGSHcov1-A482Y_E488V.vIGF2.WPRE.4xmiR182.rBG regulatory 1..382 note promoter - CMV IE promotor - CMV IE regulatory_class enhancer regulatory 385..666 note promoter - CB promoter promotor - CB promotor regulatory_class promoter misc_feature 759..1731 note Intron - chicken beta-actin intron Intrón - intrón de beta actina de pollo misc_feature 3186..3188 note A482Y A482Y misc_feature 3204..3206 note E488V E488V misc_feature 3249..3491 note linker vIGF2 enlazador vIGF2 misc_feature 3504..4045 note WPRE element elemento WPRE misc_feature 4105..4128 note miR182 miR182 misc_feature 4133..4156 note miR182 miR182 misc_feature 4163..4186 note miR182 miR182 misc_feature 4193..4216 note miR182 miR182 regulatory 4228..4354 note polyA_signal - rabbit globin polyA polyA_signal - globina poliA de conejo regulatory_class polyA_signal_sequence source 1..4354 mol_type other DNA organism synthetic construct construcción sintética CDS 1737..3494 note BiP-hSGSHcoV1-A482Y-E488V-vIGF2 BiP-hSGSHcoV1-A482Y-E488V-vIGF2 protein_id 42 translation ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE regulatory 1..666 note CB7 promoter promotor CB7 regulatory_class promoter ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgctagcgccaccatgaagctgtctctggtggctgctatgctgctgctcctgtctgccgccagagccagacctagaaacgctctgctgctgctcgctgacgacggcggatttgagagcggcgcctacaacaacagcgccattgccacacctcacctggacgccctggccagaagaagcctgctgttcagaaacgccttcaccagcgtgtccagctgcagcccttctagagcctctctgctgactggactgccccagcaccagaacgggatgtacggcctgcaccaggacgtgcaccacttcaacagcttcgacaaagtgcggagcctgcctctgcttctgtctcaagccggcgtcagaacaggcatcatcggcaagaaacacgtgggccccgagacagtgtaccccttcgatttcgcctacaccgaagagaacggcagcgtgctgcaagtgggcagaaacatcacccggatcaagctgctcgtgcggaagttcctgcagacccaggacgacagacccttcttcctgtacgtggccttccacgatcctcacagatgcggccatagccagcctcagtacggcaccttctgcgagaagtttggcaacggcgagagcggcatgggcagaatccctgattggacccctcaggcctacgatcccctggacgtgctggtgccttacttcgtgcctaacacaccagccgccagagccgatctggccgctcagtatacaaccgtgggaagaatggaccaaggcgtcggcctggttctgcaagagcttagagatgccggcgtgctgaacgacaccctggtcatctttaccagcgacaacggcatcccctttccatctggccggaccaatctgtactggcctggaacagctgagcccctgctggtgtctagccctgagcaccctaagagatggggccaagtgtctgaggcctacgtgtccctgctggatctgacccctaccatcctggactggttcagcatcccctatcctagctacgccatcttcggcagcaagaccatccacctgaccggcagatctctgctgccagctctggaagctgaacctctgtgggccacagtgtttggcagccagtctcaccacgaagtgaccatgagctaccccatgagaagcgtgcagcaccggcacttcagactggtgcacaacctgaacttcaagatgccctttccaatcgaccaggacttctacgtgtccccaaccttccaggacctgctgaacagaaccacagccggccaacctaccggctggtacaaggacctgcggcactactactatagagccaggtgggagctgtacgaccggtccagagatccccacgagacacagaacctggccaccgatcctagattcgcccagctgctggaaatgctgagagatcagctggccaagtggcagtgggagacacacgatccttgggtctgctatcctgatggcgtgctagtggagaagctgtcccctcagtgtcagcccctgcacaatgaactgagacctagagctgtgcctacacagggtggcggaggatctggcggaggcggatctagcagaacactttgtggcggagagctggtggacaccctgcagtttgtgtgtggcgacagaggcttcctgttcagcagacctgccagccgggtttccagacggtctagaggaatcgtggaagagtgctgcttcagaagctgcgatctggccctgctggaaacctactgtgccacaccagccagatctgaatgataaggatccaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgagatctctagagtcgacccgggcggcctcgaggacggggtgaactacgcctgaggatccagtgtgagttctaccattgccaaaggatagtgtgagttctaccattgccaaacacgtgagtgtgagttctaccattgccaaagcatgcagtgtgagttctaccattgccaaagcggccgatccgatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 585 AA PAT REGION 1..585 note Synthetic Construct Construcción sintética source 1..585 mol_type protein organism synthetic construct construcción sintética ASATMKLSLVAAMLLLLSAARARPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCYPDGVLVEKLSPQCQPLHNELRPRAVPTQGGGGSGGGGSSRTLCGGELVDTLQFVCGDRGFLFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPARSE 542 DNA PAT misc_feature 1..542 note WPRE element (mut) elemento WPRE (mut) source 1..542 mol_type other DNA organism synthetic construct construcción sintética aatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcg 666 DNA PAT misc_feature 1..666 note CB7 promoter promotor CB7 source 1..666 mol_type other DNA organism synthetic construct construcción sintética ctagtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgg 973 DNA PAT misc_feature 1..973 note Chicken beta actin intron Intrón de beta actina de pollo source 1..973 mol_type other DNA organism synthetic construct construcción sintética gtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaag 127 DNA PAT misc_feature 1..127 note rabbit beta globin polyA (RBG) beta globin poliA de conejo (RBG) source 1..127 mol_type other DNA organism synthetic construct construcción sintética gatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcg 24 DNA PAT misc_feature 1..24 note miR182 targeting sequence secuencia de direccionamiento miR182 source 1..24 mol_type other DNA organism synthetic construct construcción sintética agtgtgagttctaccattgccaaa 2211 DNA PAT source 1..2211 mol_type other DNA organism adeno-associated virus hu68 virus adenoasociado hu68 atggctgccgatggttatcttccagattggctcgaggacaacctcagtgaaggcattcgcgagtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaacgctcggggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaagggggagccggtcaacgaagcagacgcggcggccctcgagcacgacaaggcctacgaccagcagctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccaggagcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaaaagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcctccgtgggtattggcaaatcgggtgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccccgaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacccgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcaaagactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgctaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtacgggtatctaacgcttaatgatggaagccaagccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgctaagaacgggtaacaacttccagttcagctacgagtttgagaacgtacctttccatagcagctatgctcacagccaaagcctggaccgactcatgaatccactcatcgaccaatacttgtactatctctcaaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacccagcaacatggctgtccagggaagaaactacatacctggacccagctaccgacaacaacgtgtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttcttgggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaagaaggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactggaagagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactaccaacccagtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcccaagcacaggcgcagaccggctgggttcaaaaccaaggaatacttccgggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctcacacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcctcctcagatcctcatcaaaaacacacctgtacctgcggatcctccaacggctttcaacaaggacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagattgagtgggagctgcagaaggaaaacagcaagcgctggaacccggagatccagtacacttccaactattacaagtctaataatgttgaatttgctgttaatactgaaggtgtttattctgaaccccgccccattggcaccagatacctgactcgtaatctgtaa 2211 DNA PAT source 1..2211 mol_type other DNA note adeno-associated virus hu68 engineered nucleic acid sequence secuencia de ácido nucleico modificada genéticamente del virus adenoasociado hu68 organism synthetic construct construcción sintética atggctgccgatggttatcttccagattggctcgaggacaacctcagtgaaggcattcgcgagtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaacgctcggggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaagggggagccggtcaacgaagcagacgcggcggccctcgagcacgacaaggcctacgaccagcagctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccaggagcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaaaagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcctccgtgggtattggcaaatcgggtgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccccgaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacccgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcaaagactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgctaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtatggatacctcaccctgaacgacggcagtcaggcggtgggccgctcatccttctactgcctggagtacttcccttcgcagatgctgaggactggcaacaacttccagttcagctacgagttcgagaacgtccctttccacagcagctacgcccacagccagagtttggaccgcttgatgaaccctctgatcgaccagtacctgtactacctgtcaaagacgatcaacggttctggccagaaccagcagacgctgaagttcagcgtggccgggcctagcaacatggccgtccagggcagaaactacatccctgggcccagctaccggcagcagagagtctcaaccactgtgactcagaacaacaacagtgagttcgcctggcctggcgccagctcttgggccctcaacggccgcaactcgctgatgaacccaggcccagccatggccagtcacaaggagggcgaggaccgtttcttccctttgtctggctctctgatcttcggcaagcaggggaccggcagagacaacgtggacgcggacaaggtcatgatcacgaacgaggaggagatcaagaccaccaaccctgtggcaaccgagtcctacggccaggtggcaaccaaccaccagagcgcccaggcacaggcgcagactggctgggtccagaaccaggggatcctgcctggcatggtgtggcaggaccgtgacgtgtacctgcagggccctatctgggcaaagatccctcacacggacggcaacttccacccttctcctctgatgggcggcttcggcatgaagcacccgcctcctcagatcctcatcaagaacactccggtcccggcagaccctccgacggccttcaacaaggacaagctgaactcattcatcactcagtactccactggccaggtcagcgtggagatcgagtgggagctgcagaaggagaacagcaagcgttggaacccagagatccagtacacttccaactactacaagtctaacaacgtggagttcgccgtcaacactgagggtgtgtacagtgagcctcgccctatcggcacccggtacctcacccgaaacttgtga 736 AA PAT source 1..736 mol_type protein organism adeno-associated virus hu68 virus adenoasociado hu68 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSVGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL 2211 DNA PAT source 1..2211 mol_type other DNA organism adeno-associated virus rh91 virus adenoasociado rh91 atggctgccgatggttatcttccagattggctcgaggacaacctctctgagggcattcgcgagtggtgggcgctgaaacctggagccccgaaacccaaagccaaccagcaaaagcaggacgacggccggggtctggtgcttcctggctacaagtacctcggacccttcaacggactcgacaagggggagcccgtcaacgcggcggacgcagcggccctcgagcacgacaaggcctacgaccagcagctcaaagcgggtgacaatccgtacctgcggtataaccacgccgacgccgagtttcaggagcgtctgcaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaagaagcgggttctcgaaccttttggtctggttgaggaagcagctaagacggctcctggaaagaaacgtccggtagagcagtcgccccaagaaccagactcctcctcgggcattggcaaatcaggccagcagcccgccaaaaagagactcaatttcggtcagactggcgactcagagtcagtccccgaccctcaacctctcggagaacctccagaaacccccgctgctgtgggacctactacaatggcttcaggcggtggcgcaccaatggcagacaataacgaaggcgccgacggagtgggtaatgcctcaggaaattggcattgcgattccacatggctgggcgacagagtcatcaccaccagcacccgaacctgggcccttcctacctacaacaaccacctctacaagcaaatctccagcgcttcaacgggggccagtaacgacaaccactactttggctacagcaccccctgggggtattttgatttcaacagattccactgccacttctcaccacgtgactggcagcgactcattaacaacaactggggattccggcccaagagactcaacttcaagctcttcaacatccaggtcaaggaggtcacgacgaatgatggcgtcacaaccatcgctaataaccttaccagcacggttcaagtgttctcggactcggagtaccagctgccgtacgtcctcggttctgcgcaccagggctgcctccctccgttcccggcggacgtattcatgattcctcagtacggctacctaacgctcaacaatggcagccaggccgtaggacgttcatccttttattgcctggaatatttcccatctcaaatgctgagaacgggcaacaactttaccttcagctacacctttgaagatgtgcctttccacagcagttacgcgcacagccagagcctggacaggctaatgaatcctctaatcgaccagtacctgtattacctaaacagaactcagaatcaatccggaagtgcacaaaacaaggacttgctgtttagccgggggtctccagctggcatgtctgttcagcccaaaaactggctacccgggccctgttaccgacagcagcgtgtttctaaaacaaaaacagacaacaacaacagcaactttacctggactggtgcctccaaatacaatctgaacggacgtgaatccatcattaaccctggcaccgctatggcatcccacaaggacgacgaagacaaattttttcccatgagcggtgttatgatttttggcaaagaaaatgcaggagcatcaaacactgcattagacaatgttatgattacagatgaagaggaaattaaagctaccaaccccgtggccaccgagagatttggaactgtggcagtcaatctccaaagcagcaatacagaccctgcaacaggagacgtgcatgtcatgggggctttacctggcatggtgtggcaagacagagacgtgtacctgcagggtcccatttgggccaagattcctcacacggatggacactttcacccgtctcctcttatgggcggctttggacttaagcacccgcctcctcagatcctcatcaaaaacacgcctgttcctgcgaatcctccggcagagttttcggctacaaagtttgcttcattcatcacccagtactccacaggacaagtgagcgtggaaattgaatgggagctgcagaaagaaaacagtaagcgctggaatcctgaagtgcagtacacctccaactacgcgaaatctgccaacgttgatttcactgtggacaacaatggactttatactgagcctcgccccattggcacccgttaccttacccgtcccctttaa 2211 DNA PAT source 1..2211 mol_type other DNA note adeno-associated virus rh91 engineered nucleic acid sequence secuencia de ácido nucleico modificada genéticamente del virus adenoasociado rh91 organism synthetic construct construcción sintética atggctgctgacggttatcttccagattggctcgaggacaacctttctgaaggcattcgtgagtggtgggctctgaaacctggagcccctaaacccaaagcgaaccaacaaaagcaggacgacggccggggtcttgtgcttccgggttacaaatacctcggacccttcaacggactcgacaaaggagagccggtcaacgcggcggacgcggcagccctcgaacacgacaaagcttacgaccagcagctcaaggccggtgacaacccgtacctccggtacaaccacgccgacgccgagtttcaggagcgtcttcaagaagatacgtcttttgggggcaaccttggcagagcagtcttccaggccaaaaagagggttcttgagccttttggtctggttgaggaagcagctaaaacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcatcatctggtattggcaaatcgggccagcagcctgccaaaaaaagactaaatttcggtcagactggcgactcagagtcagtccccgaccctcaacctctcggagaacctccagaaacccccgctgctgtgggacctactacaatggcttcaggcggtggcgcaccaatggcagacaataacgaaggcgccgacggagtgggtaatgcctcaggaaattggcattgcgattccacatggctgggcgacagagtcatcaccaccagcacccgaacctgggcccttcctacctacaacaaccacctctacaagcaaatctccagcgcttcaacgggggccagtaacgacaaccactactttggctacagcaccccctgggggtattttgatttcaacagattccactgccacttctcaccacgtgactggcagcgactcattaacaacaactggggattccggcccaagagactcaacttcaagctcttcaacatccaggtcaaggaggtcacgacgaatgatggcgtcacaaccatcgctaataaccttaccagcacggttcaagtgttctcggactcggagtaccagctgccgtacgtcctcggttctgcgcaccagggctgcctccctccgttcccggcggacgtattcatgattcctcagtatggatacctcaccctgaacaacggaagtcaagcggtgggacgctcatccttttactgcctggagtacttcccttcgcagatgctaaggactggaaataacttcaccttcagctataccttcgaggatgtaccttttcacagcagctacgctcacagccagagtttggatcgcttgatgaatcctcttattgatcagtatctgtactacctgaacagaacgcaaaatcaatctggaagtgcacaaaacaaggacctgctttttagccgggggtctcctgctggcatgtctgttcagcccaaaaattggctacctgggccctgctaccggcaacagagagtttcaaagactaaaacagacaacaacaacagtaactttacctggacaggtgccagcaaatataatctcaatggccgcgaatcgatcattaatccaggaaccgctatggccagtcacaaggacgatgaagacaaatttttccctatgagcggcgttatgatatttggcaaagaaaatgcaggagcaagtaacactgcattagataatgtaatgattacggatgaagaagagattaaagctaccaatcctgtggcaacagagagatttggaactgtggcagtcaacttgcagagctcaaatacagaccccgcaactggagacgtccatgtcatgggggccttacctggcatggtgtggcaagatcgtgacgtgtaccttcaaggacctatctgggcaaagattcctcacacggatggacactttcatccttctcctctgatgggaggctttggactgaaacatccgcctcctcaaatcctcatcaaaaatactccggtaccggcaaatcctccggcagagttcagcgctacaaagtttgcttcatttatcactcagtactccactggacaggtcagcgtggaaattgagtgggagctacagaaagaaaacagcaaacgttggaatccagaggtgcagtacacttccaactacgcgaagtctgccaatgtggactttactgtagacaacaatggtctttatactgaacctcgccctattggaacccggtatctcacacgacccttgtaa 736 AA PAT source 1..736 mol_type protein organism adeno-associated virus rh91 virus adenoasociado rh91 MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGLVLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLRYNHADAEFQERLQEDTSFGGNLGRAVFQAKKRVLEPFGLVEEAAKTAPGKKRPVEQSPQEPDSSSGIGKSGQQPAKKRLNFGQTGDSESVPDPQPLGEPPETPAAVGPTTMASGGGAPMADNNEGADGVGNASGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISSASTGASNDNHYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTTNDGVTTIANNLTSTVQVFSDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLNRTQNQSGSAQNKDLLFSRGSPAGMSVQPKNWLPGPCYRQQRVSKTKTDNNNSNFTWTGASKYNLNGRESIINPGTAMASHKDDEDKFFPMSGVMIFGKENAGASNTALDNVMITDEEEIKATNPVATERFGTVAVNLQSSNTDPATGDVHVMGALPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLMGGFGLKHPPPQILIKNTPVPANPPAEFSATKFASFITQYSTGQVSVEIEWELQKENSKRWNPEVQYTSNYAKSANVDFTVDNNGLYTEPRPIGTRYLTRPL 2211 DNA PAT source 1..2211 mol_type other DNA organism adeno-associated virus hu95 virus adenoasociado hu95 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcgagtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaacgctcggggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaagggggagccggtcaacgcagcagacgcggcggccctcgagcacgacaaggcctacgaccagcagctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagtttcaggagcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaaaagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcctccgcgggtattggcaaatcgggtgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccccgaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacccgaacctgggccctgcccacctacaataatcacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgctaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagccaagccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgcttcgtaccggaaacaactttaccttcagctacgagtttgagaacgtacctttccatagcagctatgctcacagccaaagcctggaccgtctcatgaatccactcatcgaccaatacttgtactatctctcaaagactattaacggttctggacagaatcaacaaacgctaaaattcagtgtggccggacccagcaacatggcagtccagggaagaaactacatacctggacccagctaccgacaacaacgtgtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttcttgggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaagaaggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactggaagagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactactaacccggtagcaacggagtcttatggacaagtggccacaaaccaccagagtgcccaagcacaggcgcagaccggctgggttcaaaaccaaggaatacttccgggtatggtttggcaggacagagatgtgtacctgcaaggacccatttgggccaaaattcctcacacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcctcctcagatcctcatcaaaaacacacctgtacctgcggatcctccaacggctttcaacaaggacaagctgaactctttcatcacccagtattctactggccaagttagcgtggagattgagtgggagctgcagaaggaaaacagcaagcgctggaacccggagatccagtacacttccaactattacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgccccattggcaccagatacctgactcgtaatctgtaa 2211 DNA PAT source 1..2211 mol_type other DNA note adeno-associated virus hu95 engineered nucleic acid sequence secuencia de ácido nucleico modificada genéticamente del virus adenoasociado hu95 organism synthetic construct construcción sintética atggctgctgacggttatcttccagattggctcgaggacaacctttctgaaggcattcgtgagtggtgggctctgaaacctggagcccctcaacccaaagcgaaccaacaacaccaggacaacgctcggggtcttgtgcttccgggttacaaatacctcggacccggtaacggactcgacaaaggagagccggtcaacgcagcggacgcggcagccctcgaacacgacaaagcctacgaccagcagctcaaggccggtgacaacccgtacctcaagtacaaccacgccgacgccgagtttcaggagcgtcttaaagaagatacgtcttttgggggcaaccttggcagagcagtcttccaggccaaaaagaggcttcttgagcctcttggtctggttgaggaagcagctaaaacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcatcagcgggtattggcaaatcgggcgcacagcctgccaaaaaaagactaaatttcggtcagactggcgacacagagtcagtcccagaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacccgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtatggatacctcaccctgaacgatggaagtcaagcggtgggacgctcatccttttactgcctggagtacttcccttcgcagatgctaaggactggaaataacttcaccttcagctatgagttcgagaacgtaccttttcacagcagctacgctcacagccagagtttggatcgcttgatgaatcctcttattgatcagtatctgtactacctgtcaaagacgattaacggttctggacagaaccaacaaacgctgaaatttagcgtggctgggcctagcaacatggctgtccagggaagaaattacatacctgggcccagctaccggcaacagagagtctcaaccactgtgactcaaaacaacaacagtgaatttgcttggcctggagccagctcttgggctctcaatggccgcaattcgctgatgaatccaggaccagctatggccagtcacaaggaaggagaagaccgttttttccctttgtctggctctctaatatttggcaaacaagggacaggaagagacaacgtggatgcggataaagtaatgattacgaacgaagaagagattaaaaccaccaatcctgtggcaacagagtcctatggacaagtggcaacaaaccaccagagcgcccaagcacaggcgcagactggctgggtccaaaaccaggggatattacctggcatggtgtggcaagatcgtgacgtgtaccttcaaggacctatctgggcaaagattcctcacacggatggaaactttcatccttctcctctgatgggaggctttggaatgaaacatccgcctcctcaaatcctcatcaaaaatactccggtaccggcagatcctccgacggccttcaacaaggacaagctgaactcatttatcactcagtactccactggacaggtcagcgtggaaattgagtgggagctacagaaagaaaacagcaaacgttggaatccagagattcagtacacttccaactactacaagtctaataatgtggaatttgctgtaaatactgaaggtgtttatagtgaacctcgccctattggaacccggtatctcacacgaaacttgtaa 736 AA PAT source 1..736 mol_type protein organism adeno-associated virus hu95 virus adenoasociado hu95 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL 2211 DNA PAT source 1..2211 mol_type other DNA organism adeno-associated virus hu96 virus adenoasociado hu96 atggctgccgatggttatcttccagattggctcgaggacaaccttagtgaaggaattcgcgagtggtgggctttgaaacctggagcccctcaacccaaggcaaatcaacaacatcaagacaacgctcggggtcttgtgcttccgggttacaaataccttggacccggcaacggactcgacaagggggagccggtcaacgcagcagacgccgcggccctcgagcacgacaaggcctacgaccagcagctcaaggccggagacaacccgtacctcaagtacaaccacgccgacgccgagttccaggagcggctcaaagaagatacgtcttttgggggcaacctcgggcgagcagtcttccaggccaaaaagaggcttcttgaacctcttggtctggttgaggaagcggctaagacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcctccgagggtattggcaaatcgggtgcacagcccgctaaaaagagactcaatttcggtcagactggcgacacagagtcagtccccgaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagttatcaccaccagcacccgaacctgggccctgcccacctacaacaaccacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtacgggtatctgacgcttaatgatggaagccaggccgtgggtcgttcgtccttttactgcctggaatatttcccgtcgcaaatgcttcgtaccggaaacaactttaccttcagctacgagtttgagaacgtacctttccatagcagctacgctcacagccaaagcctggaccgactaatgaatccactcatcgaccaatacttgtactatctctcaaagactattaacggttctgggcagaatcaacaaacgctaaaattcagtgtggccggacccagcaacatggcagtccagggaagaaactacatacctggacccatctaccgacaacaacgtgtctcaaccactgtgactcaaaacaacaacagcgaatttgcttggcctggagcttcttcttgggctctcaatggacgtaatagcttgatgaatcctggacctgctatggccagccacaaagaaggagaggaccgtttctttcctttgtctggatctttaatttttggcaaacaaggaactggaagagacaacgtggatgcggacaaagtcatgataaccaacgaagaagaaattaaaactaccaacccggtagcaacggagtcctatggacaagtggccacaaaccaccagagtgcccaagcacaggcgcaaacaggctgggttcaaaaccaaggaatacttccgggtatggtttggcaagacagagatgtgtacctgcaaggacccatttgggccaaaattcctcacacggacggcaactttcacccttctccgctgatgggagggtttggaatgaagcacccgcctcctcagatcctcatcaaaaacacacctgtacctgcggatcctccaacggctttcaacaaggacaagctgaactctttcatcacccagtattctactggccaagtcagcgtggagattgagtgggagctgcagaaggaaaacagcaagcgctggaacccggagatccagtacacttccaactattacaagtctaataatgttgaatttgctgttaatactgaaggtgtatatagtgaaccccgccccattggcaccagatacctgactcgtaatctgtaa 2211 DNA PAT source 1..2211 mol_type other DNA note adeno-associated virus hu96 engineered nucleic acid sequence secuencia de ácido nucleico modificada genéticamente del virus adenoasociado hu96 organism synthetic construct construcción sintética atggctgctgacggttatcttccagattggctcgaggacaacctttctgaaggcattcgtgagtggtgggctctgaaacctggagcccctcaacccaaagcgaaccaacaacaccaggacaacgctcggggtcttgtgcttccgggttacaaatacctcggacccggtaacggactcgacaaaggagagccggtcaacgcagcggacgcggcagccctcgaacacgacaaagcctacgaccagcagctcaaggccggtgacaacccgtacctcaagtacaaccacgccgacgccgagtttcaggagcgtcttaaagaagatacgtcttttgggggcaaccttggcagagcagtcttccaggccaaaaagaggcttcttgagcctcttggtctggttgaggaagcagctaaaacggctcctggaaagaagaggcctgtagagcagtctcctcaggaaccggactcatcagagggtattggcaaatcgggcgcacagcctgccaaaaaaagactaaatttcggtcagactggcgacacagagtcagtcccagaccctcaaccaatcggagaacctcccgcagccccctcaggtgtgggatctcttacaatggcttcaggtggtggcgcaccagtggcagacaataacgaaggtgccgatggagtgggtagttcctcgggaaattggcattgcgattcccaatggctgggggacagagtcatcaccaccagcacccgaacctgggccctgcccacctacaacaatcacctctacaagcaaatctccaacagcacatctggaggatcttcaaatgacaacgcctacttcggctacagcaccccctgggggtattttgacttcaacagattccactgccacttctcaccacgtgactggcagcgactcatcaacaacaactggggattccggcctaagcgactcaacttcaagctcttcaacattcaggtcaaagaggttacggacaacaatggagtcaagaccatcgccaataaccttaccagcacggtccaggtcttcacggactcagactatcagctcccgtacgtgctcgggtcggctcacgagggctgcctcccgccgttcccagcggacgttttcatgattcctcagtatggatacctcaccctgaacgatggaagtcaagcggtgggacgctcatccttttactgcctggagtacttcccttcgcagatgctaaggactggaaataacttcaccttcagctatgagttcgagaacgtaccttttcacagcagctacgctcacagccagagtttggatcgcttgatgaatcctcttattgatcagtatctgtactacctgtcaaagacgattaacggttctggacagaaccaacaaacgctgaaatttagcgtggctgggcctagcaacatggctgtccagggaagaaattacatacctgggcccatctaccggcaacagagagtctcaaccactgtgactcaaaacaacaacagtgaatttgcttggcctggagccagctcttgggctctcaatggccgcaattcgctgatgaatccaggaccagctatggccagtcacaaggaaggagaagaccgttttttccctttgtctggctctctaatatttggcaaacaagggacaggaagagacaacgtggatgcggataaagtaatgattacgaacgaagaagagattaaaaccaccaatcctgtggcaacagagtcctatggacaagtggcaacaaaccaccagagcgcccaagcacaggcgcagactggctgggtccaaaaccaggggatattacctggcatggtgtggcaagatcgtgacgtgtaccttcaaggacctatctgggcaaagattcctcacacggatggaaactttcatccttctcctctgatgggaggctttggaatgaaacatccgcctcctcaaatcctcatcaaaaatactccggtaccggcagatcctccgacggccttcaacaaggacaagctgaactcatttatcactcagtactccactggacaggtcagcgtggaaattgagtgggagctacagaaagaaaacagcaaacgttggaatccagagattcagtacacttccaactactacaagtctaataatgtggaatttgctgtaaatactgaaggtgtttatagtgaacctcgccctattggaacccggtatctcacacgaaacttgtaa 736 AA PAT source 1..736 mol_type protein organism adeno-associated virus hu96 virus adenoasociado hu96 MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPGYKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVEQSPQEPDSSEGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPSGVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQVFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRSSFYCLEYFPSQMLRTGNNFTFSYEFENVPFHSSYAHSQSLDRLMNPLIDQYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPIYRQQRVSTTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSGSLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQAQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGGFGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWELQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRNL 1509 DNA PAT source 1..1509 mol_type other DNA note Wild-Type hSGSH coidng sequence secuencia codificante de hSGSH natural organism Homo sapiens Homo sapiens atgagctgccccgtgcccgcctgctgcgcgctgctgctagtcctggggctctgccgggcgcgtccccggaacgcactgctgctcctcgcggatgacggaggctttgagagtggcgcgtacaacaacagcgccatcgccaccccgcacctggacgccttggcccgccgcagcctcctctttcgcaatgccttcacctcggtcagcagctgctctcccagccgcgccagcctcctcactggcctgccccagcatcagaatgggatgtacgggctgcaccaggacgtgcaccacttcaactccttcgacaaggtgcggagcctgccgctgctgctcagccaagctggtgtgcgcacaggcatcatcgggaagaagcacgtggggccggagaccgtgtacccgtttgactttgcgtacacggaggagaatggctccgtcctccaggtggggcggaacatcactagaattaagctgctcgtccggaaattcctgcagactcaggatgaccggcctttcttcctctacgtcgccttccacgacccccaccgctgtgggcactcccagccccagtacggaaccttctgtgagaagtttggcaacggagagagcggcatgggtcgtatcccagactggaccccccaggcctacgacccactggacgtgctggtgccttacttcgtccccaacaccccggcagcccgagccgacctggccgctcagtacaccaccgtcggccgcatggaccaaggagttggactggtgctccaggagctgcgtgacgccggtgtcctgaacgacacactggtgatcttcacgtccgacaacgggatccccttccccagcggcaggaccaacctgtactggccgggcactgctgaacccttactggtgtcatccccggagcacccaaaacgctggggccaagtcagcgaggcctacgtgagcctcctagacctcacgcccaccatcttggattggttctcgatcccgtaccccagctacgccatctttggctcgaagaccatccacctcactggccggtccctcctgccggcgctggaggccgagcccctctgggccaccgtctttggcagccagagccaccacgaggtcaccatgtcctaccccatgcgctccgtgcagcaccggcacttccgcctcgtgcacaacctcaacttcaagatgccctttcccatcgaccaggacttctacgtctcacccaccttccaggacctcctgaaccgcaccacagctggtcagcccacgggctggtacaaggacctccgtcattactactaccgggcgcgctgggagctctacgaccggagccgggacccccacgagacccagaacctggccaccgacccgcgctttgctcagcttctggagatgcttcgggaccagctggccaagtggcagtgggagacccacgacccctgggtgtgcgcccccgacggcgtcctggaggagaagctctctccccagtgccagcccctccacaatgagctgtga