LISTADO DE SECUENCIAS <110> Boehringer Ingelheim Vetmedica GmbH <120> COMPOSICIÓN INMUNÓGENA ÚTIL PARA LA VACUNACIÓN CONTRA EL ROTAVIRUS <130> 23-0015 <160> 25 <170> PatentIn versión 3.5 <210> 1 <211> 247 <212> PRT <213> Rotavirus <400> 1 Met Ala Ser Leu Ile Tyr Arg Gln Leu Leu Thr Asn Ser Tyr Thr Val 1 5 10 15 Asn Leu Ser Asp Glu Ile Gln Glu Ile Gly Ser Ala Lys Ser Gln Asp 20 25 30 Val Thr Ile Asn Pro Gly Pro Phe Ala Gln Thr Gly Tyr Ala Pro Val 35 40 45 Asn Trp Gly Ala Gly Glu Thr Asn Asp Ser Thr Thr Val Glu Pro Leu 50 55 60 Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Asn Pro Pro Thr Ser Tyr 65 70 75 80 Trp Val Leu Leu Ala Pro Thr Val Glu Gly Val Ile Ile Gln Gly Thr 85 90 95 Asn Asn Thr Asp Arg Trp Leu Ala Thr Ile Leu Ile Glu Pro Asn Val 100 105 110 Gln Thr Thr Asn Arg Ile Tyr Asn Leu Phe Gly Gln Gln Val Thr Leu 115 120 125 Ser Val Glu Asn Thr Ser Gln Thr Gln Trp Lys Phe Ile Asp Val Ser 130 135 140 Thr Thr Thr Pro Thr Gly Ser Tyr Thr Gln His Gly Pro Leu Phe Ser 145 150 155 160 Thr Pro Lys Leu Tyr Ala Val Met Lys Phe Ser Gly Arg Ile Tyr Thr 165 170 175 Tyr Ser Gly Thr Thr Pro Asn Ala Thr Thr Gly Tyr Tyr Ser Thr Thr 180 185 190 Asn Tyr Asp Thr Val Asn Met Thr Ser Phe Cys Asp Phe Tyr Ile Ile 195 200 205 Pro Arg Asn Gln Glu Glu Lys Cys Thr Glu Tyr Ile Asn His Gly Leu 210 215 220 Pro Pro Ile Gln Asn Thr Arg Asn Val Val Pro Val Ser Leu Ser Ala 225 230 235 240 Arg Glu Ile Val His Thr Arg 245 <210> 2 <211> 160 <212> PRT <213> Rotavirus <400> 2 Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Asn Pro Pro Thr Ser Tyr 1 5 10 15 Trp Val Leu Leu Ala Pro Thr Val Glu Gly Val Ile Ile Gln Gly Thr 20 25 30 Asn Asn Thr Asp Arg Trp Leu Ala Thr Ile Leu Ile Glu Pro Asn Val 35 40 45 Gln Thr Thr Asn Arg Ile Tyr Asn Leu Phe Gly Gln Gln Val Thr Leu 50 55 60 Ser Val Glu Asn Thr Ser Gln Thr Gln Trp Lys Phe Ile Asp Val Ser 65 70 75 80 Thr Thr Thr Pro Thr Gly Ser Tyr Thr Gln His Gly Pro Leu Phe Ser 85 90 95 Thr Pro Lys Leu Tyr Ala Val Met Lys Phe Ser Gly Arg Ile Tyr Thr 100 105 110 Tyr Ser Gly Thr Thr Pro Asn Ala Thr Thr Gly Tyr Tyr Ser Thr Thr 115 120 125 Asn Tyr Asp Thr Val Asn Met Thr Ser Phe Cys Asp Phe Tyr Ile Ile 130 135 140 Pro Arg Asn Gln Glu Glu Lys Cys Thr Glu Tyr Ile Asn His Gly Leu 145 150 155 160 <210> 3 <211> 168 <212> PRT <213> Rotavirus <400> 3 Asp Ser Thr Thr Val Glu Pro Leu Leu Asp Gly Pro Tyr Gln Pro Thr 1 5 10 15 Thr Phe Asn Pro Pro Thr Ser Tyr Trp Val Leu Leu Ala Pro Thr Val 20 25 30 Glu Gly Val Ile Ile Gln Gly Thr Asn Asn Thr Asp Arg Trp Leu Ala 35 40 45 Thr Ile Leu Ile Glu Pro Asn Val Gln Thr Thr Asn Arg Ile Tyr Asn 50 55 60 Leu Phe Gly Gln Gln Val Thr Leu Ser Val Glu Asn Thr Ser Gln Thr 65 70 75 80 Gln Trp Lys Phe Ile Asp Val Ser Thr Thr Thr Pro Thr Gly Ser Tyr 85 90 95 Thr Gln His Gly Pro Leu Phe Ser Thr Pro Lys Leu Tyr Ala Val Met 100 105 110 Lys Phe Ser Gly Arg Ile Tyr Thr Tyr Ser Gly Thr Thr Pro Asn Ala 115 120 125 Thr Thr Gly Tyr Tyr Ser Thr Thr Asn Tyr Asp Thr Val Asn Met Thr 130 135 140 Ser Phe Cys Asp Phe Tyr Ile Ile Pro Arg Asn Gln Glu Glu Lys Cys 145 150 155 160 Thr Glu Tyr Ile Asn His Gly Leu 165 <210> 4 <211> 167 <212> PRT <213> Secuencia artificial <220> <223> Secuencia de consenso de rotavirus <400> 4 Asp Ser Thr Thr Ile Glu Pro Val Leu Asp Gly Pro Tyr Gln Pro Thr 1 5 10 15 Ser Phe Lys Pro Pro Asn Asp Tyr Trp Ile Leu Leu Asn Pro Thr Asn 20 25 30 Gln Gln Ile Val Leu Glu Gly Thr Asn Arg Thr Asp Val Trp Val Ala 35 40 45 Leu Leu Leu Ile Glu Pro Asn Val Thr Asn Gln Ser Arg Gln Tyr Thr 50 55 60 Leu Phe Gly Glu Thr Lys Gln Ile Thr Val Glu Asn Asn Thr Asn Lys 65 70 75 80 Trp Lys Phe Phe Glu Met Phe Arg Asn Ser Ala Asn Ala Glu Phe Gln 85 90 95 His Lys Arg Thr Leu Thr Ser Asp Thr Lys Leu Ala Gly Phe Leu Lys 100 105 110 His Gly Gly Arg Val Trp Thr Phe His Gly Glu Thr Pro Asn Ala Thr 115 120 125 Thr Asp Tyr Ser Ser Thr Ser Asn Leu Ser Glu Ile Glu Thr Val Ile 130 135 140 His Thr Glu Phe Tyr Ile Ile Pro Arg Ser Gln Glu Ser Lys Cys Asn 145 150 155 160 Glu Tyr Ile Asn Thr Gly Leu 165 <210> 5 <211> 170 <212> PRT <213> Secuencia artificial <220> <223> Secuencia de consenso de rotavirus <400> 5 Asp Ser Thr Thr Val Glu Pro Val Leu Asp Gly Pro Tyr Gln Pro Thr 1 5 10 15 Thr Phe Asn Pro Pro Ile Glu Tyr Trp Thr Leu Phe Ala Pro Asn Asp 20 25 30 Lys Gly Val Val Ala Glu Leu Thr Asn Asn Thr Asp Ile Trp Leu Ala 35 40 45 Ile Ile Leu Ile Glu Pro Asn Val Pro Gln Glu Leu Arg Thr Tyr Thr 50 55 60 Ile Phe Gly Gln Gln Val Asn Leu Val Ile Glu Asn Thr Ser Gln Thr 65 70 75 80 Lys Trp Lys Phe Ala Asp Phe Arg Arg Arg Ser Gln Asn Asp Thr Tyr 85 90 95 Val Leu Asn Asp Thr Leu Leu Ser Asp Thr Lys Leu Gln Ala Ala Met 100 105 110 Lys Tyr Gly Ala Arg Leu Phe Thr Phe Thr Gly Asp Thr Pro Asn Ala 115 120 125 Ala Pro Gln Glu Tyr Gly Tyr Glu Thr Asn Asn Tyr Ser Ala Ile Glu 130 135 140 Ile Arg Ser Phe Cys Asp Phe Tyr Ile Ile Pro Arg Met Pro Arg Glu 145 150 155 160 Val Cys Arg Asn Tyr Ile Asn His Gly Leu 165 170 <210> 6 <211> 181 <212> PRT <213> Rotavirus <400> 6 Glu Ser Thr Phe Lys Ser Ser Asn Ile Thr Gly Pro His Asn Asn Thr 1 5 10 15 Val Ile Glu Trp Ser Asn Leu Met Asn Ser Asp Ile Trp Leu Leu Tyr 20 25 30 Gln Lys Pro Leu Asp Ile Thr Ala Pro Ile Arg Leu Leu Lys His Gly 35 40 45 Pro Glu Asn His Ala Asp Val Ala Ala Phe Glu Leu Trp Tyr Gly Lys 50 55 60 Ala Gly His Thr Val Thr Ser Ile Tyr Tyr Ser Ala Ile Ser Asn Pro 65 70 75 80 Asn Asn Thr Val Thr Leu Thr Ser Asp Ser Leu Val Leu Phe Trp Asn 85 90 95 Glu Gly Gln Thr Ile Leu Asp Thr Lys Thr Val Asn Phe Asn Trp Asn 100 105 110 Met Gly Gly Ile Leu Val Arg Pro Ser Arg Gly Thr Arg Val Asp Ile 115 120 125 Cys Met Ser Asp Met Asp Asn Thr Asp Gly Thr Asn Phe Asn Trp Ile 130 135 140 Gln Trp Lys His Glu Phe Pro Arg Ser Ser Ser Asn Ala Asn Val Ser 145 150 155 160 Met Tyr Val Glu Tyr Tyr Leu Ala Ser Ser Asp Pro Tyr His Glu Leu 165 170 175 Lys Glu Leu Gln Arg 180 <210> 7 <211> 229 <212> PRT <213> Sus scrofa <400> 7 Thr Lys Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro 1 5 10 15 Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 20 25 30 Ile Ser Arg Thr Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln 35 40 45 Glu Asn Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val 50 55 60 His Thr Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr 65 70 75 80 Arg Val Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly 85 90 95 Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile 100 105 110 Thr Arg Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln Val 115 120 125 Tyr Thr Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser 130 135 140 Ile Thr Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu 145 150 155 160 Trp Gln Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr 165 170 175 Pro Pro Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe 180 185 190 Ser Val Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala 195 200 205 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser 210 215 220 Lys Thr Pro Gly Lys 225 <210> 8 <211> 233 <212> PRT <213> Cavia porcellus <400> 8 Arg Thr Pro Gln Pro Asn Pro Cys Thr Cys Pro Lys Cys Pro Pro Pro 1 5 10 15 Glu Asn Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 20 25 30 Asp Thr Leu Met Ile Ser Leu Thr Pro Arg Val Thr Cys Val Val Val 35 40 45 Asp Val Ser Gln Asp Glu Pro Glu Val Gln Phe Thr Trp Phe Val Asp 50 55 60 Asn Lys Pro Val Gly Asn Ala Glu Thr Lys Pro Arg Val Glu Gln Tyr 65 70 75 80 Asn Thr Thr Phe Arg Val Glu Ser Val Leu Pro Ile Gln His Gln Asp 85 90 95 Trp Leu Arg Gly Lys Glu Phe Lys Cys Lys Val Tyr Asn Lys Ala Leu 100 105 110 Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Ala Pro Arg 115 120 125 Met Pro Asp Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Ser Lys 130 135 140 Ser Lys Val Ser Val Thr Cys Leu Ile Ile Asn Phe Phe Pro Ala Asp 145 150 155 160 Ile His Val Glu Trp Ala Ser Asn Arg Val Pro Val Ser Glu Lys Glu 165 170 175 Tyr Lys Asn Thr Pro Pro Ile Glu Asp Ala Asp Gly Ser Tyr Phe Leu 180 185 190 Tyr Ser Lys Leu Thr Val Asp Lys Ser Ala Trp Asp Gln Gly Thr Val 195 200 205 Tyr Thr Cys Ser Val Met His Glu Ala Leu His Asn His Val Thr Gln 210 215 220 Lys Ala Ile Ser Arg Ser Pro Gly Lys 225 230 <210> 9 <211> 3 <212> PRT <213> Secuencia artificial <220> <223> ligador <400> 9 Gly Gly Ser 1 <210> 10 <211> 8 <212> PRT <213> Secuencia artificial <220> <223> ligador <400> 10 Gly Gly Ser Gly Gly Ser Gly Gly 1 5 <210> 11 <211> 10 <212> PRT <213> Secuencia artificial <220> <223> ligador <400> 11 Ala Ser Gly Gly Gly Gly Gly Gly Gly Gly 1 5 10 <210> 12 <211> 401 <212> PRT <213> Secuencia artificial <220> <223> proteína de fusión <400> 12 Met Asp Ser Thr Thr Val Glu Pro Leu Leu Asp Gly Pro Tyr Gln Pro 1 5 10 15 Thr Thr Phe Asn Pro Pro Thr Ser Tyr Trp Val Leu Leu Ala Pro Thr 20 25 30 Val Glu Gly Val Ile Ile Gln Gly Thr Asn Asn Thr Asp Arg Trp Leu 35 40 45 Ala Thr Ile Leu Ile Glu Pro Asn Val Gln Thr Thr Asn Arg Ile Tyr 50 55 60 Asn Leu Phe Gly Gln Gln Val Thr Leu Ser Val Glu Asn Thr Ser Gln 65 70 75 80 Thr Gln Trp Lys Phe Ile Asp Val Ser Thr Thr Thr Pro Thr Gly Ser 85 90 95 Tyr Thr Gln His Gly Pro Leu Phe Ser Thr Pro Lys Leu Tyr Ala Val 100 105 110 Met Lys Phe Ser Gly Arg Ile Tyr Thr Tyr Ser Gly Thr Thr Pro Asn 115 120 125 Ala Thr Thr Gly Tyr Tyr Ser Thr Thr Asn Tyr Asp Thr Val Asn Met 130 135 140 Thr Ser Phe Cys Asp Phe Tyr Ile Ile Pro Arg Asn Gln Glu Glu Lys 145 150 155 160 Cys Thr Glu Tyr Ile Asn His Gly Leu Gly Gly Ser Thr Lys Thr Lys 165 170 175 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 180 185 190 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 195 200 205 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 210 215 220 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 225 230 235 240 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 245 250 255 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 260 265 270 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 275 280 285 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro 290 295 300 Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu 305 310 315 320 Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn 325 330 335 Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln 340 345 350 Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys 355 360 365 Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu 370 375 380 Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly 385 390 395 400 Lys <210> 13 <211> 400 <212> PRT <213> Secuencia artificial <220> <223> proteína de fusión <400> 13 Met Asp Ser Thr Thr Ile Glu Pro Val Leu Asp Gly Pro Tyr Gln Pro 1 5 10 15 Thr Ser Phe Lys Pro Pro Asn Asp Tyr Trp Ile Leu Leu Asn Pro Thr 20 25 30 Asn Gln Gln Ile Val Leu Glu Gly Thr Asn Arg Thr Asp Val Trp Val 35 40 45 Ala Leu Leu Leu Ile Glu Pro Asn Val Thr Asn Gln Ser Arg Gln Tyr 50 55 60 Thr Leu Phe Gly Glu Thr Lys Gln Ile Thr Val Glu Asn Asn Thr Asn 65 70 75 80 Lys Trp Lys Phe Phe Glu Met Phe Arg Asn Ser Ala Asn Ala Glu Phe 85 90 95 Gln His Lys Arg Thr Leu Thr Ser Asp Thr Lys Leu Ala Gly Phe Leu 100 105 110 Lys His Gly Gly Arg Val Trp Thr Phe His Gly Glu Thr Pro Asn Ala 115 120 125 Thr Thr Asp Tyr Ser Ser Thr Ser Asn Leu Ser Glu Ile Glu Thr Val 130 135 140 Ile His Thr Glu Phe Tyr Ile Ile Pro Arg Ser Gln Glu Ser Lys Cys 145 150 155 160 Asn Glu Tyr Ile Asn Thr Gly Leu Gly Gly Ser Thr Lys Thr Lys Pro 165 170 175 Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val Phe 180 185 190 Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 195 200 205 Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val 210 215 220 Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr 225 230 235 240 Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val 245 250 255 Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys 260 265 270 Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser 275 280 285 Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 290 295 300 His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val 305 310 315 320 Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly 325 330 335 Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp 340 345 350 Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala 355 360 365 Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala 370 375 380 Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 385 390 395 400 <210> 14 <211> 403 <212> PRT <213> Secuencia artificial <220> <223> proteína de fusión <400> 14 Met Asp Ser Thr Thr Val Glu Pro Val Leu Asp Gly Pro Tyr Gln Pro 1 5 10 15 Thr Thr Phe Asn Pro Pro Ile Glu Tyr Trp Thr Leu Phe Ala Pro Asn 20 25 30 Asp Lys Gly Val Val Ala Glu Leu Thr Asn Asn Thr Asp Ile Trp Leu 35 40 45 Ala Ile Ile Leu Ile Glu Pro Asn Val Pro Gln Glu Leu Arg Thr Tyr 50 55 60 Thr Ile Phe Gly Gln Gln Val Asn Leu Val Ile Glu Asn Thr Ser Gln 65 70 75 80 Thr Lys Trp Lys Phe Ala Asp Phe Arg Arg Arg Ser Gln Asn Asp Thr 85 90 95 Tyr Val Leu Asn Asp Thr Leu Leu Ser Asp Thr Lys Leu Gln Ala Ala 100 105 110 Met Lys Tyr Gly Ala Arg Leu Phe Thr Phe Thr Gly Asp Thr Pro Asn 115 120 125 Ala Ala Pro Gln Glu Tyr Gly Tyr Glu Thr Asn Asn Tyr Ser Ala Ile 130 135 140 Glu Ile Arg Ser Phe Cys Asp Phe Tyr Ile Ile Pro Arg Met Pro Arg 145 150 155 160 Glu Val Cys Arg Asn Tyr Ile Asn His Gly Leu Gly Gly Ser Thr Lys 165 170 175 Thr Lys Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro 180 185 190 Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 195 200 205 Arg Thr Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn 210 215 220 Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr 225 230 235 240 Ala Gln Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val 245 250 255 Val Ser Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu 260 265 270 Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg 275 280 285 Ile Ile Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr 290 295 300 Leu Pro Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr 305 310 315 320 Cys Leu Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln 325 330 335 Arg Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro 340 345 350 Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val 355 360 365 Asp Lys Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met 370 375 380 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr 385 390 395 400 Pro Gly Lys <210> 15 <211> 414 <212> PRT <213> Secuencia artificial <220> <223> proteína de fusión <400> 15 Met Glu Ser Thr Phe Lys Ser Ser Asn Ile Thr Gly Pro His Asn Asn 1 5 10 15 Thr Val Ile Glu Trp Ser Asn Leu Met Asn Ser Asp Ile Trp Leu Leu 20 25 30 Tyr Gln Lys Pro Leu Asp Ile Thr Ala Pro Ile Arg Leu Leu Lys His 35 40 45 Gly Pro Glu Asn His Ala Asp Val Ala Ala Phe Glu Leu Trp Tyr Gly 50 55 60 Lys Ala Gly His Thr Val Thr Ser Ile Tyr Tyr Ser Ala Ile Ser Asn 65 70 75 80 Pro Asn Asn Thr Val Thr Leu Thr Ser Asp Ser Leu Val Leu Phe Trp 85 90 95 Asn Glu Gly Gln Thr Ile Leu Asp Thr Lys Thr Val Asn Phe Asn Trp 100 105 110 Asn Met Gly Gly Ile Leu Val Arg Pro Ser Arg Gly Thr Arg Val Asp 115 120 125 Ile Cys Met Ser Asp Met Asp Asn Thr Asp Gly Thr Asn Phe Asn Trp 130 135 140 Ile Gln Trp Lys His Glu Phe Pro Arg Ser Ser Ser Asn Ala Asn Val 145 150 155 160 Ser Met Tyr Val Glu Tyr Tyr Leu Ala Ser Ser Asp Pro Tyr His Glu 165 170 175 Leu Lys Glu Leu Gln Arg Gly Gly Ser Thr Lys Thr Lys Pro Pro Cys 180 185 190 Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val Phe Ile Phe 195 200 205 Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Gln Val 210 215 220 Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu Val Gln Phe 225 230 235 240 Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln Thr Arg Pro 245 250 255 Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Pro 260 265 270 Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys Cys Lys Val 275 280 285 Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile Ser Lys Ala 290 295 300 Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro His Ala 305 310 315 320 Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu Val Ile Gly 325 330 335 Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 340 345 350 Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln Asp Val Asp 355 360 365 Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys Ala Ser Trp 370 375 380 Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu Ala Leu His 385 390 395 400 Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly Lys 405 410 <210> 16 <211> 579 <212> PRT <213> Secuencia artificial <220> <223> proteína de fusión <400> 16 Met Asp Ser Thr Thr Val Glu Pro Leu Leu Asp Gly Pro Tyr Gln Pro 1 5 10 15 Thr Thr Phe Asn Pro Pro Thr Ser Tyr Trp Val Leu Leu Ala Pro Thr 20 25 30 Val Glu Gly Val Ile Ile Gln Gly Thr Asn Asn Thr Asp Arg Trp Leu 35 40 45 Ala Thr Ile Leu Ile Glu Pro Asn Val Gln Thr Thr Asn Arg Ile Tyr 50 55 60 Asn Leu Phe Gly Gln Gln Val Thr Leu Ser Val Glu Asn Thr Ser Gln 65 70 75 80 Thr Gln Trp Lys Phe Ile Asp Val Ser Thr Thr Thr Pro Thr Gly Ser 85 90 95 Tyr Thr Gln His Gly Pro Leu Phe Ser Thr Pro Lys Leu Tyr Ala Val 100 105 110 Met Lys Phe Ser Gly Arg Ile Tyr Thr Tyr Ser Gly Thr Thr Pro Asn 115 120 125 Ala Thr Thr Gly Tyr Tyr Ser Thr Thr Asn Tyr Asp Thr Val Asn Met 130 135 140 Thr Ser Phe Cys Asp Phe Tyr Ile Ile Pro Arg Asn Gln Glu Glu Lys 145 150 155 160 Cys Thr Glu Tyr Ile Asn His Gly Leu Gly Gly Ser Thr Lys Thr Lys 165 170 175 Pro Pro Cys Pro Ile Cys Pro Ala Cys Glu Ser Pro Gly Pro Ser Val 180 185 190 Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 195 200 205 Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn Pro Glu 210 215 220 Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr Ala Gln 225 230 235 240 Thr Arg Pro Lys Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser 245 250 255 Val Leu Pro Ile Gln His Gln Asp Trp Leu Asn Gly Lys Glu Phe Lys 260 265 270 Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg Ile Ile 275 280 285 Ser Lys Ala Lys Gly Gln Thr Arg Glu Pro Gln Val Tyr Thr Leu Pro 290 295 300 Pro His Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr Cys Leu 305 310 315 320 Val Ile Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Gln Arg Asn 325 330 335 Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro Gln Gln 340 345 350 Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Phe Ser Val Asp Lys 355 360 365 Ala Ser Trp Gln Gly Gly Gly Ile Phe Gln Cys Ala Val Met His Glu 370 375 380 Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Lys Thr Pro Gly 385 390 395 400 Lys Gly Gly Ser Gly Gly Ser Gly Gly Asp Ser Thr Thr Val Glu Pro 405 410 415 Val Leu Asp Gly Pro Tyr Gln Pro Thr Thr Phe Asn Pro Pro Ile Glu 420 425 430 Tyr Trp Thr Leu Phe Ala Pro Asn Asp Lys Gly Val Val Ala Glu Leu 435 440 445 Thr Asn Asn Thr Asp Ile Trp Leu Ala Ile Ile Leu Ile Glu Pro Asn 450 455 460 Val Pro Gln Glu Leu Arg Thr Tyr Thr Ile Phe Gly Gln Gln Val Asn 465 470 475 480 Leu Val Ile Glu Asn Thr Ser Gln Thr Lys Trp Lys Phe Ala Asp Phe 485 490 495 Arg Arg Arg Ser Gln Asn Asp Thr Tyr Val Leu Asn Asp Thr Leu Leu 500 505 510 Ser Asp Thr Lys Leu Gln Ala Ala Met Lys Tyr Gly Ala Arg Leu Phe 515 520 525 Thr Phe Thr Gly Asp Thr Pro Asn Ala Ala Pro Gln Glu Tyr Gly Tyr 530 535 540 Glu Thr Asn Asn Tyr Ser Ala Ile Glu Ile Arg Ser Phe Cys Asp Phe 545 550 555 560 Tyr Ile Ile Pro Arg Met Pro Arg Glu Val Cys Arg Asn Tyr Ile Asn 565 570 575 His Gly Leu <210> 17 <211> 1206 <212> ADN <213> Secuencia artificial <220> <223> codifica una proteína de fusión <400> 17 atggatagta ccacagtaga accactgttg gacggcccct accaacctac tacgtttaac 60 cccccaacct catactgggt gttgctcgcg cctacagtcg agggtgtcat aatccaaggt 120 acgaataaca ccgatcgctg gttggctact atccttattg aaccgaatgt tcagaccaca 180 aatcgcattt acaacttgtt cggacagcag gtgacccttt cagttgagaa cacgagtcaa 240 acccaatgga aatttatcga cgtttctacc acaacaccta ccggcagcta tacgcaacac 300 ggcccactct ttagcacccc taagctgtat gcagtaatga agtttagcgg acgcatttac 360 acatactcag gtacaactcc taatgctact acgggctact atagcactac taattatgat 420 acggtgaata tgacaagttt ctgtgatttc tacatcatcc ccagaaacca ggaggagaaa 480 tgtactgaat atataaatca tggactgggc ggctcgacca agaccaagcc cccctgtcct 540 atttgcccgg catgcgagag tccgggtccg tccgtcttta ttttcccccc aaagccaaaa 600 gatacgctga tgatcagccg caccccccag gtcacatgcg tggttgtaga tgtaagccaa 660 gaaaatcccg aagtgcagtt ttcctggtat gtcgatggcg tggaggttca cacagcacag 720 acgcggccga aggaagagca gtttaattcc acgtaccgag tggtgagtgt gctgcctatt 780 cagcatcagg attggttgaa cggaaaggag ttcaagtgca aggtgaacaa caaagacctg 840 cccgcaccaa tcactcgcat aatcagcaag gctaagggac agacacgcga gccacaggtg 900 tacaccctgc caccgcatgc agaagagctg agccgatcca aggtatccat aacctgcctg 960 gtgatcggat tctacccccc cgatatcgac gtcgagtggc agaggaatgg ccagccggag 1020 ccagagggca actaccggac cacgcctccg cagcaggatg tcgatggtac atattttctg 1080 tacagcaaat ttagcgtgga caaggccagt tggcagggcg gcggtatctt ccagtgtgct 1140 gtcatgcacg aggcactcca caatcattac acccagaaat ccatttcgaa gacgccgggc 1200 aagtag 1206 <210> 18 <211> 1203 <212> ADN <213> Secuencia artificial <220> <223> codifica una proteína de fusión <400> 18 atggatagta ctactatcga accagttttg gatgggccat accagccaac atcgtttaaa 60 ccaccaaatg actattggat tttattgaac cctactaatc aacagattgt gctggaaggc 120 acaaacagaa cagatgtttg ggtcgcgctc ctgctaatag agccgaacgt aaccaaccag 180 tcccgccaat acaccctatt cggtgaaaca aagcaaataa ctgttgaaaa taacacgaat 240 aagtggaagt tttttgaaat gtttagaaac tcagcaaatg ccgagtttca gcataaacgg 300 acgttgactt ctgacaccaa gctagcgggt ttccttaaac atggcggccg cgtctggacg 360 ttccacggag agacccccaa tgccaccacc gactactcta gcacaagcaa tctctcagag 420 atagaaaccg taatacatac tgagttctat atcatacccc gaagtcaaga gtccaagtgc 480 aacgagtata tcaatacggg gttgggtgga tctaccaaaa ccaagccccc ctgccctatt 540 tgtccagcgt gcgagtcgcc gggtcccagt gtatttattt ttccaccgaa gcctaaagac 600 acacttatga tcagccgcac tccccaagtc acttgtgtgg tagttgacgt gagccaagaa 660 aatccggaag tccagttttc atggtatgtc gatggggtag aggtgcacac ggctcaaacg 720 aggcctaaag aagagcaatt caattcaaca tatcgtgtgg tctccgtcct tcctatacag 780 caccaagatt ggttaaacgg aaaagaattt aaatgtaaag tgaataacaa ggatctgccg 840 gctcccatta cgcggatcat tagcaaagca aagggacaga cgcgtgagcc tcaggtttac 900 actctaccgc cacatgcaga ggaactctcg cgaagtaagg tgtctataac gtgtctggta 960 attgggttct acccgcctga catcgacgtt gagtggcaga gaaacggtca gccggaaccg 1020 gagggcaact acaggacaac cccccctcag caagatgtag atggcacgta cttcctctat 1080 tctaagttct cggttgacaa ggcctcatgg cagggcggag ggatcttcca atgcgctgtg 1140 atgcatgaag cgttacacaa tcactataca caaaagtcca tatccaaaac tccaggaaaa 1200 tga 1203 <210> 19 <211> 1221 <212> ADN <213> Secuencia artificial <220> <223> codifica una proteína de fusión <400> 19 atgacttatc cgcgccgacg atatcgccga agaaggcata gaccgcggtc tcatctagga 60 caaatattgc ggcgccgccc atggttggta catcctcgtc atcgataccg ctggcggagg 120 aagaacggca tctttaatac taggctctcc cgaacgttcg ggtatacagt caaggctact 180 acagtcacca caccgagttg ggcagtggac atgatgcgtt ttaatatcga cgatttcgta 240 cctcccggtg ggggtactaa taaaatatcc attcccttcg agtactacag gatacgcaaa 300 gttaaggtgg aattttggcc ttgttcgcca attacgcaag gcgatagagg ggtcggctcg 360 accgccgtga ttctcgacga caacttcgtt acaaaagcta ctgcgttaac atatgatcca 420 tacgtcaact actcttcaag acacacgata cctcaaccat tttcctacca cagccgttat 480 ttcactccta agcccgtact agactccaca atagactact tccagcctaa taataagaga 540 aaccagttat ggcttagact tcaaacctca cggaacgttg accacgttgg cctaggcacg 600 gcctttgaga atagtaaata tgatcaggac tataacatcc gtgtaacgat gtacgtacag 660 tttcgcgagt ttaaccttaa agaccccccc ctcgagcccg gaggatcaga tagcacgaca 720 gttgaaccgg tgctggatgg gccatatcag cctaccacgt tcaatccacc aattgaatac 780 tggaccctct tcgcgccgaa cgacaaaggt gtggtagctg agttaactaa taatactgac 840 atctggttgg ctatcatcct gattgaacca aatgttccgc aggaattgcg tacctatact 900 attttcgggc agcaagtaaa ccttgtcata gagaatacaa gtcaaaccaa atggaagttt 960 gccgatttta gacgtcggtc tcagaatgat acgtatgtcc tgaatgatac gttactatct 1020 gatacaaaac tgcaagccgc aatgaagtat ggagcaaggc tatttacttt tactggagat 1080 accccgaatg cggcgccgca agaatatggt tacgaaacga ataactatag cgcaatagag 1140 attaggtcgt tttgtgactt ctacatcatt cccaggatgc ctcgggaagt gtgccgaaac 1200 tacataaacc acggtctttg a 1221 <210> 20 <211> 1245 <212> ADN <213> Secuencia artificial <220> <223> codifica una proteína de fusión <400> 20 atggaatcta cattcaaatc atcaaatata actggtccac acaataacac agtcattgaa 60 tggagtaatt taatgaattc tgatatttgg ttattgtatc aaaaaccatt ggatataact 120 gcaccaatca gattattaaa acatggaccg gaaaatcatg ctgatgtagc agcttttgaa 180 ttatggtatg gtaaagctgg tcataccgtg acatcaatat attattcagc aatatctaat 240 cctaataata ctgttacgtt aacgtcggat tcattagttc tattttggaa cgaaggtcaa 300 acgatactgg atacaaagac agtcaatttt aattggaata tgggtggtat attagttaga 360 ccgtcaagag gtacacgtgt ggacatttgt atgtctgata tggacaatac agatggtact 420 aattttaatt ggattcaatg gaagcatgag ttcccccgta gtagtagtaa tgctaatgtt 480 agtatgtatg ttgaatatta tctagcaagt agtgatccat accatgaact caaagagttg 540 caaagaggcg gctcgaccaa gaccaagccc ccctgtccta tttgcccggc atgcgagagt 600 ccgggtccgt ccgtctttat tttcccccca aagccaaaag atacgctgat gatcagccgc 660 accccccagg tcacatgcgt ggttgtagat gtaagccaag aaaatcccga agtgcagttt 720 tcctggtatg tcgatggcgt ggaggttcac acagcacaga cgcggccgaa ggaagagcag 780 tttaattcca cgtaccgagt ggtgagtgtg ctgcctattc agcatcagga ttggttgaac 840 ggaaaggagt tcaagtgcaa ggtgaacaac aaagacctgc ccgcaccaat cactcgcata 900 atcagcaagg ctaagggaca gacacgcgag ccacaggtgt acaccctgcc accgcatgca 960 gaagagctga gccgatccaa ggtatccata acctgcctgg tgatcggatt ctaccccccc 1020 gatatcgacg tcgagtggca gaggaatggc cagccggagc cagagggcaa ctaccggacc 1080 acgcctccgc agcaggatgt cgatggtaca tattttctgt acagcaaatt tagcgtggac 1140 aaggccagtt ggcagggcgg cggtatcttc cagtgtgctg tcatgcacga ggcactccac 1200 aatcattaca cccagaaatc catttcgaag acgccgggca agtag 1245 <210> 21 <211> 1740 <212> ADN <213> Secuencia artificial <220> <223> codifica una proteína de fusión <400> 21 atggacagca cgacggttga accgctactg gacggaccat accaacctac tacatttaat 60 cctccaacaa gttattgggt tctgcttgct cctacagtcg agggagtgat aatacagggg 120 acaaataata cggaccggtg gttagctaca attttgatcg aaccgaatgt gcaaacgact 180 aatcgcatat acaatttatt cggccaacag gtaaccttaa gtgtggagaa cacctcgcaa 240 actcaatgga aatttattga tgtctccaca acaaccccca ctggatcgta cactcaacac 300 gggccgctct tctctacccc taaactatac gcggttatga aatttagtgg gagaatctac 360 acttattctg gtactacacc caatgcgacc actggttact attcaaccac aaactacgac 420 actgtcaata tgacgtcctt ttgtgatttt tatataatcc ctaggaatca agaggagaag 480 tgtacggaat acattaacca tggtctgggg ggcagcacga aaacaaaacc gccatgcccc 540 atctgccctg cctgcgaaag tcccggccct tccgttttca tttttccccc aaagcccaag 600 gacacgctaa tgatttccag gacaccacag gtcacgtgtg tggttgtgga tgttagccag 660 gagaatccgg aagttcagtt ttcgtggtat gtagatgggg tagaagtgca tacagcccag 720 acgcgaccaa aagaagagca gttcaacagc acctatcgtg ttgtaagtgt attaccgata 780 caacaccaag actggcttaa tggtaaagag ttcaaatgca aggtaaacaa taaggatcta 840 ccggcgccta taacgagaat catttcaaag gctaagggac aaacaaggga gccgcaagtg 900 tacaccttgc ccccccacgc cgaggaattg agtaggtcaa aagtctcgat aacttgtttg 960 gttatagggt tctatcctcc agacatcgat gtggaatggc aacggaacgg gcaacccgaa 1020 cctgaaggca actatcgcac taccccaccg caacaggatg tcgacggtac ttattttttg 1080 tactccaagt tttctgtaga caaggcatca tggcagggcg gaggtatttt tcaatgtgct 1140 gtcatgcatg aagcactcca caaccattac acccagaaat ctatttcaaa gacacccgga 1200 aaaggcggat cagggggatc aggaggcgat agcactacgg tcgagccggt tctggacgga 1260 ccttatcagc ctaccacttt taatcccccg atagaatatt ggaccctctt tgcaccaaac 1320 gacaagggcg tcgtagcaga gctaacgaac aacaccgata tctggttagc aattatcctc 1380 atcgagccga acgtaccaca ggaattacgg acgtacacca tcttcgggca gcaagtcaac 1440 ctcgtgattg aaaacacgtc ccagacgaag tggaaattcg cggacttccg tagacgttcg 1500 caaaacgata cgtatgtgct gaatgatact cttctatcgg acactaagct tcaggccgct 1560 atgaaatacg gcgcccgact cttcacattc actggtgata cacccaacgc cgcgccacag 1620 gagtatggtt acgagaccaa taactatagc gcaatcgaga ttcgctcttt ttgtgatttc 1680 tatataatac cacgaatgcc ccgtgaggta tgccgaaatt atatcaatca tggtctttga 1740 <210> 22 <211> 25 <212> ADN <213> Secuencia artificial <220> <223> secuencia de cebador <400> 22 gctagggaya aaattgttga aggta 25 <210> 23 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> secuencia de cebador <400> 23 attggcaaat ttcctattcc tcc 23 <210> 24 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> secuencia de sonda <400> 24 atgaatggaa atgaytttca aac 23 <210> 25 <211> 23 <212> ADN <213> Secuencia artificial <220> <223> secuencia de sonda <400> 25 atgaatggaa ataattttca aac 23